GCF_000024345.1 - 15 region(s)

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Location: 1,069,441 - 1,103,412 nt. (total: 33,972 nt) Show pHMM detection rules used

NAPAA: (AMP-binding and TIGR02353)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase

KFLA_RS05080: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Lyases: hydratase

KFLA_RS05025: Nitrilase/cyanide hydratase and apolipoprotein

smcogs: SMCOG1294: Nitrilase/cyanide hydratase and apolipoprotein

Ligases: ligase

KFLA_RS05040: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001251.4	0.59	NRPS	epichloenin A	Epichloe festucae
BGC0001833.4	0.59	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002174.2	0.77	NRPS	ε-Poly-L-lysine	Epichloe festucae
BGC0001135.5	0.60	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.60	NRPS	HC-toxin	Alternaria jesenskae
BGC0001261.3	0.60	NRPS	AM-toxin	Alternaria alternata
BGC0001250.4	0.60	NRPS	epichloenin A	Epichloe festucae
BGC0000357.5	0.60	NRPS	cyclo-(D-Phe-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Trp-D-Val-L-Val), cyclo-(D-Phe-L-Phe-D-Val-L-Ile), cyclo-(D-Phe-L-Phe-D-Ile-L-Val), cyclo-(D-Tyr-L-Trp-D-Val-L-Ile), cyclo-(D-Tyr-L-Trp-D-Ile-L-Val), cyclo-(D-Tyr-L-Phe-D-Val-L-Ile), cyclo-(D-Tyr-L-Phe-D-Ile-L-Val)	Penicillium rubens Wisconsin 54-1255
BGC0001251.4	0.59	NRPS	epichloenin A	Epichloe festucae
BGC0001833.4	0.59	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001641.4	0.59	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.59	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 31.36 out of 36.53

Confidence: medium

Additional weak hits (5):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 22.84 out of 30.22

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 18.50 out of 31.73

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 21.02 out of 32.93

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.46 out of 25.75

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.80 out of 28.19

Confidence: weak

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 1069440 - 1103412:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

KFLA_RS05040: X

AMP-binding (20..435): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAELFDASCEELSTIFGGEWNTYGPTEATVVACG
Nearest Stachelhaus code(s):: Lys DAEDIGTVVK (71% 8Å match); Gln or Tyr DAESVGTVVK (65% 8Å match)
Stachelhaus code match:: 90% (moderate)

TFBS definitions

Regulator	Description
DmdR1	Iron(II)-dependent repressor

NC_013729.1 - Region 2 - lanthipeptide-class-iii

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Location: 2,567,867 - 2,590,416 nt. (total: 22,550 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

No tailoring functions detected.

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000496.5	0.67	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000513.3	0.61	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.54	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000518.3	0.51	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000546.3	0.50	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0000528.5	0.45	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001210.5	0.45	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000496.5	0.67	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000513.3	0.61	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.54	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000518.3	0.51	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0000546.3	0.50	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0000528.5	0.45	ribosomal	michiganin A	Clavibacter michiganensis subsp. michiganensis NCPPB 382
BGC0001210.5	0.45	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for KFLA_RS12205

KFLA_RS37060

Leader:

MALLDLQGLEAPTGGGGG

Core:

GGGGSTLTVLGCGSAKPSNLSLLLCH

Core with dehydrations:

GGGGDhaDhbLDhbVLGCysGDhaAKPDhaNLDhaLLLCysH

antiSMASH-DB 4.0 matches for KFLA_RS37060

100.0%

Lanthipeptides: KFLA_RS37060_lanthipeptide

1

GGGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGGSTLTVLGCGSAKPSNLSLLLCH

1

GGGGSTLTVLGCGSAKPSNLSLLLCH

26

92.3%

NC_013595.1:

Lanthipeptides: SROS_RS48825_lanthipeptide

1

GGGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGGSTLTVLGC S KPSNLSLLLCH

1

GGGGSTLTVLGCESYKPSNLSLLLCH

26

88.9%

NZ_JACHWA010000004.1and 1 other:

NZ_PVZV01000008.1 Lanthipeptides: C2865_RS30430_lanthipeptide

Lanthipeptides: FHS43_RS13815_lanthipeptide

2

GGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGSTLTVLGC SAKPSNLSLLLCH

3

GGGSTLTVLGCASAKPSNLSLLLCH

27

with 81 more sequence matches

Only the first 10 are displayed here.

88.5%

NZ_FOQY01000005.1:

Lanthipeptides: BM085_RS08660_lanthipeptide

2

GGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGSTLTVLGC S KPSNLSLLLCH

1

GGGSTLTVLGCESYKPSNLSLLLCH

25

84.6%

NC_013729.1and 1 other:

NZ_CP114065.1 Lanthipeptides: OX958_RS04450_lanthipeptide

Lanthipeptides: KFLA_RS37165_lanthipeptide

3

GGSTLTVLGCGSAKPSNLSLLLCH

26

GGSTLTVLGCGS +PSNLSLLLCH

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

84.6%

Lanthipeptides: DKM19_RS31645_lanthipeptide

1

GGGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGGSTLTVLGC S PSNLSL+LCH

1

GGGGSTLTVLGCASVTPSNLSLILCH

26

84.6%

NZ_CP043661.1and 1 other:

NZ_SOCE01000001.1 Lanthipeptides: EV138_RS20105_lanthipeptide

Lanthipeptides: F1D05_RS17325_lanthipeptide

2

GGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGSTLTVLGC S PSNLSLLLCH

2

GGGSTLTVLGCASHTPSNLSLLLCH

26

84.6%

NZ_SNWQ01000001.1:

Lanthipeptides: EV643_RS00390_lanthipeptide

3

GGSTLTVLGCGSAKPSNLSLLLCH

26

GGSTLTVLGCGS +PSNLSLLLCH

3

GGSTLTVLGCGSHRPSNLSLLLCH

26

81.5%

NZ_JACDUR010000003.1and 1 other:

NZ_JACDUR010000003.1 Lanthipeptides: HNR30_RS17930_lanthipeptide

Lanthipeptides: HNR30_RS17930_lanthipeptide

2

GGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGSTLTVLGC S PSNLSLLLCH

3

GGGSTLTVLGCNSYTPSNLSLLLCH

27

80.8%

BGC0002110.3 (QKV49893.1):

Lanthipeptides: DKM19_RS31650_lanthipeptide

2

GGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGSTLTVLGC S PSNLSL+LCH

1

GGGSTLTVLGCASVTPSNLSLVLCH

25

80.8%

NZ_CP114065.1:

Lanthipeptides: OX958_RS15815_lanthipeptide

1

GGGGSTLTVLGCGSAKPSNLSLLLCH

26

GGGGSTLTVLGC S PSN+SL+LCH

1

GGGGSTLTVLGCESKTPSNISLVLCH

26

80.8%

NZ_SHKR01000011.1and 7 others:

