LOCUS       NC_013656              20858 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Lactococcus lactis subsp. lactis KF147, complete sequence.
ACCESSION   NC_013656
VERSION     NC_013656.1
KEYWORDS    RefSeq.
SOURCE      Lactococcus lactis subsp. lactis KF147
  ORGANISM  Lactococcus lactis subsp. lactis KF147
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 2598144)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B., Wels,M., van Hijum,S.A.,
            Molenaar,D. and van Hylckama Vlieg,J.E.
  TITLE     Complete genome sequence of Lactococcus lactis subsp. lactis KF147,
            a plant-associated lactic acid bacterium
  JOURNAL   J Bacteriol 192 (10), 2649-2650 (2010)
   PUBMED   20348266
REFERENCE   2  (bases 1 to 2598144)
  AUTHORS   Siezen,R.J., Starrenburg,M.J., Boekhorst,J., Renckens,B.,
            Molenaar,D. and van Hylckama Vlieg,J.E.
  TITLE     Genome-scale genotype-phenotype matching of two Lactococcus lactis
            isolates from plants identifies mechanisms of adaptation to the
            plant niche
  JOURNAL   Appl Environ Microbiol 74 (2), 424-436 (2008)
   PUBMED   18039825
REFERENCE   3  (bases 1 to 2598144)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B.A.M., Wels,M., van
            Hijum,S.A.F.T., Molenaar,D. and van Hylckama Vlieg,J.E.T.
  TITLE     Complete genome sequence of Lactococcus lactis ssp lactis KF147
  JOURNAL   Unpublished
REFERENCE   4  (bases 1 to 2598144)
  AUTHORS   Siezen,R.J., Bayjanov,J., Renckens,B.A.M., Wels,M., van
            Hijum,S.A.F.T., Molenaar,D. and van Hylckama Vlieg,J.E.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-DEC-2009) NIZO Food Research, Kluyver Centre for
            Genomics of Industrial Fermentation, PO Box 20, Ede 6710 BA, The
            Netherlands
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000025045.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 01:08:29
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,639
            CDSs (total)                      :: 2,548
            Genes (coding)                    :: 2,493
            CDSs (with protein)               :: 2,493
            Genes (RNA)                       :: 91
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 55
            CDSs (without protein)            :: 55
            Pseudo Genes (ambiguous residues) :: 0 of 55
            Pseudo Genes (frameshifted)       :: 25 of 55
            Pseudo Genes (incomplete)         :: 23 of 55
            Pseudo Genes (internal stop)      :: 14 of 55
            Pseudo Genes (multiple problems)  :: 5 of 55
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 08:59:28
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 896046
            Orig. end                         :: 916904
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP001834.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            Bacteria source: Dr. W.J. Kelly, Agresearch Limited, New Zealand;
            william.kelly@agresearch.co.nz.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20858
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="3"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20858
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20858
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10858](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10858
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     gene            131..640
                     /locus_tag="LLKF_RS04425"
                     /old_locus_tag="LLKF_0864"
     CDS             131..640
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LLKF_RS04425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LLKF_0864"
                     /product="hypothetical protein"
                     /protein_id="WP_012897501.1"
                     /transl_table=11
                     /translation="MRGKIKLNIFPSVNRLVAKEIIKYIDWLKERYSFPEQLTIIITGA
                     SYLVSDKTGNHVTSTFGKPWGEQKDYYMKIAVGDFFEMHRWGRRNAVYSILNDISYGLM
                     QYYDLLSTEKADFNEDEIDKKATQLTIDYSIERGVLLGDMYRKIHHWGMSAVRKYRNSK
                     SNRKKK"
     gene            651..1148
                     /locus_tag="LLKF_RS04430"
                     /old_locus_tag="LLKF_0865"
     CDS             651..1148
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LLKF_RS04430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LLKF_0865"
                     /product="hypothetical protein"
                     /protein_id="WP_012897502.1"
                     /transl_table=11
                     /translation="MFQEIYTKDNEENFYVGKKLGRKENFYLYELIDDKGRFLSISLIH
                     KNIINKTIDSSEYLDLMNSFVKTNDKFFDIFNLSRDFEAKKFNFSHLLNKNILYVRGKK
                     DLIEISKVVKVKEESLFLGKIDSPLYDSIGEELSEVLFSDFEKVDIVSVEEYLMRQYLK
                     KM"
     gene            1171..1710
                     /locus_tag="LLKF_RS04435"
                     /old_locus_tag="LLKF_0866"
     CDS             1171..1710
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012897500.1"
                     /locus_tag="LLKF_RS04435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0866"
                     /product="hypothetical protein"
                     /protein_id="WP_012897503.1"
                     /transl_table=11
                     /translation="MFELKLWIKPTNTKWLVKEISQYLDWIIKKGIFDGEINIFLTNAK
                     FVYDSSARKREGNFFGPFDKSIIPSLYFSLGDIFRTISCRGKENAVCDWLQYLTLFLYD
                     YVDWQEDREFNSELNNDLADKMIYEYIDFKKISFESQDRRKREKRKRAKIRVRRKKKKK
                     THEKNGKGVSKKINEG"
     gene            complement(1775..2932)
                     /locus_tag="LLKF_RS04440"
                     /old_locus_tag="LLKF_0867"
                     /pseudo=""
     CDS             complement(1775..2932)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1164: transposase
                     IS3/IS911 family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611914.1"
                     /locus_tag="LLKF_RS04440"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0867"
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSGFKRYDEEFKQSLVNLYQTGKTQSELCKDYGVSVSALAKWIKQ
                     YSQVRLEDNTVLTAKQIQELQKRNAQLEEENLILKKASAIFMQNSK"
