LOCUS       NC_011900              14083 bp    DNA     circular CON 11-FEB-2024
DEFINITION  Streptococcus pneumoniae ATCC 700669, complete sequence.
ACCESSION   NC_011900
VERSION     NC_011900.1
KEYWORDS    RefSeq; complete genome.
SOURCE      Streptococcus pneumoniae ATCC 700669
  ORGANISM  Streptococcus pneumoniae ATCC 700669
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1
  AUTHORS   Croucher,N.J., Walker,D., Romero,P., Lennard,N., Paterson,G.K.,
            Bason,N.C., Mitchell,A.M., Quail,M.A., Andrew,P.W., Parkhill,J.,
            Bentley,S.D. and Mitchell,T.J.
  TITLE     Role of conjugative elements in the evolution of the
            multidrug-resistant pandemic clone Streptococcus pneumoniaeSpain23F
            ST81
  JOURNAL   J Bacteriol 191 (5), 1480-1489 (2009)
   PUBMED   19114491
REFERENCE   2  (bases 1 to 2221315)
  AUTHORS   Croucher,N.J.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-SEP-2008) Croucher N.J., Pathogen Genomics, Wellcome
            Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton,
            Cambridge, Cambridgeshire, CB10 1SA, UNITED KINGDOM
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/11/2024 00:47:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,268
            CDSs (total)                      :: 2,195
            Genes (coding)                    :: 2,039
            CDSs (with protein)               :: 2,039
            Genes (RNA)                       :: 73
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 156
            CDSs (without protein)            :: 156
            Pseudo Genes (ambiguous residues) :: 0 of 156
            Pseudo Genes (frameshifted)       :: 82 of 156
            Pseudo Genes (incomplete)         :: 70 of 156
            Pseudo Genes (internal stop)      :: 58 of 156
            Pseudo Genes (multiple problems)  :: 45 of 156
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:23:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 463460
            Orig. end                         :: 477543
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            FM211187.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..14083
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..14083
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..14083
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:9083]"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..9083
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(334..663)
                     /locus_tag="SPN23F_RS02435"
                     /old_locus_tag="SPN23F04760"
                     /old_locus_tag="SPN23F_04760"
     CDS             complement(334..663)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /locus_tag="SPN23F_RS02435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04760"
                     /old_locus_tag="SPN23F_04760"
                     /product="hypothetical protein"
                     /protein_id="WP_000132572.1"
                     /transl_table=11
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGFL
                     AKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLILNE
                     NQRAN"
     misc_feature    complement(707..725)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 23.85"
                     /tool="antismash"
     misc_feature    802..815
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.71"
                     /tool="antismash"
     gene            939..1277
                     /locus_tag="SPN23F_RS02440"
                     /old_locus_tag="SPN23F04770"
                     /old_locus_tag="SPN23F_04770"
     CDS             939..1277
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /locus_tag="SPN23F_RS02440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04770"
                     /old_locus_tag="SPN23F_04770"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_000876675.1"
                     /transl_table=11
                     /translation="MKYMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEEASFNML
                     QNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYREIR
                     QKWLKEGE"
     gene            1282..2019
                     /locus_tag="SPN23F_RS02445"
                     /old_locus_tag="SPN23F04780"
                     /old_locus_tag="SPN23F_04780"
     CDS             1282..2019
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /locus_tag="SPN23F_RS02445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04780"
                     /old_locus_tag="SPN23F_04780"
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="WP_001219120.1"