NZ_SLWR01000003.1 Lanthipeptides: EV646_RS09945_lanthipeptideNZ_SNWS01000006.1 Lanthipeptides: EV651_RS15130_lanthipeptideNZ_SODF01000002.1 Lanthipeptides: EV650_RS20180_lanthipeptideNZ_SODP01000004.1 Lanthipeptides: EV653_RS35635_lanthipeptideNZ_SODT01000012.1 Lanthipeptides: EV647_RS27100_lanthipeptideNZ_SODU01000003.1 Lanthipeptides: EV137_RS31660_lanthipeptideNZ_SZPZ01000003.1 Lanthipeptides: FDA38_RS24670_lanthipeptide

Lanthipeptides: EV645_RS10765_lanthipeptide

3

GGSTLTVLGCGSAKPSNLSLLLCH

26

GGSTLTVLGC S PSNLSLLLCH

2

GGSTLTVLGCASQTPSNLSLLLCH

25

80.8%

NZ_SLWN01000005.1:

Lanthipeptides: EV652_RS16625_lanthipeptide

3

GGSTLTVLGCGSAKPSNLSLLLCH

26

GGSTLTVLGC S +PSNLSLLLCH

3

GGSTLTVLGCASQRPSNLSLLLCH

26

MIBiG 4.0 matches for KFLA_RS37060

16.7%

Lanthipeptide: birimositide

1

GGGGSTLT

8

GG TLT

15

NGGWCTLT

22

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.46 out of 20.47

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.15 out of 29.38

Confidence: medium

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 22.88 out of 29.72

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 26.44 out of 28.19

Confidence: strong

Additional weak hits (7):

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.66 out of 29.38

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.48 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.33 out of 28.14

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.72 out of 27.78

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.53 out of 25.88

Confidence: weak

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 20.11 out of 27.55

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 19.36 out of 28.19

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

KFLA_RS37060 - Class III

Cleavage pHMM score: 7.70

RODEO score: 0

Monoisotopic mass: 2333.2 Da

Molecular weight: 2334.7 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2352.7 Da N = 1; 2370.7 Da N = 2; 2388.7 Da N = 3; 2406.8 Da N = 4

TFBS definitions

Regulator	Description
FuR variant 1	Ferric Uptake Regulator
NtrC	Nitrogen regulatory protein C
VqsM	Regulator of quorum-sensing signalling systems
ZuR variant 1	Zinc-responsive repressor

NC_013729.1 - Region 3 - NRPS,T1PKS,T3PKS,transAT-PKS

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Location: 2,645,086 - 2,734,582 nt. (total: 89,497 nt) Show pHMM detection rules used

T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)
transAT-PKS: (cds(PKS_AT and not (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) and (cds(ATd and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS)) or cds(tra_KS and PP-binding)))
NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: Cyclization dehydrogenase oxidoreductase reductase

KFLA_RS12520: Cyclization

MITE: MITE0000095Cyclization (identity: 83.30%)

KFLA_RS12540: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

KFLA_RS12565: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

KFLA_RS12610: oxidoreductase

smcogs: SMCOG1161: oxidoreductase

KFLA_RS12620: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

KFLA_RS12635: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

KFLA_RS12755: alkyl hydroperoxide reductase/ Thiol specific

smcogs: SMCOG1196: alkyl hydroperoxide reductase/ Thiol specific

Transferases: acylase methyltransferase transferase transketolase

KFLA_RS12605: Methylation

MITE: MITE0000155Methylation (identity: 68.10%)
smcogs: SMCOG1150: isoprenylcysteine carboxyl methyltransferase

KFLA_RS12680: acetyl-CoA carboxylase, carboxyl transferase,

smcogs: SMCOG1059: acetyl-CoA carboxylase, carboxyl transferase,

KFLA_RS12700: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

KFLA_RS12745: transketolase

smcogs: SMCOG1204: transketolase

KFLA_RS35525: malonyl CoA-acyl carrier protein transacylase

smcogs: SMCOG1021: malonyl CoA-acyl carrier protein transacylase

Hydrolases: esterase glucosidase

KFLA_RS12730: enterobactin/ferric enterobactin esterase

smcogs: SMCOG1098: enterobactin/ferric enterobactin esterase

KFLA_RS12820: Alpha-glucosidase

smcogs: SMCOG1257: Alpha-glucosidase

Isomerases: mutase

KFLA_RS12835: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: ligase synthase

KFLA_RS12600: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

KFLA_RS12685: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

KFLA_RS12695: 3-oxoacyl-(acyl carrier protein) synthase III

smcogs: SMCOG1084: 3-oxoacyl-(acyl carrier protein) synthase III

KFLA_RS12765: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001135.5	0.92	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.90	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.88	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.88	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.87	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.87	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.86	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.86	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.85	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002286.3	0.85	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000282.3	0.82	PKS	2-methoxy-5-methyl-6-(13-methyltetradecyl)-1,4-benzoquinone, 2-methoxy-5-methyl-6-(13-methyltetradecyl)phenol	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002135.2	0.64	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001166.5	0.63	NRPS	HC-toxin	Alternaria jesenskae
BGC0000307.4	0.63	NRPS	AbT1	Aureobasidium pullulans
BGC0001758.4	0.63	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.62	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001128.4	0.62	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001132.4	0.62	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.62	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.36 out of 20.97

Confidence: medium

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 19.79 out of 25.31

Confidence: medium

Additional weak hits (29):

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.24 out of 29.25

Confidence: weak

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.96 out of 25.88

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.17 out of 32.74

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.50 out of 31.73

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.44 out of 26.06

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 17.69 out of 26.06

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 13.94 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.68 out of 26.06

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.72 out of 30.22

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 16.62 out of 31.73

Confidence: weak

ANR

Description: Anaerobic transcriptional regulator
Species: Pseudomonas putida KT2440
Score: 18.18 out of 26.33

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.57 out of 33.14

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.45 out of 30.98

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.97 out of 24.78

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.04 out of 23.43

Confidence: weak

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.13 out of 39.62

Confidence: weak

HgtR

Description: Hydrogen-dependent regulator
Species: Geobacter sulfurreducens PCA
Score: 17.91 out of 36.54

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.98 out of 29.65

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 21.60 out of 30.98

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.17 out of 29.65

Confidence: weak

NtrC

Description: Nitrogen regulatory protein C
Species: Pseudomonas putida KT2440
Score: 21.74 out of 29.72

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.50 out of 26.06

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.46 out of 35.63

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.38 out of 29.25

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.37 out of 33.14

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.59 out of 32.93

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.00 out of 18.57

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 18.72 out of 35.63

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 3, location 2645085 - 2734582:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 5, location 2668949 - 2734582:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (mal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

KFLA_RS12700: mal

PKS_AT (6..287): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 75.0%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 74.3
inactive: 33.0
Methoxymalonyl-CoA: 31.7
Methylmalonyl-CoA: 26.3
CHC-CoA: 13.4
Benzoyl-CoA: 9.2
2-Methylbutyryl-CoA: 8.1
fatty_acid: 7.9
Isobutyryl-CoA: 5.9
Ethylmalonyl-CoA: 5.3
Propionyl-CoA: 5.1
trans-1,2-CPDA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0

KFLA_RS12765: X

AMP-binding (49..482): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGTS--AFLLYLWAVSGGEVAAWGMTETVTGITF
Nearest Stachelhaus code(s):: Ppa VALFVGAAGK (50% 8Å match); 3clLeu GILWVGAGGK (47% 8Å match)
Stachelhaus code match:: 70% (weak)

