LOCUS       NC_013657              10150 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Lactococcus lactis subsp. lactis KF147 plasmid pKF147A, complete
            sequence.
ACCESSION   NC_013657
VERSION     NC_013657.1
KEYWORDS    RefSeq.
SOURCE      Lactococcus lactis subsp. lactis KF147
  ORGANISM  Lactococcus lactis subsp. lactis KF147
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 37510)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B., Wels,M., van Hijum,S.A.,
            Molenaar,D. and van Hylckama Vlieg,J.E.
  TITLE     Complete genome sequence of Lactococcus lactis subsp. lactis KF147,
            a plant-associated lactic acid bacterium
  JOURNAL   J Bacteriol 192 (10), 2649-2650 (2010)
   PUBMED   20348266
REFERENCE   2  (bases 1 to 37510)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B.A.M., Wels,M., van
            Hijum,S.A.F.T., Molenaar,D. and van Hylckama Vlieg,J.E.T.
  TITLE     Complete genome sequence of Lactococcus lactis ssp lactis KF147
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 37510)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B.A.M., Wels,M., van
            Hijum,S.A.F.T., Molenaar,D. and van Hylckama Vlieg,J.E.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) NIZO Food Research, Kluyver Centre for
            Genomics of Industrial Fermentation, PO Box 20, Ede 6710 BA, The
            Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000025045.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 01:08:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,639
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,493
            CDSs (with protein)               :: 2,493
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 25 of 55
            Pseudo Genes (incomplete)         :: 23 of 55
            Pseudo Genes (internal stop)      :: 14 of 55
            Pseudo Genes (multiple problems)  :: 5 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:59:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4384
            Orig. end                         :: 14534
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP001835.1.
            Bacteria source: Dr. W.J. Kelly, Agresearch Limited, New Zealand;
            william.kelly@agresearch.co.nz.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10150
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10150
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10150
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5150](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5150
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(167..1633)
                     /locus_tag="LLKF_RS12840"
                     /old_locus_tag="LLKF_p0005"
     CDS             complement(167..1633)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425922.1"
                     /locus_tag="LLKF_RS12840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_p0005"
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_012898731.1"
                     /transl_table=11
                     /translation="MEQLKLNQYFDYSLEPRRAILFQDVKSNYASIECVQRNLNPLTTS
                     LCVMSRADHSKGLTLASSPTFKKVFGMKNVSRASDLPFLIETRKFNYPQWYRTHTDIHG
                     QRTEPTLQYVAFIESWAKRTWIVPPQMQLYVDYKIEVTDILTNYTSIDEIHSYSIDESF
                     LDITESLNFFYSEIKNRYEQMNRIALDLQREIRDKLGLYVTVGMGDNPLLAKLAMDNYA
