LOCUS       NC_013947              10420 bp    DNA     circular CON 23-APR-2024
DEFINITION  Stackebrandtia nassauensis DSM 44728, complete sequence.
ACCESSION   NC_013947
VERSION     NC_013947.1
KEYWORDS    RefSeq.
SOURCE      Stackebrandtia nassauensis DSM 44728
  ORGANISM  Stackebrandtia nassauensis DSM 44728
            Bacteria; Actinomycetota; Actinomycetes; Glycomycetales;
            Glycomycetaceae; Stackebrandtia.
REFERENCE   1  (bases 1 to 6841557)
  AUTHORS   Munk,C., Lapidus,A., Copeland,A., Jando,M., Mayilraj,S., Glavina Del
            Rio,T., Nolan,M., Chen,F., Lucas,S., Tice,H., Cheng,J.F., Han,C.,
            Detter,J.C., Bruce,D., Goodwin,L., Chain,P., Pitluck,S., Goker,M.,
            Ovchinikova,G., Pati,A., Ivanova,N., Mavromatis,K., Chen,A.,
            Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
            Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C.
            and Klenk,H.P.
  TITLE     Complete genome sequence of Stackebrandtia nassauensis type strain
            (LLR-40K-21)
  JOURNAL   Stand Genomic Sci 1 (3), 234-241 (2009)
   PUBMED   21304662
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841557)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
            Mavromatis,K., Ivanova,N., Ovchinnikova,G., Munk,A.C., Brettin,T.,
            Detter,J.C., Han,C., Tapia,R., Larimer,F., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,M.,
            Mayilraj,S., Goker,M., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     The complete genome of Stackebrandtia nassauensis DSM 44728
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6841557)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
            Mavromatis,K., Ivanova,N., Ovchinnikova,G., Munk,A.C., Brettin,T.,
            Detter,J.C., Han,C., Tapia,R., Larimer,F., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,M.,
            Mayilraj,S., Goker,M., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2009) US DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000024545.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 01:07:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,519
            CDSs (total)                      :: 6,464
            Genes (coding)                    :: 6,414
            CDSs (with protein)               :: 6,414
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 0 of 50
            Pseudo Genes (incomplete)         :: 50 of 50
            Pseudo Genes (internal stop)      :: 0 of 50
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##Metadata-START##
            Organism Display Name :: Stackebrandtia nassauensis LLR-40K-21, DSM
            44728
            Culture Collection ID             :: DSM 44728
            GOLD Stamp ID                     :: Gc01107
            Funding Program                   :: DOE-GEBA 2007
            Number of Reads :: 81931 Sanger, 851638 pyrosequence
            Assembly Method :: Newbler version 1.1.02.15, Phrap
            Sequencing Depth                  :: 11x Sanger; 29x 454
            Gene Calling Method               :: Prodigal 1.4, GenePRIMP
            Isolation Site                    :: Soil sample from Nassau Bahamas
            Isolation Country                 :: Bahamas
            Latitude                          :: 25.066127
            Longitude                         :: -77.339025
            Oxygen Requirement                :: Aerobe
            Motility                          :: Nonmotile
            Sporulation                       :: Nonsporulating
