GCF_000024545.1 - 21 region(s)

Identified secondary metabolite regions using strictness 'relaxed'

NC_013947.1 (Stackebrandtia nassauensis DSM 44728)

Region	Type	From	To	Similarity Confidence	Most similar known cluster
Region 1	T3PKS,NRPS,T1PKS	127,865	226,181	Low	loseolamycin A1/loseolamycin A2	PKS
Region 2	lanthipeptide-class-iii	545,473	568,034	Medium	catenulipeptin	ribosomal:RiPP:Lanthipeptide
Region 3	lanthipeptide-class-iv	619,322	641,415
Region 4	terpene-precursor	796,978	818,018
Region 5	terpene	1,190,588	1,212,897	High	geosmin	terpene:Sesquiterpene
Region 6	prodigiosin	1,220,758	1,250,320
Region 7	terpene	2,099,818	2,129,308
Region 8	redox-cofactor	2,309,711	2,331,850	Low	lankacidin C	NRPS:Type I+PKS
Region 9	lanthipeptide-class-ii	3,678,950	3,702,159
Region 10	terpene	3,744,170	3,764,955
Region 11	lanthipeptide-class-i	3,817,203	3,841,317
Region 12	RiPP-like	3,862,489	3,872,842
Region 13	NI-siderophore	3,963,971	3,995,332
Region 14	NRPS,T1PKS	4,302,622	4,350,615
Region 15	hydrogen-cyanide,T1PKS	4,372,230	4,443,056
Region 16	lassopeptide	4,526,579	4,549,108
Region 17	NRP-metallophore,NRPS	4,794,873	4,844,603	Medium	griseobactin	NRPS:Type I
Region 18	terpene-precursor	5,327,596	5,348,669
Region 19	lanthipeptide-class-i	5,738,774	5,762,888
Region 20	phosphonate	6,013,721	6,031,783	Low	phosphonoglycans	saccharide
Region 21	ectoine	6,176,106	6,186,525	High	ectoine	other:other

NC_013947.1 - Region 1 - NRPS,T1PKS,T3PKS

Download region GenBank file

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Location: 127,865 - 226,181 nt. (total: 98,317 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxygenase

SNAS_RS00730: Hydroxylation

MITE: MITE0000081Hydroxylation (identity: 63.90%)
smcogs: SMCOG1006: acyl-CoA dehydrogenase

SNAS_RS00795: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

SNAS_RS00830: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

SNAS_RS01055: alcohol dehydrogenase

smcogs: SMCOG1040: alcohol dehydrogenase

Transferases: carboxylase glycosyltransferase methyltransferase

SNAS_RS00615: acetyl-CoA carboxylase biotin carboxylase

smcogs: SMCOG1090: acetyl-CoA carboxylase biotin carboxylase

SNAS_RS00735: methyltransferase

smcogs: SMCOG1089: methyltransferase

SNAS_RS00770: methyltransferase

smcogs: SMCOG1089: methyltransferase

SNAS_RS00900: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

SNAS_RS00965: methyltransferase

smcogs: SMCOG1089: methyltransferase

Hydrolases: esterase hydrolase

SNAS_RS00755: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

SNAS_RS00935: alpha/beta hydrolase fold protein

smcogs: SMCOG1036: alpha/beta hydrolase fold protein

SNAS_RS00955: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: lyase

SNAS_RS00975: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

Ligases: ligase synthase

SNAS_RS00620: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

SNAS_RS00725: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

SNAS_RS00945: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

SNAS_RS00960: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

SNAS_RS32180: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transporter

SNAS_RS00865: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

SNAS_RS01020: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.85	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.76	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.74	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0000465.4	0.73	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002276.2	0.71	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0002135.2	0.70	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.74	NRPS	enniatin	Fusarium equiseti
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0000465.4	0.69	NRPS	xenortide A, xenortide B, xenortide C, xenortide D	Xenorhabdus nematophila ATCC 19061
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001077.4	0.69	PKS	6-anteisoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrogeranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-geranyl-2-methoxyhydroquinone, 6-palmityl-4-O-geranyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-geranyl-2-methoxyhydroquinone, 6-isopalmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-palmityl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-anteisoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone, 6-isoheptadecyl-4-O-dihydrofarnesyl-2-methoxyhydroquinone	Actinoplanes missouriensis 431
BGC0002286.3	0.69	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.24 out of 19.26

Confidence: medium

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 27.32 out of 32.93

Confidence: medium

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.42 out of 19.52

Confidence: strong

Additional weak hits (27):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.47 out of 26.33

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.08 out of 18.57

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.82 out of 27.55

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.70 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 19.15 out of 29.38

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.23 out of 27.78

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.58 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.54 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.04 out of 29.38

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.59 out of 36.54

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.13 out of 26.33

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.01 out of 21.55

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.64 out of 29.65

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.20 out of 39.62

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.64 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.06 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.57 out of 26.06

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 20.35 out of 28.19

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 24.53 out of 32.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.13 out of 35.63

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.03 out of 24.78

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.43 out of 24.78

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.72 out of 32.38

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.33 out of 25.75

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.79 out of 35.63

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.95 out of 24.78

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 127864 - 226181:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (NMe-Ser) + (Gly) + (Leu - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 3, location 167114 - 222991:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (NMe-Ser) + (Gly) + (Leu - X)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 4, location 183016 - 226181:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal) + (NMe-Ser) + (Gly) + (Leu - X)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SNAS_RS00945: Leu - X

Search NORINE for peptide: strict or relaxed

AMP-binding (15..400): Leu

nrpys: Leu

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: leu (Leu)

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDASVWESFLLTGGDVNNYGPTECTVVATS
Nearest Stachelhaus code(s):: Leu DAWFLGNVVK (94% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1052..1437): X

nrpys: Asp, Asn, Glu, Gln, Aad

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAYSFDPAVRELGAFCGGELHGYGPTETTVSVCF
Nearest Stachelhaus code(s):: Arg DPWGFGLVDK (53% 8Å match)
Stachelhaus code match:: 70% (weak)

SNAS_RS00960: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (457..845): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: QAFTFDPVFIEVQALTGGEHNVYGPTETTVWATS
Nearest Stachelhaus code(s):: Gly DILQLGVVWK (68% 8Å match)
Stachelhaus code match:: 80% (moderate)

SNAS_RS00965: Ser

Search NORINE for peptide: strict or relaxed

AMP-binding (494..893): Ser

nrpys: Ser

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Aliphatic chain or phenyl group with -OH (Ser, Thr, Dhpg, Hpg)
Small clusters prediction:: ser (Ser)
Single AA prediction:: ser (Ser)

Stachelhaus prediction details:

Stachelhaus sequence:: RWMTFDVSVWEWHFFTSGDDNLYGPTECAIDVTY
Nearest Stachelhaus code(s):: Ser DVWHFSLIDK (88% 8Å match)
Stachelhaus code match:: 100% (strong)

SNAS_RS32180: mal

PKS_AT (595..876): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 54.6
Methoxymalonyl-CoA: 32.1
Methylmalonyl-CoA: 24.2
inactive: 21.5
fatty_acid: 12.5
CHC-CoA: 9.2
Isobutyryl-CoA: 4.1
trans-1,2-CPDA: 0.0
Propionyl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Benzoyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
RicR	Copper-responsive regulator

NC_013947.1 - Region 2 - lanthipeptide-class-iii

Download region GenBank file

Download region SVG

Location: 545,473 - 568,034 nt. (total: 22,562 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

SNAS_RS02620: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000496.5	0.67	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000513.3	0.61	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.54	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0001226.4	0.53	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.53	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000518.3	0.52	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002700.2	0.51	ribosomal	bacinapeptin	Bacillus nakamurai

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000496.5	0.67	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000513.3	0.61	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.54	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0001226.4	0.53	ribosomal	streptocollin	Streptomyces collinus Tu 365
BGC0000563.4	0.53	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000518.3	0.52	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002700.2	0.51	ribosomal	bacinapeptin	Bacillus nakamurai

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for SNAS_RS02645

SNAS_RS34700

Leader:

MALLDLQGLESPADLSATR

Core:

GGSSGSGHSCPSNLSATLCGGTSGLSVLICH

Core with dehydrations:

GGDhaDhaGDhaGHDhaCysPDhaNLDhaADhbLCysGGDhbDhaGLDhaVLICysH

antiSMASH-DB 4.0 matches for SNAS_RS34700

100.0%

NC_013947.1:

Lanthipeptides: SNAS_RS34700_lanthipeptide

1

GGSSGSGHSCPSNLSATLCGGTSGLSVLICH

31

GGSSGSGHSCPSNLSATLCGGTSGLSVLICH

1

GGSSGSGHSCPSNLSATLCGGTSGLSVLICH

31

90.3%

NZ_FNGF01000002.1:

Lanthipeptides: BLS99_RS06065_lanthipeptide

1

GGSSGSGHSCPSNLSATLCGGTSGLSVLICH

31

GGSS SGHSCPSNLSATLCGGTSGLS+L+CH

1

GGSSASGHSCPSNLSATLCGGTSGLSILLCH

31

87.1%

NZ_FWXV01000003.1:

Lanthipeptides: B9C43_RS55710_lanthipeptide

2

GSSGSGHSCPSNLSATLCGGTSGLSVLICH

31

GSS SGHSCPSNLS TLCGGTSGLS+LICH

1

GSSNSGHSCPSNLSVTLCGGTSGLSLLICH

30

with 60 more sequence matches

Only the first 10 are displayed here.