                     KHNDNMRALIRYEDVPNKLWTIPKMTDFWGIGKRTEKRLNKLGITSIKELANADPLLLK
                     QNLGTIGLQHFFHANGIDESNVREKYVPKTTSFSNSQILPRDYHKQREIELVIKEMAEN
                     LAIRLRKGGKLASNLSLYAGAASTSEYSSVKVSRNIEATQNTKELQDLAISLFREKYQG
                     GAIRQIGISGNQLSDSSVKQLSLFESVEENQTNKKQGLLQKVIDEIRETFDFLSIQKAS
                     SLSEGSRVIYRNKLIGGHAASQEREEKDVS"
     misc_feature    complement(1841..1859)
                     /note="TFBS match to DmdR1, Iron(II)-dependent repressor,
                     confidence: weak, score: 24.54"
                     /tool="antismash"
     gene            2006..2668
                     /locus_tag="LLKF_RS12845"
                     /old_locus_tag="LLKF_p0006"
                     /pseudo=""
     CDS             2006..2668
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /locus_tag="LLKF_RS12845"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_p0006"
                     /product="IS6 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MASKYRC"
     misc_feature    2807..2821
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 22.96"
                     /tool="antismash"
     gene            2956..3567
                     /locus_tag="LLKF_RS13080"
                     /old_locus_tag="LLKF_p0007"
     CDS             2956..3567
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012898732.1"
                     /locus_tag="LLKF_RS13080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_p0007"
                     /product="hypothetical protein"
                     /protein_id="WP_050749812.1"
                     /transl_table=11
                     /translation="MKINDGEKLVMKLSKVHCKECGGILNLDIVSHIKNQKLVCPYCQS
                     LYIYEAKYSEIGAELEADIELIRLKEEKENIKEFWKFKKLKEDHKVGFISLLILFSIPL
                     IGFLVMTTNYLIVHRPGQIELPISEKKLHGKNYKNVELKFEDMGFENIKYEKVRDLKLG
                     LFAHSGDVSEVTINGDNDFKKGDNYNKKSKIKIYYHVFPK"
     misc_feature    3735..3751
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 24.78"
                     /tool="antismash"
     gene            3990..4151
                     /locus_tag="LLKF_RS13665"
     CDS             3990..4151
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LLKF_RS13665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_153927130.1"
                     /transl_table=11
                     /translation="MNKINTIAILKAPKIIVLVESDLVSLENLAIITALRMKHRNIKAK
                     NIVGEMPT"
     misc_feature    complement(4158..4167)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     misc_feature    4926..4940
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence:
                     medium, score: 23.79"
                     /tool="antismash"
     gene            5001..5150
                     /locus_tag="LLKF_RS13325"
     CDS             5001..5150
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_process="GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF016272.4"
                     /locus_tag="LLKF_RS13325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="lactococcin family bacteriocin"
                     /protein_id="WP_017865277.1"
                     /sec_met_domain="Lactococcin (E-value: 7.7e-13, bitscore:
                     39.7, seeds: 3, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MENQTNFEVVSDEELSKINGGVALELTREGYYNQYGGPLLYENRP
                     LLEY"
     gene            5261..5965