            Temperature Range                 :: Mesophile
            Temperature Optimum               :: 28C
            Gram Staining                     :: gram+
            Biotic Relationship               :: Free living
            Diseases                          :: None
            Habitat                           :: Soil
            ##Metadata-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:48:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6176105
            Orig. end                         :: 6186525
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP001778.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4082381
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Annotation by JGI-ORNL and JGI-PGF
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10420
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="21"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10420
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10420
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5420](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5420
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            1197..1895
                     /locus_tag="SNAS_RS28750"
                     /old_locus_tag="Snas_5809"
     CDS             1197..1895
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SNAS_RS28750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Snas_5809"
                     /product="hypothetical protein"
                     /protein_id="WP_013021010.1"
                     /transl_table=11
                     /translation="MRIRAIAAMGLAAAALLTVSACRVEQGAALFVGDERISEKRVDEV
                     VDSISASDRAKLADLNQLTGGQLPDISTGNLRGYVVDALVVTELGQRVAADTGVKPDRE
                     SSSVIKKNWELLELDSDSEFVQLTAEAEAYRTVLVDGSKPHRPSDKEVDALTAQLEHTF
                     GQELDEQQKAGFAQFLTSEDGQVVVGQQRQVSDYIAEYDVTANPRYGQSFIVIARNAGP
                     QPLITSDIPS"
     gene            1897..2871
                     /locus_tag="SNAS_RS28755"
                     /old_locus_tag="Snas_5810"
     CDS             1897..2871
                     /EC_number="3.6.1.9"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF008989.0"
                     /locus_tag="SNAS_RS28755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_5810"
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="WP_013021011.1"
                     /transl_table=11
                     /translation="MTVTIHWLVTTPRLPAGMLTLQAWDALRASESVYAAPGNPQLGAV
                     RAAGIDVTELSVDDALDTLLERGGLWLTTAEDDEPVAKQLSVKLAADPSLATVEITFGS
                     WDPPGARLLDLVAVIDRLRSPGGCPWDREQTHESLAPFLLEEAYETIDAIHSGDLDHLR
                     EELGDLLMQPLLHARLAAEDEDGFTIDDVVADVVEKLVRRHPHVFADARVDSIADLNDT
                     WDALKRAEKPHRKFAVDGVALSQPALSLAAKLLSRTSRENLDVPLPETAADPASSEAAL
                     GAALLGLVAAARAAGLDPELALRKAALDFADRSRAVESGNKSG"
     gene            3109..3648
                     /gene="ectA"
                     /locus_tag="SNAS_RS28760"
                     /old_locus_tag="Snas_5811"
     CDS             3109..3648
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: protein motif:HMM:TIGR02406.1"
                     /locus_tag="SNAS_RS28760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_5811"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_013021012.1"
                     /transl_table=11
                     /translation="MSLGDTLAPVLRRNGGSAVGFEPPTIRDGERLWQLALDSRTLDVN
                     SRYSYLLWCRDFAETSVVARHDGEAVGFITGYRRPGSPGTLFIWQVAVADSHRRQGLAR