87.1%

NZ_JAAATY010000001.1:

Lanthipeptides: GC106_RS02675_lanthipeptide

2

GSSGSGHSCPSNLSATLCGGTSGLSVLICH

31

GSS SGHSCPSNLS TLCGGTSGLS+LICH

1

GSSNSGHSCPSNLSLTLCGGTSGLSLLICH

30

83.9%

NZ_RSEB01000004.1and 1 other:

NZ_WIAO01000001.1 Lanthipeptides: GFD30_RS00960_lanthipeptide

Lanthipeptides: EIW28_RS16895_lanthipeptide

4

SGSGHSCPSNLSATLCGGTSGLSVLICH

31

S SGHSCPSNLSATLCGGTSGLSVL+CH

1

SASGHSCPSNLSATLCGGTSGLSVLLCH

28

80.6%

NZ_BNAU01000004.1:

Lanthipeptides: IE290_RS20710_lanthipeptide

4

SGSGHSCPSNLSATLCGGTSGLSVLICH

31

S SG SCPSNLS TLCGGTSGLSVLICH

1

SASGKSCPSNLSVTLCGGTSGLSVLICH

28

80.6%

NZ_CDME01000008.1:

Lanthipeptides: BN1199_RS56280_lanthipeptide

2

GSSGSGHSCPSNLSATLCGGTSGLSVLICH

31

GSS SGHSCPSNLS TLC G+SGLS+LICH

1

GSSNSGHSCPSNLSLTLCDGSSGLSLLICH

30

80.6%

NZ_CP012752.1:

Lanthipeptides: AOZ06_RS57330_lanthipeptide

2

GSSGSGHSCPSNLSATLCGGTSGLSVLICH

31

GSS SGHSCPSNLS TLC G SGLS+LICH

1

GSSNSGHSCPSNLSLTLCNGGSGLSLLICH

30

80.6%

NZ_KB903814.1:

Lanthipeptides: A3G1_RS40055_lanthipeptide

4

SGSGHSCPSNLSATLCGGTSGLSVLICH

31

S SGHSCPSNLS TLCGG+SGLSVLICH

1

STSGHSCPSNLSVTLCGGSSGLSVLICH

28

80.6%

NZ_SGWQ01000004.1:

Lanthipeptides: EV193_RS13500_lanthipeptide

4

SGSGHSCPSNLSATLCGGTSGLSVLICH

31

S SGHSCPSNLS TLCGGTSGLS+LICH

1

SASGHSCPSNLSVTLCGGTSGLSLLICH

28

77.4%

NZ_CP091196.1:

Lanthipeptides: L1857_RS00175_lanthipeptide

5

GSGHSCPSNLSATLCGGTSGLSVLICH

31

SG SCPSNLS TLCGGTSGLSVLICH

1

ASGKSCPSNLSVTLCGGTSGLSVLICH

27

77.4%

NZ_JAGFNP010000002.1:

Lanthipeptides: J5V16_RS05050_lanthipeptide

6

SGHSCPSNLSATLCGGTSGLSVLICH

31

SGHSCPSNLSATLCGGTS LSVL+CH

1

SGHSCPSNLSATLCGGTSSLSVLLCH

26

74.2%

NZ_FNAD01000001.1:

Lanthipeptides: BLQ63_RS02575_lanthipeptide

6

SGHSCPSNLSATLCGGTSGLSVLICH

31

SGHSCPSNLSA LCGGTSGLS+L+CH

2

SGHSCPSNLSAALCGGTSGLSILLCH

27

MIBiG 4.0 matches for SNAS_RS34700

25.8%

BGC0000553.3 (SSGG_RS09925):

Lanthipeptide: SRO15-2212

12

SNLSATLCGGTSGLSVLIC

30

S +S +C +S LSV++C

3

SQVSLLVCEYSS-LSVVLC

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.84 out of 20.01

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.57 out of 20.97

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.41 out of 27.78

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

SNAS_RS34700 - Class III

Cleavage pHMM score: 4.50

RODEO score: 0

Monoisotopic mass: 2665.3 Da

Molecular weight: 2666.9 Da

Number of bridges: 3

Alternative weights: (assuming N unmodified Ser/Thr residues); 2684.9 Da N = 1; 2703.0 Da N = 2; 2721.0 Da N = 3; 2739.0 Da N = 4; 2757.0 Da N = 5; 2775.0 Da N = 6; 2793.1 Da N = 7

TFBS definitions

Regulator	Description

NC_013947.1 - Region 3 - lanthipeptide-class-iv

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Location: 619,322 - 641,415 nt. (total: 22,094 nt) Show pHMM detection rules used

lanthipeptide-class-iv: (cds(LANC_like and Pkinase) and not micKC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase oxygenase

SNAS_RS02965: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

SNAS_RS02985: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002337.2	0.58	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0000563.4	0.51	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000496.5	0.48	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000504.5	0.44	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000553.3	0.43	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0001788.3	0.40	ribosomal	suicin 65	Streptococcus suis
BGC0002260.3	0.40	terpene	trichobrasilenol, xylarenic acid B, brasilane A, brasilane F, brasilane E, brasilane D	Annulohypoxylon truncatum
BGC0000285.5	0.40	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0001294.5	0.40	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002110.3	0.39	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002337.2	0.58	ribosomal	class IV lanthipeptide, SflA	Streptomyces sp. NRRL S-1022
BGC0000563.4	0.51	ribosomal	venezuelin	Streptomyces venezuelae ATCC 10712
BGC0000496.5	0.48	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000504.5	0.44	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000553.3	0.43	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0001788.3	0.40	ribosomal	suicin 65	Streptococcus suis
BGC0002260.3	0.40	terpene	trichobrasilenol, xylarenic acid B, brasilane A, brasilane F, brasilane E, brasilane D	Annulohypoxylon truncatum
BGC0000285.5	0.40	PKS, saccharide	flaviolin, flaviolin rhamnoside, 3,3'-diflaviolin	Saccharopolyspora erythraea
BGC0001294.5	0.40	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002110.3	0.39	ribosomal	birimositide	Streptomyces rimosus subsp. rimosus WC-3908

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (1):

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.23 out of 30.98

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

No core peptides found.

TFBS definitions

Regulator	Description

NC_013947.1 - Region 4 - terpene-precursor

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Location: 796,978 - 818,018 nt. (total: 21,041 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

SNAS_RS03800: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Lyases: dehydratase

SNAS_RS03745: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Ligases: synthetase

SNAS_RS33695: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Translocases: transporter

SNAS_RS03735: sugar-binding lipoprotein

smcogs: SMCOG1143: sugar-binding lipoprotein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002162.3	0.49	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000657.5	0.44	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000677.5	0.41	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000706.3	0.39	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001778.5	0.39	other (other)	showdomycin	Streptomyces showdoensis
BGC0000664.5	0.37	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000666.5	0.37	terpene	xiamycin A	Streptomyces sp. SCSIO 02999
BGC0000665.5	0.36	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000871.4	0.36	other (nucleoside)	VEPE, AEPE, TG-1	Myxococcus xanthus DK 1622

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002162.3	0.49	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000657.5	0.44	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000677.5	0.41	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000706.3	0.39	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0000678.5	0.39	terpene	pentalenolactone	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0001778.5	0.39	other (other)	showdomycin	Streptomyces showdoensis
BGC0000664.5	0.37	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000666.5	0.37	terpene	xiamycin A	Streptomyces sp. SCSIO 02999
BGC0000665.5	0.36	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0000871.4	0.36	other (nucleoside)	VEPE, AEPE, TG-1	Myxococcus xanthus DK 1622

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

SNAS_RS33695: PT_FPPS_like

Domain 1 (55 - 344): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 22.46 out of 27.18

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 18.49 out of 25.31

Confidence: medium

Additional weak hits (4):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 17.44 out of 27.78

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.90 out of 20.01

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.97 out of 31.73

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 23.07 out of 30.22

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
NrdR	Represses ribonucleotide reductase encoding genes

NC_013947.1 - Region 5 - terpene

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Location: 1,190,588 - 1,212,897 nt. (total: 22,310 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