                     /locus_tag="LLKF_RS12865"
                     /old_locus_tag="LLKF_p0010"
     CDS             5261..5965
                     /GO_function="GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abi"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017865276.1"
                     /locus_tag="LLKF_RS12865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_p0010"
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="WP_012898735.1"
                     /sec_met_domain="Abi (E-value: 2.7e-09, bitscore: 28.7,
                     seeds: 823, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKTIKKYKLNFFYLLLFVLLHTLYKIPGYLVFAYYLDNPLILPFT
                     DFLYTNSALAFPILLTLVAVIVIIIPYYLGFFKNTFEGWSTIRVFRLLVILFVLLLLIY
                     FTNVLMIRLGFSSSNHVGRSQNYLITMPKVMQYYIIGIVAPIIEEILWRVFLFKAVGNK
                     NLALFVSWPLFAFAHTGLSLEIIPYLFLSFIVTWAYYRNENVLESIFIHASYNLLSPLS
                     AQFLNSFFHFIV"
     gene            complement(6196..6792)
                     /locus_tag="LLKF_RS12870"
                     /old_locus_tag="LLKF_p0011"
     CDS             complement(6196..6792)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133553.1"
                     /locus_tag="LLKF_RS12870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_p0011"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_012898736.1"
                     /transl_table=11
                     /translation="MELSTILVEERKKKGITQQEVADTLYITRQSLSNWENGKNFPDIP
                     MLVELSKYYDFSLDIIKGDAQLMNQVQKDYELINTKKANKKYSVLLVILTALIVLTSVV
                     LIPLVSNNKILLKSFTLFDLLLCIVLIHTAVKFSKIVYQYYEGIPNSPLWVPKVFGYGI
                     SINPYNKFGKIITIALLVILDLFFIGVGITMIFFG"
     gene            7102..7521
                     /locus_tag="LLKF_RS12875"
                     /old_locus_tag="LLKF_p0012"
     CDS             7102..7521
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338443.1"
                     /locus_tag="LLKF_RS12875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_p0012"
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="WP_012898737.1"
                     /transl_table=11
                     /translation="MNHFKGKQFQKDVIIISVGYYLRYNLSYRDVQEMLYDRGINVSHT
                     TIYRWVQEYGKLLYQNGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASPTIK
                     GMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGIPD"
     gene            complement(8487..9110)
                     /locus_tag="LLKF_RS12880"
                     /old_locus_tag="LLKF_p0014"
     CDS             complement(8487..9110)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749140.1"
                     /locus_tag="LLKF_RS12880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_p0014"
                     /product="DUF2812 domain-containing protein"
                     /protein_id="WP_012898739.1"
                     /transl_table=11
                     /translation="MKKFRIFLSLKKEEEWINSIQEEGYKLVSVNSAVPMYTFEKLSTK
                     EMFIPYVRLDFREKSMKKTNYEDYVTLFSDSGWKLIKGSRSGGAQYFQQKRPDVSRGIF
                     SDKDSQESAKRRYVKYGYSYGFLFLLYFYIFSRNTSWTQIINFKSWYLTPNIWNLKGGS
                     FWYAFLFETPFALFRVLPLFFFLFLGIYYLIRSLINDDSKFKRK"
     gene            complement(9122..9433)
                     /locus_tag="LLKF_RS12885"
                     /old_locus_tag="LLKF_p0015"