                     RMLDNLAARLIPQGITALEATVTPDNLPSTRLFTSFAEARGAELTRDELFSGQLLGEGH
                     LAEILFRIAPLSPASE"
     gene            3745..5004
                     /gene="ectB"
                     /locus_tag="SNAS_RS28765"
                     /old_locus_tag="Snas_5812"
     CDS             3745..5004
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (22-357). E-value: 4.5e-75.
                     Score: 244.7. Matches aSDomain:
                     nrpspksdomains_SNAS_RS28765_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084079.1"
                     /locus_tag="SNAS_RS28765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_5812"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_013021013.1"
                     /sec_met_domain="Aminotran_3 (E-value: 5.2e-76, bitscore:
                     254.5, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIFEEVESQVRSYCRGWPTVFVKAKDATLHDEDGKSYIDFFAGA
                     GTLNYGHNHPVLKQALIEYLTEDNIVHGLDMYTAAKGRFLDRFREIVLRPRGLDYKVQF
                     PGPSGNNAVEAALKLARKITGRETVISFTNGFHGMTLGALAVTGNSMKRGGAGVPLNHA
                     ATMPYDGYLDGQTPDFLWLRSLLEDSGSGLDKPAAVIVETVQGEGGINVASKTWLRGLA
                     DLCTEHDILLIVDDIQMGCGRTGPFFSFESAGITPDIVTISKSLSGYGLPFALTLFKKD
                     LDVWEPGEHNGTFRGFNPALVTAATALEHFWTDTKLAEDTLRKGELVDTRLRGIAAEHT
                     DVGSDVRGSGMVQALAFDDPKIAAATRAEAFSRGLLVEGAGPEDEVVKLLPPLTTTDAQ
                     LHEGLDVLADSVRVAIKELS"
     aSDomain        3811..4815
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SNAS_RS28765_Aminotran_3.1"
                     /evalue="4.50E-75"
                     /label="SNAS_RS28765_Aminotran_3.1"
                     /locus_tag="SNAS_RS28765"
                     /protein_end="357"
                     /protein_start="22"
                     /score="244.7"
                     /tool="antismash"
                     /translation="VKAKDATLHDEDGKSYIDFFAGAGTLNYGHNHPVLKQALIEYLTE
                     DNIVHGLDMYTAAKGRFLDRFREIVLRPRGLDYKVQFPGPSGNNAVEAALKLARKITGR
                     ETVISFTNGFHGMTLGALAVTGNSMKRGGAGVPLNHAATMPYDGYLDGQTPDFLWLRSL
                     LEDSGSGLDKPAAVIVETVQGEGGINVASKTWLRGLADLCTEHDILLIVDDIQMGCGRT
                     GPFFSFESAGITPDIVTISKSLSGYGLPFALTLFKKDLDVWEPGEHNGTFRGFNPALVT
                     AATALEHFWTDTKLAEDTLRKGELVDTRLRGIAAEHTDVGSDVRGSGMVQALAF"
     gene            5001..5420
                     /locus_tag="SNAS_RS28770"
                     /old_locus_tag="Snas_5813"
     CDS             5001..5420
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="SNAS_RS28770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_5813"
                     /product="ectoine synthase"
                     /protein_id="WP_013021014.1"
                     /sec_met_domain="ectoine_synt (E-value: 3e-53, bitscore:
                     177.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRRLNEIVGTERDVLGHDRDVAEGTWRSRRLILANDRVGFSMH
                     DTVLYAGTETSMWYANHVEAVYVIEGNGELIDNETGQKHELSPGTMYLLDGHERHTLRA
                     HTDVRTVCVFNPPVTGKEVHDANGVYPLLTEDDPQ"
     gene            5437..6327
                     /gene="thpD"
                     /locus_tag="SNAS_RS28775"
                     /old_locus_tag="Snas_5814"
     CDS             5437..6327
                     /EC_number="1.14.11.55"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:TIGR02408.1"
                     /locus_tag="SNAS_RS28775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_5814"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_013021015.1"