KFLA_RS35525: mal - Pro

Search NORINE for peptide: strict or relaxed

PKS_AT (494..777): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 79.2%; inactive: 62.5%; Methylmalonyl-CoA: 58.3%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 125.8
Methylmalonyl-CoA: 61.8
inactive: 55.1
Methoxymalonyl-CoA: 46.1
CHC-CoA: 32.4
Ethylmalonyl-CoA: 30.0
Benzoyl-CoA: 27.2
Propionyl-CoA: 26.3
fatty_acid: 24.7
2-Methylbutyryl-CoA: 23.3
Acetyl-CoA: 21.6
Isobutyryl-CoA: 20.9
trans-1,2-CPDA: 20.1
3-Methylbutyryl-CoA: 17.3

AMP-binding (1580..1972): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LHYTFDVHVQETFMVAGGEYNEYGPTEASVVTTI
Nearest Stachelhaus code(s):: Pro DVQFVAHVVK (59% 8Å match); Trp DVAVVGEVVK (53% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description
CatR	H2O2-responsive repressor
CcpA	Catabolite control protein A

NC_013729.1 - Region 4 - terpene-precursor

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Location: 3,016,888 - 3,037,961 nt. (total: 21,074 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

KFLA_RS14105: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

KFLA_RS14165: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: phosphorylase

KFLA_RS14110: thiamine-phosphate pyrophosphorylase

smcogs: SMCOG1280: thiamine-phosphate pyrophosphorylase

Hydrolases: dehalogenase

KFLA_RS14135: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

Ligases: synthetase

KFLA_RS14145: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.48	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.46	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.46	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.45	terpene	carotenoid	Streptomyces avermitilis
BGC0000647.3	0.45	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001227.3	0.42	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000648.3	0.40	terpene	carotenoid	Myxococcus xanthus
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000638.3	0.39	terpene	carotenoid	Pantoea ananatis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.48	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.46	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001456.3	0.46	terpene	isorenieratene	Streptomyces argillaceus
BGC0000633.3	0.45	terpene	carotenoid	Streptomyces avermitilis
BGC0000647.3	0.45	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000677.5	0.43	terpene	cyclooctatin	Streptomyces melanosporofaciens
BGC0001227.3	0.42	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0000648.3	0.40	terpene	carotenoid	Myxococcus xanthus
BGC0001910.4	0.39	terpene	cyslabdan A, cyslabdan B, cyslabdan C	Streptomyces cyslabdanicus
BGC0000638.3	0.39	terpene	carotenoid	Pantoea ananatis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

KFLA_RS14145: PT_FPPS_like

Domain 1 (45 - 349): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 18.18 out of 20.47

Confidence: medium

Additional weak hits (6):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.15 out of 30.97

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.85 out of 30.97

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 20.57 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 15.75 out of 27.78

Confidence: weak

NuR

Description: Nickel-responsive regulator
Species: Streptomyces coelicolor
Score: 20.72 out of 30.98

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.40 out of 28.14

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
VqsM	Regulator of quorum-sensing signalling systems

NC_013729.1 - Region 5 - NI-siderophore

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Location: 3,381,340 - 3,414,606 nt. (total: 33,267 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

KFLA_RS15755: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

KFLA_RS15775: lysine/ornithine N-monooxygenase

smcogs: SMCOG1080: lysine/ornithine N-monooxygenase

KFLA_RS15840: FAD dependent oxidoreductase

smcogs: SMCOG1103: FAD dependent oxidoreductase

Transferases: kinase methyltransferase

KFLA_RS15790: methyltransferase

smcogs: SMCOG1089: methyltransferase

KFLA_RS15835: glycerol kinase

smcogs: SMCOG1210: glycerol kinase

Hydrolases: hydrolase

KFLA_RS15750: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Lyases: decarboxylase

KFLA_RS15760: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.72	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001478.5	0.62	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.60	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002469.2	0.60	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0001572.3	0.59	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0001531.5	0.58	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002305.2	0.57	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000940.5	0.56	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.56	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000244.4	0.54	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002692.2	0.72	other (other)	speibonoxamine, desoxy-desferrioxamine D1, desferrioxamine D1, desferrioxamine B, didesoxy-desferrioxamine D1, desoxy-desferrioxamine B, didesoxy-desferrioxamine B	Kribbella speibonae
BGC0001478.5	0.62	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0000941.3	0.60	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0002469.2	0.60	other (other)	woodybactin A, woodybactin B, woodybactin C, woodybactin D	Shewanella woodyi ATCC 51908
BGC0001572.3	0.59	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0001531.5	0.58	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002305.2	0.57	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000940.5	0.56	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.56	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0000244.4	0.54	PKS	nonactin, monactin, dinactin, trinactin, tetranactin	Streptomyces griseus subsp. griseus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

Additional weak hits (8):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.66 out of 26.06

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.97 out of 30.22

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.09 out of 30.22

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.27 out of 36.53

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.37 out of 36.53

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.83 out of 28.19

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.34 out of 28.19

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.90 out of 27.04

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator

NC_013729.1 - Region 6 - terpene

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Location: 4,135,554 - 4,159,833 nt. (total: 24,280 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

KFLA_RS19165: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

KFLA_RS19190: dehydrogenase

smcogs: SMCOG1222: dehydrogenase

Transferases: glycosyltransferase methyltransferase

KFLA_RS19170: methyltransferase

smcogs: SMCOG1089: methyltransferase

KFLA_RS39360: glycosyltransferase, MGT family

smcogs: SMCOG1062: glycosyltransferase, MGT family

Hydrolases: hydrolase phosphatase

KFLA_RS19140: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

KFLA_RS19145: inositol monophosphatase

smcogs: SMCOG1207: inositol monophosphatase

KFLA_RS19150: hydrolase

smcogs: SMCOG1179: hydrolase

Ligases: synthetase

KFLA_RS19195: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.66	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.62	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000648.3	0.59	terpene	carotenoid	Myxococcus xanthus
BGC0000650.3	0.58	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000644.3	0.55	terpene	carotenoid	Dietzia sp. CQ4
BGC0000636.3	0.55	terpene	carotenoid	Brevibacterium linens
BGC0000633.3	0.54	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.54	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0001456.3	0.51	terpene	isorenieratene	Streptomyces argillaceus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000664.5	0.66	terpene	isorenieratene	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000649.3	0.62	terpene	carotenoid	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000648.3	0.59	terpene	carotenoid	Myxococcus xanthus
BGC0000650.3	0.58	terpene	carotenoid	Algoriphagus sp. KK10202C
BGC0000647.3	0.57	terpene	carotenoid	Rhodobacter sphaeroides
BGC0000644.3	0.55	terpene	carotenoid	Dietzia sp. CQ4
BGC0000636.3	0.55	terpene	carotenoid	Brevibacterium linens
BGC0000633.3	0.54	terpene	carotenoid	Streptomyces avermitilis
BGC0001227.3	0.54	terpene	isorenieratene	Streptomyces collinus Tu 365
BGC0001456.3	0.51	terpene	isorenieratene	Streptomyces argillaceus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
tetraterpene	C40 carotenoid		C40	unknown	unknown