     CDS             complement(9122..9433)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291321.1"
                     /locus_tag="LLKF_RS12885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_p0015"
                     /product="PadR family transcriptional regulator"
                     /protein_id="WP_012898740.1"
                     /transl_table=11
                     /translation="MSRNPHLPLTETTFYILLVLTTPAHGYAIIQEIEKLSNGFVKVAA
                     GTMYGAIENLLKLNWIEEIPNREKRRRVYKITDIGEEVLTLEIERLRSLIKLSSEFGY"
     misc_feature    complement(9461..9479)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 25.57"
                     /tool="antismash"
ORIGIN
        1 ataaagcact agatgatgtg gtaaagcttg aattttctta tcctcttttt tttctttttc
       61 aaaagtattt tgagcctccg tgagttctcc agtcgcaaat gcgccccatt tcatcatccc
      121 tctatcgtga taagcacgga tgacttcata ggggctatat gaacggtcaa ctgacatctt
      181 tttcctccct ctcttgactt gctgcatgac ctccaatgag tttgttccga taaatgacac
      241 gtgacccctc ggataaacta cttgcttttt gtatcgatag aaaatcgaat gtttctctaa
      301 tctcatcaat aactttttgg agtaatccct gttttttatt agtttgattt tcttcaacac
      361 tttcaaataa agaaagttgt ttaacagaac tatcagagag ttgatttccg cttattccta
      421 tttgtcttat cgctcccccc tggtactttt cacgaaataa ggagattgcg aggtcttgca
      481 attcttttgt gttttgcgtt gcttcaatat tgcgagaaac cttaacagaa gagtattcag
      541 aagtagaagc agcacctgca taaagtgaaa gattgcttgc tagttttccg ccctttctca
      601 atcttatcgc taagttctca gccatttctt taatcacaag ttcaatttct ctttgcttat
      661 gatagtcacg tggcagaatt tgagaattgc tgaagcttgt tgttttagga acatattttt
      721 ctctcacatt gctttcgtcg ataccgttag catggaaaaa atgttgaagt ccaattgttc
      781 ccaaattttg tttaaggaga agtgggtcag cattcgcaag ctctttgata gaagtgattc
      841 caagtttgtt taatcgtttc tcagttcgct ttccaattcc ccagaaatct gtcatcttag
      901 gaattgtcca tagtttatta gggacatctt cataacggat tagggctctc atattatcat
      961 tgtgcttggc atagttatcc attgcaagtt tggcaagcaa ggggttgtct cccatcccaa
     1021 cagtcacata tagtcctagt ttatctcgaa tctcacgttg caagtctaaa gcaattcgat
     1081 tcatctgttc ataacgattt ttaatttcag agtagaagaa gttcaatgac tctgtgatat
     1141 ccaaaaaaga ttcatcaatc gagtaggagt gaatctcatc aattgacgta taattggtca
     1201 gtatatctgt cacttcaatt ttatagtcca catagagctg catttgtgga ggaacaatcc
     1261 acgtgcgttt cgcccaagac tcaataaaag cgacatattg caaagttggt tccgttcgct
     1321 gtccatgaat gtctgtatgt gttcgatacc attgcggata attgaacttt cgtgtttcta
     1381 taagaaaagg taaatcgctg gcacgactga catttttcat accaaaaact tttttaaagg
     1441 ttggactaga ggcaagcgtt aaccctttag aatgatctgc tcgactcatg acacaaagag
     1501 aagtagtcag aggatttaaa tttcgctgaa cgcattcaat tgaagcataa ttagacttga
     1561 catcttgaaa gaggatggca cgacgtggtt caagcgagta gtcaaagtat tgatttaatt
     1621 ttaattgttc catagttcct cctaaatcaa aaatatcagt tttactgata ttatatcttt
     1681 ttttagagtg aaaaacaatc gaaataaaca ccaaaactga taacttggaa tatttaaggg
     1741 aaattcttag tccttcaaca tccttcctat gcttttggtt taatagagtt aagcatatta
     1801 tcatttgaaa gagaacgtgg agaaataatg gataaatact taaggcgagc ctatcagaga
     1861 atgaattaaa tgtcttttgg aggacaggcg ttggcttggt tcctttcaat aaggttatca
     1921 gatttggttt taaaaaaatt gaaaatatcg gttctgttgc aaagttttcc aaaaaatcta
     1981 ttctagtgca aaaaggtaga aaaagatagc gagtaagtat agatgctaac aattcaaaaa
     2041 agacgtcatt attgtcgctg ttggttacta cctgcgttac aatctaagct atcgtgaagt
     2101 tcaggaattg ttatatgatc gtggaataaa tgtttgtcat actaaaattt atcgttgggt