     gene            3101..3472
                     /locus_tag="LLKF_RS04450"
                     /old_locus_tag="LLKF_0869"
     CDS             3101..3472
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LLKF_RS04450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LLKF_0869"
                     /product="hypothetical protein"
                     /protein_id="WP_012897504.1"
                     /transl_table=11
                     /translation="MDNLKKMQGPGKRWNEEQKNEVHYERNGLFRFESLTKQKPRKIKR
                     GKSYQVFMENIDIYLDSNEVRLEIKKLLQDTKDYLLQGVFDVFDANRYLRGNIYFLVHQ
                     GSQLNDYERKILKSFKNKP"
     gene            complement(3587..5665)
                     /locus_tag="LLKF_RS04455"
                     /old_locus_tag="LLKF_0870"
     CDS             complement(3587..5665)
                     /EC_number="7.2.2.-"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0015662 - P-type ion transporter activity
                     [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1037: heavy metal
                     translocating P-type ATPase"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905590.1"
                     /locus_tag="LLKF_RS04455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0870"
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="WP_012897505.1"
                     /transl_table=11
                     /translation="MSNKTHSSNHDEMMSEHHHNHEIMDMEHENHEMHNMDNMHDMHNM
                     DMMNHGGHMMHMGDMSKKLKVAIILMIPLLLISPIAGFTILKFPGSEILQLILGTIIFF
                     YSGTPFFSGAKGELKSRKPAMMMLITMGITVAYAYSVYATIMSLNGYMGMNFWFELATL
                     IVIMLIGHLIEMKAIMGAGDALKDLASLVPKKAHLKSGKDVELSELKVGDLLLVKENEK
                     IPADGLILSEALVDESMITGESRAVNKKTNDLVYGGSLNQNQPFEMKVTTLGKDSFLNQ
                     VAELVKKAQAQKSNLENMADRVAGYLFYAALIVGIFSLVFWTISSNFSFALLLAVSVFV
                     IACPHALGLAVPLVVSRLTSISAKNGLLIQNRTSLEKINTIKYALMDKTGTLTDGKFIV
                     RNVIDFTDETDILQIMAALEGSSTHPIAQSIVSAAKPLENLKVEAVENIPGVGIKGQVN
                     QNFYQIVNYKYLRENQLSYDEKKIAQYLDLGLTVSFLINEQQDVLGFIALGDSPKSDAK
                     AFINGLLAQGITPVMLTGDNKETAQKIASALNIPEFRAELKPEDKAEIVKEYQKKAGVL
                     FIGDGVNDSPALATATIGFAIGAGTSVAISTADVVLVNSNPSDVLDMINISKRMLRKMK
                     QNLWFGAGYNIIAIPVAAGILYPFTGIYIDPLIAAVLMSISTVIVSINAMGLRYDKK"
     misc_feature    complement(5710..5725)
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: strong, score: 30.52"
                     /tool="antismash"
     gene            5811..6128
                     /locus_tag="LLKF_RS04460"
                     /old_locus_tag="LLKF_0871"
     CDS             5811..6128
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="LLKF_RS04460"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="LLKF_0871"
                     /product="hypothetical protein"
                     /protein_id="WP_012897506.1"
                     /transl_table=11
                     /translation="MLDSCYAILASAESDGSTTSSSMLTNPNLSSNEAKVSTLISKVSS
                     SPSLSNSLSAKSLQLVPTVTKEELLKTDADDDLSLVLAELGLGLVATLTIGKVKYKKDF
                     E"
     gene            6284..7138
                     /locus_tag="LLKF_RS04465"
                     /old_locus_tag="LLKF_0872"
     CDS             6284..7138
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131344.1"
                     /locus_tag="LLKF_RS04465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0872"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="WP_012897507.1"
                     /transl_table=11
                     /translation="MNLIDGKALAAKMQAELKVKVDKLKEADNVPGLAVILVGEDPASQ
                     IYVRNKARQATAIGLNSSVVRLPETVSEQELLDLIEQYNQSEQWHGILVQLPLPEHISE
                     EKVLLAIDPEKDVDGFHPMNMGRLWSGNPLMIPSTPAGIMEMFREYNVELSGKRAVVIG
                     RSNIVGKPMAQLLMMADATVTIAHSKTENLRELTKEADVLVVAIGRDRMIKAEDVKEGA
                     VVIDVGMNRDEDGKLHGDVDFDEVKDVASLITPVPGGVGPMTITMLMEQTVRAASRKIN
                     ENK"
     gene            7305..8558
                     /gene="xseA"
                     /locus_tag="LLKF_RS04470"
                     /old_locus_tag="LLKF_0873"
     CDS             7305..8558
                     /EC_number="3.1.11.6"
                     /GO_component="GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA]"
                     /GO_function="GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /GO_process="GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="xseA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131345.1"
                     /locus_tag="LLKF_RS04470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0873"
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="WP_012897508.1"
                     /transl_table=11
                     /translation="MTEYLSVSTLTKYLKAKFDRDPYLERVYLTGEISNFRRRPNHQYF
                     ALKDEGAVIQATMWAGQFRKLDFELEEGMKVLAVGRISIYPPSGSYSINIESLVPDGVG
                     ALAVKFEQLKKKLTAEGLFEQRWKQTLPQFSKKIAVVTSPSGAVIRDIITTVQRRFPMS
                     QIVLYPTKVQGQGSAEEIAGNIRRANQRGDFDVMIIGRGGGSIEDLWGFNEEIVVRAIF
                     ESRIPIISSVGHETDVTLADFVADSRAATPTAAAELATPNTKVDLINWTNEQEKRLFNR
                     LTHLIKIRRERVDKLSQSVVFRQPERLYDGHLEKLDRLCERLSVLTENKVANMKHRYEL
                     SAGKLIPTYGKIVEAKKNKTEQLYQSLLLLDISKIKARGFSLVTDEKGKIIKSVSDVKK
                     GQTLDVELTDGKVIVEVK"
     gene            8558..8797
                     /locus_tag="LLKF_RS04475"
                     /old_locus_tag="LLKF_0874"
     CDS             8558..8797
                     /EC_number="3.1.11.6"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131346.1"
                     /locus_tag="LLKF_RS04475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0874"
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="WP_003131346.1"
                     /transl_table=11
                     /translation="MATKKEDVKFEDNLAELENIVRKLESGDVALEDAIAEFQKGMKIS
                     ETLKKTLNEAEETLVKIVGKNDQESDFSPEQKDY"
     gene            8947..9919
                     /locus_tag="LLKF_RS04480"
                     /old_locus_tag="LLKF_0875"
                     /pseudo=""