                     /transl_table=11
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLLA
                     EVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQVS
                     ALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYYLET
                     SPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKLLFFP
                     NGGSCLIARYKVREVSEAINKLH"
     gene            2033..3373
                     /locus_tag="SPN23F_RS02450"
                     /old_locus_tag="SPN23F04790"
                     /old_locus_tag="SPN23F_04790"
     CDS             2033..3373
                     /EC_number="2.7.13.3"
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /locus_tag="SPN23F_RS02450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04790"
                     /old_locus_tag="SPN23F_04790"
                     /product="sensor histidine kinase"
                     /protein_id="WP_001017642.1"
                     /transl_table=11
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILLA
                     FVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRGVS
                     YFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQKSLT
                     QINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKEKLYE
                     RLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIKEVYGS
                     VLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEIQVEGMS
                     LLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGIDISEIFSF
                     GASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(3444..3572)
                     /gene="blpC"
                     /locus_tag="SPN23F_RS02455"
                     /old_locus_tag="SPN23F04791"
                     /old_locus_tag="SPN23F_04791"
     CDS             complement(3444..3572)
                     /codon_start=1
                     /gene="blpC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /locus_tag="SPN23F_RS02455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04791"
                     /old_locus_tag="SPN23F_04791"
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="WP_000358815.1"
                     /transl_table=11
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(3629..4990)
                     /locus_tag="SPN23F_RS02460"
                     /old_locus_tag="SPN23F04800"
                     /old_locus_tag="SPN23F_04800"
     CDS             complement(3629..4990)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /locus_tag="SPN23F_RS02460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04800"
                     /old_locus_tag="SPN23F_04800"
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="WP_001069060.1"
                     /transl_table=11
                     /translation="MNPNLFRSVEFYQRRYHNYATVFIIPLSLLFTFILIFSLVATKEI
                     TVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTALAT
                     QLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTNTEV
                     SNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRYLVAS
                     QGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVLRTQFL
                     QTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRIPTGTEI
                     AQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQTIDQTPT
                     RTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILTHSD"
     gene            complement(5001..6677)
                     /locus_tag="SPN23F_RS02465"
                     /old_locus_tag="SPN23F04810"
                     /old_locus_tag="SPN23F_04810"
     CDS             complement(5001..6677)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR01193"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /locus_tag="SPN23F_RS02465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04810"
                     /old_locus_tag="SPN23F_04810"
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="WP_196300924.1"
                     /sec_met_domain="TIGR01193 (E-value: 5.8e-264, bitscore:
                     874.8, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIFKQKSLIAYIVLSSLLVTIINIGGSYYLQGILDEYIPNQMKST
                     LGIISVGLVITYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTG
                     EIISRFTDANSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFII
                     FSFMKPFEKMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKS
                     FKLSKYSILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLFSYFTTPMEN
                     IINLQTKLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDT
                     LTDINLTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRR
                     HINYLPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSD
                     GAGLSGGQKQRIALARALLTKAPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAH
                     RLSIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            complement(6571..7158)
                     /locus_tag="SPN23F_RS14160"
                     /old_locus_tag="SPN23F04820"
     CDS             complement(6571..7158)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /locus_tag="SPN23F_RS14160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04820"
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="WP_000205166.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 1.3e-41, bitscore:
                     138.6, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKTN
                     KEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQTK
                     KDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPSSDF
                     QTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            7440..7637
                     /gene="blpI"
                     /locus_tag="SPN23F_RS02475"
                     /old_locus_tag="SPN23F04830"
                     /old_locus_tag="SPN23F_04830"
     CDS             7440..7637
                     /codon_start=1
                     /gene="blpI"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /locus_tag="SPN23F_RS02475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04830"
                     /old_locus_tag="SPN23F_04830"
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="WP_001093259.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.1e-10,
                     bitscore: 37.3, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAATG
                     PITGGAATLICVGSGIMSSL"
     gene            8104..8373
                     /gene="blpJ"
                     /locus_tag="SPN23F_RS02480"
                     /old_locus_tag="SPN23F04840"
                     /old_locus_tag="SPN23F_04840"
     CDS             8104..8373
                     /codon_start=1
                     /gene="blpJ"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /locus_tag="SPN23F_RS02480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04840"
                     /old_locus_tag="SPN23F_04840"
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="WP_001093248.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 2.1e-18,
                     bitscore: 63.5, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITGG
                     TGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            8442..8690
                     /gene="blpK"
                     /locus_tag="SPN23F_RS02485"
                     /old_locus_tag="SPN23F04850"
                     /old_locus_tag="SPN23F_04850"
     CDS             8442..8690
                     /codon_start=1
                     /gene="blpK"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /locus_tag="SPN23F_RS02485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04850"
                     /old_locus_tag="SPN23F_04850"
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="WP_000379965.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 8.9e-11,
                     bitscore: 39.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAALG
                     CAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     misc_feature    complement(8911..8926)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: strong,
                     score: 18.42"
                     /tool="antismash"
     gene            8934..9083
                     /gene="blpO"
                     /locus_tag="SPN23F_RS02490"
     CDS             8934..9083
                     /codon_start=1
                     /gene="blpO"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /locus_tag="SPN23F_RS02490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="WP_001809846.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 3.7e-10,
                     bitscore: 37.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDGC
                     ATTV"
     gene            9187..9306
                     /locus_tag="SPN23F_RS02495"
                     /old_locus_tag="SPN23F04880"
                     /old_locus_tag="SPN23F_04880"
     CDS             9187..9306
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /locus_tag="SPN23F_RS02495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04880"
                     /old_locus_tag="SPN23F_04880"