     2161 gcaagagtac agcaaagtcc tctattatct ttggaagaag aaaaatagac aatccttcta
     2221 ttcatggaaa atggacgaaa cctatatcaa aattaaggga cgttggcatt atctttatcg
     2281 tgcaattgat gcggacggct taaccttaga tatctggtta cgaaaaaaac gggatacgca
     2341 agcagcctat gctttcttaa aacgactcca taaacagttt ggtgagccga aagcaattgt
     2401 gaccgataaa gcaccttctc ttggctccgc ctttagaaag ttacagagtg tgggtttata
     2461 tactaagaca gagcaccgaa ctgtgaagta tcttaacaat ttaatagaac aagaccatcg
     2521 acctattaaa cgacgaaata aattttatca aagtctccgt acagcctctt ccacgattaa
     2581 gggcatggag accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg
     2641 cttttctgtg tccactttta tagtatagct ccaatgctta gtggtttaag attaggatag
     2701 cacgacttat ttattttcca atgaaatcaa ctagcaattc tggtatacta gatataattg
     2761 agtatcaaaa agagcagata aaagatttac aaaacactaa aggctcgaat caatttgaaa
     2821 agctacggca agaacatata ctatcgctct tcatcaacgt aagttagatt tgttgaaaga
     2881 acagaaaaaa ggctacttac aaaaatgttt gtataggaga agtcctagta tcaatttgtt
     2941 tagaaaataa taagattgaa aattaatgat ggggaaaaat tagtaatgaa attatcaaaa
     3001 gtacattgta aagaatgcgg aggaatatta aatttagata tagtatcaca cattaaaaat
     3061 caaaaattgg tttgccctta ttgccaatcg ctttatatct atgaagctaa atattcagag
     3121 attggtgccg aattagaggc tgatattgag cttatcagat taaaagagga aaaagaaaac
     3181 attaaagaat tctggaagtt caaaaaacta aaggaagatc ataaagtagg atttatcagt
     3241 ttacttattt tattcagtat tcctttaatt ggttttctag ttatgactac gaattatttg
     3301 atcgttcata gacctggtca aatagaatta cctataagtg aaaaaaaact acatggtaaa
     3361 aattataaaa acgtagaatt aaaatttgaa gatatgggtt ttgaaaatat aaaatatgaa
     3421 aaagtgagag atttaaaact tggattattt gctcattcag gggatgtttc tgaggtaaca
     3481 attaacggag acaatgattt taagaaaggt gataattaca ataaaaaatc aaaaataaaa
     3541 atatattatc atgtttttcc aaaatagatt taatgatgta tagggtctat aattatataa
     3601 taacccctag gaaataccat taaaacaacc cctataatct ttagttcata ttacaggggt
     3661 tgtttttctg tttattgata taatagttct taaaaataga ggtgatattt tgaaagtgaa
     3721 ttggttaaag ttgattggaa tttttttacc tttaaaatga ttcatattta ctgtcttcaa
     3781 ttgctttttt aaaaatttta aactaaatac aagtattgat aaacttttca aatatagtat
     3841 aaaagtacta actgattcta tcttgattaa tgcattacta ttccgaattt aagaacttga
     3901 cttaatatca taataaaaaa cctccaaaag tttaaatttt ggaggtcata taagatatat
     3961 gtttatttta tttgcttact gtggttagaa tgaataaaat aaatactata gctatactaa
     4021 aagcacctaa aataatagta ttagttgagt cagacttagt atctttagaa aacttagcta
     4081 ttatcactgc acttagaatg aaacatagaa atataaaagc taaaaacatt gttggagaga
     4141 tgccaacttg aaatttctca aaaaacaaat gataaataga agtaactaca actcctaaca
     4201 ttcctattat ataaagtagg gttacgagaa aatttaaatt aattaattga tgatcgagat
     4261 ttttattagt catacttaaa agactttcct taaaaaattt attatattta tattctaaca
     4321 taataaaatt tgctctgttg cgaaatttcc aagttccaaa attttagtgt aaaatgaata
     4381 ataagttagt gtatctataa tatattgttc acctatatac tttaccaatt gcaagaacat
     4441 aaaaaatact accaataaat gcaactatta accccagtaa tagcatccaa ataatatttt
     4501 ctccattgcg aattagcaga ggaagaaaaa atcctatcat agatattaat ggtgtatatg
     4561 ttcttttgta ataaggctcc caatttaata ttaaagaaat caaacaaatc aaaactccta
     4621 ataggtagac gataattaac aaaggtgaat tgagttgaga gaaattacca ttaagttgaa
     4681 gtaacccttt tggtaaaatt gcactcatct tattatagta attaatatca ttactatatc
     4741 taaccaaatc taatttataa aaagtggaat taaacatgat ataaattact gtaacaaccg