     CDS             8947..9919
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017864361.1"
                     /locus_tag="LLKF_RS04480"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0875"
                     /product="glycerate kinase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MKKKFVLAPDSFKESLSALEVCEAMEKGIKKVFPKAEIISMPMAD
                     GGEGTTDALIDATGGRRVYKKVQGSFANTNVEAYFGILGNSNTAVIEIAKANGLELVAL
                     EDRNPLITSTYGTGQLIKAAMDEGVDKIIIGIGGSATNDGGSGMARALGAKFLNKDQEE
                     IPLGGGFLKEVRTIDMSDFDPRVEEIEILIATDVTNPLTGNNGASYVFGPQKGATEEMV
                     KVLDENLKHYAEIVHNDLGKDVKNKPGAGAAGGLGAGLLCFGRIYWGWSRCSL"
     gene            10001..10858
                     /locus_tag="LLKF_RS04485"
                     /old_locus_tag="LLKF_0877"
     CDS             10001..10858
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012897509.1"
                     /locus_tag="LLKF_RS04485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0877"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_012897509.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 2.7e-53, bitscore:
                     178.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDTKILKLEDFLTEFYESAEFPTGLAESAKYSLLAGGKRIRPLLF
                     LNLLEAFDLELSKAHYHVAAALEMIHTGSLIHDDLPAMDNDDYRRGQLTNHKKFDEATA
                     ILAGDTLFFDPFFILSTADLSAEIIVALTRELAFASGSYGMVAGQILDMAGEGKELTLA
                     EIEQIHRLKTGRLLTFPFVAAGIVAQKSTDEVEKLRQVGQILGLAFQIRDDILDVTATF
                     AELGKTPGKDILEEKSTYVAHLGLEEAKKSLTGNLSEVKKLLTDLSVTDSSEIFKIIEQ
                     LEVK"
     misc_feature    10911..10924
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     medium, score: 24.74"
                     /tool="antismash"
     gene            10937..11743
                     /locus_tag="LLKF_RS04490"
                     /old_locus_tag="LLKF_0878"
     CDS             10937..11743
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131349.1"
                     /locus_tag="LLKF_RS04490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0878"
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="WP_012897510.1"
                     /transl_table=11
                     /translation="MKERIDVLATNQGLFETREQAKRGVMAGLVVDSKSGERYDKPGQK
                     IDDGTELRLKGEKLRYVSRGGLKLEKALKEFHLEINEKTCLDIGSSTGGFTDVMLQNGA
                     KLVYALDVGTNQLAWKIRSDERVVVMEQFNFRNAVLADFEQGRPSFTSIDVSFISLDLI
                     LPPLYEILEENGEVAALIKPQFEAGREQVGKNGIIRDPKVHQMTIEKVLKTATQLGFSV
                     KGLTFSPIKGGAGNVEFLVHLLKDGKAEIAQQVNIESVLQKESEEL"
     gene            11740..12186
                     /locus_tag="LLKF_RS04495"
                     /old_locus_tag="LLKF_0879"
     CDS             11740..12186
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015426160.1"
                     /locus_tag="LLKF_RS04495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0879"
                     /product="arginine repressor"
                     /protein_id="WP_003131351.1"
                     /transl_table=11
                     /translation="MKREDRLNFIAQFIRENEIKTQEELVNTLLTHGIDVTQATVSRDI
                     KSLALIKVPAESGGYRYDLPKNKEVLQSSLHKALAFDAITGTKIKDNMLWILANPGTTS
                     LVKNYLLEEYSDDIFSIIIDDDSALVIFEMEEDAKNLYNLLTEF"
     gene            12317..14011
                     /gene="recN"
                     /locus_tag="LLKF_RS04500"
                     /old_locus_tag="LLKF_0880"
     CDS             12317..14011
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006281 - DNA repair [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /gene="recN"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923612.1"
                     /locus_tag="LLKF_RS04500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0880"
                     /product="DNA repair protein RecN"
                     /protein_id="WP_012897511.1"
                     /transl_table=11
                     /translation="MLQEISIKNFAIIEEIHLSFESGMTILTGETGAGKSIIIDAMSLL
                     LGGRASSDFVRHGANKAEIEGLFFFDKTPELKAVLLELGFEEVDSEIILRREIFANGRS
                     VCRINGQMVNLATLRQVGELLVDIHGQHDSQELMNPKSHLRLLDEFGDEAFDQIKNNYK
                     LKFENFKNLRQQLNLRQKNEQEFAQRIEILQFQAEEIEAAEINLEEDEQLVNRREKLNN
                     IKNIADSLSSAYLALDDEDNDYSSLNNIRTTMTELDKISKFDNDYQELADKTAESYYVL
                     EEVANQIQRIMSDLEFNPAELLQIEDRIMTLTTLKKKYGPELSDVMNYLEKVQLELSEL
                     TGSENDSENLVNTVKVAQVQLIEASKKLNEARHKIAKEVEKDIKHELSELYMEKADFKV
                     SFEGAKFSANGNEHVEFFIQTNPGEGFKPLAKTASGGELSRLMLAIKSSFSRRENKTAI
                     VFDEVDTGVSGRVAQAIANKIYKIARHGQVLAISHLPQVVAIADRQFYIEKASTDDITT
                     STVRKLNAEERVNEIAKMLAGEDLTKEALAQAKRLLSNKSNIIKKKS"
     gene            14709..15275
                     /locus_tag="LLKF_RS04510"
                     /old_locus_tag="LLKF_0882"
     CDS             14709..15275
                     /GO_process="GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011675826.1"
                     /locus_tag="LLKF_RS04510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0882"
                     /product="TMEM175 family protein"
                     /protein_id="WP_003131353.1"
                     /transl_table=11
                     /translation="MSKTRLEAFTDAVIAIIMTVLVLELVRPDSDSLQSFIALLPHLGV
                     YFVSFFILAIYWVNHHHLFQSIKRINGKVLWINISFLFVLSLIPIFSNWVSIYPNSFIP
                     ELGYVIIFFFGNFIYLLLTRELLKINGHRKTSETTIRKNILSVVLNIVSILLGYFIAPV
                     IMLVGSALIFSMWVIPDKNIEKMFK"
     gene            15373..15894
                     /locus_tag="LLKF_RS04515"
                     /old_locus_tag="LLKF_0883"
     CDS             15373..15894
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015426163.1"
                     /locus_tag="LLKF_RS04515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0883"
                     /product="DUF805 domain-containing protein"
                     /protein_id="WP_012897512.1"
                     /transl_table=11
                     /translation="MIKAYIKYWKKAGDLKSYSNRSDYWWVFLANSIIFAILSILNFMV
                     TIPKAGKIMSQSATLSQKEIIQQVTDLYANPTGGALVIVIVTALIGLAILVPNISLTAR