SNAS_RS05545: acyltransferase

smcogs: SMCOG1141: acyltransferase

Hydrolases: glucanase

SNAS_RS05585: Pullulanase, type I

smcogs: SMCOG1297: Pullulanase, type I

Lyases: cyclase

SNAS_RS05575: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002285.2	0.46	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001659.3	0.42	terpene	mangicol A	Fusarium equiseti
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0001743.3	0.39	ribosomal	plantaricyclin	Lactobacillus plantarum
BGC0000522.3	0.38	ribosomal	lacticin Z	Lactococcus lactis
BGC0000497.3	0.37	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0000552.3	0.37	ribosomal	SmbA, SmbB	Streptococcus mutans
BGC0000543.5	0.36	ribosomal	pep5	Staphylococcus epidermidis
BGC0002144.3	0.36	ribosomal	phazolicin	Rhizobium sp. Pop5

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0002285.2	0.46	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001659.3	0.42	terpene	mangicol A	Fusarium equiseti
BGC0001639.3	0.40	ribosomal	klebsidin	Klebsiella pneumoniae
BGC0001743.3	0.39	ribosomal	plantaricyclin	Lactobacillus plantarum
BGC0000522.3	0.38	ribosomal	lacticin Z	Lactococcus lactis
BGC0000497.3	0.37	ribosomal	BHT-Aα, BHT-Aβ	Streptococcus ratti
BGC0000552.3	0.37	ribosomal	SmbA, SmbB	Streptococcus mutans
BGC0000543.5	0.36	ribosomal	pep5	Staphylococcus epidermidis
BGC0002144.3	0.36	ribosomal	phazolicin	Rhizobium sp. Pop5

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

SNAS_RS05575: T1TS + T1TS

Domain 1 (4 - 332): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (337 - 699): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 23.57 out of 32.74

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 22.75 out of 30.98

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.04 out of 23.43

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
FPP	Farnesyl diphosphate

TFBS definitions

Regulator	Description

NC_013947.1 - Region 6 - prodigiosin

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Location: 1,220,758 - 1,250,320 nt. (total: 29,563 nt) Show pHMM detection rules used

prodigiosin: (PPDK_N and AMP-binding and PP-binding and PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase halogenase kinase

SNAS_RS05655: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

SNAS_RS05660: acyltransferase 3

smcogs: SMCOG1088: acyltransferase 3

SNAS_RS05690: halogenase

smcogs: SMCOG1119: halogenase

SNAS_RS05760: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Ligases: ligase synthase

SNAS_RS05695: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

SNAS_RS05700: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	0.61	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.60	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002174.2	0.60	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001273.3	0.60	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.60	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001240.4	0.59	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001284.4	0.58	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000037.4	0.57	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0002154.2	0.57	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001244.3	0.61	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0001276.3	0.60	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0002174.2	0.60	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001273.3	0.60	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.60	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001240.4	0.59	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001284.4	0.58	PKS	alternariol	Parastagonospora nodorum SN15
BGC0000037.4	0.57	PKS	cichorine	Aspergillus nidulans FGSC A4
BGC0002154.2	0.57	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.35 out of 26.85

Confidence: medium

Additional weak hits (15):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.70 out of 26.33

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.93 out of 31.73

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.73 out of 18.57

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.80 out of 26.06

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 18.75 out of 26.12

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.47 out of 28.19

Confidence: weak

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 17.06 out of 20.01

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.69 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.76 out of 29.38

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.07 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.45 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.07 out of 26.06

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.77 out of 18.57

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.56 out of 28.19

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.24 out of 35.63

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

SNAS_RS05695: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (10..401): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YQIFFDGSVHDVFSLFGGEEVLYGPTELTIASTA
Nearest Stachelhaus code(s):: Gly DLHFLCLIAK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
RutR	Regulator of the pyrimidine and purine metabolism

NC_013947.1 - Region 7 - terpene

Download region GenBank file

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Location: 2,099,818 - 2,129,308 nt. (total: 29,491 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

SNAS_RS09780: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: acetyltransferase methyltransferase

SNAS_RS09800: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

SNAS_RS09825: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase

SNAS_RS09830: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: synthase synthetase

SNAS_RS09820: pseudouridine synthase

smcogs: SMCOG1279: pseudouridine synthase

SNAS_RS09850: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

SNAS_RS09860: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000657.5	0.63	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000659.3	0.63	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000658.3	0.61	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001361.3	0.54	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002283.2	0.54	terpene	sodorifen	Serratia plymuthica
BGC0002162.3	0.53	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000677.5	0.47	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000665.5	0.47	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002309.2	0.46	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000666.5	0.45	terpene	xiamycin A	Streptomyces sp. SCSIO 02999

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000657.5	0.63	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0000659.3	0.63	terpene	2-methylisoborneol	Saccharopolyspora erythraea NRRL 2338
BGC0000658.3	0.61	terpene	2-methylisoborneol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001361.3	0.54	terpene	sodorifen	Serratia plymuthica 4Rx13
BGC0002283.2	0.54	terpene	sodorifen	Serratia plymuthica
BGC0002162.3	0.53	terpene	sesterfisherol, sesterfisheric acid, compound 6, compound 7a, compound 5, compound 8	Aspergillus fischeri NRRL 181
BGC0000677.5	0.47	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0000665.5	0.47	other (other), terpene	xiamycin, indosespene, sespenine, dixiamycin A, dixiamycin B, sulfadixiamycin A, sulfadixiamycin B, sulfadixiamycin C	Streptomyces sp. HKI0576
BGC0002309.2	0.46	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0000666.5	0.45	terpene	xiamycin A	Streptomyces sp. SCSIO 02999

Similar known clusters from MIBiG 4.0

Similar subclusters

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
monoterpene		2-methylisoborneol	C11	C1-C6	OH
monoterpene		2-methylenebornane	C11	C1-C6	none

Genes with terpene domains:

SNAS_RS09830: T1TS

Domain 1 (6 - 362): Type 1 terpene synthase

Subtype: 2-Methylisoborneol synthase type

Reactions:: 2-MeGPP → 2-methylisoborneol; 2-MeGPP → 2-methylenebornane

SNAS_RS09860: PT_FPPS_like

Domain 1 (56 - 365): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.68 out of 19.52

Confidence: medium

Additional weak hits (13):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.71 out of 25.88

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 16.21 out of 20.97

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.88 out of 21.55

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.92 out of 27.78

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 21.05 out of 30.97

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 23.65 out of 32.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.41 out of 29.38

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.79 out of 29.38

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.02 out of 28.19

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.08 out of 32.93

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.84 out of 28.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.20 out of 24.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
2-MeGPP	2-methylgeranyl diphosphate
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NC_013947.1 - Region 8 - redox-cofactor

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Location: 2,309,711 - 2,331,850 nt. (total: 22,140 nt) Show pHMM detection rules used

redox-cofactor: (cds(PF04055 and (TIGR04085 or SPASM)) and (TIGR02111 or TIGR03966) and minimum(1, [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE, Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE, Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE, Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE, Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE, Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE, Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE, Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE, Thiaglutamate_B_RRE, Thiaglutamate_G_RRE, Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE, Thurincin_rSAM_RRE, Trifolitoxin_RRE]))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: glycosyltransferase

SNAS_RS10690: glycosyl transferase group 1

smcogs: SMCOG1045: glycosyl transferase group 1

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000601.4	0.45	ribosomal	sporulation killing factor	Bacillus subtilis subsp. subtilis str. 168
BGC0001948.3	0.42	other (other)	naseseazine C, C3-aryl pyrroloindolines	Streptomyces sp.
BGC0002508.2	0.41	other (other)	8-azaguanine	Streptomyces pathocidini
BGC0001100.5	0.36	NRPS, PKS	lankacidin C	Streptomyces rochei
BGC0000706.3	0.34	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0002471.3	0.34	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE
BGC0001440.5	0.34	other (other)	cystargolide A, cystargolide B	Kitasatospora cystarginea
BGC0001778.5	0.32	other (other)	showdomycin	Streptomyces showdoensis
BGC0001366.5	0.32	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0002591.2	0.31	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000601.4	0.45	ribosomal	sporulation killing factor	Bacillus subtilis subsp. subtilis str. 168
BGC0001948.3	0.42	other (other)	naseseazine C, C3-aryl pyrroloindolines	Streptomyces sp.
BGC0002508.2	0.41	other (other)	8-azaguanine	Streptomyces pathocidini
BGC0001100.5	0.36	NRPS, PKS	lankacidin C	Streptomyces rochei
BGC0000706.3	0.34	saccharide	kanamycin	Streptomyces kanamyceticus
BGC0002471.3	0.34	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE
BGC0001440.5	0.34	other (other)	cystargolide A, cystargolide B	Kitasatospora cystarginea
BGC0001778.5	0.32	other (other)	showdomycin	Streptomyces showdoensis
BGC0001366.5	0.32	PKS	BE-24566B, zunyimycin A	Streptomyces sp. FJS31-2
BGC0002591.2	0.31	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.44 out of 23.79

Confidence: medium

Additional weak hits (1):

