LOCUS       NC_013947              10354 bp    DNA     circular CON 23-APR-2024
DEFINITION  Stackebrandtia nassauensis DSM 44728, complete sequence.
ACCESSION   NC_013947
VERSION     NC_013947.1
KEYWORDS    RefSeq.
SOURCE      Stackebrandtia nassauensis DSM 44728
  ORGANISM  Stackebrandtia nassauensis DSM 44728
            Bacteria; Actinomycetota; Actinomycetes; Glycomycetales;
            Glycomycetaceae; Stackebrandtia.
REFERENCE   1  (bases 1 to 6841557)
  AUTHORS   Munk,C., Lapidus,A., Copeland,A., Jando,M., Mayilraj,S., Glavina Del
            Rio,T., Nolan,M., Chen,F., Lucas,S., Tice,H., Cheng,J.F., Han,C.,
            Detter,J.C., Bruce,D., Goodwin,L., Chain,P., Pitluck,S., Goker,M.,
            Ovchinikova,G., Pati,A., Ivanova,N., Mavromatis,K., Chen,A.,
            Palaniappan,K., Land,M., Hauser,L., Chang,Y.J., Jeffries,C.D.,
            Bristow,J., Eisen,J.A., Markowitz,V., Hugenholtz,P., Kyrpides,N.C.
            and Klenk,H.P.
  TITLE     Complete genome sequence of Stackebrandtia nassauensis type strain
            (LLR-40K-21)
  JOURNAL   Stand Genomic Sci 1 (3), 234-241 (2009)
   PUBMED   21304662
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6841557)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
            Mavromatis,K., Ivanova,N., Ovchinnikova,G., Munk,A.C., Brettin,T.,
            Detter,J.C., Han,C., Tapia,R., Larimer,F., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,M.,
            Mayilraj,S., Goker,M., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     The complete genome of Stackebrandtia nassauensis DSM 44728
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 6841557)
  AUTHORS   Lucas,S., Copeland,A., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Bruce,D., Goodwin,L., Pitluck,S., Kyrpides,N.,
            Mavromatis,K., Ivanova,N., Ovchinnikova,G., Munk,A.C., Brettin,T.,
            Detter,J.C., Han,C., Tapia,R., Larimer,F., Land,M., Hauser,L.,
            Markowitz,V., Cheng,J.-F., Hugenholtz,P., Woyke,T., Wu,D., Jando,M.,
            Mayilraj,S., Goker,M., Klenk,H.-P. and Eisen,J.A.
  CONSRTM   US DOE Joint Genome Institute (JGI-PGF)
  TITLE     Direct Submission
  JOURNAL   Submitted (01-OCT-2009) US DOE Joint Genome Institute, 2800 Mitchell
            Drive, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000024545.1-RS_2024_04_23
            Annotation Date                   :: 04/23/2024 01:07:54
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,519
            CDSs (total)                      :: 6,464
            Genes (coding)                    :: 6,414
            CDSs (with protein)               :: 6,414
            Genes (RNA)                       :: 55
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 50
            CDSs (without protein)            :: 50
            Pseudo Genes (ambiguous residues) :: 0 of 50
            Pseudo Genes (frameshifted)       :: 0 of 50
            Pseudo Genes (incomplete)         :: 50 of 50
            Pseudo Genes (internal stop)      :: 0 of 50
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##Metadata-START##
            Organism Display Name :: Stackebrandtia nassauensis LLR-40K-21, DSM
            44728
            Culture Collection ID             :: DSM 44728
            GOLD Stamp ID                     :: Gc01107
            Funding Program                   :: DOE-GEBA 2007