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="WP_000346296.1"
                     /transl_table=11
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     misc_feature    complement(9442..9455)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.08"
                     /tool="antismash"
     gene            9826..10145
                     /locus_tag="SPN23F_RS02510"
                     /old_locus_tag="SPN23F04910"
                     /old_locus_tag="SPN23F_04910"
                     /pseudo=""
     CDS             9826..10145
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826248.1"
                     /locus_tag="SPN23F_RS02510"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04910"
                     /old_locus_tag="SPN23F_04910"
                     /product="immunity protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MLVSTYLFLFRQFSNFF"
     gene            10774..11157
                     /locus_tag="SPN23F_RS02520"
                     /old_locus_tag="SPN23F04930"
                     /old_locus_tag="SPN23F_04930"
     CDS             10774..11157
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /locus_tag="SPN23F_RS02520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04930"
                     /old_locus_tag="SPN23F_04930"
                     /product="hypothetical protein"
                     /protein_id="WP_000877381.1"
                     /transl_table=11
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGVF
                     LLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHIFI
                     VFNALMYLLSSSQVSLKKNKLSS"
     gene            11209..11898
                     /locus_tag="SPN23F_RS02525"
                     /old_locus_tag="SPN23F04940"
                     /old_locus_tag="SPN23F_04940"
     CDS             11209..11898
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /locus_tag="SPN23F_RS02525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04940"
                     /old_locus_tag="SPN23F_04940"
                     /product="CPBP family intramembrane glutamic endopeptidase"
                     /protein_id="WP_000760532.1"
                     /sec_met_domain="Abi (E-value: 2e-12, bitscore: 44.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFVW
                     IQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKHVQ
                     STAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDLLLP
                     SILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLPFLLT
                     FLHRVLG"
     gene            11940..12173
                     /gene="blpZ"
                     /locus_tag="SPN23F_RS02530"
                     /old_locus_tag="SPN23F04941"
                     /old_locus_tag="SPN23F_04941"
     CDS             11940..12173
                     /codon_start=1
                     /gene="blpZ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /locus_tag="SPN23F_RS02530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04941"
                     /old_locus_tag="SPN23F_04941"
                     /product="immunity protein BlpZ"
                     /protein_id="WP_000276498.1"
                     /transl_table=11
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFNF
                     LNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            12324..12935
                     /locus_tag="SPN23F_RS02535"
                     /old_locus_tag="SPN23F04950"
                     /old_locus_tag="SPN23F_04950"
     CDS             12324..12935
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /locus_tag="SPN23F_RS02535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04950"
                     /old_locus_tag="SPN23F_04950"
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_000394044.1"
                     /sec_met_domain="Abi (E-value: 1.4e-19, bitscore: 67.4,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSLL
                     VTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHLAE
                     VSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSNVPS
                     FIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            13096..13890
                     /locus_tag="SPN23F_RS02540"
                     /old_locus_tag="SPN23F04960"
                     /old_locus_tag="SPN23F_04960"
     CDS             13096..13890
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) APH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /locus_tag="SPN23F_RS02540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SPN23F04960"
                     /old_locus_tag="SPN23F_04960"