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.53 out of 27.55

Confidence: weak

RREFinder

TFBS Finder

RRE predictions

: RRE type: PqqD_RRE; Location: 2 - 86; Score: 77.2

TFBS definitions

Regulator	Description
IolR	Regulator of the inositol catabolism

NC_013947.1 - Region 9 - lanthipeptide-class-ii

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Location: 3,678,950 - 3,702,159 nt. (total: 23,210 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

SNAS_RS17200: FAD linked oxidase domain protein

smcogs: SMCOG1138: FAD linked oxidase domain protein

Lyases: cyclase

SNAS_RS17190: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000503.5	0.47	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000504.5	0.46	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000500.5	0.45	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002698.2	0.44	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001210.5	0.43	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000516.4	0.43	ribosomal	geobacillin II	Geobacillus thermodenitrificans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0000554.4	0.48	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000503.5	0.47	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0001579.4	0.46	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000504.5	0.46	ribosomal	cytolysin ClyLl, cytolysin ClyLs	Plasmid pAD1
BGC0000500.5	0.45	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0002698.2	0.44	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566
BGC0001210.5	0.43	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002306.2	0.43	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000516.4	0.43	ribosomal	geobacillin II	Geobacillus thermodenitrificans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for SNAS_RS17190

SNAS_RS17185

Leader:

MSTATITRAWTDVEFRAGLSTEELELVEHPAG

Core:

DLDAELRELYIHEATAQSTNCTKWTGSPNCCC

Core with dehydrations:

DLDAELRELYIHEADhbAQDhaDhbNCysDhbKWDhbGDhaPNCysCysCys

antiSMASH-DB 4.0 matches for SNAS_RS17185

100.0%

NC_013947.1:

Lanthipeptides: SNAS_RS17185_lanthipeptide

1

DLDAELRELYIHEATAQSTNCTKWTGSPNCCC

32

DLDAELRELYIHEATAQSTNCTKWTGSPNCCC

1

DLDAELRELYIHEATAQSTNCTKWTGSPNCCC

32

21.9%

NZ_CP059557.1:

Lassopeptides: H2Q94_RS14010_lassopeptide

11

IHEATAQSTNCTKW

24

EA AQ +NC+ W

6

FFEAPAQHSNCSNW

19

10.7%

NZ_CP016076.1:

Lanthipeptides: allorf_2109745_2109951_lanthipeptide

15

TAQSTNCTKWTGS

27

T Q+ +CT ++GS

3

TGQNPDCTGFSGS

15

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.60 out of 19.26

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (1):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.42 out of 33.73

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

SNAS_RS17185 - Class II

Cleavage pHMM score: -2.60

RODEO score: 0

Monoisotopic mass: 3463.5 Da

Molecular weight: 3465.8 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 3483.8 Da N = 1; 3501.8 Da N = 2

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
VqsM	Regulator of quorum-sensing signalling systems

NC_013947.1 - Region 10 - terpene

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Location: 3,744,170 - 3,764,955 nt. (total: 20,786 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001972.4	0.35	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis
BGC0002429.3	0.23	PKS, terpene	higginsianin B, higginsianin A, higginsianin C, 13-epi-higginsianin C, higginsianin D, higginsianin E	Colletotrichum higginsianum IMI 349063
BGC0000059.5	0.22	PKS	filipin	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002032.4	0.21	PKS	pentamycin	Streptomyces sp. S816
BGC0000138.5	0.20	PKS	rimocidin	Streptomyces diastaticus
BGC0000709.5	0.20	saccharide	neomycin	Streptomyces fradiae ATCC 10745 = DSM 40063
BGC0000069.5	0.20	PKS	gephyronic acid	Archangium violaceum Cb vi76
BGC0002506.3	0.19	PKS	homopiloquinone, piloquinone	Streptomyces sp. FXJ8.102
BGC0001283.3	0.19	PKS	Bisenarsan	Streptomyces lividans 1326

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001972.4	0.35	other (other)	toxoflavin, fervenulin	Streptomyces hiroshimensis
BGC0002429.3	0.23	PKS, terpene	higginsianin B, higginsianin A, higginsianin C, 13-epi-higginsianin C, higginsianin D, higginsianin E	Colletotrichum higginsianum IMI 349063
BGC0000059.5	0.22	PKS	filipin	Streptomyces avermitilis MA-4680 = NBRC 14893
BGC0002032.4	0.21	PKS	pentamycin	Streptomyces sp. S816
BGC0000138.5	0.20	PKS	rimocidin	Streptomyces diastaticus
BGC0000709.5	0.20	saccharide	neomycin	Streptomyces fradiae ATCC 10745 = DSM 40063
BGC0000069.5	0.20	PKS	gephyronic acid	Archangium violaceum Cb vi76
BGC0002506.3	0.19	PKS	homopiloquinone, piloquinone	Streptomyces sp. FXJ8.102
BGC0001283.3	0.19	PKS	Bisenarsan	Streptomyces lividans 1326

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

No product predictions

Genes with terpene domains:

SNAS_RS17490: TS_Pyr4

Domain 1 (4 - 169): Pyr4 type terpene synthase

Subtype: unknown

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 19.75 out of 23.05

Confidence: medium

Additional weak hits (6):

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.84 out of 27.78

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 15.35 out of 26.06

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.12 out of 31.73

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.51 out of 31.73

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 21.69 out of 29.65

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.11 out of 18.57

Confidence: weak

Terpene

TFBS Finder

Glossary

No acronyms to display

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NC_013947.1 - Region 11 - lanthipeptide-class-i

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Location: 3,817,203 - 3,841,317 nt. (total: 24,115 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Lyases: cyclase dehydratase

SNAS_RS17755: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

SNAS_RS17760: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002635.2	0.50	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0001289.5	0.45	ribosomal	penisin	Paenibacillus ehimensis
BGC0000560.5	0.43	ribosomal	subtilomycin	Bacillus subtilis
BGC0000506.4	0.40	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.39	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000529.5	0.34	ribosomal	microbisporicin A2	Microbispora corallina
BGC0002637.3	0.19	NRPS	rimomycin A, rimomycin B, rimomycin C	Streptomyces sp. WAC 06783

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002635.2	0.50	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0001289.5	0.45	ribosomal	penisin	Paenibacillus ehimensis
BGC0000560.5	0.43	ribosomal	subtilomycin	Bacillus subtilis
BGC0000506.4	0.40	ribosomal	entianin	Bacillus subtilis subsp. spizizenii
BGC0000559.4	0.39	ribosomal	subtilin	Bacillus subtilis subsp. spizizenii ATCC 6633
BGC0000529.5	0.34	ribosomal	microbisporicin A2	Microbispora corallina
BGC0002637.3	0.19	NRPS	rimomycin A, rimomycin B, rimomycin C	Streptomyces sp. WAC 06783

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptides - No core peptides found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (2):

ArgR

Description: Regulator of arginine biosynthesis genes
Species: Streptomyces coelicolor
Score: 16.87 out of 20.01

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.50 out of 25.88

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

No core peptides found.

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 139 - 228; Score: 44.1

TFBS definitions

Regulator	Description

NC_013947.1 - Region 12 - RiPP-like

Download region GenBank file

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Location: 3,862,489 - 3,872,842 nt. (total: 10,354 nt) Show pHMM detection rules used

RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

SNAS_RS17925: metallo-beta-lactamase family protein

smcogs: SMCOG1170: metallo-beta-lactamase family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001294.5	0.40	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002316.2	0.37	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000528.5	0.37	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002346.3	0.34	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.32	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000502.5	0.32	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001561.4	0.29	NRPS	curacomycin	Streptomyces curacoi
BGC0001920.4	0.28	ribosomal	siamycin I	Streptomyces sp.
BGC0002015.4	0.28	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001674.5	0.28	ribosomal	MS-271	Streptomyces mirabilis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001294.5	0.40	other (other)	7-prenylisatin	Streptomyces sp. MBT28
BGC0002316.2	0.37	ribosomal	cebulantin	Saccharopolyspora cebuensis
BGC0000528.5	0.37	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0002346.3	0.34	ribosomal	kyamicin	Saccharopolyspora sp.
BGC0000503.5	0.32	ribosomal	cinnamycin	Streptomyces cinnamoneus
BGC0000502.5	0.32	ribosomal	cerecidin, cerecidin A1, cerecidin A2, cerecidin A3, cerecidin A4, cerecidin A5, cerecidin A6, cerecidin A7	Bacillus cereus
BGC0001561.4	0.29	NRPS	curacomycin	Streptomyces curacoi
BGC0001920.4	0.28	ribosomal	siamycin I	Streptomyces sp.
BGC0002015.4	0.28	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0001674.5	0.28	ribosomal	MS-271	Streptomyces mirabilis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 20.58 out of 23.05

Confidence: strong

TFBS Finder

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production

NC_013947.1 - Region 13 - NI-siderophore

Download region GenBank file

Download region SVG

Location: 3,963,971 - 3,995,332 nt. (total: 31,362 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acyltransferase