            Number of Reads :: 81931 Sanger, 851638 pyrosequence
            Assembly Method :: Newbler version 1.1.02.15, Phrap
            Sequencing Depth                  :: 11x Sanger; 29x 454
            Gene Calling Method               :: Prodigal 1.4, GenePRIMP
            Isolation Site                    :: Soil sample from Nassau Bahamas
            Isolation Country                 :: Bahamas
            Latitude                          :: 25.066127
            Longitude                         :: -77.339025
            Oxygen Requirement                :: Aerobe
            Motility                          :: Nonmotile
            Sporulation                       :: Nonsporulating
            Temperature Range                 :: Mesophile
            Temperature Optimum               :: 28C
            Gram Staining                     :: gram+
            Biotic Relationship               :: Free living
            Diseases                          :: None
            Habitat                           :: Soil
            ##Metadata-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 17:48:03
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 3862488
            Orig. end                         :: 3872842
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP001778.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4082381
            Source DNA and organism available from Hans-Peter Klenk at the
            German Collection of Microorganisms and Cell Cultures (DSMZ)
            (hans-peter.klenk@dsmz.de)
            Contacts: Jonathan A. Eisen (jaeisen@ucdavis.edu)
            David Bruce (microbe@cuba.jgi-psf.org)
            Whole genome sequencing and draft assembly at JGI-PGF
            Finishing done by JGI-LANL
            Annotation by JGI-ORNL and JGI-PGF
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10354
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="12"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10354
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10354
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5354](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5354)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            281..1918
                     /locus_tag="SNAS_RS17895"
                     /old_locus_tag="Snas_3629"
     CDS             281..1918
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SNAS_RS17895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Snas_3629"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_013018860.1"
                     /transl_table=11
                     /translation="MGTGKRGRPTQPTVKRHIRGGLGCEYRIAWMLRINRLYGDDQSLS
                     VGKKFARAFRGGSWDREVDGSQITHWEKLDQIPGYEVIRRYEELLGLPRNSLIAVADVV
                     YHDKRGTPGSPHLKRPPDPYGIRRAGLGDVVDRALSTDAMNGDDWDALTANLDATRTVL
                     PASDPGWPAVVERLAQELLLAEHIHWAQRNEAMVRFVGDPSSTSMVIAVCDALSADSRN
                     QVSSELLGLLEITPHPDAMAHLIRQVSTPVNEVARRGAWWAAAEKTGRGHLRTPDLIAL
                     AKHARRLLAGYEGHPDCRSAAANVLYQLHAVTPALFKSLRLNESDASVRNVLTGGRIVA
                     VTDASVTVNRIAEVVLGQMSSGHLSTDPILAELLGDALFDPQVTARLGALSLIRVTPYR
                     KPTANVLAMELGGKDARSNPDLFASMVRALAFLGTPTHRQHLEKIALATDGFAPRFRAT
                     VIHSLAHCGESDSADEVWTALIVRHLMNQSNPMEKHIADGLIYALGIQRRFGSLRRLVA