                     /sec_met_domain="PhyH (E-value: 5.2e-33, bitscore: 113.6,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIQDIYPTRSGRQPMMLYRTEDPVAYGTADDGPIDAETLEGYAD
                     NGFLTIDELLEPGELAEYQNELNRLLVDESLDGDERVVREKSSNDVRSIFEVHKISELF
                     GDLLTHDRVAGRARQILGSDVYIHQSRVNYKPGFNGNPFYWHSDFETWHAEDGMPRMRA
                     VSISIALTDNYAYNGGLMIMPGSHKTFVSCVGETPDDHYRESLKEQEIGTPDETSLSVL
                     AEKHGIELFTGPAGSATMFDCNCMHGSNGNITPFPRSNIFAVFNSVENTCVEPFAAKHP
                     RPPFIGSRDFTPVKR"
     gene            6407..7183
                     /locus_tag="SNAS_RS28780"
                     /old_locus_tag="Snas_5815"
     CDS             6407..7183
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1295: Sel1 domain
                     protein repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /locus_tag="SNAS_RS28780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_5815"
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_013021016.1"
                     /transl_table=11
                     /translation="MATLDAPYVLESLMDHTGPLREDFEAAREGDSNSMFNLGLAFARI
                     GQIDDAVHWYTRAAEHGDNDAVANLGLLLRDQERYDEAEKWLRHGAEAGHAMSANNLGN
                     LLLGWLRFDEALFWAERAVELGSTAAAGNLERVRLLARYHVPDTEPNREFARGMELVEK
                     ADVDAALPHLGAAAAQAHPDAAFELASLLAARDERDDAAGWYRIATMLGNPRAMVRLVM
                     VCLRQGNRDEARRWAEKAVESGETLAGPLLEALDED"
     gene            complement(7259..8539)
                     /gene="eno"
                     /locus_tag="SNAS_RS28785"
                     /old_locus_tag="Snas_5816"
     CDS             complement(7259..8539)
                     /EC_number="4.2.1.11"
                     /GO_function="GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="eno"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225678.1"
                     /locus_tag="SNAS_RS28785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_5816"
                     /product="phosphopyruvate hydratase"
                     /protein_id="WP_013021017.1"
                     /transl_table=11
                     /translation="MASIDNIAAREILDSRGNPTVEVEVLLDDGVLARAAVPSGASTGA
                     FEAIELRDKDEERYGGKGVEDAVQNVNERIVDALQGIDAADQRLVDQELLDLDGTSDKS
                     NLGANAILGVSLAVAKAAAASAGLPLFRYLGGPNAHVLPVPMLNILNGGSHADSNVDVQ
                     EFMIAPVGAPTFAEGLRWGAETYHALKSVLKSKGLSTGLGDEGGFAPNLEANSEALDLI
                     TEAVEKAGFAPGRDIVYALDVAATEFFADGSYTFEGEKKSAKDMIEYYANLIGEHPIVS
                     IEDPLSEDDWDAWSDMTAELGGKIQIVGDDLFVTNPERLRKGIDTAAATALLVKVNQIG
                     TLTETLDAVELAHRSGMRCMMSHRSGETEDTTIADLAVAVGCGQIKSGAPARSERVAKY
                     NQLLRIEEELDDTARYAGRTAFPKYRA"
     gene            complement(8631..10241)
                     /locus_tag="SNAS_RS28790"
                     /old_locus_tag="Snas_5817"
     CDS             complement(8631..10241)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /locus_tag="SNAS_RS28790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_5817"
                     /product="DUF885 domain-containing protein"
                     /protein_id="WP_013021018.1"
                     /transl_table=11
                     /translation="MHEEYVSRAERVVDALLESDPEAAMWAGDHRFDDRLPDYSSEGLA
                     AKRSMLTEASHALVEIDAEDLGTPDAVDLEILTNQVAARLFELTETRDHEWNPLVYNPG
                     MLLNTLLLRPSAPAAERLTALQARLAAIPDHLATARANLTDVPAVYAETGAEQFRGTAA
                     LVRDEVPRLAEEAGATVDVETVAGELEAFADWLSRLPDGRSPRLGRRLWDAKLWHTLET
                     PMAGASILDRAWTRLDEVTAELDELAARLDRDGGTADGASGAEVTARVLARLGADRPDN