Genes with terpene domains:

KFLA_RS19175: Lycopene_cycl

Domain 1 (6 - 96): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

KFLA_RS19180: Lycopene_cycl

Domain 1 (4 - 92): Lycopene cyclase

Reactions:: C40_carotenoid → C40_carotenoid

KFLA_RS19185: PT_phytoene_like

Domain 1 (15 - 307): Prenyltransferase; Phytoene synthase like

Subtype: unknown

Reactions:: FPP → squalene; FPP → diapophytoene; FPP → PSPP; GGPP → phytoene; PSPP → hydroxysqualene

KFLA_RS19195: PT_FPPS_like

Domain 1 (38 - 344): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.99 out of 21.55

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 18.35 out of 30.25

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 16.73 out of 28.19

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PSPP	Presqualene diphosphate

TFBS definitions

Regulator	Description

NC_013729.1 - Region 7 - NRPS,T3PKS

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Location: 4,388,290 - 4,468,973 nt. (total: 80,684 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T3PKS: (Chal_sti_synt_C or Chal_sti_synt_N)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase electron transfer oxidoreductase oxygenase

KFLA_RS20350: crotonyl-CoA reductase / alcohol dehydrogenase

smcogs: SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase

KFLA_RS20360: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

KFLA_RS20370: pyridine nucleotide-disulfide oxidoreductase

smcogs: SMCOG1175: pyridine nucleotide-disulfide oxidoreductase

KFLA_RS20385: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

KFLA_RS20390: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

KFLA_RS20480: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

KFLA_RS20490: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

KFLA_RS20495: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

KFLA_RS20585: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

KFLA_RS20625: flavodoxin

smcogs: SMCOG1134: flavodoxin

Transferases: PPTase aminotransferase sulfotransferase

KFLA_RS20520: aminotransferase

smcogs: SMCOG1019: aminotransferase

KFLA_RS20590: Sulfotransferase domain

extras: Sulfotransfer_5: Sulfotransferase domain

KFLA_RS20595: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: back translocase dehalogenase

KFLA_RS20575: haloalkane dehalogenase

smcogs: SMCOG1262: haloalkane dehalogenase

KFLA_RS20610: GTP-binding protein LepA

smcogs: SMCOG1190: GTP-binding protein LepA

Lyases: hydratase lyase

KFLA_RS20415: argininosuccinate lyase/adenylosuccinate lyase

smcogs: SMCOG1063: argininosuccinate lyase/adenylosuccinate lyase

KFLA_RS20525: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

KFLA_RS20530: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

KFLA_RS20540: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Isomerases: isomerase

KFLA_RS20545: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: ligase synthase

KFLA_RS20430: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

KFLA_RS20435: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

KFLA_RS20445: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

KFLA_RS20450: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

KFLA_RS20460: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

KFLA_RS20485: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

KFLA_RS20500: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

KFLA_RS20535: chalcone and stilbene synthase domain protein

smcogs: SMCOG1024: chalcone and stilbene synthase domain protein

KFLA_RS20555: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

KFLA_RS20565: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

KFLA_RS35775: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001249.5	0.84	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001261.3	0.81	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.76	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0002286.3	0.74	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.74	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001128.4	0.73	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002164.2	0.73	NRPS	peramine	Epichloe festucae
BGC0000342.4	0.71	NRPS	enniatin	Fusarium equiseti
BGC0001825.4	0.69	NRPS	xenematide	Xenorhabdus nematophila AN6/1

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001261.3	0.74	NRPS	AM-toxin	Alternaria alternata
BGC0001249.5	0.73	NRPS	dimethylcoprogen	Alternaria alternata
BGC0001132.4	0.72	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.72	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002135.2	0.72	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.72	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001825.4	0.70	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0002164.2	0.69	NRPS	peramine	Epichloe festucae

Similar known clusters from MIBiG 4.0

Similar subclusters

Transcription Factor Binding Sites

Binding sites found:

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 19.37 out of 20.47

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.60 out of 23.79

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.38 out of 19.26

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 18.46 out of 23.05

Confidence: medium

Additional weak hits (19):

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.41 out of 25.75

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.02 out of 18.57

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 19.01 out of 29.25

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 21.22 out of 32.74

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 16.79 out of 28.14

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.16 out of 28.14

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.81 out of 29.38

Confidence: weak

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 15.63 out of 21.55

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.05 out of 28.14

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.29 out of 25.75

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.83 out of 28.14

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 16.42 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 16.41 out of 33.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.43 out of 33.14

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.98 out of 32.93

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 15.00 out of 28.14

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 17.06 out of 33.14

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.19 out of 28.19

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 25.02 out of 36.53

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 9, location 4388289 - 4468973:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - X) + (X) + (Glu) + (X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

KFLA_RS20430: X

AMP-binding (27..412): X

nrpys: dn-dohHep

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAVAFDVSIFDGDLFVGGEVNGYGPTECTVTMIT
Nearest Stachelhaus code(s):: dn-dohHep DVFDFGGVTK (76% 8Å match)
Stachelhaus code match:: 100% (strong)

KFLA_RS20450: X

AMP-binding (501..887): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: NATTFDIAMLELQASCGGDWHTYGPTETTMYTAC
Nearest Stachelhaus code(s):: Ala DILQIGQIYK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

KFLA_RS35775: Pro

Search NORINE for peptide: strict or relaxed

AMP-binding (381..768): Pro

nrpys: Pro

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Cyclic aliphatic chain (polar NH2 group) (Pro, Hpr)
Small clusters prediction:: pro (Pro)
Single AA prediction:: pro (Pro)

Stachelhaus prediction details:

Stachelhaus sequence:: LWQSFDASFQEAFLITAGEDNQYGPTESHVVTAE
Nearest Stachelhaus code(s):: Pro DVQFIAQVVK (79% 8Å match)
Stachelhaus code match:: 90% (moderate)

KFLA_RS20485: Glu

Search NORINE for peptide: strict or relaxed

AMP-binding (21..421): Glu

nrpys: Glu

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: glu,gln (Glu, Gln)
Single AA prediction:: glu (Glu)

Stachelhaus prediction details:

Stachelhaus sequence:: RYMSFDSSVWQWDFLIGGETNTYGPTEASIGMVF
Nearest Stachelhaus code(s):: Glu DVWHVGSIGK (59% 8Å match); ohmeTyr DPWGLGLIDK (53% 8Å match); Asp DSWKLGVVDK (50% 8Å match)
Stachelhaus code match:: 60% (weak)

KFLA_RS20500: X

AMP-binding (461..850): X

nrpys: dn-dohHep

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FAVAFDVSVFEADLFVGGEFNGYGPTECTVTMVV
Nearest Stachelhaus code(s):: dn-dohHep DVFDFGGVTK (85% 8Å match)
Stachelhaus code match:: 100% (strong)

KFLA_RS20565: X - X

AMP-binding (222..624): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: MWSSFAIAAQETVLFATGAFHA--TTETLVTTSC
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