                     NPAMPGPVRAAASWWLNLPEHVLRSTSR"
     gene            complement(1922..2449)
                     /locus_tag="SNAS_RS17900"
                     /old_locus_tag="Snas_3630"
     CDS             complement(1922..2449)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SNAS_RS17900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Snas_3630"
                     /product="hypothetical protein"
                     /protein_id="WP_144300555.1"
                     /transl_table=11
                     /translation="MTAFVITNTSGIAIGLFCSVLWLWLLRRIRPRIKISSKIAEKVDP
                     ELGPSRFYIKISNLRRRAAVDLQIELVTLHLKQASNGEIYAKTMIPIQNALPFVLPGRD
                     ADCIYGHVLRLAIRHDLRAELANGEATIVQLRIYARDELSGMGRVFEKRYTDPETDLIQ
                     GAFSPGETYEIH"
     gene            complement(2455..2961)
                     /locus_tag="SNAS_RS17905"
                     /old_locus_tag="Snas_3631"
     CDS             complement(2455..2961)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SNAS_RS17905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Snas_3631"
                     /product="hypothetical protein"
                     /protein_id="WP_013018862.1"
                     /transl_table=11
                     /translation="MNVDIAILGVLIIAAVTTYFLRGKKPRRILDELCKEDTIATSLRL
                     SQVRGIVLRALRHPLGALVVENPNHFECSFSHRVRGVAYTVALRIDLHVRSTHRANGVW
                     VRVGVVEMDFPGTMTDPAFRAAFGDVVVLRKRVIRALATADITSTRSTVNITWAHRTHC
                     VRAGW"
     gene            complement(3198..3689)
                     /locus_tag="SNAS_RS17910"
                     /old_locus_tag="Snas_3632"
     CDS             complement(3198..3689)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SNAS_RS17910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Snas_3632"
                     /product="hypothetical protein"
                     /protein_id="WP_041625024.1"
                     /transl_table=11
                     /translation="MATPTPGFRTVDRLGPGTRGYTLPPEQPPGSPPPETGFARLPSFG
                     NRKRLLPLLAGLVALFVITSAVFIILFFHTRSDLTAAKATITKHEATINDADKKIKKLG
                     GSNDDLEDDVEEAEADSKAARECIDAFQAWLDEATKKKPDKSTVKDLADKANTACREFW
                     "
     misc_feature    complement(3842..3853)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: strong, score: 20.58"
                     /tool="antismash"
     gene            4330..4473
                     /locus_tag="SNAS_RS35840"
                     /old_locus_tag="Snas_3634"
     CDS             4330..4473
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="SNAS_RS35840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Snas_3634"
                     /product="hypothetical protein"
                     /protein_id="WP_013018864.1"
                     /transl_table=11
                     /translation="MEHNAQRIAIAAAAQARGLIQRSLTHDVPIDARLLTESCAALATL
                     SQ"
     gene            complement(5001..5354)
                     /locus_tag="SNAS_RS36780"
                     /old_locus_tag="Snas_3636"
     CDS             complement(5001..5354)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Lactococcin_972"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF021219.4"
                     /locus_tag="SNAS_RS36780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_3636"