SNAS_RS18350: acyltransferase

smcogs: SMCOG1141: acyltransferase

Lyases: decarboxylase

SNAS_RS18415: diaminopimelate decarboxylase

smcogs: SMCOG1264: diaminopimelate decarboxylase

Isomerases: isomerase

SNAS_RS18420: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000946.5	0.52	other (other)	vibrioferrin	Vibrio parahaemolyticus
BGC0001478.5	0.51	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002527.2	0.50	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0001408.5	0.49	other (other)	xanthoferrin	Xanthomonas oryzae pv. oryzae KACC 10331
BGC0001499.5	0.48	other (other)	aerobactin	Pantoea ananatis
BGC0000944.5	0.48	other (non-nrp siderophore)	staphyloferrin A	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002681.3	0.46	other (other)	rhizoferrin	Francisella tularensis subsp. tularensis SCHU S4
BGC0000947.5	0.44	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0000943.5	0.42	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002471.3	0.41	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000946.5	0.52	other (other)	vibrioferrin	Vibrio parahaemolyticus
BGC0001478.5	0.51	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002527.2	0.50	other (other)	vibrioferrin	Azotobacter vinelandii CA
BGC0001408.5	0.49	other (other)	xanthoferrin	Xanthomonas oryzae pv. oryzae KACC 10331
BGC0001499.5	0.48	other (other)	aerobactin	Pantoea ananatis
BGC0000944.5	0.48	other (non-nrp siderophore)	staphyloferrin A	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002681.3	0.46	other (other)	rhizoferrin	Francisella tularensis subsp. tularensis SCHU S4
BGC0000947.5	0.44	other (other)	vibrioferrin	Vibrio alginolyticus
BGC0000943.5	0.42	other (other)	staphyloferrin B	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002471.3	0.41	other (other)	baumannoferrin A, baumannoferrin B	Acinetobacter baumannii AYE

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.52 out of 19.26

Confidence: medium

Additional weak hits (5):

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 26.36 out of 36.53

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 19.60 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 23.81 out of 36.54

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.28 out of 36.54

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.93 out of 29.25

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein

NC_013947.1 - Region 14 - NRPS,T1PKS

Download region GenBank file

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Location: 4,302,622 - 4,350,615 nt. (total: 47,994 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

SNAS_RS20020: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

SNAS_RS20055: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

SNAS_RS20075: dehydrogenase

smcogs: SMCOG1072: dehydrogenase

Transferases: kinase

SNAS_RS20050: glycerol kinase

smcogs: SMCOG1210: glycerol kinase

SNAS_RS20070: glycerol kinase

smcogs: SMCOG1210: glycerol kinase

Hydrolases: esterase

SNAS_RS20015: thioesterase

smcogs: SMCOG1004: thioesterase

Ligases: ligase synthase

SNAS_RS20000: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

SNAS_RS20005: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.84	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001132.4	0.76	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.73	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.71	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.69	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342.4	0.69	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.68	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.69	NRPS	AM-toxin	Alternaria alternata
BGC0001128.4	0.69	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.68	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001833.4	0.68	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 23.53 out of 30.25

Confidence: medium

Additional weak hits (7):

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.30 out of 31.73

Confidence: weak

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 15.96 out of 20.97

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 18.16 out of 30.97

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.43 out of 30.25

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.06 out of 21.55

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.35 out of 27.18

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.59 out of 22.40

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 17, location 4302621 - 4350615:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X) + (mal)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SNAS_RS20000: X - X

AMP-binding (20..416): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: IWHMFDAFVTEPALHTGGDVNCYGPSETTVDATA
Nearest Stachelhaus code(s):: Gln DAVQMGCVDK (62% 8Å match); Arg DADDSGCVDK (50% 8Å match)
Stachelhaus code match:: 70% (weak)

AMP-binding (1089..1472): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LWHAFDVSIGEPDLLTGGDLNAYGPTEATVTATS
Nearest Stachelhaus code(s):: Arg DVGDIGAVTK (68% 8Å match)
Stachelhaus code match:: 90% (moderate)

SNAS_RS20005: mal

PKS_AT (517..812): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 134.2
Methoxymalonyl-CoA: 66.3
Methylmalonyl-CoA: 57.5
inactive: 47.5
Propionyl-CoA: 41.1
Ethylmalonyl-CoA: 35.8
2-Methylbutyryl-CoA: 28.4
Isobutyryl-CoA: 23.7
CHC-CoA: 21.0
Benzoyl-CoA: 19.6
Acetyl-CoA: 17.4
trans-1,2-CPDA: 11.6
fatty_acid: 11.6
3-Methylbutyryl-CoA: 0.0

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
ColR	Phenol-responsive regulator

NC_013947.1 - Region 15 - T1PKS,hydrogen-cyanide

Download region GenBank file

Download region SVG

Location: 4,372,230 - 4,443,056 nt. (total: 70,827 nt) Show pHMM detection rules used

hydrogen-cyanide: (Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase reductase

SNAS_RS20255: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

SNAS_RS20265: alkyl hydroperoxide reductase subunit

smcogs: SMCOG1176: alkyl hydroperoxide reductase subunit

SNAS_RS20285: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

SNAS_RS20410: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

Transferases: aminotransferase kinase methyltransferase

SNAS_RS20365: aminotransferase class V

smcogs: SMCOG1139: aminotransferase class V

SNAS_RS20405: methyltransferase

smcogs: SMCOG1089: methyltransferase

SNAS_RS20445: PfkB domain protein

smcogs: SMCOG1276: PfkB domain protein

Hydrolases: phosphatase

SNAS_RS20295: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

Ligases: synthase

SNAS_RS20385: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

SNAS_RS20390: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

SNAS_RS20395: Beta-ketoacyl synthase

smcogs: SMCOG1093: Beta-ketoacyl synthase

SNAS_RS36315: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.43	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.43	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.43	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.37	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.31	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001160.5	0.30	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.30	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001257.3	0.30	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.30	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.30	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.74	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.74	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001244.3	0.74	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000041.3	0.69	PKS	oronofacic acid	Pseudomonas syringae
BGC0001276.3	0.64	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001160.5	0.64	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001164.3	0.64	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001257.3	0.64	PKS	1,3,6,8-tetrahydroxynaphthalene	Nodulisporium sp. ATCC74245
BGC0002191.2	0.64	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0002154.2	0.64	PKS	1,3,5,8-tetrahydroxynapthalene, 1-(alpha-l-(2-O-methyl)-6-deoxymannopyranosyloxy)-3,6,8-trimethoxynaphthalene	Pyricularia oryzae 70-15

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 18.25 out of 18.46

Confidence: strong

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 19.52 out of 19.52

Confidence: strong

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 23.81 out of 29.51

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 25.90 out of 35.63

Confidence: medium

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 24.71 out of 29.65

Confidence: medium

Additional weak hits (8):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.31 out of 21.55

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.27 out of 30.22

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.33 out of 25.75

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 20.35 out of 26.33

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 17.30 out of 21.84

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.56 out of 28.14

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.70 out of 25.88

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.38 out of 28.19

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 18, location 4372229 - 4443056:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ccmal) + (Me-redmal) + (mal) + (Me-mal)
Non-colinear ordering:: SNAS_RS36315 + SNAS_RS20395 + SNAS_RS20390 + SNAS_RS20385

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 20, location 4382572 - 4443056:

Rough prediction of core scaffold based on assumed PKS linker matching; tailoring reactions not taken into account

Polymer prediction:: (Me-mal - Me-ccmal) + (Me-redmal) + (mal) + (Me-mal)
Non-colinear ordering:: SNAS_RS36315 + SNAS_RS20395 + SNAS_RS20390 + SNAS_RS20385

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SNAS_RS36315: mmal - mmal

PKS_AT (99..395): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 75.0%; Ethylmalonyl-CoA: 66.7%; Methoxymalonyl-CoA: 66.7%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 169.6
Ethylmalonyl-CoA: 158.2
Methoxymalonyl-CoA: 135.1
2-Methylbutyryl-CoA: 115.5
Propionyl-CoA: 113.8
trans-1,2-CPDA: 107.5
Malonyl-CoA: 91.4
CHC-CoA: 83.7
Benzoyl-CoA: 61.5
Isobutyryl-CoA: 54.9
fatty_acid: 35.5
3-Methylbutyryl-CoA: 31.4
Acetyl-CoA: 31.3
inactive: 22.7

PKS_AT (1085..1376): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 83.3%; Methoxymalonyl-CoA: 79.2%; Ethylmalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 173.9
Ethylmalonyl-CoA: 159.8
Methoxymalonyl-CoA: 134.3
2-Methylbutyryl-CoA: 93.6
CHC-CoA: 89.0
trans-1,2-CPDA: 86.5
Malonyl-CoA: 79.7
Propionyl-CoA: 75.4
Isobutyryl-CoA: 72.8
Benzoyl-CoA: 40.7
3-Methylbutyryl-CoA: 30.7
Acetyl-CoA: 30.0
fatty_acid: 28.3
inactive: 26.7