                     /product="phosphotransferase family protein"
                     /protein_id="WP_000363002.1"
                     /sec_met_domain="APH (E-value: 4.4e-18, bitscore: 63.1,
                     seeds: 156, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGLA
                     REQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQLS
                     RLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDVRHS
                     NWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTVLSKL
                     YWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     misc_feature    complement(14053..14067)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: weak, score: 17.11"
                     /tool="antismash"
ORIGIN
        1 acaggaggta gttcgaggat tgaccagttt tgttttactt gtggaattag ttgttgggtt
       61 gattaccttc caagaaaaac tagcccttct agccttacta ggagctggtt tggttttact
      121 agtcttgtat ttgccttatc aggtaaaacg tcagatgcag gactaacatt gctgatacga
      181 cactaaaaaa gaagttgagt tcagtctgtc tcaacttctt ttttgttact acaggataat
      241 ggttggtccg tagagactta tactcttcga aaatctcttc aaaccacgtc agcgtcgtct
      301 taccgtactc aagtacagct tgcggctagc ttcctagttt gctctttgat tttcattgag
      361 tattaacttg gtcttgactt ggtcaaagtg gaagcggtca taggcccgcc aagcggcgcg
      421 agttggagca tctggatcaa gagcgctgag tcccatgaga agactggaag tctggtaaaa
      481 tttttctagt tcaatcaaga atcgattatc cactgtttca gccttggcta gaaaaccaag
      541 aatagagttt aattgctcct gaaagcggac gtcgtcagcg cttgcctgtt tgcatgcttg
      601 gtaggctttg tttaagtcag taatcaaagt atgagctctt ttgatggggt ctgtatctgt
      661 catgggaatg cctcctttaa tctgggtgcc agtcttactt ctggcaactg tgttttgata
      721 ctgttagttt atcactttta attctttttt tttattcaaa tctttaattg tcattgaaat
      781 gtcttgaatt gcgctgagtg aattttatga taaaatagtt gtaagctcat catgatgttg
      841 tagaaaataa tccttttagg agttttcaaa gactgtttag gattgggtgt gcttgggcta
      901 gaccttttct gttattcttt tcttaggagg agaatccaat gaaatatatg attattcaga
      961 cgcagaaaac agtctataaa gtaaacatcg acgatatcta ctatatccaa acacatccaa
     1021 ctaaagccca taccgtacag attgttacag aagaagctag ttttaatatg cttcaaaatt
     1081 taagtaatct tgagaaccaa tgtggggaaa ccttgatgag atgtcatcga aattgtttgg
     1141 ttaatcttga taaattaaaa tcgattgatt ttcaagaaag aatccttttt ctcggagaag
     1201 aaggtcaata cgctgtcaag tatgccagac gtcgctatag agaaattcgt caaaaatggt
     1261 tgaaagaggg agagtaagaa gatgagaata tttgttttag aggatgattt ttcccaacag
     1321 actagaattg aaacgacgat tgagaaactt ttgaaagcac atcatatcat tcctagctct
     1381 tttgaggtat ttggcaagcc ggaccaactg ctggctgaag tgcatgagaa gggggcccat
     1441 cagctattct ttttggatat tgagattcga aatgaagaga tgaagggact ggaagtggct
     1501 agaaagattc gggatcggga tccttatgcc ctgattgtct ttgtgacgac tcactcggag
     1561 tttatgcccc tgtcttttcg ctaccaagtg tctgctttgg actacattga taaggccttg
     1621 tcagcagagg agtttgaatc tcggatcgag acagccctcc tctatgccaa tagtcaagat
     1681 agtaaaagtc tggcggaaga ttgcttttac tttaaatcaa aatttgccca atttcagtat
     1741 ccttttaaag aggtttacta tctcgaaacg tcgcccagag cccatcgtgt tattctctat
     1801 accaagacag acaggctgga atttacagcg agtttagagg aggttttcaa gcaggagccc
     1861 cgtctcttgc agtgccaccg ctcttttctc atcaatcctg caaatgtggt gcatttggat
     1921 aagaaagaaa aactgctttt ctttcccaat ggtggaagtt gtctaatcgc ccgttataag
     1981 gtcagggaag tgtctgaggc catcaataaa ttacactgag ctaggagaat ttatgaacat
     2041 tgcttggata ttgttgtata cacttgttac taatggacta gaaattgtca tcttctttaa
     2101 ggtggatgga attgatctta ctttcgagag gatttttaag gcctttcttt ttaagatact
     2161 gttggccttt gtttttgtga tgattagcta tatagtagga aatgtttacc tatcttattt
     2221 tatggaaccc ttgtacggca taggcttatc tttcttatta ttgagagggc ttcctaaaaa
     2281 actccttttc ttttatggtc tctttccaat gatattggtg aatctctttt atagaggagt
     2341 ctcctatttt gtgcttccat ttttgggaca agggcaagta tatgatggct actcatttac
     2401 tgggttatgt ataataattt tcaatttctt catttctcta gcttttttga aatggttgga
     2461 ctacgatttt actagcttga gaaaggagat tctcgataaa gcctttcaaa agtccctgac
     2521 tcagattaac tggataatgg gtggctacta tctagtcatg gaaagtctgt ctttttttga
     2581 atatgaacaa agtattcaat caaagactgt tcgccatctc atcctagtct tttacctact
     2641 cttttttatg ggggttatca agaaattgga tacctatttg aaggaaaaac tctatgagag
     2701 attggaacaa gagcaggccc tgcgctatag agatatggaa cgctatagtc ggcatataga
     2761 ggagctttac aaggaagtac ggagctttcg tcatgattat accaacctct tgactagctt
     2821 acgtctgggc attgaagagg aggatatgga gcagataaaa gaggtctatg gctccgtctt
     2881 aaaggattcc agtcagaaat tgcagaacaa taaatatgac ctgggcagat tggtaaatat
     2941 tcgtgataaa gccctcaaaa gtcttctagc agggaaattt ttaaaagcca gagataagaa
     3001 cattgtcttt aatgtcgaag ttcctgagga gattcaggtc gaggggatga gtctacttga