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_041625025.1"
                     /sec_met_domain="Lactococcin_972 (E-value: 1.5e-08,
                     bitscore: 33.7, seeds: 6, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTLVAKGAAVATMVIMMMLGGASAAQASPGGGTSNSGDVTVQSC
                     SGVGGGVWCRGYVNLSNGNKRCYSDYRHDTKVHGARVAGGNKRAETWQNPGIWAKTSIE
                     VSRSVACSYAALV"
     gene            5669..7942
                     /locus_tag="SNAS_RS17920"
                     /old_locus_tag="Snas_3637"
     CDS             5669..7942
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /locus_tag="SNAS_RS17920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_3637"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_013018866.1"
                     /transl_table=11
                     /translation="MNANGFTIGCGRLLRAHRLRAGLAQEDLARISGVSVRGIRMIENE
                     QRRPRVSTVRTVAQSLRLSEADRLTLMEAAQPDSRPPQKPENHPRRVVPRQLPASPRGF
                     VAREAELGRLDAALKPPTSLLCSVTGLGGMGKTWLAVHWATTRLDRFPDGQLYVNLRGF
                     DPVTEPTPPYVGLRAMVSALGVPVDSVPADVDELAALFRSITADKRLLMVLDNARDAAQ
                     VAPMLPGGDGSAAVVTSRSSLCELVTEYGARSLRLGGLTADDSRRLLGEQWDAERIARE
                     PDAVEGLISRCAGLPLALGIVAAHGVLNPDHPLGTIYRNLVDDSANSAEDGDDIVDDIR
                     VVFDTSLRLVGGDASRLFDLLGLASGSGIDVYAAASLAGLPVPAARGALRELENAHLVS
                     RDSPGRYRMHDLTYRFAAARSSAVIDEPETVAALHRLINHYILTGYRAGDAMQQGDAPV
                     TENIGLTPRCVITPMADRAAAFDWVRREIEDFPRYQAIALAHGWYETAWRVADCTRLFI
                     DEDAINAVAEQELWRVAIDAAEHLGPMHLARAQSRFGNLRLKTDPSDSEAVDLMLKAAD
                     LAAASGEVAECARTHFMLAHRFQHNGQKAQAIVHAKLALAFLTETEEPVMIARVHNMIS
                     WTLGSAGRFHDARRHGELALAIAREYGLSHTEATVLGTLGSNSHKAGRHAQAERLLRRA
                     YTIHSTMAQNPWSAISLENLGDVLADSRHPDQASEAWRRATILFEQTFRIKDAERVRTK
                     LDSL"
     gene            complement(7962..8822)
                     /locus_tag="SNAS_RS17925"
                     /old_locus_tag="Snas_3638"
     CDS             complement(7962..8822)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1170: metallo-beta-lactamase family protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /locus_tag="SNAS_RS17925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_3638"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_013018867.1"
                     /transl_table=11
                     /translation="MIDFDRGAPVAGDLDVAWHAGSPSAKHDTADEIQSHHYSEHTVIM
                     RQNKAVHYEAPFMFLLFGNDRAVLIDTGATPKERFFPLRATVDAHIDRWLERHPRPDYG
                     LLVAHTHGHGDHKAADGQFTDRPNTVVVGTDLDEVVEFYGFTDWPDTSRTLDLGGRRLE
                     VIPGPGHHPSATVFYDPHTGLLLTGDTLYPGRLYVDDWQDFGTTFTKLREFCANHPVTH
                     VLGCHIEMSRTPSEDYPIRTVYQPEEPPLQLSVSDVNDVCEAIEAIGDSPGIHRYDRFI
                     IHRGH"
     gene            complement(8843..9490)
                     /locus_tag="SNAS_RS17930"
                     /old_locus_tag="Snas_3639"
     CDS             complement(8843..9490)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017808.1"
                     /locus_tag="SNAS_RS17930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Snas_3639"
                     /product="response regulator transcription factor"
                     /protein_id="WP_013018868.1"
                     /transl_table=11