                     RLRDARFPWWIALVFGVAAIYGLVTMFIHQDLLAKMGYFVGFINFIIYILCLFPTKYGI
                     DEEDDSRTYE"
     gene            15918..16856
                     /locus_tag="LLKF_RS04520"
                     /old_locus_tag="LLKF_0884"
     CDS             15918..16856
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017864363.1"
                     /locus_tag="LLKF_RS04520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0884"
                     /product="DUF805 domain-containing protein"
                     /protein_id="WP_012897513.1"
                     /transl_table=11
                     /translation="MVQAYKKFWLGAFTFNRKTSRKDFWSALLTHIIIFVILFKAYHFF
                     NLLDFYQLTTLWQTFASFFQLIFNLYFFGSLLSFIALTVRRLNDADLPWALIFLNFILG
                     LGTLVLLVLNLFPSSPRALKFKEYEINSSQEFNNLPETKTLSGIFKDYFKNYFEFRGRT
                     TRRNFWWMQLFWGLTFILFLFLIYLFNQFEQIMFGYNFIGSMVLRLLFFLFILGTFFPQ
                     LTIHVRRLRDAGLSNLGLSLLLGGTSGILIFYQMFTKTLKITYTTGHYQLVQYLLFLLV
                     MIAVLSLILVEVMATGELKTNKKNSLFEKID"
     gene            17076..18029
                     /gene="rsmH"
                     /locus_tag="LLKF_RS04525"
                     /old_locus_tag="LLKF_0885"
     CDS             17076..18029
                     /EC_number="2.1.1.199"
                     /GO_function="GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="rsmH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017864364.1"
                     /locus_tag="LLKF_RS04525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0885"
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="WP_012897514.1"
                     /transl_table=11
                     /translation="MPKTIAFKHDTVLLHETVDMLEVKPNGIYVDATLGGAGHSEYLLS
                     KLTDGHLYSFDQDETAHENAKVRLSEQLAEDKVTLIKSNFRYLKSALAELGVTKIDGIL
                     YDLGVSSPQFDDSQRGFSYKKEARLDMRMDQNQTLSAYEVVNEYPYEALVRIFFRYGED
                     KFSKQIARKIEQARKIKPIETTIELADLIKSALPQKELKKKGHPAKRIFQAIRIEVNDE
                     LGAAEESIEQAIDLLKVDGRISVITFHSLEDRLTKTIFKEYSTVNVPKGLPMLPKDMEA
                     KLKLINRKPVLASEEELEFNNRAHSAKLRVAQKQRD"
     gene            18187..18567
                     /locus_tag="LLKF_RS04530"
                     /old_locus_tag="LLKF_0886"
     CDS             18187..18567
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003131357.1"
                     /locus_tag="LLKF_RS04530"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0886"
                     /product="cell division protein FtsL"
                     /protein_id="WP_003131357.1"
                     /transl_table=11
                     /translation="MAAQPKEYFDIETAAKKDSYLIDTETLAPEVLASKFKKFSGVEKV
                     FYLTMVVAALVLAVGLLYVKTKTLEVQGNTMDLRSKISQETTRKTEYDQQILDLTSGTK
                     VLDTANKADLKQNPSNVLKATK"
     gene            18564..20855
                     /locus_tag="LLKF_RS04535"
                     /old_locus_tag="LLKF_0887"
     CDS             18564..20855
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /GO_process="GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010905598.1"
                     /locus_tag="LLKF_RS04535"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="LLKF_0887"
                     /product="penicillin-binding protein"
                     /protein_id="WP_012897515.1"
                     /transl_table=11
                     /translation="MIKFLKKLIFFPFRKVSRNARKTNWSTERNRKRVGKSLFFLAIAL
                     FTVFIFRFVWLITVNHVGGTNLTTMAKSNYQSTVTVQAKRGTIYDRTGAAIAVDSSTYT
                     IYAVIDKTQVDSNGNPLYIDKKDFTKVEDFLNSKLGIDRDLIKKQLNSKLKQVQFGPKG
                     SDISLEKMKEIQKAAENEKIVGLGFTANISRSYPFGNFASQFIGIARPKDENGTQALKG
                     DMGLEKAFNNVLSGENGKETYQKDIYGRPIPGTTKVIEPVKNGQDVYTTLDAQLQRNLE
                     GYMDKAATDTGAQQLSGTLVDAHTGEILATSQRPTYTATTINDAEKQKYFTWNSLLSQS
                     AFEPGSTFKTFLMAGALDSGKVNLNETYQRKLQVYDTTINDWDVTENKSYTLPETVTYA
                     QGFALSSNIGMSKIEMNMGNDLWGSYLNKFKFGLKVRAGLDGENPGALPSSNAVTQIQS
                     SFGQGVAVTPLQLIRGWTAIAGNGTMLEPHIVSKVVDPNTKTSLTSKAEVVGHPVSNDA
                     ASGVRDLMLTVNTDPVYGTSYSTSGDSEQNLAAGPLFMVNGEPAAVKTGTAQIASTTGG
                     YMTGSQDYLYSAVVMYPAKNPDFIFYMNVKIPTESWTLKYIARVANPLLTSAEAMKNDL
                     SVSADTDTKAGKVTIENYKGKDSGDTADSLRRTVLSPVIIGTGAKVTAQSIAEGEKVSA
                     NSRILLLTNDKDQVMPDMYDWSKKEVEQLANWFGIKVTYEGSGNKVLTQSIETSTNVKK
                     GQTLTVKMGN"
ORIGIN
        1 aaaattagct ttgaatcaga ggatagaaga aaaaaggaaa atcgaaaacg agcaaaaaat
       61 aaaggtaaga agaaagaaaa aaagagaaga caaaacaaaa gaatttgaaa gaaagactga
      121 ataaaagaga atgaggggaa aaattaaact aaatattttt ccttccgtta atagattagt
      181 tgcaaaggag attataaaat atattgattg gctaaaagaa agatattcat ttccagagca
      241 attgacaatt attattactg gggcttctta tcttgttagt gataaaactg gaaatcatgt
      301 gacttctaca ttcggaaagc catggggaga gcaaaaagat tattatatga aaatagcggt
      361 aggagatttt tttgagatgc atcgctgggg gagaagaaat gctgtttata gtattttaaa
      421 tgatatatcg tatggtctta tgcaatacta tgatttactt agtacagaaa aagccgattt
      481 taatgaagat gaaatagata aaaaggctac ccaactaaca atcgactatt ctattgaacg
      541 aggcgttttg ctaggggata tgtacagaaa aatacatcat tggggaatgt cggcagttag
      601 aaaatataga aatagtaaat caaatagaaa aaagaagtga gaaataggta atgtttcaag
      661 aaatttatac aaaagataat gaagagaatt tttatgttgg taaaaagctt ggaaggaaag
      721 agaattttta tctttatgaa ttgatagatg ataaaggacg ttttctttct atatctttaa
      781 tacataaaaa tattattaat aagacaatag attcaagcga gtatctggac ttaatgaatt
      841 cttttgtgaa aactaatgac aaattttttg atattttcaa tctttcaaga gattttgaag
      901 caaaaaaatt caatttttct cacctgctta ataaaaatat cctttatgta cgtgggaaaa
      961 aagacttgat agaaatatca aaagttgtaa aagttaaaga agagagtcta tttttaggaa
     1021 aaatagactc cccattatat gactctattg gtgaagaact ttctgaggtt ttgtttagtg
     1081 attttgagaa agttgatata gtctcagttg aagaatactt aatgaggcag tatttgaaaa
     1141 aaatgtaaat atttaggtgg aaaataaaag atgtttgagc taaaactatg gattaaacca
     1201 acaaatacaa agtggcttgt taaagaaata tctcaatatt tggattggat aataaaaaaa
     1261 ggaatttttg atggagaaat aaatatcttt cttacaaatg ctaaatttgt ttacgattct