     3061 ttttctaacc attgtgtcta tcctttgtga caatgctatt gaagccagtg tggaagctag
     3121 tcaacctcat gtttcaatcg cctttttaaa aaatggagca caggagacct ttatcatcga
     3181 aaactccatc aaagaagagg gcatcgatat ttctgaaatc ttctcctttg gagccagttc
     3241 taaaggggag gagagaggag ttggtctcta taccgtcatg aagattgtgg aaagccatcc
     3301 taataccagt ctaaatacca cctgtcaaaa tcaagtcttt cgtcaggtac ttactgtgat
     3361 acatacagaa tgatgaaaaa taagaccgag agtacttgtt tctcggtctt atttttttat
     3421 agttgtattg gatgatgaag ttcttatgtg atgtaatgag catatctatt aatgttatat
     3481 aataaatctt cccacaatcc tccacctgta atttggttga gttcggtagt tgttagttct
     3541 tgaaatgaag ttaggttttg tttcttatcc atgttatgat tctccttttt gataagataa
     3601 taaatagtta tagagtgtta tctgaaaatt aatcagaatg ggttaaaatt ttatctttga
     3661 aataatcaaa atatgttttc tttgtagtta cactagtgac gcgaccttgt aagccatatt
     3721 ggatgagttt actatcctcg ttagatagtt ttgcaagagc ggttaattta aagagatttc
     3781 cttgctctgt tctggtagga gtttgatcaa ttgtctgaag ttggccgatg atggtggtgc
     3841 cgtgatttcc aatcttctcc agttttaatc ttacagtttg tcctttatct agtagaggta
     3901 gatagtcaga agatacgtag taagtgatta gtacttctct tgtatctgtg atgacaggga
     3961 atatttgagc aatttctgta ccagttggaa ttctattttt accttcaaat tcgctgttca
     4021 gatgaacgat acctttactt ggggaggtta aggtattgtt ttccaaacgc tgtgtggctt
     4081 gatctagttg tacttttaat tctgttaatt gattctccac agttagttgt tgctgtgagg
     4141 ctgtctgtaa aaactgagtg cggagtactt caattttggt tgctaaactg ttatcataag
     4201 ttgctacact tccgatacca gcttgctgaa ttttgaggct tgcgatagat gattcaagac
     4261 ctgcaatact ttgattaatt tgagataaaa atggctcctc tgcagttcct tgtgtttgtc
     4321 cttgtgaggc tacaagataa cgattcaaaa ttgactggtg cggattgcca gttggtaagc
     4381 gtgctctgtt atttatgata gcatctctca actcttgata ttctccaatt tgttgttgaa
     4441 cttttgtaat ttcttgttca atagctgatg aactgctatt ggaaagatta gcttgatttg
     4501 aaacttcggt gttagtcttt gtgataccca gttcaatatc atgggattgt ttagtaaaat
     4561 tcataaaggt attatggtag ccaaattcat cctcgccaga aaaaagatca gtcgcttttt
     4621 ctaagctttg tttcaaaatt ccaagtcctt ctttttgctt ctcaagtctt tgtaattgag
     4681 ttgctaaggc agttttctga ctttcttcca ttgtttcaga gtatttgatg agtaagtccc
     4741 ctttttcaac tacttgattt gccactaaat gattagctag gataggatta tcactggttg
     4801 actgaatgga ggcaatgaca cttgtagggg cgatttctcc ttgggaagta acagtaattt
     4861 cttttgtggc aacaagggag aaaatcaaga tgaaagtaaa tagtaatgaa agaggtataa
     4921 tgaacactgt cgcatagtta tggtaacgtc tctgataaaa ctcgacgctt ctaaaaagat
     4981 taggattcat gacattctcc ttatttattg aatagatgat ggtagaaacc ttgcgcctgc
     5041 attaactctt ggtgactacc aacttcaatg attttcccct ggtcaagaac aatgacacgg
     5101 ttggttcgtt cggctatact gagacgatgg gctacaaaga gaatggtttt atcagttaga
     5161 gacataagat tatctataac ctttttctca gtcaagacat caagaccgct agtagcttcg
     5221 tctagtatta aaacaggagc tttagttaaa agagcacgag cgagggctat tcgttgcttt
     5281 tgtcctcctg atagaccagc tccatcagag agctgagttt gatagcccat aggcattctt
     5341 tcaatgtctt gacgaatttc agctaattca caagctctta gaatatcttc ttggctaatc
     5401 atatgattac cgcccaaagt taagttttcc aagatagagc cattaaagat ataggcttgt
     5461 tgtggtaggt aattaatatg acggcgcaag acttttttat caatgttttt aatatcctga
     5521 tgattgatag aaatatgtcc tttgtagggt tcaaagaaat tgacaatcat tttggctaaa
     5581 gttgttttac cagaaccact aactccaact aggctaacct tatctccttg tttaatcgtg
     5641 agattaatat ctgttaaggt atctcgtcca aaaccatact tataagaaag gtcatcaaat
     5701 tcaatatcgc ccatcaaaaa atgtgaatgg acagggtttt cttgaacttg aaattcagat
     5761 tcgactagat agacttcgtt caaacggtta ttagcgacct tcgcagattg gagtttggtt
     5821 tggaggttga taatattttc cataggagtt gtaaagtaag aaaaaagtgt gttaaaggta
     5881 atcagctgac cgatagaaat tttactcgac atgactaatt gagcgccaaa ccataggata
     5941 aggatattca gaactaattt tgttccctgc tttaaactcg tttgtaaaat agaatattta
     6001 ctgagcttaa aggatttttc caaataatct acaaattcgc tgtctatatt ttgatagcga
     6061 ttttcttcac tcgtgagcga ctttatagtt tcaatcccgt tgatatcttc gataatggca
     6121 gagctaacca tagaattact ttgcatgaca tcatggttca ttttttcgaa aggtttcata
     6181 aaagaaaaga tgatgaacat gtatatagga atggaaataa gagaaagaag gaagagatta
     6241 gggttttgtg ccagtaagac gcctcctaca agaatcagaa tagaaacatc cagaaaaaga
     6301 gaaagaatgg tagaagccaa ggcatctata atagagttag catctgtgaa tcgtgaaatg
     6361 atttctcctg tacgacgtgt cgcaaagaaa gacatgggaa gttcaaaaat atggcgaata
     6421 taggataaaa tcacatcaat acttaatctc tgactcagaa cggttaggag ataatctctg
     6481 gagaagctca tgacttgttg gaggatatag gtgataacca gaccaactga gatgattcct
     6541 aaagttgatt tcatctgatt tggaatgtat tcatccaaga ttccttggag atagtaagaa
     6601 ccacctatat tgataatagt gaccaataag cttgagagaa caatgtaagc aatgagagat
     6661 ttttgtttga aaatcagagg aaggaagctt gcttagtaga ccattctttt tatctttatg
     6721 gggttgatag ctgggtttgg tagctagaaa aatagctact ccagtccatt catagaaaaa