                     /translation="MSIRVLIADDQPIVRTGLTMLLDAQPDIEVVGAAADGREAVRLAL
                     QLRPDVGLFDIRMPLMDGIEATRRLAGPDVTDPLPIVVITTFDLDEYVHEALKAGARGF
                     LLKDAGPALLTQAIHAAADGNALIAPSVTVRLIAAFAQTHTSPPPQPIERLTAREEEVL
                     VSVAQGRTNNEIASELYITQSTVKAHLASLMRKLGARNRVEVAMWAHETGRV"
ORIGIN
        1 ttctttcgtg cccagctggc aatggcggcg attattagaa cgaagatgac gaaaccgaac
       61 acgttcgccg gatccatatc ggtggctcct ccagtggttg attgaatcac cgccaagatt
      121 gcgccagctt cacgtggaaa ccaccgattt attgcagaga tcaagccggg taaccattaa
      181 ggacgcttct ggtgccgatg cggcctactt tggttgcggg ggaacaagac caggaccttt
      241 gactcataca tgtttgccat cggattcgta tgttgtggtt atgggaacgg gtaagcgcgg
      301 ccgtccaacc cagccaaccg tcaaacgcca catccgtggt ggcttgggtt gcgagtaccg
      361 catcgcctgg atgttgagaa tcaatcgtct atacggtgac gaccagtctt tgtcggtggg
      421 caaaaagttc gcgcgagcgt tccgtggcgg cagttgggac cgggaagtag acggttcgca
      481 gatcacacac tgggagaaac tcgaccagat accgggttac gaggtgatcc gccggtacga
      541 ggaactgctc ggtctgcccc gtaactctct catcgccgtc gccgatgtcg tctatcacga
      601 caaacgaggc actcccggta gtccgcacct gaagcgaccg cccgacccct acggcattcg
      661 ccgcgccggg ctcggtgacg tggtggaccg ggcgttgagc actgacgcga tgaacggcga
      721 cgactgggat gccctgaccg cgaacctgga cgcgactcga actgttctac ccgcttcgga
      781 cccgggatgg cccgcagtcg tcgaacggct ggcccaggaa ctgttgctgg ccgagcacat
      841 ccattgggcg caacgcaatg aggcgatggt gcggttcgtc ggagacccct ccagcacgtc
      901 tatggtcatc gcggtgtgtg acgcactttc ggcggattcg cgcaaccagg tgtcgagcga
      961 gctgttgggg ttgttggaga ttacgccgca tccggacgcc atggctcacc tgatccgtca
     1021 agtgtccaca cccgtcaacg aagtagcccg gcgtggtgcg tggtgggcgg cggctgagaa
     1081 gactggtcgc ggccacttgc ggacaccgga tctgatcgcg ttggccaaac atgcgagaag
     1141 actgctcgcg ggctatgaag gccatcccga ctgccgttca gcggcggcca acgttttgta
     1201 ccagcttcac gcggttacac cggctctgtt caagtccttg cggctgaatg aaagcgacgc
     1261 ctcagtccga aatgtactca ccggcggccg catcgttgcg gtgaccgacg cgtccgtgac
     1321 ggtgaaccgg atagcggagg tggtacttgg ccaaatgtca tccggtcacc tgtccactga
     1381 cccgatattg gccgaactcc tgggggatgc tctgttcgat cctcaggtca ccgcacggct
     1441 cggcgcgttg tcgctgatta gagtgacccc gtaccggaaa ccgacggcga acgtgctggc
     1501 gatggagctg ggtggaaaag atgctcgctc caatccggac ctctttgcca gtatggtgcg
     1561 cgccttggca ttcctgggca cgccgacgca ccggcagcat cttgagaaga tcgcgctcgc
     1621 caccgatggc ttcgcgcctc ggttccgagc cacggtgatc cactcgctgg ctcactgcgg
     1681 cgaaagtgac agtgccgacg aggtctggac ggcactcata gtgcggcacc tgatgaacca
     1741 gagcaatccg atggaaaagc acatcgctga cgggttgatc tacgcgctgg gaattcagcg
     1801 ccgcttcggc agtctgcgga ggcttgttgc caatcccgcc atgcccggac cggtgcgagc
     1861 cgccgcgtcc tggtggttga acctgcccga gcatgttttg cgtagcacct cgcgttgagg
     1921 ctcaatggat ctcgtaggtc tctcctggac tgaacgcgcc ttggatgagg tcggtttccg
     1981 ggtcggtata acgcttttcg aagactctgc ccatgccaga cagttcatct ctggcgtaga
     2041 ttcgcagttg aacgatcgtt gcttcgccat tggcgagttc ggcacgcaga tcgtggcgga
     2101 tcgccagcct tagtacgtgt ccgtaaatac aatcggcgtc tcgaccgggc aacacgaacg
     2161 gcagtgcgtt ctgtattggg atcatggtct tggcgtagat ttcaccgttg ctggcctgct
     2221 taaggtgcaa ggtcacgagc tcgatctgta gatcgaccgc ggctcgacga cgcagatttg
     2281 agatcttgat gtagaaccgg ctggggccga gttcgggatc cactttctcc gcgatcttgc
     2341 tggagatctt gatccggggc cgaatgcggc gcaacagcca cagccaaagc acggagcaga