                     ATVLDAARRAVAETTDFVREHDLITIPADRLEVVEMPEHDRGVSAAYLDPPGTLDDPSM
                     PTFYAICPAPADWSTEQVASFYSEYNHHMIRNLTVHEAIPGHHVQLAHARTYRGNTRVR
                     DMMMSGTFVEGWAVYTEELMVEHGYGGPEVLAQQLKMQLRMIINAIIDQSIHCDDMSEA
                     EAMDLMTRRGHQAHAEAASKWRRALLSSTQLSTYFVGYTEMRAIADSCPTGRPGRDWHD
                     RMLSFGSPAPQHVVRLLAG"
     misc_feature    complement(10252..10269)
                     /note="TFBS match to HipB, Bacterial antitoxin HipB,
                     confidence: weak, score: 20.94"
                     /tool="antismash"
     misc_feature    complement(10298..10311)
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.97"
                     /tool="antismash"
     misc_feature    complement(10305..10320)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.58"
                     /tool="antismash"
     misc_feature    complement(10345..10360)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.1"
                     /tool="antismash"
ORIGIN
        1 accggcatcc gcgagatgtc cacgatcacc accccgccgg aggagcggca cccggtgctg
       61 accttcgtgg gcggctacga ggacaagcag gtcggcgccg cgatccggcg cgagctgctg
      121 cgcgacggcc aggtgttcta cctgcacaac cgggtcgagt cgatcgagaa ggccgccacc
      181 cggctgcgcg agatcgtgcc ggaggcgcgg gtcgtggtcg cgcacggcaa gatgggcgag
      241 aaccggctgg aacagatcat gcaggacttc tggaacggcg agttcgacgt cctggtgtcc
      301 acgacgatca tcgagtcggg catcgacatc cccaacgcca acaccctgat cgtggagcgc
      361 gccgacctgc tgggtctgtc gcagctgcac cagatccggg gccgggtggg ccggggccgg
      421 gaacgcgcct acgcctactt catgtatccg ccggagaagc cgctgaccga gcaggcccac
      481 gagcggctgg cgaccgtcgc gcagcacacc gaactgggcg cgggcatgta cgtggccatg
      541 aaggacctgg agatccgggg cgcgggcaac ctcttgggcg gcgagcagtc cgggcacatc
      601 gcgggcgtcg gcttcgacct gtacgtgcgg atggtcggcg acgcggtgcg caccttcaag
      661 ggcgccaagg aggagatcct caccgaggtc aagatcgacc tgccggtgga cgcccacatc
      721 ccctacgact acatcccggt ggaacggctg cggctggaga tgtaccgcaa gctggccgag
      781 gtccgcgacg acgccgaact ggacgaggtc cgcaccgaga tggccgaccg ctacggcgat
      841 cttcccgccg aagtggacaa cctggtccac gtcgcccggt tccggctgct ggcccgcgcc
      901 gccggtctgt ccgatgtgtc gctccagggc cgccacatcc ggctgtcgtc gatcacgctg
      961 tcggactcca aacagctgcg cctcaagcgc cactaccccg actcggtgta caagaaggcc
     1021 gccgagctga tctcggtgcc gcgcccgcac accgcgcggg tcggcgggtc cccgatggag
     1081 ggcccggcac tgttgcagtg gtgcgccgaa ctcatcaagg acgtgaccga ggcaccagcc
     1141 ccggccgcta ctcctgcatg aattaaggtc tgggcggtac gaaaggactg aactcgatga
     1201 gaattcgcgc gatcgccgcg atgggcctgg ccgccgccgc actgctgacc gtgtccgcct
     1261 gccgggtcga acagggcgcc gcgctgttcg tcggcgacga gcggatcagc gagaagcggg
     1321 tcgacgaggt ggtcgacagc atctcggcct ccgaccgggc gaagctggcc gatctgaacc
     1381 agctcaccgg cgggcagctg ccggacatct cgacggggaa cctgcgcggc tacgtcgtgg
     1441 acgcgctggt ggtcaccgag ctgggtcagc gggtcgccgc cgacaccggt gtcaagccgg
     1501 accgcgagtc ctcgtcggtg atcaagaaga actgggagct gctggagctc gacagcgaca
     1561 gtgagttcgt ccagctgacc gccgaggccg aggcgtaccg caccgtgctg gtggacgggt
     1621 ccaagccgca ccgccccagc gacaaggagg tcgacgcgct gaccgcccag ctggagcaca
     1681 ccttcgggca ggagctcgac gagcagcaga aggccgggtt cgctcagttc ctcaccagtg
     1741 aggacggaca ggtcgtcgtc ggtcagcagc ggcaggtctc cgactacatc gccgagtacg
     1801 acgtgaccgc caacccgcgc tacgggcagt cgttcatcgt gatcgcccgc aacgccggac
     1861 cgcagccgct catcacctcc gacatcccca gctgacgtga ccgtcacgat tcactggctg
     1921 gtcaccacgc cacggctccc cgccgggatg ctgacgcttc aggcgtggga cgcgttgcgc