     1321 agtgctagaa aaagagaagg aaactttttc ggaccttttg ataaatcaat tattccgtca
     1381 ctttattttt cgcttggaga tatttttagg acaatatctt gtagaggaaa agaaaatgcg
     1441 gtctgtgatt ggctacaata tttaacgctc tttctctatg attatgtaga ttggcaagaa
     1501 gacagggagt tcaatagtga attaaacaat gatttagcag ataaaatgat ttatgaatat
     1561 atagatttta aaaaaatcag ctttgaatca caggatagga gaaaaagaga aaaaagaaag
     1621 cgggcaaaaa taagggtaag aagaaagaaa aaaaagaaga cacatgaaaa gaatgggaaa
     1681 ggagtttcta aaaaaataaa tgaaggatag aaaaaatgag aaaattgcgt acaatgatag
     1741 cctagaaaat ttagactgga ctaatgtcaa atatttagac acaaaatgag ctatcttttc
     1801 ttaaaatagt tctcttcctt ctgatttgga gttaatccat tgttggctga atgaggatta
     1861 tagttgttat aaaattgttc gatgtattca aaacaagaga gttgcacttc ctgaatggag
     1921 tgataagttc ttctgtttat ttccctttgt ttaaggtact tgaaaaacac ctcagtcacg
     1981 gcattatcat aaggataacc aggtttggaa taagaagcaa gcaactgatg gtcatctaat
     2041 attttcctaa aagaagctga tttaaattgg cttccttgat cggaatgaaa aacaattggt
     2101 tccttaggct tcctcttatt tatagccatc tctaatgtgt cacaggcaag tttagcatca
     2161 atcttatcac ttactttcca agcgatacat ttcctagaat agaggtcaag aatagcgcag
     2221 agataaacgt gacgcttagg atcgatagaa atataagtaa agtcggttgt ccacacttga
     2281 tttgggactt taggattaaa ttcttgttta agcaagttat cagaagaaaa cataggagct
     2341 ttctttgatt taaaccgagg tttgagggtt gacattttag ggagtgtcat agacttgaga
     2401 agtcgtaaga tacgcccttc agaaatatta acgccataat cacgcagaag aatgatttta
     2461 aaagctctcg ttccaattct tttcttagct ttcatataaa tctcaaggag taattttctc
     2521 aagcgttgat tttctatttc acgctttgag ggcttcttgt ttatgaagtt atagtaggtg
     2581 gaacgattga catgtaaaac acgacagagc ataactgtcg tgtgttcaat tcggagtcta
     2641 tagactgctt ttaatctcac ttggagtttt gcatgaatat ggcactcgct ttttttaaga
     2701 taagattttc ttcttcgagt tgggcattcc ttttttgtaa ttcttgaatc tgtttggcag
     2761 tcaacaccgt attatcttca agacgaactt gagagtactg cttaatccat tttgcaagag
     2821 cagagacgga gacgccatag tctttacaga gttcggattg agttttacct gtttgataga
     2881 ggttgacgag agattgttta aattcctcgt cgtaacgttt aaagcctgac ataaaagtcc
     2941 tttcgttttt gtgtcttctt agacagatta taacacacta agttgtgtct acttttatag
     3001 tatagctcca gacgatatat ttttaatcaa agataaaaat aaacctaaag caaataaaga
     3061 aaggagcagt gaagaaaaag caagggaaac caagcaaatt gtggataatc ttaaaaagat
     3121 gcaaggtcct ggaaagagat ggaacgaaga acaaaaaaat gaagttcact atgaaagaaa
     3181 tggactcttt agatttgaaa gccttactaa acaaaagcca agaaaaatta aaagaggaaa
     3241 aagctatcaa gtgtttatgg aaaatataga tatttatctt gattctaatg aggttagatt
     3301 agaaataaaa aaactgcttc aagatactaa agattattta cttcaaggag tctttgatgt
     3361 ttttgatgct aatcgttatc ttagaggtaa tatttatttt ctagttcatc aagggagtca
     3421 attaaatgat tatgaacgaa agattctaaa atcatttaaa aataaaccat aaaaaatatc
     3481 attacaatca ttagtatttg tgtgatatca tacttaatct ttaagtatat ttaagtgata
     3541 aaagtttcat aattagtatg aaaaaaatag aagagcatgt gctcttctat tttttatcat
     3601 acctcaatcc catcgcgttg attgagacaa tcacggttga gattgacatc aggactgctg
     3661 caatgagagg gtcaatataa atccctgtaa atggataaag aatacccgct gcaacaggaa
     3721 tagcaataat gttatagcct gctccaaacc agaggttttg tttcattttt ctaagcatgc
     3781 gtttggagat attaatcata tccaagacgt cacttggatt ggagttaact aggaccacat
     3841 cagcggtact gatagcaacc gaagttcctg ctccaatcgc aaaaccaatc gtagcagttg
     3901 caagagctgg ggaatcattg acaccatcac caatgaaaag cacgccggct ttcttttggt
     3961 actctttcac aatttctgct ttatcttcag gttttaattc cgctctaaat tcgggaatat
     4021 ttagtgcact agcaattttt tgagcagttt ctttattatc acccgtgagc ataacagggg
     4081 taattccttg ggctaataaa ccattaataa aggctttcgc gtctgattta ggagaatctc
     4141 ctaaggcaat aaatcctaaa acatcttgtt gttcattgat taggaaagat acagttaaac
     4201 ctaaatctaa atattgagca atcttttttt catcataaga aagttgattt tcacgaagat
     4261 atttatagtt aacaatttga taaaagtttt gattaacttg ccctttaatc ccaactcctg
     4321 gaatattttc aaccgcttca acttttagat tttcaagagg tttagcagct gaaacgattg
     4381 attgagcaat cgggtgggtg gaacttcctt caagtgctgc catgatttgg agaatgtctg
     4441 tttcatcagt aaaatcaata acattacgaa caataaactt accgtcagtt aatgttcccg
     4501 ttttatccat taaggcatat ttaattgtat ttattttttc tagagaagtt ctgttttgaa
     4561 ttaataaacc atttttagca gaaatactgg ttaggcgaga aacaacaagt ggaaccgcaa
     4621 gacctaaagc gtgtggacat gcaattacaa aaacagaaac cgcaagaagt agagcaaaac
     4681 taaagttaga actaattgtc caaaaaacta atgagaagat accaacaata agtgcggcat
     4741 agaaaaggta accagccact ctatctgcca tattttccaa attagatttt tgagcttggg
     4801 ctttcttgac taattcggcc acttgattga gaaatgaatc ttttccaagg gttgtgactt
     4861 tcatctcaaa tggttggttt tgattgaggg aaccaccata aacaaggtca tttgtttttt
     4921 tattgactgc tcggctttca ccagtaatca ttgactcatc aacgagcgct tcacttaata
     4981 taagaccatc tgcaggaatt ttttcatttt ctttcactaa aagcaaatca ccaactttta
     5041 gttctgaaag ctccacatcc ttcccacttt ttaagtgagc ttttttggga acaaggcttg
     5101 ctaaatcttt caacgcatcg ccagcaccca tgattgcttt catctcaatc agatgaccaa
     5161 tcagcataat gacaattaat gttgccagtt caaaccagaa gttcatgccc atatatccgt
     5221 taagactcat gattgtggca taaacagaat aagcataagc aacggtaatt cccattgtaa
     5281 tcagcatcat catggctggc ttacggcttt ttaattcacc ttttgctccg ctaaaaaaag
     5341 gagttccaga gtaaaagaag ataattgtac caaggatgag ttgcaaaatt tcacttccgg
     5401 ggaatttaag gatagtaaag cccgcgattg gtgaaatgag tagaagcgga atcataagaa
     5461 tgatagccac tttgagcttt ttactcatat cacccatatg catcatgtgt cctccatgat
     5521 tcatcatatc catattgtgc atatcatgca tattatccat attgtgcatc tcatggtttt
     5581 cgtgctccat atccattatt tcatgattat gatgatgctc agacatcatt tcatcgtgat
     5641 tagacgaatg agttttattt gacatatttt cctcctaaat gaatgctttg aattttctat
     5701 ctatatatag tttacaattg taaactatta aagtcaagga aaaggtaaca gcaaagagat
     5761 actaagaatt caagttttag tatgagtgga gtgataaaag caattcagat ttgctagatt
     5821 catgctatgc tattttggct tccgctgagt cagatggttc aacaacatct tcaagtatgt
     5881 tgactaatcc aaatttatct tcaaatgaag caaaagtttc gaccttaatt tctaaggtat
     5941 caagtagtcc gagtctttca aattcattaa gcgcaaagag cttgcaactt gtaccaactg
     6001 taacgaaaga agagcttctc aaaacagatg ctgatgatga tttaagttta gtattagcag
     6061 aattgggatt gggcttagtg gcaacgttaa ctattggaaa agttaaatat aaaaaagatt
     6121 ttgagtagta tattttaatt aaggacttct tttctttgga aagaagtttc tttttaattt
     6181 ggaattattc ctttattaaa aattacgaac aatttaaaat aaagaaagaa aaaggaatga