     6781 gcgttctttt gacattttag tgatttttac agaagggtca ggatcaccaa taatcagata
     6841 gtctttcttt gtttgataga caacatagta atgttggagt tttccttctt tgttaacgtg
     6901 aacgataaat ggatagggga catcactcat gtcaaagagc gttttatctg cttgaacagg
     6961 tcttgtttca aagcccattt catcagcggc ttttacaatg ccaagagcag tcgtcccttc
     7021 tttattggtc tttgcaagtt ctctcaagtg agctagagaa aaatctgaac catagaattt
     7081 agcaatcgag gctaaggcag cgacaccaca gtctctcgca tctatttgag gaacaaatgt
     7141 acgtttataa gaagtcattg gcaattcctt tcatatagtg gataggtcta ttatgtcata
     7201 aaactaagtt catattttat gaaatcttga atagtcatta aaacttcctg aatggtaaaa
     7261 aagtgattag aaattatttt ttttaaacat ttagaggtgg cttgaaataa aaaacctaat
     7321 tcaagacgtt tcgatgacaa ttcaagatct ggatgaaaaa ttttaaaaaa cgatgatata
     7381 ctaaacttat caaagttaca acaagacaaa aataaaaaaa gaaaaaagga gtatttgtca
     7441 tgaatacaaa aatgatggaa caattttctg ttatggataa tgaagaactc gaaatagtta
     7501 gtggaggaag aggcaattta ggatctgcaa tcggtggttg tattggagca gtactattag
     7561 ctgctgcgac tggtccgata actgggggag cagcaacact tatttgtgta ggttcaggaa
     7621 ttatgtcctc tttgtaagat tatgattctt aaatatagta ttattatcgc tattaattta
     7681 ctgtcttatt tacttactta caaaatatca aagttatcta agaatcatga gaataagata
     7741 gttagcaaaa ttttaattat attatcaatc gtttatgtaa tcgttgatgc tttacttagt
     7801 tgatggattg gtaggaataa aaaacttaac tataaactat tatttactaa tattttttgc
     7861 tttctgcaag ttaataaata gtatagtaaa actttttatt gcaaggagaa taacgaaatt
     7921 aagatatttt agatactcaa gattattgaa atcttagact aaaaaatagg tactgctacc
     7981 tttaagaaga tagtagacgt atatactttt ttaagaaaat caaaaagata ctaaaaaaaa
     8041 tctatttgtt tattgaattt aagactttgg taacaaattg aaaataaaaa ggaggtattc
     8101 atcatgaata caaaaatgtt gtcacagtta gaggttatgg atactgaaat gcttgcgaaa
     8161 gttgaagggg ggtatagctc cactgattgt caaaatgcac tgattacagg agtcactacg
     8221 ggaattataa ctggtggaac tggagcaggt ttggccactt taggagtagc tggacttgct
     8281 ggtgcatttg ttggagcaca cattggtgca attgggggtg gattaacatg cttaggaggt
     8341 atggttggtg ataaattggg actaagttgg taagacttat agtaaaatta tgaggattct
     8401 attctgattt attttagttt tttaaaaaag gagtatttgt catggataca aaaatgatgt
     8461 cacaattttc tgttatggat actgaaatgc ttgcttgcgt tgaaggtggc ggatgcaatt
     8521 ggggagattt tgccaaagca ggtgttggag gagcagctgt agttgctgct ttaggttgtg
     8581 cagcgggtgg agtgaaatat gggaaaattc taggaccatg gggcgctgca ataggaggaa
     8641 ttggaggagc agtggtttgt ggatatttag cctataccgc tacatcataa aaatgaaagc
     8701 taaattttat gaaggctata ttctttatta ttctcttcgc ttttcaaacc tatcttattt
     8761 atctatccat aagtatctct gataaaaaac aaaagactat tgaattaaca tcattgaatt
     8821 gttttgtcat attatttcta atctatgata aattaatttt tctctttatt gcctatgttt
     8881 ttttgataat ttttatatta aacttgttcc gtaactgaaa agtgacatac tatatgaata
     8941 caaaaatgat gtcacaattt tctgttatgg ataatgaaat gcttgcttgc gttgaaggtg
     9001 gagatattga ttggggaaga aaaattagtt gtgcagcagg ggttgcatat ggcgcaattg
     9061 atgggtgtgc aacaacggtt tgacatttct attgggacca tttgctatag gaataggtgt
     9121 aactggtgct gcaggtggag ctatacttgg aggtgtagcc tatgcagcaa catgttggtg
     9181 gtaattatgg attttaaaag ttttattatt ggtttagtag ttggtatatt tggtccttat
     9241 atggatgact taattagaaa aatattttca aaatcttcca aaaaggatac ggatagtact
     9301 ctctaaaaaa taaatttctc ccctgtattt gctagcttaa tttacagggg agtttcttta
     9361 atattgtttc gcctgtcaaa atgatatgtg atagcaggat gaaaagtcta caaacaagtt
     9421 ggtatcaata caatataaag aggaaagtat gtttttcatg cttgcttttt taatttttac
     9481 aattcaagag gttttgatga ccatttatga tttgagtgat ccaaggtcaa aataagtgct
     9541 atactagcag tgtaaatgtt cttgctcaac taagataaat cgcacattta ggaggaaaac
     9601 ttatgaagaa aaaaatactg attattttcg ttctgtatct gatcatgtcc atctttcttt
     9661 atccgcttag ggagagtatt tggtataatc tattttatac catagcctat atgattgcgg
     9721 ttatgatcta tttttcttta attaaaaaga aagaaaagaa atgaaagatt ttcttgatta
     9781 aaaagaatca tattctttgt gagattgttc ctgggtcagt tgcctttgct tgtttctact
     9841 tatctatttc tatttcgtca gttttccaat ttcttttagt agttattaac ttgacttcta
     9901 ttttggttac tgtttatctt actagggaaa tgagattaag agagtttgaa gatgatgatt
     9961 tggttagtcc tagaaccaat caactcatgt ttatcggctt gacaggcttt atgtctatta
    10021 tttgtttgta tagaggtatc acagcaggag aatcttatca acaactaatc gcttatattg
    10081 gtgctattct ttgcttgatc atcatgcttc tactcatttg gggtttgaag tattataaaa
    10141 agtaggggct aatgaagttt ttgcaattta ggaggtttcg atgacaattc aagatttgga
    10201 tgaaaaattt taaaaaacaa tgatatacta aacttgtcaa agttgcaacg ggtgctgcta
    10261 cattatgtta aaaatcgctc ttgattgata agttggattt gttaggaaag gagtaaataa
    10321 ttatggataa gaaaaagata gtttcgacta taatatgtat agtatttctt gtagtgtcag
    10381 tagataattt ttttagagac ttaaccccgt tactgttcat tttaaatatt attggcctgt