AMP-binding (1287..1685): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Small clusters prediction:: Polar, uncharged (aliphatic with -SH) (Cys)
Single AA prediction:: Polar, uncharged (aliphatic with -SH) (Cys)

Stachelhaus prediction details:

Stachelhaus sequence:: TEASFDLSVYDGNSVLAGDISSGGPAETCIWSVI
Nearest Stachelhaus code(s):: Gly DLYNVASVWK (79% 8Å match)
Stachelhaus code match:: 90% (moderate)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism
VqsM	Regulator of quorum-sensing signalling systems

NC_013729.1 - Region 8 - terpene

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Location: 4,552,958 - 4,577,918 nt. (total: 24,961 nt) Show pHMM detection rules used

terpene: (PT_phytoene_like or phytoene_synt or Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA or TS_Pyr4)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: methyltransferase

KFLA_RS21105: methyltransferase

smcogs: SMCOG1089: methyltransferase

Lyases: cyclase

KFLA_RS21070: Terpene synthase/cyclase metal-binding domain protein

smcogs: SMCOG1052: Terpene synthase/cyclase metal-binding domain protein

Ligases: synthetase

KFLA_RS21060: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001746.3	0.75	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0000657.5	0.58	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0002397.2	0.58	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.54	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.47	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0002309.2	0.46	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002488.3	0.44	other (other)	bacillopaline	Paenibacillus mucilaginosus KNP414
BGC0002568.2	0.42	other (other)	Ethylenediaminesuccinic acid hydroxyarginine	Streptomyces scabiei 87.22
BGC0000640.3	0.41	terpene	carotenoid	Enterobacteriaceae bacterium DC404

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001181.4	0.81	terpene	geosmin	Streptomyces coelicolor A3(2)
BGC0001746.3	0.75	terpene	pristinol	Streptomyces pristinaespiralis ATCC 25486
BGC0000657.5	0.58	terpene	2-methylisoborneol	Pseudanabaena sp. dqh15
BGC0002397.2	0.58	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.54	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002398.2	0.47	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0002309.2	0.46	terpene	cattleyene	Streptomyces cattleya NRRL 8057 = DSM 46488
BGC0002488.3	0.44	other (other)	bacillopaline	Paenibacillus mucilaginosus KNP414
BGC0002568.2	0.42	other (other)	Ethylenediaminesuccinic acid hydroxyarginine	Streptomyces scabiei 87.22
BGC0000640.3	0.41	terpene	carotenoid	Enterobacteriaceae bacterium DC404

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Detailed terpene predictions

Product predictions:

Class	Subclass	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene		germacradienol	C15	C1-C10	OH
sesquiterpene		geosmin	C12	C1-C10	OH

Genes with terpene domains:

KFLA_RS21060: PT_FPPS_like

Domain 1 (80 - 361): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

KFLA_RS21070: T1TS + T1TS

Domain 1 (43 - 399): Type 1 terpene synthase

Subtype: Geosmin synthase, N terminal domain

Reactions:: FPP → germacradienol

Domain 2 (400 - 775): Type 1 terpene synthase

Subtype: Geosmin synthase, C terminal domain

Reactions:: germacradienol → geosmin

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (5):

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 14.53 out of 28.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.35 out of 24.78

Confidence: weak

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 20.77 out of 27.18

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.23 out of 29.65

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 18.29 out of 24.78

Confidence: weak

Terpene

TFBS Finder

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate

TFBS definitions

Regulator	Description

NC_013729.1 - Region 9 - cytokinin

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Location: 5,083,961 - 5,117,556 nt. (total: 33,596 nt) Show pHMM detection rules used

cytokinin: (IPPT and Lysine_decarbox)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: acetyltransferase aminotransferase

KFLA_RS23455: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

KFLA_RS23485: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: peptidase

KFLA_RS23375: gamma-glutamyltranspeptidase

smcogs: SMCOG1274: gamma-glutamyltranspeptidase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002633.2	0.42	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0000895.5	0.42	other (other)	citrulline	Lactobacillus plantarum subsp. plantarum
BGC0002148.2	0.41	other (other)	guanitoxin	Sphaerospermopsis torques-reginae ITEP-024
BGC0001591.4	0.41	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0000843.5	0.41	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064
BGC0000906.3	0.40	other (other)	heme D1	Heliophilum fasciatum
BGC0000488.4	0.40	ribosomal	circularin A	Clostridium beijerinckii
BGC0000896.5	0.39	other (other)	cycloserine	Streptomyces lavendulae subsp. lavendulae
BGC0000552.3	0.39	ribosomal	SmbA, SmbB	Streptococcus mutans
BGC0000813.3	0.39	other (other)	K-252a	Nonomuraea longicatena

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002633.2	0.42	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0000895.5	0.42	other (other)	citrulline	Lactobacillus plantarum subsp. plantarum
BGC0002148.2	0.41	other (other)	guanitoxin	Sphaerospermopsis torques-reginae ITEP-024
BGC0001591.4	0.41	other (other)	fatty acid enol ester, N-tetradecanoyl tyrosine	uncultured bacterium CSLC2
BGC0000843.5	0.41	other (non-nrp beta-lactam)	alanylclavam, 2-hydroxymethylclavam, 2-formyloxymethylclavam, clavam-2-carboxylate	Streptomyces clavuligerus ATCC 27064
BGC0000906.3	0.40	other (other)	heme D1	Heliophilum fasciatum
BGC0000488.4	0.40	ribosomal	circularin A	Clostridium beijerinckii
BGC0000896.5	0.39	other (other)	cycloserine	Streptomyces lavendulae subsp. lavendulae
BGC0000552.3	0.39	ribosomal	SmbA, SmbB	Streptococcus mutans
BGC0000813.3	0.39	other (other)	K-252a	Nonomuraea longicatena

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.11 out of 19.52

Confidence: medium

Additional weak hits (1):

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 21.42 out of 36.53

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production

NC_013729.1 - Region 10 - NI-siderophore

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Location: 5,744,915 - 5,774,672 nt. (total: 29,758 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

KFLA_RS26515: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000941.3	0.64	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001478.5	0.56	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002464.3	0.54	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0000940.5	0.54	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.54	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0001531.5	0.54	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002620.2	0.53	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina
BGC0001572.3	0.53	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002305.2	0.52	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000917.3	0.49	other (other)	molybdenum cofactor	Rhodobacter capsulatus

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000941.3	0.64	other (other)	desferrioxamin B	Streptomyces griseus subsp. griseus NBRC 13350
BGC0001478.5	0.56	other (other)	desferrioxamine E	Streptomyces sp. ID38640
BGC0002464.3	0.54	other (other)	alcaligin	Bordetella pertussis Tohama I
BGC0000940.5	0.54	other (other)	desferrioxamin B, desferrioxamine E	Streptomyces coelicolor A3(2)
BGC0001453.5	0.54	other (other)	desferrioxamine	Streptomyces argillaceus
BGC0001531.5	0.54	other (other)	bisucaberin B	Tenacibaculum mesophilum
BGC0002620.2	0.53	other (other)	fulvivirgamide A2, fulvivirgamide B2, fulvivirgamide B3, fulvivirgamide B4	Fulvivirga marina
BGC0001572.3	0.53	other (other)	desferrioxamine E	Pantoea agglomerans
BGC0002305.2	0.52	other (other)	legonoxamine A, desferrioxamine B, legonoxamine B	Streptomyces sp.
BGC0000917.3	0.49	other (other)	molybdenum cofactor	Rhodobacter capsulatus