     1981 gccagtgagt ccgtgtacgc cgcgcccggg aacccgcagc tgggtgccgt gcgcgccgcc
     2041 ggtatcgacg tcaccgagtt gtccgtggac gacgccctcg acacgctgct ggagcgcggc
     2101 ggcctgtggc tgaccaccgc cgaggacgac gagccggtcg ccaagcagct gagcgtgaag
     2161 ctcgccgccg acccgtcgct ggccactgtg gagataacct tcggttcctg ggacccgccg
     2221 ggcgcccggc tgctggacct ggtggccgtc atcgaccggc tgcgctcgcc gggcggctgc
     2281 ccgtgggacc gggagcagac ccacgagtcg ctggcgccgt tcctgctgga ggaggcgtac
     2341 gagaccatcg acgccatcca ctccggcgac ctcgatcacc tgcgcgagga actgggcgac
     2401 ctgttgatgc agccgctgct gcacgcccgg ctggccgccg aggacgagga cggcttcacg
     2461 atcgacgacg tcgtcgccga cgtggtggag aagctggtgc gccgccaccc gcacgtgttc
     2521 gccgacgcgc gcgtcgacag catcgccgac ctcaacgaca cctgggatgc cctgaagcgg
     2581 gccgagaagc cgcaccgcaa gttcgccgtg gacggtgtcg cgctgtccca gcccgcgctg
     2641 tcgctggccg cgaaactcct gtcgcgcacc tcccgcgaga acctcgacgt gccactcccc
     2701 gagaccgccg cggatcccgc ttcctccgaa gcggcactgg gtgccgcgct gctgggtctg
     2761 gtcgccgccg cccgggcggc cggtctggac cctgaactgg ccctgcgcaa ggccgcgctc
     2821 gatttcgccg accgctcccg ggccgtcgaa tccgggaaca agtccgggta atctccgttc
     2881 gtttcccgca aatcttcccg ttttgccact gatttcgggg tagcggcgaa cagtttcacc
     2941 gtccatcacc ccagctcgtc cacaaattcg ccacctcttg tcggtcatcg acatgtctat
     3001 cgccctattg tcaacaattg acagggggat tttgacccaa tttgcctaga tatatccggg
     3061 caaattgggt cattattgga ggccattaag tcgcgctaag gttgatgcat gtccctcggt
     3121 gacactcttg ctcccgttct tcgacgaaac ggggggtcgg ccgtcggctt tgaacccccc
     3181 acgatccgcg atggcgagcg gctgtggcaa ctggccctcg actcccgcac tctcgatgtg
     3241 aactcccgct acagctacct gctgtggtgc cgcgacttcg ccgaaacctc ggtggtggcg
     3301 cggcacgacg gcgaggcggt gggtttcatc acggggtacc ggcgtcccgg ctcccccgga
     3361 accctgttca tctggcaggt ggcggtggcc gactcgcacc gccgacaggg acttgcccgg
     3421 cgcatgctgg acaacctcgc cgcgcggctg atcccgcagg ggatcaccgc gctcgaggcg
     3481 accgtcacgc cggacaacct tccgtccacg cgtctgttca cctccttcgc cgaagcgcgg
     3541 ggagccgaac tgacccggga cgagctgttc tccgggcaac tgctcggcga ggggcacctg
     3601 gcggagatcc tgttccggat cgcgccgctg tcacccgcgt ccgaatagga ccccgtttcg
     3661 ggaccctagc cgatcccgcg cggacccaca tctcctgatg caccgcgacc gagacagcag
     3721 tcatcggccg acgaaggaat acctatgaag atattcgaag aagtcgaatc acaagttcgc
     3781 tcgtactgcc gcggctggcc gacagtcttc gtcaaggcca aggacgccac gctccacgac
     3841 gaagacggaa agtcctatat agactttttc gccggtgcgg gaaccctcaa ctacgggcac
     3901 aaccaccccg tcctcaaaca ggcgctcatc gagtacctga ccgaggacaa catcgtgcac
     3961 ggcctcgaca tgtacaccgc cgccaagggc cggttcctcg accggttccg cgagatcgtg
     4021 ctgcggccgc gcggcctgga ctacaaggtc cagttcccgg ggccgtcggg caacaacgcc
     4081 gtcgaggcgg cgctgaaact ggcccgcaag atcaccggcc gcgagaccgt catcagcttc
     4141 accaacggtt tccacggcat gaccctcggc gcgctggcgg tcaccggcaa ctccatgaaa
     4201 cgcggcggcg ccggggttcc gctcaaccac gccgccacca tgccgtacga cggctatctg
     4261 gacggtcaga caccggactt cctgtggctg cgcagtctcc tggaggacag cggttccggg
     4321 ctcgacaagc ccgccgcggt catcgtggag acggtgcagg gcgagggcgg catcaacgtc
     4381 gccagcaaga cctggctgcg gggtctggcc gacctgtgca ccgagcacga catcctgctc
     4441 atcgtggacg acatccagat gggttgcggc cgtaccggac cgttcttcag tttcgagtcc
     4501 gccgggatca cgcccgacat cgtgacgatc tccaaatcgc tgtccggcta cggactcccg
     4561 ttcgcgctca ccctgttcaa gaaggacctg gacgtgtggg agcccggcga gcacaacggc
     4621 accttccgcg gtttcaaccc ggcactcgtc accgcggcca cggcgctgga gcacttctgg
     4681 accgacacga agctggccga ggacacgctc cgcaagggcg agctcgtcga cacccggctg
     4741 cgcggcatcg ccgccgagca caccgacgtc ggcagcgacg tgcgcggctc cggaatggtg