     6241 tgatgattag aggatgaatg ttagcttatg gtataatagt tctatgaact tgatagatgg
     6301 aaaagcactt gctgctaaaa tgcaagctga gttgaaagtt aaagtcgata aattaaaaga
     6361 agcggacaat gttccaggtc ttgccgttat tcttgtgggc gaagatcctg cgagtcaaat
     6421 ttatgtccga aataaagcac gacaagccac agcaattggt ttaaattcta gtgtggttcg
     6481 tctaccagaa actgtgtcgg aacaagaact gttagattta attgaacagt ataatcagtc
     6541 agaacaatgg catggtattt tggtacaatt gccattgcca gaacatattt ctgaagaaaa
     6601 ggttttacta gctattgatc cagaaaaaga tgtggatggt tttcacccaa tgaatatggg
     6661 acgtttgtgg tctggaaatc ctttgatgat tccttcaact ccagccggta ttatggaaat
     6721 gttccgtgaa tataatgtgg aactttctgg taaacgtgca gttgtgattg gtcgttcaaa
     6781 tatcgtcgga aaaccaatgg cgcaattatt aatgatggct gatgctactg tgacgattgc
     6841 tcattcaaaa acagagaact tgcgcgaatt gactaaggaa gctgatgttt tggtggttgc
     6901 tattggccgt gaccggatga ttaaagctga ggatgtcaaa gaaggtgcag ttgtaattga
     6961 tgttgggatg aaccgtgatg aagatggaaa acttcatggc gatgttgatt ttgatgaagt
     7021 aaaagatgtt gcaagtttga ttacccccgt tcctggtgga gttggtccga tgacgattac
     7081 aatgcttatg gaacagacag ttcgtgcagc aagtagaaaa ataaatgaaa ataaatagtt
     7141 aattaataaa atagtaacta agtttatagt aatttctaag gacttatttt taagttagaa
     7201 gttttttgaa ggtaaacttt ttactttttt tcgattttag aagtagaaat tttactaaca
     7261 gaatgattag taaaaagatt aattgagaac taaagagtga gggaatgaca gaatatttat
     7321 cagtatcaac cttaacaaaa tatttaaaag cgaaatttga ccgtgatcca tatcttgaac
     7381 gggtttattt gacaggagaa atttcaaatt ttagaagaag accaaatcat caatattttg
     7441 cacttaaaga tgagggcgct gttattcaag ctacgatgtg ggcgggacaa tttcgcaaat
     7501 tagactttga attagaagaa gggatgaaag ttttggctgt tggtcgaatc agtatttatc
     7561 caccttctgg ctcttattct ataaatattg aaagtttagt tccagatggc gttggagctc
     7621 ttgcggttaa gtttgaacag ttgaagaaaa agctgactgc tgaagggcta tttgaacaac
     7681 gttggaaaca aactttgcca caattttcta aaaaaatagc tgtggtaact agcccgtcag
     7741 gagcggtcat tcgagatatt attacgacgg ttcaacgtcg ttttccgatg agtcaaatcg
     7801 ttctttatcc gacaaaagtt caaggacaag gttcggctga agaaattgct ggaaatatcc
     7861 gtcgagctaa tcaacgtggt gattttgatg tcatgattat tggtcgtggt ggtgggtcta
     7921 tcgaagattt atggggattt aatgaagaaa ttgtggttcg agcaattttt gaatcacgta
     7981 ttccaattat ttcatcggtt ggacatgaaa cagatgtgac tttagctgat tttgttgctg
     8041 atagtcgtgc agcgactcca acagcggcag ccgaattagc aacaccaaat acgaaggttg
     8101 atttaataaa ttggacgaat gagcaggaaa aacgactttt taaccgctta acacatttaa
     8161 ttaaaataag gcgtgaacga gtagataaat taagccagtc agtcgttttt agacagccag
     8221 aacgcttgta cgatggacac ttagaaaaac ttgaccgtct gtgtgaacgt ttgtcagtgc
     8281 tgacggaaaa taaagttgca aatatgaaac accgttatga gctgtcagct gggaaattaa
     8341 ttccgactta cggaaagata gttgaagcta aaaagaataa gacggagcaa ctttatcagt
     8401 ctttactttt attagatatt tcgaaaataa aagcgcgtgg tttttccctc gttactgacg
     8461 aaaagggaaa gattattaaa tccgtaagtg atgtgaaaaa aggtcagact ttagatgttg
     8521 aattgactga cggaaaagta atagtggagg taaaataatg gcaaccaaaa aagaagatgt
     8581 aaaatttgaa gataatttgg cagagttaga aaatattgtt cgtaagttgg aatctggtga
     8641 tgtagcgttg gaagatgcta ttgctgaatt tcaaaaagga atgaaaattt cggaaacttt
     8701 gaaaaaaaca ctcaatgaag cggaagaaac attagtaaaa atagttggaa aaaatgatca
     8761 ggagagtgat ttctctccgg aacagaaaga ctactaaaat aaaaaaacct ttgatttttc
     8821 aaggtttttt attttaaaca aaattttact gacaaaaatg cctattattc taatatcaat
     8881 cttatttatt atatactaat aatagataga gttatggtca agtcttttat agaaagagta
     8941 gattaaatga agaaaaaatt tgtattagcg ccagattctt tcaaagaaag tttgagtgcg
     9001 ctggaagttt gtgaagcaat ggaaaaagga attaaaaaag tatttcctaa ggctgaaata
     9061 atttctatgc caatggctga tggtggtgag ggaacaacag atgctttgat tgatgcaacg
     9121 ggtggacgac gggtatataa aaaagtacaa ggttcatttg caaatacaaa tgtagaggcc
     9181 tattttggta ttttgggaaa ctcaaatacg gcggtcattg agatagcaaa ggcaaatggt
     9241 ctggaattgg ttgctttgga agatagaaat ccattgatta catcaaccta tggaacagga
     9301 caattaatta aggctgcaat ggatgaagga gtagataaaa tcatcattgg cattggtgga
     9361 agtgcgacta atgacggtgg ctctggaatg gctcgtgctt taggagctaa atttttaaat
     9421 aaagaccaag aagagattcc acttggtgga ggttttctta aagaagttag aacaattgat
     9481 atgagtgatt ttgacccaag agtggaagaa attgaaatat taattgctac tgatgtcacc
     9541 aatcctttaa ccggaaataa tggcgcttct tatgtttttg gaccacaaaa gggagcaact
     9601 gaggaaatgg tcaaagtatt ggatgaaaac ttgaagcatt atgcagaaat tgttcataat
     9661 gatctgggga aagatgttaa aaataaaccg ggagctggtg cagcaggtgg tttgggagca
     9721 ggtttgcttt gctttggcag gatatattgg ggatggagca gatgttcttt atgacgaagg
     9781 aatgaccgct atttttggtg ttttgagtca agcacaatct ttgtcagatg ccttggctga
     9841 cggcgcaaag aacgttgagc gaacgagtga aaatattaca agagtattag caatgaatga
     9901 gcaaaaggct cacttataaa aatggaatgg atttgaaaaa taaaaaaatt tcctcaaatc
     9961 atcacggttt ttagccgtga attttggtat aattagggta atggatacga agatactaaa
    10021 gttagaagac tttttaactg aattttatga aagcgctgaa tttccaactg gcttagccga
    10081 atcagcaaaa tattctttat tggcaggcgg taaacgaatt cgccccctcc tttttttaaa
    10141 cctattagag gcttttgatt tagaattgag taaggctcat tatcatgtgg cagccgcact
    10201 tgagatgatt catacgggtt ctttaatcca tgatgactta ccagcaatgg ataatgatga
    10261 ttatcgtcga gggcagttaa ctaatcacaa gaaatttgac gaggcaactg caattttagc
    10321 tggtgatact ttattttttg accctttctt tatcctttca actgctgatt tatccgcaga
    10381 aataatagta gcgttgacgc gtgaattggc ctttgcttca ggttcttatg gaatggttgc
    10441 gggtcaaatc ttggatatgg ctggtgaagg aaaagagttg acattggctg aaattgagca
    10501 gattcatcgc ttgaaaactg ggcgactttt aacttttcct tttgtagctg ctggaattgt
    10561 tgctcaaaaa tcaacggatg aagttgaaaa acttcgtcaa gtgggtcaaa ttttgggatt
    10621 ggcttttcaa attcgagacg atattttaga tgtcacagca acttttgctg agcttggaaa
    10681 aacaccaggt aaggatattt tagaggagaa atcaacatat gttgctcatt taggacttga
    10741 ggaagcaaaa aaatcactga caggaaattt gtcagaagtt aaaaaattac tgacagactt
    10801 atcagttact gacagctctg aaatttttaa aattattgag caattggaag tgaaataaaa
    10861 aaatagaaat ataaaaagaa attattagtt acatttctaa gaaaaatacg tataattact
    10921 ataaagaaag atcaacatga aagaaagaat tgacgtactt gccacgaatc aaggtttgtt
    10981 tgaaacacga gagcaggcca aaagaggagt aatggctggt ctagttgttg atagtaaatc
    11041 aggagagcgt tacgataaac ctggtcagaa aattgatgat ggaacagaac ttcgtcttaa