     2401 acaacccgat cgcgattcca ctggtattgg taatgacgaa ggcggtcatg atcattacca
     2461 cccggcccgt acacagtggg tccgatgcgc ccacgtgatg tttaccgttg atctagtgga
     2521 ggtgatgtcc gccgtggcaa gcgcacgaat cacccgcttg cgcagcacca cgacatcgcc
     2581 gaatgccgcg cgaaacgccg gatcagtcat cgtcccggga aagtccatct ccacgacacc
     2641 tactcgcacc caaactccgt tggctcggtg tgtgctgcga acgtgtaggt cgatgcgaag
     2701 cgcgactgta tacgcgacgc ctcgtacacg gtggctgaag gaacattcaa agtggttcgg
     2761 gttctcgacg accaaggctc ccagcggatg gcgcagtgcg cggaggacga tcccccggac
     2821 ttgggacagc ctcagcgatg tggcgatcgt atcctcttta cacagttcgt cgagtatccg
     2881 tctcggtttc ttgcctcgta ggaaataggt cgtgactgcg gcgattatga gcactcccaa
     2941 aatcgcgatg tcaacattca tgactctggc tcctttgttg aactggatga gtcaaagcca
     3001 aggttgcgcc aggaaagccg gaaagctatg gaataattgt ggagatcatt aaacgtcaac
     3061 cactatggac tattgccata tcgcgctccc ggagtcggcc gcgatggcgg tgggggacct
     3121 tgatttcgtc agcgaccatc ggaccctcgt ccagtccgca gacaaacctc atcgcaccag
     3181 gcacctgtcg cgggtgttca ccaaaattcg cggcaggcgg tgttggcttt gtcggccagg
     3241 tccttcacgg tgctcttgtc gggtttcttc ttggtggctt cgtccagcca cgcctgaaac
     3301 gcgtcgatgc attcgcgggc ggccttgctg tcggcttcgg cttcttcaac atcgtcttcg
     3361 aggtcgtcgt tgctgccgcc cagtttcttg atcttcttgt cagcgtcgtt gatggtggct
     3421 tcgtgtttgg tgatcgtcgc tttggcggcg gtcaagtcgc ttcgggtgtg gaagaacaag
     3481 atgatgaaca ctgccgaagt gatgacgaac agcgcgacca gcccggccaa cagcggcagc
     3541 agccgtttgc gattcccgaa ggatggcagg cgggcgaacc cggtttcggg tggcggtgaa
     3601 cctggcggct gttccggcgg gagggtgtat ccgcgggtgc ccgggccaag gcgatcgacg
     3661 gtccggaagc ctggcgtcgg ggtggccatg ggtggcgggt atggcggagt ccccgatgga
     3721 ccggtgcccg gggatgatgg cacgttggtt ggcgggtagt ggtggttgga gtgttggggg
     3781 tcgtgcatgc ggatcctcct cgacagaaca cggcaccgcc tggtgccacc tcgaggctgg
     3841 agcgttcgcg tacacgcgat gtacatggcg gcagtgaccc cgccgacacc gccgcatcac
     3901 cagtcgtcgt cggggtcatc gaacagcgtg atgggttcgc gttcggcagg agcgggcagt
     3961 gtttcggtca gctccagcat ggtgctgcga tagtcggggg gcatgggggt gaccggcggg
     4021 tgttcgggtg gggtgtgctt gtccgggcca cacaaccgca gcgtgtggtg gacccgcgca
     4081 gcacgtcggc gtggcgctcg ccgcaggcgg tgccgtctcc gctgcggttg tccgaccaca
     4141 gtggtacctc tgtgaccagt gaacaagtgt cgtggcggct ggcgtagtcg aggctggagg
     4201 catccagccg ccaggtgttc gctgcatccg atcgcgccag aacggcctgt tgtacgtcct
     4261 gatgggtcgg ggcacggaaa acaccagggg ccagctctta cctgccgcgc caacccacgg
     4321 ggaccgctgt tggagcacaa cgcccaaaga atcgccatcg ccgcggccgc ccaggcgcgt
     4381 gggctcatcc agcgcagcct gacccacgac gtacccatcg acgcccgcct gctcaccgaa
     4441 tcctgcgccg ccctcgccac cttgtcccag taaaatcctc gacgatgtca gctgagcgaa
     4501 gaggcgagac ggcacggtga tgatggagat ttcgggtctc gtcgaagttc cggtcgggtt
     4561 gctcttcatc gtgataccgg ctgtcgcggg ggccgtcgtc acgactcgca tggtcagacg
     4621 gcggcgcacc tggacacatg tctccgggac cgtctcgagt gtgaagacga aacgggctga
     4681 caacgggacg acgaccacca ccgttcgata ccgcttcgtg gatgcgaacg gcgagcaaca
     4741 cagtggctcc gaaaccgccc ttcgcacgcc gcggaaaggc aagaaggtcg gggtcatgta
     4801 caacccggac aatcccgacg acaacgaact ctcctcgacc gcgttcctca tactcgtgct
     4861 gcccctcttg gcggtcatga tcgggttcgg tgtgtacatg ctcctgttat cgatttcagg
     4921 gggtacatat tgcggaactc ccaacctgga cgccccgagc cgggctggca ccaggttggg
     4981 agttctacat cagacgcccg ctatacgaga gcggcgtagc tgcaggcaac gctgcgtgag