     4801 caggcgctgg ccttcgacga ccccaaaatc gccgcggcca cccgtgccga ggccttcagc
     4861 cgcggcctgc tggtcgaggg tgcgggcccc gaggacgagg tcgtcaaact gttgccgccg
     4921 ttgacgacga cggacgcgca gctgcacgag ggcctcgacg tcctcgccga ttccgtccga
     4981 gtggccatca aggagttgtc atgatcgttc gcaggctcaa cgagatcgtc ggtaccgagc
     5041 gggatgtgct cggccacgac cgcgacgtcg ccgaggggac ctggcggtcc cgccgtctca
     5101 tcctcgccaa cgaccgggtc ggtttctcga tgcacgacac cgttctgtac gcgggcaccg
     5161 agacctccat gtggtacgcc aaccacgtcg aggccgtcta cgtcatcgag ggcaacggcg
     5221 aactcatcga caacgagacc ggtcagaagc acgagctctc gccgggaacc atgtacctgc
     5281 tcgacggaca cgaacgccac accctgcggg ctcacaccga cgtgcgcacc gtctgcgtct
     5341 tcaacccacc ggtcaccggc aaggaagtcc acgacgccaa cggcgtctat ccacttctga
     5401 cagaagacga cccccaatag aacggaggca gttaaagtga ccattcagga tatctacccc
     5461 acccgttccg gccgccagcc catgatgctg taccgcaccg aggacccggt cgcctacggc
     5521 accgccgacg acggcccgat cgacgccgag accctggaag gctacgccga caacgggttc
     5581 ctcacgatcg acgagctact cgagcccggc gaactggccg agtaccaaaa cgagctcaac
     5641 cggctgctgg tggacgagag cctcgacggc gacgagcgtg tcgtgcgcga gaagtcctcc
     5701 aacgacgtgc gttccatctt cgaggtgcac aagatctccg aactgttcgg cgacctgctc
     5761 acccacgacc gggtcgcggg ccgggcccgg cagatcctcg gctccgacgt ctacatccac
     5821 cagagccgcg tcaactacaa gcccggtttc aacggcaacc cgttctactg gcactccgac
     5881 ttcgagacct ggcacgccga ggacggaatg ccccggatgc gggccgtcag catctccatc
     5941 gcgctgaccg acaactacgc ctacaacggc ggtctgatga tcatgccggg ctcccacaag
     6001 accttcgtgt cctgtgtggg cgagacgccg gacgaccact accgcgaatc gttgaaggaa
     6061 caggagatcg gtacccccga cgagacctcg ctgtcggtcc tcgccgagaa gcacggcatc
     6121 gagctgttca ccggccccgc cggttcggcg accatgttcg actgcaactg catgcacggt
     6181 tccaacggca acatcacccc gttcccgcgc tccaacatct tcgcggtgtt caacagcgtc
     6241 gagaacacct gtgtcgaacc gttcgcggcc aagcacccgc gtccgccgtt catcggctcg
     6301 cgggacttca cgccagtgaa gcgttagcac agcacgccag tgaagcgtta gaaacctcgc
     6361 accagaagcg aaggccccga atcctctcgg attcggggcc ttcgtcatgg ctaccctgga
     6421 cgccccctac gtattggagt ccctcatgga tcacaccggc cccctgcgcg aggacttcga
     6481 ggcggcgcgc gagggtgact ccaactcgat gttcaacctc ggtctggcct tcgcccggat
     6541 cgggcagatc gacgacgccg tgcactggta cacccgcgcc gccgagcacg gtgacaacga
     6601 cgccgtcgcg aacctcgggt tgctgctgcg ggaccaggaa cggtatgacg aggccgagaa
     6661 gtggctgagg cacggcgccg aggccgggca tgccatgtcg gccaacaatc tcggcaacct
     6721 gctgctgggc tggctgcgct tcgacgaggc actgttctgg gccgagcgcg cggtggaact
     6781 gggcagcacc gctgcggccg ggaacctgga gcgggtgcga ctgctcgcca ggtaccacgt
     6841 cccggacacc gaaccgaacc gggagttcgc acgcggcatg gaactggtgg agaaggccga
     6901 tgtggacgcc gcgctgccgc acctgggtgc cgcggcggca caggctcacc cggacgcggc
     6961 cttcgaactc gcgtcgctgc tggccgcccg cgacgaacgg gacgacgccg cgggctggta
     7021 ccgcatcgcg acgatgctcg gcaacccccg tgcgatggtg cggctcgtga tggtctgtct
     7081 gcgacagggc aatcgcgacg aagcccggcg atgggcggag aaggcggtgg agtccggtga
     7141 aaccctcgcg ggcccgctgc tcgaagccct cgacgaggac tgagacccgg cctccggtcg
     7201 agacgcggac gggccgccac gctttcggcg tgacggcccg tcgagctggt ccggaggatc
     7261 aggcgcggta cttcgggaag gcggtgcgac ccgcgtaacg ggcggtgtcg tccagctcct
     7321 cctcgatgcg cagcagctgg ttgtacttgg cgacccgctc ggagcgggcg ggggcgccgg
     7381 acttgatctg tccacagccg acggccacgg ccaggtcggc gatggtggtg tcctcggtct
     7441 cgccggagcg gtggctcatc atgcagcgca taccgctgcg gtgcgccagt tccacggcgt
     7501 ccagcgtctc ggtcagggtg ccgatctggt tgaccttgac caacagggcg gtggccgcgg
     7561 cggtgtcgat ccccttgcgc agccgctcgg ggttggtgac gaacaggtcg tcgccgacga