SNAS_RS20385: mmal

PKS_AT (565..860): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 83.3%; Malonyl-CoA: 79.2%; Methoxymalonyl-CoA: 75.0%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 179.8
Ethylmalonyl-CoA: 151.9
Methoxymalonyl-CoA: 150.3
2-Methylbutyryl-CoA: 129.6
trans-1,2-CPDA: 107.6
Malonyl-CoA: 95.4
Propionyl-CoA: 94.3
Isobutyryl-CoA: 92.0
CHC-CoA: 72.4
3-Methylbutyryl-CoA: 54.8
Benzoyl-CoA: 49.8
Acetyl-CoA: 42.3
fatty_acid: 26.7
inactive: 25.7

SNAS_RS20390: mal

PKS_AT (565..845): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 100.0%; inactive: 87.5%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 220.7
inactive: 122.9
Methoxymalonyl-CoA: 64.2
Methylmalonyl-CoA: 56.9
Propionyl-CoA: 35.5
fatty_acid: 22.7
Isobutyryl-CoA: 22.6
Acetyl-CoA: 21.7
Ethylmalonyl-CoA: 19.3
2-Methylbutyryl-CoA: 15.6
CHC-CoA: 11.2
Benzoyl-CoA: 10.2
3-Methylbutyryl-CoA: 3.9
trans-1,2-CPDA: 0.0

SNAS_RS20395: mmal

PKS_AT (566..858): mmal

ATSignature: Methylmalonyl-CoA

Top 3 matches:: Methylmalonyl-CoA: 95.8%; Methoxymalonyl-CoA: 87.5%; Ethylmalonyl-CoA: 83.3%

minowa: Methylmalonyl-CoA

Prediction, score:

Methylmalonyl-CoA: 196.7
Ethylmalonyl-CoA: 182.3
Methoxymalonyl-CoA: 150.1
2-Methylbutyryl-CoA: 112.6
Malonyl-CoA: 101.9
trans-1,2-CPDA: 99.4
CHC-CoA: 93.2
Propionyl-CoA: 90.4
Isobutyryl-CoA: 77.5
Benzoyl-CoA: 53.3
Acetyl-CoA: 51.4
3-Methylbutyryl-CoA: 46.4
fatty_acid: 42.3
inactive: 28.2

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
CRP	Cyclic AMP receptor protein
DosR	Hypoxia stress response regulator
LexA variant 1	Repressor of DNA damage response
NrtR	NAD synthesis repressor
RpoN	Regulator of nitrogen assimilation and virulence

NC_013947.1 - Region 16 - lassopeptide

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Location: 4,526,579 - 4,549,108 nt. (total: 22,530 nt) Show pHMM detection rules used

lassopeptide: (PF13471 and Asn_synthase or micJ25 or mcjC)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lasso peptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

SNAS_RS20935: asparagine synthase (glutamine-hydrolyzing)

smcogs: SMCOG1177: asparagine synthase (glutamine-hydrolyzing)

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001307.3	0.56	ribosomal	chaxapeptin	Streptomyces leeuwenhoekii
BGC0001548.4	0.54	ribosomal	citrulassin A	Streptomyces albulus
BGC0001655.3	0.53	ribosomal	LP2006	Nocardiopsis alba DSM 43377
BGC0001496.3	0.49	ribosomal	actinokineosin	Actinokineospora spheciospongiae
BGC0001645.3	0.49	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0001889.2	0.46	ribosomal	fusilassin	Thermobifida fusca YX
BGC0002015.4	0.46	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000579.4	0.41	ribosomal	SSV-2083	Streptomyces sviceus ATCC 29083
BGC0001920.4	0.40	ribosomal	siamycin I	Streptomyces sp.
BGC0001674.5	0.40	ribosomal	MS-271	Streptomyces mirabilis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001307.3	0.56	ribosomal	chaxapeptin	Streptomyces leeuwenhoekii
BGC0001548.4	0.54	ribosomal	citrulassin A	Streptomyces albulus
BGC0001655.3	0.53	ribosomal	LP2006	Nocardiopsis alba DSM 43377
BGC0001496.3	0.49	ribosomal	actinokineosin	Actinokineospora spheciospongiae
BGC0001645.3	0.49	ribosomal	lagmysin	Streptomyces sp. NRRL S-118
BGC0001889.2	0.46	ribosomal	fusilassin	Thermobifida fusca YX
BGC0002015.4	0.46	ribosomal	aborycin	Streptomyces sp. ZS0098
BGC0000579.4	0.41	ribosomal	SSV-2083	Streptomyces sviceus ATCC 29083
BGC0001920.4	0.40	ribosomal	siamycin I	Streptomyces sp.
BGC0001674.5	0.40	ribosomal	MS-271	Streptomyces mirabilis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lasso peptide predictions

Lassopeptide(s) for SNAS_RS20930

SNAS_RS20930

Leader:

MRPVLAPGTCYTDLPDGGAALVTPTGGDVVHLDAVAAAFVKALSEHERDTAVTVLAEHFDVSTTTI

Full core:

AADLDDFTATLVDYGVMTGGAS

Putative macrolactam:

antiSMASH-DB 4.0 matches for SNAS_RS20930

100.0%

NC_013947.1:

Lassopeptides: SNAS_RS20930_lassopeptide

1

AADLDDFTATLVDYGVMTGGAS

22

AADLDDFTATLVDYGVMTGGAS

1

AADLDDFTATLVDYGVMTGGAS

22

MIBiG 4.0 matches for SNAS_RS20930

9.8%

BGC0002120.3 (NQE92413.1):

Lanthipeptide: nocathioamide A

5

DDFTATLVDYGVMTGGAS

22

DDF ++D + +S

16

DDFDLNIIDSSMFDDESS

33

Lassopeptide(s) for SNAS_RS37230

SNAS_RS37230

Leader:

MNTKKTPARRTRYARPTVTLLGDAVKLTN

Full core:

GGQYNDTHDSRQYYY

Putative macrolactam:

GGQYNDTHD

antiSMASH-DB 4.0 matches for SNAS_RS37230

100.0%

NC_013947.1:

Lassopeptides: allorf_4539121_4539270_lassopeptide

1

GGQYNDTHDSRQYYY

15

GGQYNDTHDSRQYYY

1

GGQYNDTHDSRQYYY

15

46.7%

NC_015951.1:

Lassopeptides: STRVI_RS44745_lassopeptide

1

GGQYNDTHDSRQY

13

G QY D+ D QY

1

GTQYQDSADDTQY

13

46.7%

NZ_JACEIQ010000003.1:

Lassopeptides: H1191_RS05090_lassopeptide

1

GGQYNDTHDSRQYYY

15

G + DT D ++YYY

1

GDEERDTADLKEYYY

15

with 3 more sequence matches

40.0%

NZ_JALMDK010000025.1:

Lassopeptides: MZC64_RS46020_lassopeptide

1

GGQYNDTHDSRQYYY

15

G ++DT + R YY+

1

GSAFDDTMEKRSYYF

15

40.0%

NZ_KB913024.1:

Lassopeptides: ACTMO_RS22770_lassopeptide

1

GGQYNDTHDSRQYYY

15

G ++DT D +++YY

1

GTSFDDTADRKRWYY

15

33.3%

NZ_CP014168.1:

Lassopeptides: J0A91_RS18750_lassopeptide

1

GGQYNDTHDSRQY

13

G ++DT D RQ

3

GAPFSDTGDGRQL

15

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.61 out of 26.33

Confidence: weak

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 19.59 out of 33.73

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.57 out of 21.55

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 18.42 out of 29.25

Confidence: weak

Lasso peptides

RREFinder

TFBS Finder

Core peptide predictions

SNAS_RS20930 - Class II: Cleavage pHMM score: -7.70; RODEO score: 19; Monoisotopic mass: 2171.0 Da; Molecular weight: 2172.3 Da; Number of bridges: 0
SNAS_RS37230 - Class II: Cleavage pHMM score: 3.20; RODEO score: 21; Monoisotopic mass: 1847.7 Da; Molecular weight: 1848.8 Da; Number of bridges: 0

RRE predictions

: RRE type: Stand_Alone_Lasso_RRE; Location: 10 - 83; Score: 28.1

TFBS definitions

Regulator	Description

NC_013947.1 - Region 17 - NRP-metallophore,NRPS

Download region GenBank file

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Location: 4,794,873 - 4,844,603 nt. (total: 49,731 nt) Show pHMM detection rules used

NRP-metallophore: (VibH_like or Cy_tandem or (cds(Condensation and AMP-binding) and ((IBH_Asp and not SBH_Asp) or IBH_His or TBH_Asp or CyanoBH_Asp1 or CyanoBH_Asp2 or IPL or SalSyn or (EntA and EntC) or (GrbD and GrbE) or (FbnL and FbnM) or PvdO or PvdP or (Orn_monoox and not (KtzT or MetRS-like)) or Lys_monoox or VbsL)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