     5041 acctcaatgg aggtcttcgc ccagattcca ggattctgcc aggtttcggc tcgcttgttg
     5101 ccgccggcga cgcgtgcccc gtggaccttg gtgtcgtgcc ggtagtcgga gtagcaccgc
     5161 ttgttgccgt tggagaggtt gacgtaaccg cggcaccaaa caccaccgcc gacgccggag
     5221 caggattgca ccgtcacgtc gccgctgttc gaggtaccgc cgccgggaga cgcctgcgcg
     5281 gctgaggcgc caccgagcat catcatgatc accatcgtgg cgacggcggc gcccttggcg
     5341 acaagagttg tcacggacct ggttcgaggt gaagacaaag tcttcctcct atgtagagtt
     5401 cggacttctt gatggactga cgaccgcgtg gccgatgtcc gtcgctcaaa cctccacggg
     5461 tagtcgattc gctaccagga acaggcccgg aacaagagcg gcaactgctc cgcgacacca
     5521 aaccttgaga gaatctggaa tcccctggta gaagcgggat ctcggcgatt ggcggcagct
     5581 gctgaaggtt ttagagttgt atgccgaacc gtcgatgagg acatgaccgg cacgacggcg
     5641 gtgctcgccc tctcagaaag tcacggtcat gaatgcgaat ggattcacca tcggctgcgg
     5701 tcgtctgctg cgtgcccatc ggttgcgggc cggtctggcc caagaggatc tcgcccggat
     5761 ctcgggggtg agtgtgcgcg gcattcggat gatcgagaac gagcagcgac gaccacgggt
     5821 gtccacagta cgcactgtgg cacagtcgct gcggttgtcc gaagccgaca gactcaccct
     5881 tatggaggcg gcccagccgg attctcgtcc cccgcaaaag cccgagaacc acccgcgccg
     5941 tgtggttccc aggcagctgc ccgcatcgcc acgcgggttc gtcgcgcggg aagccgagct
     6001 cggtcgactg gacgcggcac tgaagccgcc cacgagtctg ttgtgtagtg tcaccgggtt
     6061 gggtggtatg ggaaagacct ggctggcggt gcactgggcc accacccggc tcgaccgttt
     6121 tcccgacggc cagttgtatg tgaacctgcg tggcttcgac cctgtcaccg agccgactcc
     6181 cccgtacgtg gggctgcgcg ccatggtgtc cgctttgggg gttccggtgg acagtgtgcc
     6241 cgccgacgtc gatgaactgg cggcgctgtt tcgcagcatc actgcggaca agcgcctgct
     6301 catggtgctg gacaatgccc gtgacgcggc ccaggtggcg ccgatgttgc ctggcggcga
     6361 tggaagcgcc gctgttgtca ccagtcgttc gtcgctgtgc gaactggtga ccgagtacgg
     6421 tgcccgctcg ctgcgtctcg gtggtttgac cgccgacgac tcccggcggt tgcttggcga
     6481 acaatgggat gccgaacgca tcgctcgcga gcccgacgcg gtggaggggc tgatcagccg
     6541 ttgtgccggg cttccgctgg ctttgggaat cgttgcggca cacggagtcc tcaaccccga
     6601 tcacccgttg ggcaccatct atcgaaacct ggtggacgac agcgctaact ccgctgagga
     6661 cggtgatgac atcgtcgacg acattcgcgt ggtcttcgat acctcgctgc gtctggtcgg
     6721 cggcgatgcc tcccggttgt tcgatctgct cggactggcg tccggcagcg gcatcgacgt
     6781 gtacgcggcg gccagcctcg ctggcctgcc ggtgcccgcc gcacgcggtg cgcttcggga
     6841 gttggagaac gcgcacctgg tctcgcggga ctcccccggc cggtaccgca tgcacgatct
     6901 cacgtatcga ttcgcggcgg cgcggagctc ggctgtcatc gacgaaccgg agacggtggc
     6961 ggcgctgcac aggctcatca accactacat tttgaccggg tatcgggccg gtgatgccat
     7021 gcagcaaggc gatgcgcccg tcaccgagaa catcggcctc acaccgcgat gcgtgatcac
     7081 accgatggcc gatcgcgccg cggcctttga ctgggtacgc cgtgagatcg aggattttcc
     7141 gcgataccaa gcgatcgcgc tcgcccatgg ttggtacgaa acggcctggc gagtggccga
     7201 ctgcacccgg ctcttcatcg acgaggacgc catcaatgcg gtggccgagc aggagctgtg
     7261 gcgggtcgcg attgacgccg ccgaacacct gggtcccatg catctggccc gggcacagtc
     7321 gcggttcggc aatcttcgtc tcaaaaccga cccgagtgat tccgaggctg tggatctcat
     7381 gctcaaggcg gcggacttgg ccgcggcgag cggcgaagtg gccgaatgcg cgcgaacaca
     7441 tttcatgctg gcccaccggt ttcaacacaa tggacagaaa gcgcaagcca tcgtccatgc
     7501 gaaactggcc ctcgcattcc tgacggaaac cgaagagccg gtcatgatcg cgcgggtcca
     7561 taacatgatc agctggactc tgggtagcgc gggccggttt cacgacgccc gaagacacgg
     7621 tgaactggct ttggccatcg cccgcgaata cgggctctcc cacaccgaag ccacggtgct
     7681 gggaacactg gggtccaatt cccacaaagc cggtcgacat gcccaagccg aacgactact