    10441 cttgcttttc agtgctaaca tacattaaca taaaagagat actcttaaat ataagtaaat
    10501 gatgatttgg ttagtcctag aaccaatcaa cttatgttta tcggcttggc agattttatg
    10561 tttgtcattt gtttgtatag aggtatttca gaaacagaat tctatcaaca actaatcgct
    10621 tatataggtg tattctctgc ttgtttatca cgcttctgct catgtggggc ttgaagtatt
    10681 ataaaaagta gctttctatc cttttctgac aggatatttg agtagaaggc tctattaaat
    10741 tgttagtgaa ataaatgaat ggaggtattt aatatgaaat acagattatt ttttgttatt
    10801 ttcttgagta gtgtgttgga tattctttta gggacatttt tacaaatctc tatcgtatct
    10861 attgggtggc ttgttcttta cagtggtttg tttgaagcag gagttttcct tcttgctaat
    10921 aaaggggtgg cggtaaaaat caaggaagta gatattcgaa atcgctttaa atttattttt
    10981 ggaaaaacct tatggtttca aattcttttg ctcatctttt tgataatcaa actttatctt
    11041 ggtttggatg cgaggttgat tttattctat ggacatattt tcattgtctt taatgcctta
    11101 atgtatctat tatctagtag tcaggttagc cttaaaaaaa acaaactgtc ttcttaatct
    11161 tacagcagta gagcctgaca aggtgaggtc atagtaggga ggatttctat gaaaaagtat
    11221 caacttctat tcaaaataag tgcagttttc tcttacttat ttttcgtatt tggtctttct
    11281 cagatgacgc ttattattca aaattattgg caattttctt cccagattgg caatttcgtc
    11341 tggattcaaa atttcttgag tttgctattt agcggagtca tgatttggat tctggttaag
    11401 acaggtcatg gttatctctt tcacattcca agaaaaaaat ggctttggta ttcgattttg
    11461 acagtattag tggtagtgct ccagatctct tttaacgttc agacagctaa acatgttcag
    11521 tcaactgctg aaggttgggc tgtattgatc ggttatagtg ggaccaactt tgctgagcta
    11581 ggtatctata taactttgtt ctttctgact ccacttatgg aagagctaat ctatagagga
    11641 ttactgcaac acgccttttt taagcattcg agatttggcc ttgatttgct tcttccgtca
    11701 attttatttg ctcttcctca tttttcaagc ctgcctagtt tgttagatat cttcatcttt
    11761 gcaacatctg gcatcatctt tgctagtttg acccgctata ccaagagcat ttatccttcc
    11821 tatgcggtgc atgtgatcaa taatattttc gcaacattac catttttgct gactttttta
    11881 catagggtct tggggtaaaa aaacaaaagg cttgcttttc agccatagag gagattatca
    11941 tgtataaaca cttatttttc ctagattcca aaactttaga tcggttgaca ccctatattc
    12001 tagtcttggc ttctgacacc attgctttta atgtttttgt gctaaccttt gtatctgcgg
    12061 tggtttttaa tttcctaaat tccatgctag ctttaatggc tatattcata ggggctggct
    12121 atgtggtcgg attttggtta ctaatactca atgaaaatca aagagcaaac taggaaacta
    12181 gccgcaggct gtacttgagt acggcaaggc gacgttgacg tggtttgaat ttgattttcg
    12241 aagagtataa aatggtttgt tttggaaagg ctagaactaa agaatgacgc gtaggaagtc
    12301 tgtttggtga ggaggatagt tttatggagt tttttgataa atttcatgcc ttgtgttttg
    12361 gatttttagt actaataatt gtcattacag ttccttatac gattaaccat gggggttttt
    12421 ttcaaaatga atctgcattg attcttgtaa gtcttcttgt aacctcgctg agtgttgctt
    12481 atgctagaaa gtttgaaatg atttcttttg ggatgttaag caagaaacaa cttttgcttt
    12541 tcattgcaat ctttcttcta agtgtacttg agacgctggt ttatattcat ttcttcgctg
    12601 tttcttctgg ctcaggggtc caacacttgg cggaagtcag cagaggaatt tccctgtctt
    12661 tgattttgac tacctcagtt tttggcccca tccaggagga actcattttc agaggacttc
    12721 ttcaaggtgc ggtttttgac aattcttggt tagggcttgt gctaacttcc tctctctttt
    12781 ctttcatgca tggaccttct aatgtccctt cgtttatttt ttatctactt gggggtttgt
    12841 tgctgggctt tgcttataaa aagagtcaaa acctatgggt ttctactcta gtccacatgt
    12901 tttacaacag ttggccactc ctatattatt tataaaaatt atgaaaaggt aagcagaaga
    12961 tggtgcttac ctttttcaag ctataatgat acccaagcca atccagaggc ttttctttga
    13021 gaatcgggtt agagtggttg acgaataggc caaaaactag tagaatagta aggaaacttt
    13081 atacggagga aagaaatgga tttgggtgat aatgagctaa cactgactcc catacctggg
    13141 aaaagtggca aggcttatat gggtagctat cctgacggga agcgcatctt tgtaaaaatg
    13201 aacacctctc caatcctacc tggtctagct agagaacaaa ttgctccaca attattatgg
    13261 agtcgtcgtt tggcagatgg gcgtgatatg tgtgctcaag aatggttgac aggcaagata
    13321 ttgaccccct atgatatgaa tcgtaagcaa atcgtcaata ttttaacccg tcttcatcgc
    13381 tcacgtccgt tgatgacaca attgagtcgt ttgggctatg ccatggaaac acctgtagat
    13441 ttactacagt cttggcagga aacggctcca gatgctttgc gtaaaaatca ttttatcagt
    13501 gaagtgatgg ctgatttacg tcagactatt ccaggattta gagaggacca tgcgaccatt
    13561 gtccatggag atgtacgaca tagtaattgg attgagacag atagtggctt gatttattta
    13621 gtagattggg attcggttcg cttgaccgat cgcatgtttg atgtggccca tatgctctgc
    13681 cattatattt cagaacatca gtggaaggaa tggttgacct actacggtta caagtacaat
    13741 caaacggtat taagtaaatt gtattggtat ggtcaattgt cttatttgag tcagatttcc
    13801 aagtattata tgaaccaaga tttagaaaat gtcaatcggg agattcatgg tttgcgtcat
    13861 ttccgagaca agtatggaaa gagaagatga gagttagaaa tcgtaaaggg gcaacagaat
    13921 tactagaggc aaatccccag tatgtggtcc tcaatccctt ggaagccaag gcaaaatggc
    13981 gggacttgtt tggcaatgat aatcccattc atgtggaagt tggaagtgga aagggtgcct
    14041 ttgtttcagg tatggccaag caaaaccctg acatcaacta tat
//