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TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

SNAS_RS22235: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

SNAS_RS22285: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Transferases: formyltransferase

SNAS_RS22220: methionyl-tRNA formyltransferase

smcogs: SMCOG1228: methionyl-tRNA formyltransferase

Hydrolases: hydrolase

SNAS_RS22205: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

SNAS_RS22300: isochorismatase

smcogs: SMCOG1027: isochorismatase

Lyases: dehydratase

SNAS_RS22255: NAD-dependent epimerase/dehydratase

smcogs: SMCOG1010: NAD-dependent epimerase/dehydratase

Isomerases: isomerase

SNAS_RS22290: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

SNAS_RS22295: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

SNAS_RS22335: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

SNAS_RS33115: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Translocases: permease

SNAS_RS22350: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000343.5	0.78	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002492.3	0.58	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0001135.5	0.58	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001185.5	0.56	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0002324.3	0.54	NRPS, PKS	myxochelin B, myxochelin N, myxochelin O, myxochelin P, myxochelin Q, myxochelin A	Archangium violaceum
BGC0002276.2	0.54	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001345.5	0.53	NRPS	myxochelin A, myxochelin B	Stigmatella aurantiaca Sg a15
BGC0002474.2	0.52	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0000401.5	0.49	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002494.3	0.47	NRPS	vibriobactin	Vibrio cholerae

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000343.5	0.71	NRPS	enterobactin	Pseudomonas sp. J465
BGC0002492.3	0.63	NRPS	myxochelin B, myxochelin A	Myxococcus xanthus DK 1622
BGC0001135.5	0.62	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001185.5	0.62	NRPS	bacillibactin	Bacillus velezensis FZB42
BGC0002324.3	0.61	NRPS, PKS	myxochelin B, myxochelin N, myxochelin O, myxochelin P, myxochelin Q, myxochelin A	Archangium violaceum
BGC0002276.2	0.60	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0001345.5	0.60	NRPS	myxochelin A, myxochelin B	Stigmatella aurantiaca Sg a15
BGC0002474.2	0.60	NRPS	agrobactin	Agrobacterium tumefaciens
BGC0000401.5	0.58	NRPS	paenibactin	Paenibacillus elgii B69
BGC0002494.3	0.57	NRPS	vibriobactin	Vibrio cholerae

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 20.58 out of 23.05

Confidence: strong

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 18.33 out of 19.52

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 17.55 out of 19.26

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 33.07 out of 36.53

Confidence: strong

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.07 out of 18.16

Confidence: strong

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 34.36 out of 36.53

Confidence: strong

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (8):

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.29 out of 18.57

Confidence: weak

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.94 out of 19.26

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.71 out of 27.78

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.17 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.41 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.38 out of 36.53

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.61 out of 35.63

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.65 out of 23.43

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 22, location 4794872 - 4844603:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Lys)

Direct lookup in NORINE database: strict or relaxed

Link to NORINE database query form

NRPS/PKS substrate predictions

SNAS_RS22295: diOH-Bz

Search NORINE for peptide: strict or relaxed

AMP-binding (31..422): diOH-Bz

nrpys: diOH-Bz

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aromatic (Phe, Tyr, diOH-Bz, Ph-Gly, bOH-Tyr)
Large clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Small clusters prediction:: Hydroxy-benzoic acid derivates (diOH-Bz, Sal)
Single AA prediction:: dhb (diOH-Bz)

Stachelhaus prediction details:

Stachelhaus sequence:: SVALFPLSSPGGAVQVGGAQQVFGMAEGLVCYTR
Nearest Stachelhaus code(s):: diOH-Bz or Sal PLPAQGVVNK (88% 8Å match); diOH-Bz PLPAQGVVNK (85% 8Å match)
Stachelhaus code match:: 90% (moderate)

SNAS_RS33115: Lys

Search NORINE for peptide: strict or relaxed

AMP-binding (416..825): Lys

nrpys: Lys

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FSAAFDAHVEEVDLIIGGEINSYGPTEATVVCLA
Nearest Stachelhaus code(s):: Lys DAEDIGSVVK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

Link to PARAS substrate predictor

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
DmdR1	Iron(II)-dependent repressor
VqsM	Regulator of quorum-sensing signalling systems

NC_013947.1 - Region 18 - terpene-precursor

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Location: 5,327,596 - 5,348,669 nt. (total: 21,074 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Ligases: ligase synthetase

SNAS_RS24855: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

SNAS_RS24895: carboxylate-amine ligase

smcogs: SMCOG1165: carboxylate-amine ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002111.3	0.42	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139
BGC0001485.4	0.42	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000486.4	0.40	ribosomal	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001456.3	0.39	terpene	isorenieratene	Streptomyces argillaceus
BGC0000664.5	0.39	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0002111.3	0.42	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139
BGC0001485.4	0.42	other (other)	chuangxinmycin	Actinoplanes tsinanensis
BGC0002306.2	0.42	ribosomal	freyrasin	Paenibacillus polymyxa ATCC 842
BGC0000528.5	0.40	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0000486.4	0.40	ribosomal	butyrivibriocin AR10	Butyrivibrio fibrisolvens
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0001456.3	0.39	terpene	isorenieratene	Streptomyces argillaceus
BGC0000664.5	0.39	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001911.3	0.38	terpene	raimonol	Streptomyces anulatus

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

SNAS_RS24855: PT_FPPS_like

Domain 1 (45 - 351): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.33 out of 36.53

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.76 out of 23.05

Confidence: medium

Additional weak hits (7):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.56 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.57 out of 27.78

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.02 out of 21.55

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.53 out of 32.93

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.83 out of 24.78

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.57 out of 36.53

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 19.10 out of 25.88

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
DmdR1	Iron(II)-dependent repressor

NC_013947.1 - Region 19 - lanthipeptide-class-i

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Location: 5,738,774 - 5,762,888 nt. (total: 24,115 nt) Show pHMM detection rules used

lanthipeptide-class-i: (((Lant_dehydr_N or Lant_dehydr_C) and cds(LANC_like and not Pkinase)) and not (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

SNAS_RS26770: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase dehydratase

SNAS_RS26775: Lantibiotic dehydratase domain protein

smcogs: SMCOG1155: Lantibiotic dehydratase domain protein

SNAS_RS33385: Lanthionine synthetase C family protein

smcogs: SMCOG1140: Lanthionine synthetase C family protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001289.5	0.48	ribosomal	penisin	Paenibacillus ehimensis
BGC0000560.5	0.45	ribosomal	subtilomycin	Bacillus subtilis
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000542.3	0.44	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.44	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000514.5	0.39	ribosomal	gallidermin	Staphylococcus gallinarum
BGC0002120.3	0.36	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0000796.3	0.27	saccharide	S-layer glycan	Paenibacillus alvei
BGC0001435.4	0.25	NRPS, PKS	ikarugamycin	Streptomyces sp. ZJ306
BGC0000700.5	0.24	saccharide	istamycin	Streptomyces tenjimariensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001289.5	0.48	ribosomal	penisin	Paenibacillus ehimensis
BGC0000560.5	0.45	ribosomal	subtilomycin	Bacillus subtilis
BGC0002635.2	0.44	ribosomal	daspyromycin A, daspyromycin B	Actinokineospora diospyrosa
BGC0000542.3	0.44	ribosomal	paenicidin B	Paenibacillus terrae
BGC0000541.5	0.44	ribosomal	paenicidin A	Paenibacillus polymyxa
BGC0000514.5	0.39	ribosomal	gallidermin	Staphylococcus gallinarum
BGC0002120.3	0.36	ribosomal	nocathioamide A	Nocardia terpenica IFM 0706
BGC0000796.3	0.27	saccharide	S-layer glycan	Paenibacillus alvei
BGC0001435.4	0.25	NRPS, PKS	ikarugamycin	Streptomyces sp. ZJ306
BGC0000700.5	0.24	saccharide	istamycin	Streptomyces tenjimariensis

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for SNAS_RS26775

SNAS_RS34480

Leader:

MKPTLEATTDIEAFEDLFKLDVRIEAEGPSLSRCNPT

Core:

NDGCSNSCTSVGCSANC

Core with dehydrations:

NDGCysDhaNDhaCysDhbDhaVGCysDhaANCys

antiSMASH-DB 4.0 matches for SNAS_RS34480

100.0%

NC_013947.1:

Lanthipeptides: SNAS_RS34480_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

NDGCSNSCTSVGCSANC

1

NDGCSNSCTSVGCSANC

17

65.0%

NC_013947.1:

Lanthipeptides: SNAS_RS34155_lanthipeptide

1

NDGCSNSCTSVGCS

14

NDGCSNSCTSVGC

6

NDGCSNSCTSVGCP

19

58.3%

NZ_CP043661.1:

Lanthipeptides: F1D05_RS10920_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

NDGCS SC SVGCSA C

3

NDGCSASCPSVGCSATC

19

with 26 more sequence matches

Only the first 10 are displayed here.