    11101 aggtgaaaag ttaagatatg ttagtcgagg agggttaaaa ttagaaaagg ctcttaaaga
    11161 attccattta gaaattaatg aaaaaacttg tcttgatatt ggttcttcaa ctggtggttt
    11221 tactgatgta atgcttcaaa atggtgccaa attagtctat gctttagatg ttggaaccaa
    11281 tcaattggct tggaaaattc gctcagatga acgcgttgtc gttatggaac aatttaattt
    11341 ccgaaatgct gttttagctg actttgaaca aggaagaccg tcatttacta gtatagatgt
    11401 tagttttatt tctttagatt taattctacc gccactttat gaaattttgg aagaaaatgg
    11461 cgaagtagca gctcttatta aaccgcagtt tgaagcggga cgtgaacaag tcggaaaaaa
    11521 tggaattatt cgtgacccta aagttcacca aatgactatt gaaaaagttt taaaaacagc
    11581 cactcaactt ggtttttcag taaaaggttt gaccttttcg ccaatcaagg gtggggctgg
    11641 aaacgttgaa tttcttgtcc atttacttaa agatggaaaa gcagaaattg ctcagcaggt
    11701 taatattgaa tcagttttac aaaaagaaag tgaagaacta tgaaaagaga agaccgatta
    11761 aactttattg ctcaatttat ccgtgaaaac gaaattaaaa cgcaagaaga acttgtgaat
    11821 accctcttaa ctcatggaat tgacgttact caagcgaccg tttcccgcga tattaaatca
    11881 cttgccctaa tcaaggtgcc agctgaatct ggtggttatc gttatgattt accaaaaaac
    11941 aaggaagttt tacagtcaag ccttcataaa gccttagctt ttgatgcaat tacgggaaca
    12001 aaaatcaaag ataatatgct ctggattctt gctaatccag ggacaacaag tcttgtgaaa
    12061 aattatcttt tggaagaata tagcgatgat attttttcaa tcattattga tgatgatagt
    12121 gctttagtta tttttgaaat ggaagaagat gctaaaaatc tttataattt actgacagaa
    12181 ttttaaattt actgacaaga atattgtgac aaaattctgc tgaatatttt gagcgaatta
    12241 atagctgtca gtaaaaaaga taaggttatt gggttactaa tagccttgtc atttattttt
    12301 gagaaaggaa attttgatgt tacaagagat ttcaatcaaa aattttgcaa ttattgaaga
    12361 aattcatcta tcctttgaga gtggaatgac gattttaact ggggaaactg gtgcgggaaa
    12421 atcaataatt attgatgcaa tgtctttatt attaggtggg cgtgcttcga gtgattttgt
    12481 gcgacatggg gcaaacaaag ccgaaataga aggtctcttt ttctttgata aaactccaga
    12541 attgaaggcg gtattattag aacttggctt tgaagaagta gattctgaaa ttattttaag
    12601 acgtgagatt tttgctaatg gtcgttccgt ttgtcgaatc aatggtcaaa tggttaattt
    12661 agctacgcta agacaagtgg gtgaattatt agttgatatt catggtcaac acgatagcca
    12721 agaattgatg aatccgaaaa gccatcttcg tttacttgat gaatttggtg acgaagcctt
    12781 tgatcagata aaaaataatt acaaactcaa atttgaaaac ttcaaaaatt tgcgtcaaca
    12841 gctcaattta cgacaaaaaa atgagcaaga atttgctcaa agaattgaaa ttcttcaatt
    12901 tcaggctgaa gaaattgaag cggcggaaat taatcttgaa gaagatgaac aattagttaa
    12961 tcgccgagaa aagttaaata atattaaaaa tattgctgat tcgcttagta gtgcttatct
    13021 agcgcttgat gatgaagata atgattattc aagtcttaat aatatccgaa caacaatgac
    13081 agaattggat aaaattagta aatttgataa tgactatcaa gaacttgctg acaagacagc
    13141 tgaaagttat tatgtattag aagaagttgc taatcaaatt caaagaatca tgagtgattt
    13201 agaatttaat ccagctgaac ttttacaaat tgaagaccgg attatgacct tgaccacttt
    13261 gaagaaaaaa tatggcccag aactgtccga tgtgatgaac tatttagaga aagtccaact
    13321 tgaactctca gaactgacgg gttcagaaaa tgactcagaa aatctagtaa atacagttaa
    13381 agttgcacaa gttcaactca ttgaagcaag taaaaaactt aatgaagcgc gccataaaat
    13441 tgccaaagaa gttgaaaaag atattaaaca cgaactttct gagctttata tggaaaaagc
    13501 tgatttcaaa gttagttttg aaggggcaaa attttcagca aacgggaatg agcatgttga
    13561 attttttatt caaaccaatc cgggagaagg atttaaaccg ctggcaaaaa cagcttcggg
    13621 tggtgaactt tcacgtttaa tgctcgctat aaaatcaagc ttttcaagac gtgaaaataa
    13681 aacagccatt gtttttgatg aggtcgatac tggtgtttct ggtcgtgttg ctcaagcgat
    13741 tgctaataaa atatataaaa ttgctagaca tgggcaagtt cttgcaattt ctcaccttcc
    13801 acaagttgtg gccattgccg atagacaatt ttatattgaa aaagcttcaa ccgatgacat
    13861 tactacttcc acagttagaa aattaaacgc tgaagaaaga gtaaatgaaa ttgcgaaaat
    13921 gcttgctggt gaagatttaa ctaaagaagc cttagctcag gctaaacgtt tattgagcaa
    13981 taaatcgaat attattaaga aaaagtcttg atttttaatc agggcttttt atcttttaaa
    14041 tgacaaaatc gtaattagtg taaaattgca accacaaatg tgtataaacc gaaaataata
    14101 aatgttaaaa ttaaattgta gttacactat taaaaatatt ggagtatatg tgaaagtaaa
    14161 taaaatttgg gttttaatca ttttggtctg tttgtttttt tcaatttatg ggcattataa
    14221 tcaaaattat tttttcttgt ttgtaggtat tgttggtgcc tgtgcgggaa taatctgtat
    14281 gttatgcgaa atgctgatcc aaattcttag aaatcaaaag ataaaaaaat aaattttaaa
    14341 aagctagttt tagataaatt tctttgagtt gttagcaaat gttactgaca agccttttga
    14401 aaaaggctaa aatatcttta tatactatgt gccaacttcc aagtcaaaga gccatagata
    14461 aaaacgcctt ctcctatata agataagggc gtttttatta taaattcgaa gcctcaattt
    14521 attggggctt tttatctcca aaggagatat tatgaagtaa aattagattt tttaccatta
    14581 agaacttctg ttaaagttct tttatgaatt aaactcaata tgttataatg agatatcaac
    14641 catagtactg aaaggagagg tttgtaattt aaaaactgaa gccgtatttt ataatttagg
    14701 agttaaaaat gagtaaaaca cgtttagaag catttacgga tgctgtcatc gcaattatca
    14761 tgactgtctt agttttagaa ttggtccgtc cagattcaga tagcttacaa agttttattg
    14821 ctttacttcc gcatctgggt gtctattttg taagtttttt tatactggct atttactggg
    14881 tcaatcacca tcatcttttt caaagcatca aaagaattaa tggaaaagtt ctatggataa
    14941 atattagttt tctttttgtc ttatcactta ttcctatttt ttctaactgg gtatcaatct
    15001 atcctaattc atttatccca gaattaggct atgtgattat ctttttcttc gggaatttta
    15061 tttatctact tttgacaaga gaattattga aaatcaatgg acatcgtaaa acgtctgaga
    15121 caacgattag aaaaaatatc ctaagtgttg tgctcaatat tgtcagtatt ttattaggtt
    15181 attttatagc tccagtaatc atgcttgttg gttcagcctt aattttttca atgtgggtga
    15241 ttccagataa aaatattgaa aaaatgttta aataatcttt taataaaaat atgagtttac
    15301 ttaataaaaa agtttggaat aaataactga ccttctgtca gtaaataata ttataagtaa
    15361 agaggaataa caatgattaa agcatacatc aaatattgga aaaaagcagg agatttaaaa
    15421 tcttattcaa atcgttcgga ttattggtgg gttttcttgg ctaattcaat catatttgcg
    15481 attctcagca ttttaaactt tatggtgaca atccctaaag ctgggaaaat aatgagccaa
    15541 tctgcaactt tatcacaaaa agaaattatt caacaggtga cagacttata tgccaacccc
    15601 acgggtggag cattagtaat agttattgtt acagctctca ttggtctagc tattcttgtt
    15661 cctaatatca gtttgacagc tcgtcgttta cgtgatgcta ggtttccttg gtggattgct
    15721 cttgtttttg gagtagctgc aatttatggg ctggttacaa tgtttattca tcaagatctt
    15781 ctagcaaaaa tgggttattt tgttggattt atcaatttca ttatttatat tctttgcctt
    15841 tttccaacca aatatggcat tgacgaagaa gatgattcaa gaacttatga ataattttaa
    15901 aaatagagag gaaaaatatg gttcaagctt ataaaaaatt ttggctggga gctttcactt