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 17.36 out of 20.97

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.58 out of 23.79

Confidence: medium

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 18.39 out of 19.26

Confidence: strong

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.73 out of 23.79

Confidence: strong

NrdR

Description: Represses ribonucleotide reductase encoding genes
Species: Streptomyces coelicolor
Score: 21.83 out of 27.18

Confidence: medium

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 29.86 out of 36.53

Confidence: medium

Additional weak hits (7):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 14.61 out of 21.55

Confidence: weak

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 17.82 out of 30.97

Confidence: weak

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.79 out of 30.22

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 20.42 out of 35.63

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 17.37 out of 28.19

Confidence: weak

DasR

Description: N-acetylglucosamine dependent repressor
Species: Streptomyces coelicolor
Score: 19.50 out of 27.04

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.46 out of 28.19

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
CRP	Cyclic AMP receptor protein
CcpA	Catabolite control protein A
DmdR1	Iron(II)-dependent repressor
IolR	Regulator of the inositol catabolism
NrdR	Represses ribonucleotide reductase encoding genes

NC_013729.1 - Region 11 - arylpolyene

Download region SVG

Location: 6,500,234 - 6,541,316 nt. (total: 41,083 nt) Show pHMM detection rules used

arylpolyene: (APE_KS1 or APE_KS2)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: PPTase methyltransferase

KFLA_RS30065: O-methyltransferase

smcogs: SMCOG1042: O-methyltransferase

KFLA_RS30140: 4'-phosphopantetheinyl transferase

smcogs: SMCOG1012: 4'-phosphopantetheinyl transferase

Hydrolases: hydrolase

KFLA_RS30040: alpha/beta hydrolase domain-containing protein

smcogs: SMCOG1066: alpha/beta hydrolase domain-containing protein

KFLA_RS30165: beta-lactamase

smcogs: SMCOG1053: beta-lactamase

Ligases: ligase synthase

KFLA_RS30050: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

KFLA_RS30085: 1-deoxy-D-xylulose-5-phosphate synthase

smcogs: SMCOG1110: 1-deoxy-D-xylulose-5-phosphate synthase

KFLA_RS30110: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

KFLA_RS30115: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

KFLA_RS30125: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

KFLA_RS30145: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.57	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.57	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.56	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.56	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000196.4	0.47	PKS	AQ-256, AQ-270a, AQ-284a	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002711.2	0.47	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0002591.2	0.45	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.
BGC0000261.5	0.44	PKS	R1128	Streptomyces sp. R1128
BGC0001736.3	0.44	NRPS	pepteridine A, pepteridine B	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001987.2	0.44	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis

Reference	Similarity score	Type	Compound(s)	Organism
BGC0001273.3	0.57	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.57	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.56	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001244.3	0.56	PKS	(-)-Mellein	Parastagonospora nodorum
BGC0000196.4	0.47	PKS	AQ-256, AQ-270a, AQ-284a	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002711.2	0.47	PKS	nostovalerolactone, 9-dehydronostovalerolactone	Nostoc punctiforme PCC 73102
BGC0002591.2	0.45	PKS, terpene	aurachin C, aurachin D, aurachin SS	Streptomyces sp.
BGC0000261.5	0.44	PKS	R1128	Streptomyces sp. R1128
BGC0001736.3	0.44	NRPS	pepteridine A, pepteridine B	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001987.2	0.44	PKS	koreenceine A, koreenceine B, koreenceine C, koreenceine D	Pseudomonas koreensis

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.52 out of 23.79

Confidence: medium

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.26 out of 19.26

Confidence: medium

Additional weak hits (9):

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 18.29 out of 25.88

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 17.89 out of 31.73

Confidence: weak

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 16.40 out of 27.78

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 23.96 out of 31.73

Confidence: weak

MatP

Description: Cell cycle regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 22.13 out of 25.75

Confidence: weak

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 18.21 out of 28.19

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 18.47 out of 33.14

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 17.97 out of 24.78

Confidence: weak

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS substrates

TFBS Finder

NRPS/PKS substrate predictions

KFLA_RS30050: X

AMP-binding (25..422): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LAASFASGQITFYVNVGAGRIVYGLSEVIVVTAQ
Nearest Stachelhaus code(s):: Leu or Ile DAIYLGVVLK (29% 8Å match)
Stachelhaus code match:: 60% (weak)

KFLA_RS30115: X

AMP-binding (45..435): X

nrpys: (unknown)

SVM prediction details:

Predicted physicochemical class:: N/A
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: AHLLHTATILMQYGFNTGD-----------TVTR
Nearest Stachelhaus code(s):
Stachelhaus code match:: 0% (weak)

KFLA_RS30145: X

AMP-binding (54..377): X

nrpys: Gly, Ala, Val, Leu, Ile, Abu, Ival

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: ERYPLGLVGGLGLGTTGGEGNMYGMTEVGVIGTD
Nearest Stachelhaus code(s):: nohBza GLGLVGAIIK (62% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism

NC_013729.1 - Region 12 - NRPS,T1PKS

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Location: 6,756,999 - 6,807,492 nt. (total: 50,494 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase oxygenase

KFLA_RS31455: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

KFLA_RS31515: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

KFLA_RS31530: 3-hydroxyisobutyrate dehydrogenase

smcogs: SMCOG1100: 3-hydroxyisobutyrate dehydrogenase

KFLA_RS31535: cytochrome P450

smcogs: SMCOG1007: cytochrome P450

Transferases: aminotransferase

KFLA_RS31440: aminotransferase class-III

smcogs: SMCOG1013: aminotransferase class-III

Hydrolases: hydrolase protease

KFLA_RS31450: alkaline serine protease, subtilase family

smcogs: SMCOG1075: alkaline serine protease, subtilase family

KFLA_RS31495: isochorismatase

smcogs: SMCOG1027: isochorismatase

Isomerases: mutase

KFLA_RS31435: phosphoglycerate mutase

smcogs: SMCOG1101: phosphoglycerate mutase

Ligases: synthase

KFLA_RS31470: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Translocases: transport

KFLA_RS31485: MMPL domain-containing transport protein

smcogs: SMCOG1035: MMPL domain-containing transport protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.68	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.64	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.63	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.62	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000307.4	0.62	NRPS	AbT1	Aureobasidium pullulans
BGC0001825.4	0.62	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.62	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.62	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001160.5	0.57	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.66	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.65	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001132.4	0.65	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0000307.4	0.65	NRPS	AbT1	Aureobasidium pullulans
BGC0001825.4	0.64	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001758.4	0.64	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.64	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001641.4	0.63	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CRP

Description: Cyclic AMP receptor protein
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.67 out of 19.26

Confidence: medium

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 20.66 out of 23.43

Confidence: medium

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 18.80 out of 23.79

Confidence: medium

Additional weak hits (8):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.13 out of 39.62