     7741 tcggcgggcc tacaccattc actccacaat ggcacagaat ccgtggtccg cgataagcct
     7801 tgaaaacctg ggcgacgtcc tcgccgactc acgacacccc gaccaggcaa gcgaagcgtg
     7861 gcgccgagcg accatccttt tcgaacagac atttcgcatc aaggacgccg agcgagtgcg
     7921 cacgaaactc gattccctgt gatcggttcc gcgccgaagt gttagtggcc gcgatggatg
     7981 atgaaccggt cgtagcggtg gatcccggga gagtcaccga tggcctcgat ggcttcacag
     8041 acgtcgttca cgtcggatac ggacagctgg agtggcggct cttcgggctg atagacagtc
     8101 ctgatcggat agtcttcgct gggggtgcgc gacatttcga tgtggcatcc gaggacgtgt
     8161 gtcacggggt ggttggcgca aaactcgcgc agtttggtga aagtggtgcc gaaatcctgc
     8221 cagtcgtcga cgtacagacg gccggggtac agggtgtcgc cggtcagcag cagaccggtg
     8281 tgcgggtcgt agaagaccgt cgccgagggg tggtgtcccg ggccggggat gacctccaag
     8341 cgtcgtccgc ccaggtccag ggtgcgggag gtgtcgggcc agtcggtgaa tccgtagaac
     8401 tcgaccacct cgtccagatc cgtgccgacg accactgtgt tgggacgatc ggtgaactga
     8461 ccgtccgccg ccttgtgatc gccgtgcccg tgagtgtggg cgaccagcag gccgtagtca
     8521 gggcgcgggt gccgctccag ccaccggtcg atatgcgcgt cgaccgtggc gcgcaacgga
     8581 aagaaccgct ccttcggggt ggcgccggtg tcgatcagga ccgcccggtc gttgccgaac
     8641 agcaggaaca tgaacggtgc ctcgtagtgc acggccttgt tctgccgcat gatcaccgtg
     8701 tgctcgctgt agtggtgcga ctggatctcg tcggccgtgt cgtgtttggc cgacggcgaa
     8761 ccggcgtgcc aggcgacgtc gaggtcaccg gccaccggag cgccacggtc gaaatcgatc
     8821 atggtcgaac cctaatgtcg tgtcagacgc ggccggtctc gtgggcccac atggcgactt
     8881 cgacccggtt acgggcgccg agctttcgca tgagactggc gaggtgggct ttgacggtgc
     8941 tttgggtgat gtagagctcg ctggcgatct cattgttggt gcggccctgg gctaccgaca
     9001 cgaggacttc ctcctcgcgg gcggtgagcc gttctatcgg ctgtggcggt ggtgaggtgt
     9061 gtgtttgggc gaacgccgcg atcagtcgga ccgtcacgct gggtgcgatc agggcgttgc
     9121 cgtcggcggc ggcgtggatg gcctgggtca gcagggcggg tccggcgtcc ttgagcagga
     9181 acccgcgggc gccggctttg agcgcctcgt ggacatactc gtcgaggtcg aaggtggtga
     9241 tgaccacgat cggcaggggg tcggtgacgt cggggccggc gaggcgtcgg gtggcttcga
     9301 tgccgtccat cagtggcatg cggatgtcga acaggccgac gtcggggcgc aactggagcg
     9361 ccaggcggac ggcttcgcgc ccgtcggcgg ccgcgccgac cacttcgatg tcgggctggg
     9421 cgtcgagcag catcgtcagc ccggtgcgca cgatgggctg gtcgtcggcg atgagcaccc
     9481 tgatactcat cacggcgtcc cggttcgggg caggaccgcc tccacccgcc agccccgctc
     9541 ggcggtgggg cccgcgtgga aggtgccgcc gagcagacag gcgcgttccc gcatgcccac
     9601 caggccgtag ccggctgggg cgcggccgcc agccgcggag ccgtcgtcgt cgatggtcag
     9661 ccgtacccgg tcggcgtcgc cggtgacggc gacggtgacc tgggtcgcgt ggcgggcgtg
     9721 ccgtcgcgcg ttggtgatgg attcctgggc caggcggtag atcgcggccc cgaccgccgg
     9781 gctgaggtcg tcgaactcgc cggacagtgt caccgcgacg cggggacgcg tgtggccatc
     9841 ggtggcgagc tgctcgacct cggccacgcc gggctgcggc gcgaacccgg tgtcaggtgg
     9901 agcgcggagc acgccgacga tggcgcgcag ctcggtgagg gtgcgggtcg ccgcgtcctc
     9961 gatgatggcc agggcctcgg cggcacgctc ggggcgggag gcggcgatgg ctcgtcctgc
    10021 ctgggcctgg atggcgatgc ccgagacgtg gtgggcgacg gtgtcgtgga gttcgcgagc
    10081 cagcagttcg cgttcgcggg ccctggcttg gtcgaggtcg cggacgcgga ccttcgcgcg
    10141 atagcggatc gcggcgccga gcgcggcggc gaacaggaag aacgcgtacc ccgcgaccag
    10201 ctccaccacg ttggtcgatt cggcgacgca ggtgatggcc agccagacca ggatgatgcc
    10261 gaggccgccc gcggcttccc gaccggagcc ccaccggaac agggcgtagg cgatgaccag
    10321 ggtggccgcg acactcgagg gcagggtgcc tggc
//