42.9%

NZ_JACHJO010000003.1:

Lanthipeptides: FHS13_RS04620_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

NDGC +C S CS +C

2

NDGCGKTCQSA-CSPSC

17

41.2%

NZ_SPFR01000005.1:

Lanthipeptides: FGQ83_RS17540_lanthipeptide

1

NDGCSNSCTSVGCS

14

GCS CT + CS

3

TKGCSQFCTIICCS

16

39.1%

NZ_CP047146.1and 3 others:

NZ_CP047146.1 Lanthipeptides: GR130_RS38445_lanthipeptideNZ_CP048875.1 Lanthipeptides: G3260_RS00640_lanthipeptideNZ_CP048875.1 Lanthipeptides: G3260_RS00640_lanthipeptide

Lanthipeptides: GR130_RS38445_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

+DGC +C S CSA C

7

DDGCGQTCES-ACSATC

22

39.1%

NZ_JABBWZ010000004.1and 4 others:

NZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS10860_lanthipeptideNZ_JABBWZ010000004.1 Lanthipeptides: HHX40_RS10860_lanthipeptideNZ_VYTZ01000008.1 Lanthipeptides: F5972_RS22705_lanthipeptideNZ_VYTZ01000008.1 Lanthipeptides: F5972_RS22705_lanthipeptide

Lanthipeptides: HHX40_RS10860_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

+DGC SC S C A C

7

DDGCGTSCQS-ACPATC

22

39.1%

NZ_JACHGT010000013.1and 1 other:

NZ_JACHGT010000013.1 Lanthipeptides: HNR73_RS27205_lanthipeptide

Lanthipeptides: HNR73_RS27205_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

NDGC +C S CS C

7

NDGCGQTCQS-ACSTTC

22

34.8%

NC_013131.1:

Lanthipeptides: allorf_05058087_05058206_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

N GC + TSV A+C

1

NSGCRHRITSVESRASC

17

34.8%

NZ_CP054916.1and 1 other:

NZ_CP054916.1 Lanthipeptides: HUT19_RS32830_lanthipeptide

Lanthipeptides: HUT19_RS32830_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

+DGC +C S CS C

7

DDGCGQTCQS-ACSTTC

22

34.8%

NZ_JAFIQY010000070.1and 1 other:

NZ_JAFIQY010000070.1 Lanthipeptides: JXZ78_RS39125_lanthipeptide

Lanthipeptides: JXZ78_RS39125_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

+DGC +C S CS C

7

DDGCGQTCES-ACSTTC

22

33.3%

NZ_BMRB01000002.1and 1 other:

NZ_CP031087.1 Lanthipeptides: Actkin_RS21245_lanthipeptide

Lanthipeptides: IEY98_RS16230_lanthipeptide

1

NDGCSNSCTSVGCSANC

17

NDGC ++C S C+A+C

7

NDGCGHTCDSA-CAASC

22

30.4%

NZ_BMSK01000006.1:

Lanthipeptides: IE251_RS13810_lanthipeptide

1

NDGCSNSCTSVGCS

14

GCS CT V C+

4

TQGCSGLCTIVICA

17

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 26.27 out of 27.18

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 17.13 out of 18.57

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.80 out of 20.47

Confidence: medium

Additional weak hits (3):

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.01 out of 29.65

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 21.06 out of 29.25

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.99 out of 33.14

Confidence: weak

Lanthipeptides

RREFinder

TFBS Finder

Core peptide predictions

SNAS_RS34480 - Class I

Cleavage pHMM score: -5.10

RODEO score: 19

Monoisotopic mass: 1530.5 Da

Molecular weight: 1531.6 Da

Number of bridges: 4

Alternative weights: (assuming N unmodified Ser/Thr residues); 1549.6 Da N = 1

RRE predictions

: RRE type: Lanthipeptide_LanB_RRE; Location: 138 - 227; Score: 43.6

TFBS definitions

Regulator	Description
NrdR	Represses ribonucleotide reductase encoding genes
ToxT	Regulator of MSHA biosynthesis
VqsM	Regulator of quorum-sensing signalling systems

NC_013947.1 - Region 20 - phosphonate

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Location: 6,013,721 - 6,031,783 nt. (total: 18,063 nt) Show pHMM detection rules used

phosphonate: (phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase or phosphonatesMeIsocitrateLyase))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidase

SNAS_RS28020: pyruvate oxidase/decarboxylase

smcogs: SMCOG1055: pyruvate oxidase/decarboxylase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000807.3	0.74	saccharide	phosphonoglycans	Stackebrandtia nassauensis DSM 44728
BGC0000938.5	0.47	other (aminocoumarin)	fosfomycin	Streptomyces fradiae
BGC0001739.3	0.41	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525
BGC0002670.2	0.40	other (other)	fosfonochlorin	Fusarium oxysporum f. sp. lycopersici 4287
BGC0000616.4	0.39	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000806.5	0.39	saccharide	phosphonoglycans	Glycomyces sp. NRRL B-16210
BGC0000897.5	0.39	other (other)	dehydrophos	Streptomyces luridus
BGC0002747.3	0.38	ribosomal	lentinulin A	Lentinula edodes
BGC0001684.3	0.37	other (other)	N-acyloxyacyl glutamine	Klebsiella variicola
BGC0001655.3	0.36	ribosomal	LP2006	Nocardiopsis alba DSM 43377

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000807.3	0.74	saccharide	phosphonoglycans	Stackebrandtia nassauensis DSM 44728
BGC0000938.5	0.47	other (aminocoumarin)	fosfomycin	Streptomyces fradiae
BGC0001739.3	0.41	other (other)	phosphonoacetic Acid	Streptomyces sp. NRRL F-525
BGC0002670.2	0.40	other (other)	fosfonochlorin	Fusarium oxysporum f. sp. lycopersici 4287
BGC0000616.4	0.39	ribosomal	amylocyclicin	Bacillus velezensis FZB42
BGC0000806.5	0.39	saccharide	phosphonoglycans	Glycomyces sp. NRRL B-16210
BGC0000897.5	0.39	other (other)	dehydrophos	Streptomyces luridus
BGC0002747.3	0.38	ribosomal	lentinulin A	Lentinula edodes
BGC0001684.3	0.37	other (other)	N-acyloxyacyl glutamine	Klebsiella variicola
BGC0001655.3	0.36	ribosomal	LP2006	Nocardiopsis alba DSM 43377

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.92 out of 20.47

Confidence: medium

Additional weak hits (5):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.18 out of 21.55

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.94 out of 24.78

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 18.89 out of 23.43

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 18.50 out of 36.54

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.50 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
VqsM	Regulator of quorum-sensing signalling systems

NC_013947.1 - Region 21 - ectoine

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Location: 6,176,106 - 6,186,525 nt. (total: 10,420 nt) Show pHMM detection rules used

ectoine: ectoine_synt

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

SNAS_RS28765: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002052.3	0.81	other (ectoine)	ectoine	Streptomyces sp.
BGC0000853.3	0.81	other (other)	ectoine	Streptomyces anulatus
BGC0000855.3	0.72	other (other)	ectoine	Methylomicrobium kenyense
BGC0000856.3	0.72	other (other)	ectoine	Methylophaga thalassica
BGC0000859.3	0.72	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000852.5	0.70	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.67	other (other)	ectoine	Methylobacter marinus
BGC0000857.3	0.64	other (other)	ectoine	Methylophaga alcalica
BGC0000854.3	0.63	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0000860.3	0.50	other (other)	ectoine	Methylarcula marina

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002052.3	0.81	other (ectoine)	ectoine	Streptomyces sp.
BGC0000853.3	0.81	other (other)	ectoine	Streptomyces anulatus
BGC0000855.3	0.72	other (other)	ectoine	Methylomicrobium kenyense
BGC0000856.3	0.72	other (other)	ectoine	Methylophaga thalassica
BGC0000859.3	0.72	other (other)	ectoine	Methylomicrobium alcaliphilum
BGC0000852.5	0.70	other (other)	ectoine	Sporosarcina pasteurii
BGC0000858.3	0.67	other (other)	ectoine	Methylobacter marinus
BGC0000857.3	0.64	other (other)	ectoine	Methylophaga alcalica
BGC0000854.3	0.63	other (other)	ectoine	Methylomicrobium alcaliphilum 20Z
BGC0000860.3	0.50	other (other)	ectoine	Methylarcula marina

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (4):

HipB

Description: Bacterial antitoxin HipB
Species: Escherichia coli BL21(DE3)
Score: 20.94 out of 33.73

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 19.97 out of 26.33

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 19.58 out of 30.25

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 17.10 out of 29.38

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description