     4801 cagaaaagat tgaatagatg agcagacacc aacgttttag attaataaga ttgatgtaca
     4861 tagttcttac gccctttctt atattttgga taatatcagt atactccaaa aaaaaaatat
     4921 acaagaattt tcaaacaatt gttgcatgcc tttaatttta agagtatact gatattattg
     4981 aatataaatg agatttacat atggaaaatc aaacaaattt tgaagtagtt tcagatgaag
     5041 aactttctaa aattaatgga ggggtagctt tggaacttac tcgtgaaggt tactataatc
     5101 aatatggggg cccacttctt tatgaaaacc gtcctttgtt agaatattga ttattaaatg
     5161 acaagtcagt agtcttgcaa ccgtcgaatt aattgttagt gaattaagta ttggacagga
     5221 aatagattat ctatttcttg ttttttaggt gaagtattat atgaaaacta taaaaaaata
     5281 taaattaaat tttttttatt tattgttatt tgttctccta catacactct ataagattcc
     5341 tggatatttg gtattcgcat attatttaga caatcctttg atattgccct ttacagattt
     5401 tctatataca aattctgcgt tagcttttcc aatcttatta actttggtgg cagtcattgt
     5461 tataattatt ccatattact taggtttttt taagaatacg tttgaagggt ggtcgacaat
     5521 aagagtattt agactactag taattctatt tgttttattg ctccttatct attttactaa
     5581 tgtgttaatg attaggttag gtttttcatc ttcaaatcat gttggtagat cacaaaacta
     5641 tttgattacc atgccaaaag ttatgcaata ttatattatt ggaatagtag ctcctattat
     5701 tgaagaaatt ctttggagag tatttttatt taaagcagta ggaaataaaa atcttgccct
     5761 atttgtttca tggcctttat ttgcttttgc tcacacaggc ctttctcttg aaataattcc
     5821 ttatttgttt ttatccttca tcgttacttg ggcatattat cgcaatgaga atgtattaga
     5881 aagtatcttt attcatgctt cctataatct tttaagccct ttatctgctc aatttttaaa
     5941 ttcttttttt cattttatag tataggagaa gagaggttct gttgcgaagt tttaaaataa
     6001 aatacaacct ctctgaattt caattatctt aggctgatat tcccaataag attttgattt
     6061 cagtagatac cgaaaagcaa cgcttgctta tctaggttta gatgctgtaa caagagaaga
     6121 cttacttgat acgattaact ttgtataaca taaaatcccc cctttggtat taaaggaggg
     6181 atttttgtga actggtcaac caaaaaatat cattgttatt cctacaccaa taaagaagag
     6241 gtctaagatg actaaaagtg cgattgtaat gatttttccg aatttgttgt aagggttgat
     6301 acttataccg tatccaaata ctttgggtac ccagagtgga gagttgggga tgccctcata
     6361 atattgataa acaatcttac tgaatttaac agctgtatga attagcacaa tacataacaa
     6421 taaatcaaag agggtaaaag attttaatag tatcttgttg ttggacacta agggaatgag
     6481 tactactgag gtaagtacaa ttaacgcagt tagtataacc aataagacag aatatttctt
     6541 gttagctttc tttgtattaa ttagttcata atctttttgg acttggttca tgagttgtgc
     6601 atctcctttg ataatatcga gtgaaaaatc gtaatattta ctcaactcta caagcatggg
     6661 gatatctggg aaattcttcc cgttctccca atttgatagg gactgcctag taatgtatag
     6721 agtatctgcg acttcttgtt gagttatccc ttttttcttt cgttcttcta ctaaaatggt
     6781 tgataattcc ataattgttc ctttcttacc ccattatgat atattaaaag cgatttaatg
     6841 tctagcaaat atttcttgca tactggtata atgcggtaaa ggttatgaat gtaaactggg
     6901 ttgacacaga ctgtaaacag cttccttttc tctaaaagga gacttttctt tttgtcagat
     6961 ttgacctgta tcttttaaat tggtaagtag aagtaaagga aggaagttag ttcctcattc
     7021 tctcaatggg ttctgttgca aagttcccca taacacctga tttattgtaa aatgtaataa
     7081 aaaagaatgc gaggacaaca gatgaatcat tttaagggca aacaattcca aaaagacgtg
     7141 attattatct ctgttgggta ttatcttcgc tataatttga gttatcgtga tgttcaagag
     7201 atgttatatg atcgtggcat taacgtttct catacgacga tttatcgttg ggtgcaagaa
     7261 tatggcaaac tactctatca aaacggcttt tatcaaggga cagaacatcg taccattaaa
     7321 tacctgaata atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat
     7381 cgaagtttac gcactgcctc acccacgatt aaaggcatgg aagccatccg aggattatat
     7441 aagaaaaccc gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta
     7501 ttattgggaa tcccagatta aatcatagat accgtaaggg attttattct ttatttaaaa
     7561 ctttgcaaca gaacccttta gtcactctta ttttattttt tttgtttttt acaatactca
     7621 tataaattct ttaaaagtct taaaatgtaa gctatacctg tacatattaa gataatcaaa
     7681 ataaaattta aatgaatatt tgacaattga tttgcaatta aaccaagtat tattactact
     7741 aataatacta taaacatttc tttttctgat ttcaacgtct ttttactggt ccagctagac
     7801 gaccagcagt tgcccctttc acagctcctc cagcaatcgc tccccagatt gatccagatc
     7861 cattaatatt ttctagttct tcatccgtaa caatagcgaa ggtgcttttt gtctttctag
     7921 tttgaaaaat atactctatt tttttattcc tcgtaactca ttatatatac ttacaatcaa
     7981 ggctattact aataagcatt ccaatatttt actaaaaata tattgcccat aaaaaatact
     8041 gaatacagaa ataattaata gagggaattg ccataatagt gactgtatcc ttacattttt
     8101 tcttttctgt atcgaaatga ttgtagcaat taatcctaat ataaatacta ctagtataaa
     8161 aacattcata gggatagaga aataaaaaat cataaataat aatatataat agaaaacttc
     8221 tttatacttt gaataatttt ttttaataaa taatttcatt gttgcttaat caactttcaa
     8281 acttatttta tactattaaa tcataattca ttactaaaat aaactattag aattatggag
     8341 aacaattact tttaaatgca caagctattg tccagctacg aacccttatt aacctctaaa
     8401 ataatttagg ccaccagagt atgctctatt gaactatagc gaataaggaa aagaactctt
     8461 atttagaaga ggttctttct ttattcttat tttcttttaa attttgaatc gtcatttata
     8521 agtgaacgta tgaggtaata gatacctaaa aataaaaaga agaataacgg taatacgcgg
     8581 aataaagcaa agggagtttc aaaaagaaaa gcataccaaa aactgccacc ttttaaattc
     8641 cagatatttg gtgttagata ccatgacttg aagtttataa tttgggtcca ggatgtattt
     8701 ctactaaaaa tataaaaata caataaaaat aaaaatccgt atgaataacc atatttaaca
     8761 tatcttctct tcgcactttc ttgagaatct ttgtcagaaa atattcctct tgatacgtca
     8821 ggacgttttt gctgaaagta ctgagcacca ccagaacgac ttccttttat tagtttccaa
     8881 ccactatcag aaaatagtgt tacataatct tcatagtttg ttttcttcat acttttttct
     8941 ctaaaatcaa ggcgtacata aggaatgaac atttctttag tagataattt ttcaaaagta
     9001 tacataggga ctgcagaatt aacagaaact aacttatagc cctcttcttg tatagaatta
     9061 atccattctt cttctttttt taaagataag aaaattctaa atttcttcat gttttcccct
     9121 cctaatatcc aaattcagaa gatagtttaa tcagacttct taaacgttct atttctagtg
     9181 ttaaaacttc ttctcctata tcggtaattt tgtaaactct tcttcttttt tccctatttg
     9241 gaatttcttc tatccaatta agcttaagta aattttcaat cgctccatac atggttccag
     9301 ctgctacctt tacaaatcca ttactcaact tttctatttc ttgaataatg gcatatccat
     9361 gtgcaggagt ggttaaaact aatagaatgt aaaaagttgt ttcggttaaa ggtaaatgag
     9421 gatttctact catatatgtt cctccttttt ttattatata gttatactgt atatagtttc
     9481 tatgtataca gtataactat ataaatttag aaagtcaagt atcgttttgt gttcaacagg
     9541 gtataccctg atgtcctgtt tttaaattga tatatataaa aagtacctga gtaatcaaaa
     9601 gtggactatt atcaaataaa tagacataaa aaagaaaagc gttctaatcg tttttccttt
     9661 tttatcagat gttcagaaaa tggtcatttt ctgaacactc ttcttttcct attaaatttc
     9721 tcaaaatcat ttacatttaa tgttcattaa cccgtaattt attctatgtt catttatata
     9781 ggtgctttca agttatggta caatagtatc agcggaggaa taaaaatgaa tattggatat
     9841 gcccgagttt caactggact tcaaaattta gatttacaaa aagatagcct tgaaaaatat
     9901 aattgtgaaa aaatatttac tgaccatatg tcaggaagta aaaaagaaag acctggatta
     9961 aaatccgcag ttgaattttc ccgtcctggg gatacgattg ttgtttggcg attggatcga
    10021 ttaggtagaa atatggaaga tctcattaat attgttaatt ctcttaataa taaaggggta
    10081 agttttcata gtctacaaga aaatattaca atggataaat caagttcgac tggacaattg
    10141 atgtttcatt
//