     7621 tctggatctt gccgcccaac tcggcggtca tgtccgacca ggcgtcccag tcgtcctcgg
     7681 acagcgggtc ctcaatggag acgatcgggt gctcgccgat gaggttggcg tagtactcga
     7741 tcatgtcctt ggcggacttc ttctcgccct cgaaggtgta gctgccgtcg gcgaagaact
     7801 cggtggcggc cacgtcgagc gcgtagacga tgtcgcggcc cggcgcgaag cccgccttct
     7861 cgacggcctc ggtgatgagg tccagggcct cgctgttggc ctccaggttg ggggcgaagc
     7921 cgccctcgtc gcccaggccg gtggacaggc ccttggactt cagcaccgac ttcagcgcgt
     7981 ggtaggtctc ggctccccag cgcagcccct cggcgaaggt gggggcgccg acgggggcga
     8041 tcatgaactc ctggacgtcc acgttggagt ccgcgtgcga gccgccgttg aggatgttga
     8101 gcatcggcac cggcagcacg tgcgcgttgg ggccgcccag gtagcggaac agcggcagac
     8161 cggccgaggc ggcggcggcc ttggcgacgg ccagcgagac gccgaggatg gcgttggcgc
     8221 ccaggttgga cttgtcggag gtgccgtcca ggtcgagcag ttcctggtcg accaggcgct
     8281 ggtcggcggc gtcgatgccc tgcaacgcgt cgacgatgcg ctcgttgacg ttctggaccg
     8341 cgtcctcgac gcccttgccg ccgtagcgct cctcgtcctt gtcgcgcagc tcgatcgcct
     8401 cgaaggcacc ggtcgaggcg cccgagggca ccgcggcccg cgcgaggacg ccgtcgtcga
     8461 gcagcacctc gacttcgacc gtcgggttgc cgcgcgaatc caggatttcc cgggcggcga
     8521 tgttatcgat gcttgccact gctcgcactc cttatattcg agtcttggga tcacacgcca
     8581 gcgcggcgct gcgcttcgac tgtgttcgcc aagaacccta gaccgcgtcg ctatccggcc
     8641 agcagcctga ccacgtgctg tggtgccggg ctcccgaagg acagcatccg gtcgtgccag
     8701 tcgcgccccg gtcggccggt cgggcagctg tcggcgatgg cgcgcatctc ggtgtagccg
     8761 acgaagtacg tggacagctg ggtggacgac agcagcgcgc ggcgccactt gctcgcggcc
     8821 tcggcgtggg cctggtggcc gcgccgggtc atcaggtcca tggcctcggc ctcggacatg
     8881 tcgtcacagt ggatcgactg gtcgatgatg gcgttgatga tcatgcgcag ctgcatcttc
     8941 agctgctggg ccagcacctc cgggccgccg tagccgtgct cgaccatcag ctcctcggtg
     9001 tagaccgccc agccctccac gaaggtcccg ctcatcatca tgtcccgcac ccgggtgttg
     9061 ccccggtagg tgcgggcgtg ggcgagctgg acgtggtggc ccgggatcgc ctcgtggacg
     9121 gtgaggttgc ggatcatgtg gtggttgtac tcggagtaga acgacgcgac ctgttcggtg
     9181 ctccagtcgg ccggggccgg gcagatcgcg tagaaggtcg gcatcgacgg gtcgtccagg
     9241 gtgcctggcg ggtcgaggta cgcggccgac acgccccggt cgtgttcggg catctccacg
     9301 acctcgagcc ggtcggcggg gatggtgatc aggtcgtgtt cgcgcacgaa gtcggtggtc
     9361 tcggcgaccg cgcggcgggc ggcgtccagg acggtggcgt tgtcggggcg gtcggcgccc
     9421 agccgggcca gcacccgggc ggtgacctcg gcaccggacg cgccgtcggc cgttccgccg
     9481 tcgcggtcga gtcgcgccgc cagttcgtcg agctcggcgg tgacctcgtc cagccgggtc
     9541 caggcccggt ccagaatcga cgctcccgcc atcggcgtct ccagggtgtg ccacagcttg
     9601 gcgtcccaca gtcgacggcc cagtcgcggc gagcgtccgt ccggcagccg cgacagccag
     9661 tcggcgaacg cctccagctc accggcgacg gtctcgacgt cgacggtcgc accggcctct
     9721 tcggccagtc gcggcacctc gtcgcgcacc agcgcggcgg tgccccggaa ctgttcggcg
     9781 ccggtctcgg cgtagacggc gggcacgtcg gtcaggttgg cgcgcgcggt ggccaggtgg
     9841 tcggggatgg ccgccagccg cgcctgaagt gccgtcagtc gttcggcagc cggggccgag
     9901 ggccgcagca gcagcgtgtt gagcagcatg ccggggttgt agaccagcgg gttccactcg
     9961 tggtcgcggg tctcggtcag ctcgaacagc cgcgcggcga cctggttggt gaggatctcc
    10021 aggtcgacgg cgtcgggggt gcccaggtcc tcggcgtcga tctcgaccag cgcgtgcgag
    10081 gcctcggtca gcatcgaccg cttggcggcc aggccctcgg acgagtagtc cggcagccgg
    10141 tcgtcgaagc ggtggtctcc cgcccacatc gccgcctccg ggtcggattc cagcagcgcg
    10201 tcgacgacgc gttcggcccg ggatacgtat tcctcgtgca tgcccacacc gtaaccgccc
    10261 gaggggagac ggtttccacc gcgttttcgg ccgtcagttg atccgtcaca aaaccctgac
    10321 ggttcgaccg atgtgcacat gggcaatact caatatcgtt cagcctgccc cagggcgaac
    10381 acggagcacc ggcacggttc cgtcagtagg aaagatccac
//