    15961 tcaatagaaa aactagtcgt aaagattttt ggtcagcctt attaactcat atcataatat
    16021 ttgttatctt gtttaaagct tatcattttt ttaatttatt agatttttat caactaacga
    16081 ctttatggca aacttttgct tcattctttc aattgatatt taacctatat ttctttggaa
    16141 gtttattgtc cttcattgct ctaactgttc gaagattaaa tgatgctgat ttaccttggg
    16201 cacttatttt tcttaatttt atccttgggt taggaactct tgtgctcttg gtacttaacc
    16261 tatttccaag ttcgcctaga gctttaaaat tcaaggaata cgagattaat tcttctcaag
    16321 aatttaataa tttaccagag acaaagacat tatcaggtat attcaaggac tattttaaaa
    16381 attactttga atttcgcgga agaacgacaa gaagaaattt ttggtggatg caactgtttt
    16441 ggggattgac ctttattctt tttctgtttt taatctatct tttcaatcaa tttgaacaaa
    16501 taatgtttgg ctacaatttt attggtagta tggtactgcg tttattattt ttcctattta
    16561 tcttaggaac atttttccca caacttacga ttcatgtaag acgcttaaga gatgctggtt
    16621 tgagtaattt aggactatca cttttgttag gtggaacaag tggaatatta attttttatc
    16681 aaatgttcac gaagacatta aaaataacat atacaactgg tcactatcaa ttagttcaat
    16741 atctcttatt tttactagta atgattgcag tattgagttt aattcttgta gaagtaatgg
    16801 cgacaggaga attaaaaaca aataaaaaaa attctttatt tgaaaaaata gactgattaa
    16861 tttcagtcta tttttagtat taattgttaa actataaaaa gtcacgttta cacaacttgt
    16921 aaattgaatt gacatcaaag aagtcagtaa tagatacaaa tgtagttcat gtaagccttt
    16981 tcattaaaaa aatctttaca taatcatgta aactactaca aaaatagaaa aaaagtgata
    17041 taataaactg taagtataca aggaggacgg cgctcttgcc aaaaacaata gcatttaagc
    17101 atgatacagt cttactacat gaaaccgtag atatgcttga agtaaagcca aatggaattt
    17161 atgttgatgc gacactaggt ggcgcaggac atagtgagta tcttcttagt aaactgactg
    17221 atggtcatct ttattcattt gaccaggatg aaactgctca tgaaaacgca aaagtacgct
    17281 taagtgagca acttgcagaa gataaagtaa cattgattaa aagtaatttt cgctatctta
    17341 aatcagcttt agctgaatta ggagtcacga aaattgatgg aattctttat gatttaggag
    17401 tttccagccc acaattcgat gatagtcaac gaggtttctc ttataaaaaa gaggcaagat
    17461 tagacatgcg gatggaccaa aatcaaacac tttctgctta tgaagttgta aatgaatatc
    17521 cttacgaagc attagttcgc atttttttcc gctacggcga agataaattc tctaaacaaa
    17581 ttgctcgtaa gattgaacaa gcaagaaaaa ttaaacctat tgagacaaca attgagcttg
    17641 cagatttaat aaaatctgca ttgcctcaaa aagaactcaa gaaaaaaggg catccagcca
    17701 aacgcatttt ccaagcgatt cgtattgaag taaatgatga acttggagca gcggaagaat
    17761 caatcgaaca agccattgat ttattaaaag ttgatggtcg aatctcggta attactttcc
    17821 attctttgga agatcgttta acaaaaacaa ttttcaaaga atatagtaca gttaatgttc
    17881 ccaagggatt accgatgctt cctaaagata tggaagctaa actaaaattg attaatcgca
    17941 aaccagttct tgcaagcgaa gaagaactcg aatttaataa tcgagctcat agtgcaaaat
    18001 taagagtagc gcaaaaacaa agagattaat caaataacaa gttggtctaa taaagagggt
    18061 tttcccactt gaggattgtt tttggatgtc ttatataaat gaaataacaa tatttagctt
    18121 ttctcttttc aatggtgaag agagtaagct aaatttcttt agtaagataa acgagtggag
    18181 gcaaagatgg cagcacagcc taaagaatac ttcgatatag aaactgcggc gaaaaaagat
    18241 agttatctca ttgatacgga aactttagcg ccagaagttc tagcaagtaa gtttaaaaag
    18301 ttttctggtg ttgaaaaggt attctatctg acaatggtag ttgctgcttt agtgttggct
    18361 gttggtctac tttatgttaa gactaagaca cttgaagttc aagggaatac aatggactta
    18421 cgttcaaaaa tttctcaaga aacaacccga aaaactgagt atgatcaaca aattttagat
    18481 ttaacatcag gtactaaagt gcttgatacg gcgaataaag ctgatttaaa acaaaatcct
    18541 tctaatgtat tgaaagcaac gaaatgataa aatttttaaa aaaactaatt ttctttcctt
    18601 ttagaaaagt atcacgaaat gcccgaaaaa cgaattggag tacagaaaga aatcgtaaac
    18661 gagtaggtaa gagcctgttt ttcctagcta ttgcgctgtt tacggttttt atatttcgtt
    18721 ttgtctggct aattaccgtc aatcatgtcg gtggaaccaa tcttacaacg atggccaaga
    18781 gcaactatca aagcacagta acggttcagg cgaagcgtgg aacaatatat gaccgtacag
    18841 gtgcggccat tgcggtagac tcaagtacct atacaattta tgctgtaatt gataaaacac
    18901 aggttgattc aaacggaaat cctctttata ttgataagaa agattttacc aaagttgaag
    18961 atttcttgaa tagtaaactt ggaattgacc gtgatttgat taaaaagcag ctcaattcta
    19021 aattgaaaca agttcaattt ggccctaaag ggagtgatat ctctcttgaa aaaatgaaag
    19081 agattcaaaa agcggctgag aatgaaaaaa ttgttggact tggtttcacg gcaaatattt
    19141 ctcgttctta tccttttgga aattttgctt ctcaatttat tggtattgct cgtcctaaag
    19201 atgaaaatgg tactcaagct ctcaagggtg atatgggctt ggaaaaagcc ttcaataatg
    19261 ttttgagtgg tgaaaatggt aaagaaactt atcaaaaaga tatctatggt cgcccaattc
    19321 caggaacaac taaagtcatt gaacctgtta aaaatggtca agacgtttat actacacttg
    19381 atgcacaact tcaaagaaat cttgaaggat atatggacaa agcggctacg gatactggag
    19441 cacaacaact ttcaggaact ttagttgatg ctcataccgg tgagatttta gcaacaagtc
    19501 aacgtccaac ctatacggca acaacgatca atgatgctga gaaacaaaag tatttcactt
    19561 ggaacagttt actctcacaa tcagcctttg aaccgggttc aacatttaaa actttcttga
    19621 tggctggagc gcttgatagt ggaaaagtaa atcttaatga aacttaccaa cggaaacttc
    19681 aagtttatga tacaacgatt aatgactggg atgtaacaga aaataaaagt tatactttgc
    19741 cagaaaccgt aacctatgct caaggttttg ctttatcttc aaatattggg atgagtaaaa
    19801 ttgaaatgaa catgggtaat gatttatggg gaagttatct taataaattt aaatttggtc
    19861 ttaaagtaag agccggttta gatggtgaaa atccgggagc ccttccttca tcgaatgccg
    19921 ttacgcaaat tcaatcttca tttggtcaag gggttgcggt tactccactt caattgataa
    19981 gaggttggac ggctattgct ggaaatggaa caatgcttga gccacatatt gtcagcaaag
    20041 tagtagatcc gaatacaaaa acaagtttga cttctaaagc agaagttgtt ggacatccgg
    20101 tctctaatga tgcagcaagt ggtgtgcgtg atttgatgtt aacagtaaat actgaccctg
    20161 tttacggaac atcttattca acaagtggcg attctgagca aaatcttgct gctggtcctt
    20221 tatttatggt caatggtgaa ccggctgcgg ttaaaacggg gacggcccaa attgcatcaa
    20281 caacgggtgg ttatatgaca ggttctcaag attaccttta ctctgctgtt gtgatgtatc
    20341 ctgctaagaa tcctgatttt attttctata tgaatgtaaa aattcctaca gaaagttgga
    20401 cacttaaata tattgctcga gtggcaaatc cattgttaac aagtgctgaa gcaatgaaaa
    20461 atgatttatc agttagtgct gatactgata ctaaagctgg taaagtgacc attgagaatt
    20521 ataaaggcaa agattcagga gatactgctg atagcttacg ccgtactgtc cttagtccgg
    20581 ttattatcgg gacaggggca aaagtaactg ctcaatcgat tgctgaaggt gaaaaagttt
    20641 cagcaaattc acgaatttta cttttgacga atgataaaga tcaggtgatg cctgatatgt
    20701 atgattggtc gaaaaaagaa gttgagcaat tggctaattg gtttggaatt aaagtaactt
    20761 atgaagggtc aggaaataaa gtcttgactc aaagtattga aacttctacc aatgttaaaa
    20821 aaggtcaaac tctaactgtt aaaatgggaa attaaaaa
//