Confidence: weak

MexT

Description: Global virulence regulator
Species: Pseudomonas aeruginosa PAO1
Score: 16.40 out of 21.84

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 16.64 out of 29.38

Confidence: weak

NrtR

Description: NAD synthesis repressor
Species: Streptomyces coelicolor
Score: 19.70 out of 35.63

Confidence: weak

BldD

Description: Development and antibiotic global regulator
Species: Streptomyces coelicolor
Score: 19.95 out of 23.43

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.83 out of 29.25

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.52 out of 29.25

Confidence: weak

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 16.94 out of 19.52

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 14, location 6756998 - 6807492:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (X - mal - X)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

KFLA_RS31470: X - mal - X

AMP-binding (43..453): X

nrpys: Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FGSLGALLLYHPWSVSGGERPAWGMAETATAITI
Nearest Stachelhaus code(s):: norCMA DPYWWGATFK (32% 8Å match)
Stachelhaus code match:: 60% (weak)

PKS_AT (1190..1460): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 58.3%; 2-Rhyd-Malonyl-CoA: 54.2%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 114.8
Methoxymalonyl-CoA: 59.6
inactive: 58.5
Methylmalonyl-CoA: 54.8
Propionyl-CoA: 48.6
2-Methylbutyryl-CoA: 32.2
Isobutyryl-CoA: 30.4
Acetyl-CoA: 26.0
Ethylmalonyl-CoA: 24.3
Benzoyl-CoA: 22.4
CHC-CoA: 21.1
trans-1,2-CPDA: 19.2
3-Methylbutyryl-CoA: 12.4
fatty_acid: 0.0

AMP-binding (2545..2925): X

nrpys: Phe, Trp, Ph-Gly, Tyr, bOH-Tyr

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aromatic side chain (Phe, Trp, Ph-Gly, Tyr, bOH-Tyr)
Small clusters prediction:: N/A
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: HQYAFDGSVHETVMLTAGEYNGYGPTEASIGVTE
Nearest Stachelhaus code(s):: Phe DAWVLAGIQK (56% 8Å match)
Stachelhaus code match:: 70% (weak)

TFBS definitions

Regulator	Description
BldD	Development and antibiotic global regulator
CRP	Cyclic AMP receptor protein
IolR	Regulator of the inositol catabolism

NC_013729.1 - Region 13 - lanthipeptide-class-iii

Download region SVG

Location: 6,950,628 - 6,973,195 nt. (total: 22,568 nt) Show pHMM detection rules used

lanthipeptide-class-iii: (micKC and not PKS_KS)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

KFLA_RS32255: acyl-CoA dehydrogenase

smcogs: SMCOG1006: acyl-CoA dehydrogenase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000496.5	0.67	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000513.3	0.61	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.54	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000518.3	0.51	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002111.3	0.48	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139
BGC0000546.3	0.47	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0002698.2	0.44	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000496.5	0.67	ribosomal	AmfS	Streptomyces griseus subsp. griseus NBRC 13350
BGC0000551.5	0.65	ribosomal	SapB	Streptomyces coelicolor A3(2)
BGC0000501.3	0.61	ribosomal	catenulipeptin	Catenulispora acidiphila DSM 44928
BGC0000513.3	0.61	ribosomal	Ery-9, Ery-6, Ery-8, Ery-7, Ery-5, Ery-4, Ery-3	Saccharopolyspora erythraea NRRL 2338
BGC0000519.4	0.60	ribosomal	labyrinthopeptin A2, labyrinthopeptin A1, labyrinthopeptin A3	Actinomadura namibiensis
BGC0000553.3	0.54	ribosomal	SRO15-2212	Streptomyces filamentosus NRRL 15998
BGC0000518.3	0.51	ribosomal	informatipeptin	Streptomyces viridochromogenes DSM 40736
BGC0002111.3	0.48	ribosomal	andalusicin A, andalusicin B	Bacillus thuringiensis serovar andalousiensis NRRL B-23139
BGC0000546.3	0.47	ribosomal	SAL-2242	Streptomyces albidoflavus
BGC0002698.2	0.44	ribosomal	phaeornamide	Pseudophaeobacter arcticus DSM 23566

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for KFLA_RS32285

KFLA_RS37165

Leader:

MALLDLQGLETPGYGHGGHH

Core:

HGGSTLTVLGCGSQRPSNLSLLLCH

Core with dehydrations:

HGGDhaDhbLDhbVLGCysGDhaQRPDhaNLDhaLLLCysH

antiSMASH-DB 4.0 matches for KFLA_RS37165

100.0%

NC_013729.1and 1 other:

NZ_CP114065.1 Lanthipeptides: OX958_RS04450_lanthipeptide

Lanthipeptides: KFLA_RS37165_lanthipeptide

1

HGGSTLTVLGCGSQRPSNLSLLLCH

25

HGGSTLTVLGCGSQRPSNLSLLLCH

1

HGGSTLTVLGCGSQRPSNLSLLLCH

25

92.3%

NZ_SLWN01000005.1:

Lanthipeptides: EV652_RS16625_lanthipeptide

1

HGGSTLTVLGCGSQRPSNLSLLLCH

25

HGGSTLTVLGC SQRPSNLSLLLCH

2

HGGSTLTVLGCASQRPSNLSLLLCH

26

92.3%

NZ_SNWQ01000001.1:

Lanthipeptides: EV643_RS00390_lanthipeptide

1

HGGSTLTVLGCGSQRPSNLSLLLCH

25

HGGSTLTVLGCGS RPSNLSLLLCH

2

HGGSTLTVLGCGSHRPSNLSLLLCH

26

with 68 more sequence matches

Only the first 10 are displayed here.

92.0%

NZ_SHKR01000011.1and 6 others:

NZ_SNWS01000006.1 Lanthipeptides: EV651_RS15130_lanthipeptideNZ_SODF01000002.1 Lanthipeptides: EV650_RS20180_lanthipeptideNZ_SODP01000004.1 Lanthipeptides: EV653_RS35635_lanthipeptideNZ_SODT01000012.1 Lanthipeptides: EV647_RS27100_lanthipeptideNZ_SODU01000003.1 Lanthipeptides: EV137_RS31660_lanthipeptideNZ_SZPZ01000003.1 Lanthipeptides: FDA38_RS24670_lanthipeptide

Lanthipeptides: EV645_RS10765_lanthipeptide

1

HGGSTLTVLGCGSQRPSNLSLLLCH

25

HGGSTLTVLGC SQ PSNLSLLLCH

1

HGGSTLTVLGCASQTPSNLSLLLCH

25

84.6%

Lanthipeptides: KFLA_RS37060_lanthipeptide

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

GGSTLTVLGCGS +PSNLSLLLCH

3

GGSTLTVLGCGSAKPSNLSLLLCH

26

81.5%

NZ_SLWR01000003.1:

Lanthipeptides: EV646_RS09945_lanthipeptide

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

GGSTLTVLGC SQ PSNLSLLLCH

4

GGSTLTVLGCASQTPSNLSLLLCH

27

80.8%

NC_013595.1and 1 other:

NZ_FOQY01000005.1 Lanthipeptides: BM085_RS08660_lanthipeptide

Lanthipeptides: SROS_RS48825_lanthipeptide

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

GGSTLTVLGC S +PSNLSLLLCH

3

GGSTLTVLGCESYKPSNLSLLLCH

26

80.8%

NZ_CP043661.1and 1 other:

NZ_SOCE01000001.1 Lanthipeptides: EV138_RS20105_lanthipeptide

Lanthipeptides: F1D05_RS17325_lanthipeptide

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

GGSTLTVLGC S PSNLSLLLCH

3

GGSTLTVLGCASHTPSNLSLLLCH

26

80.0%

Lanthipeptides: DKM19_RS31650_lanthipeptide

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

GGSTLTVLGC S PSNLSL+LCH

2

GGSTLTVLGCASVTPSNLSLVLCH

25

80.0%

NZ_SLWR01000002.1:

Lanthipeptides: EV646_RS08070_lanthipeptide

3

GSTLTVLGCGSQRPSNLSLLLCH

25

GSTLTVL CGSQRPSNLS++LCH

1

GSTLTVLTCGSQRPSNLSVVLCH

23

77.8%

NZ_JACDUR010000003.1and 1 other:

NZ_JACDUR010000003.1 Lanthipeptides: HNR30_RS17930_lanthipeptide

Lanthipeptides: HNR30_RS17930_lanthipeptide

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

GGSTLTVLGC S PSNLSLLLCH

4

GGSTLTVLGCNSYTPSNLSLLLCH

27

77.8%

NZ_JACHWA010000004.1and 1 other:

NZ_PVZV01000008.1 Lanthipeptides: C2865_RS30430_lanthipeptide

Lanthipeptides: FHS43_RS13815_lanthipeptide

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

GGSTLTVLGC S +PSNLSLLLCH

4

GGSTLTVLGCASAKPSNLSLLLCH

27

76.9%

Lanthipeptides: DKM19_RS31645_lanthipeptide

2

GGSTLTVLGCGSQRPSNLSLLLCH

25

GGSTLTVLGC S PSNLSL+LCH

3

GGSTLTVLGCASVTPSNLSLILCH

26

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

AbrC3

Description: Antibiotic production activator
Species: Streptomyces coelicolor
Score: 18.16 out of 18.16

Confidence: strong

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 17.22 out of 18.46

Confidence: strong

AfsQ1

Description: Two-component system AfsQ1-Q2, activator of antibiotic production
Species: Streptomyces coelicolor
Score: 17.62 out of 19.52

Confidence: medium

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 15.86 out of 18.57

Confidence: medium

Additional weak hits (7):

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 16.32 out of 21.55

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.44 out of 29.65

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.35 out of 26.85

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 17.18 out of 29.25

Confidence: weak

RutR

Description: Regulator of the pyrimidine and purine metabolism
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.90 out of 26.85

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 14.13 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 15.86 out of 33.14

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

KFLA_RS37165 - Class III

Cleavage pHMM score: 4.60

RODEO score: 0

Monoisotopic mass: 2441.3 Da

Molecular weight: 2442.8 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2460.8 Da N = 1; 2478.8 Da N = 2; 2496.9 Da N = 3; 2514.9 Da N = 4

TFBS definitions

Regulator	Description
AbrC3	Antibiotic production activator
AfsQ1	Two-component system AfsQ1-Q2, activator of antibiotic production
RpoN	Regulator of nitrogen assimilation and virulence
ToxT	Regulator of MSHA biosynthesis

NC_013729.1 - Region 14 - lanthipeptide-class-ii

Download region SVG

Location: 6,982,599 - 7,005,490 nt. (total: 22,892 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

TTA codons

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase oxidoreductase

KFLA_RS32410: aldehyde dehydrogenase

smcogs: SMCOG1017: aldehyde dehydrogenase

KFLA_RS32415: oxidoreductase

smcogs: SMCOG1083: oxidoreductase

Transferases: acetyltransferase

KFLA_RS32385: GCN5-related N-acetyltransferase

smcogs: SMCOG1111: GCN5-related N-acetyltransferase

Hydrolases: deacetylase

KFLA_RS32390: polysaccharide deacetylase

smcogs: SMCOG1235: polysaccharide deacetylase

Lyases: cyclase

KFLA_RS32420: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Translocases: permease

KFLA_RS32395: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002704.2	0.47	ribosomal	archalan β	Halorussus salinus
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000500.5	0.41	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0001407.5	0.41	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0000527.5	0.41	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001229.4	0.40	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0000516.4	0.40	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0001210.5	0.40	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002362.3	0.39	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002704.2	0.47	ribosomal	archalan β	Halorussus salinus
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0000500.5	0.41	ribosomal	carnolysin A1, carnolysin A2	Carnobacterium maltaromaticum
BGC0001407.5	0.41	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0000527.5	0.41	ribosomal	mersacidin	Bacillus sp. HIL-Y85/54728
BGC0001229.4	0.40	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0000516.4	0.40	ribosomal	geobacillin II	Geobacillus thermodenitrificans
BGC0001210.5	0.40	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0002362.3	0.39	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for KFLA_RS32420

KFLA_RS32425

Leader:

MTAQHPHDENLAQPATDLEGTDPLGPVTLPKRSKIGRRALALVGT

Core:

TSMAALAVQTLADGCSTFTTVGHEL

Core with dehydrations:

DhbDhaMAALAVQDhbLADGCysDhaDhbFDhbDhbVGHEL

antiSMASH-DB 4.0 matches for KFLA_RS32425

100.0%

Lanthipeptides: KFLA_RS32425_lanthipeptide

1

TSMAALAVQTLADGCSTFTTVGHEL

25

TSMAALAVQTLADGCSTFTTVGHEL

1

TSMAALAVQTLADGCSTFTTVGHEL

25

44.0%

NZ_SNWQ01000001.1:

Lanthipeptides: EV643_RS00525_lanthipeptide

2

SMAALAVQTLADGCSTFTTV

21

S+AA AV D CST TT+

2

SVAAFAVHAAIDYCSTDTTI

21

36.0%

NZ_CP043661.1:

Lanthipeptides: F1D05_RS17455_lanthipeptide

2

SMAALAVQTLADGCSTFTTV

21

S A+ V + DGC TFT++

2

SALAVGVIEIVDGCGTFTSI

21

with 1 more sequence matches

24.0%

NZ_SOCE01000001.1:

Lanthipeptides: EV138_RS19955_lanthipeptide

2

SMAALAVQTLADGCSTFTTV

21

S A+ + + + C TFT++

2

SALAVGIVEVTENCGTFTSI

21

allorf_6984235_6984345

Leader:

MGRWAGGGHRNTLHAG

Core:

TVDRRYPDPRALSEPAACRR

Core with dehydrations:

DhbVDRRYPDPRALDhaEPAACysRR

antiSMASH-DB 4.0 matches for allorf_6984235_6984345

100.0%

Lanthipeptides: allorf_6984235_6984345_lanthipeptide

1

TVDRRYPDPRALSEPAACRR

20

TVDRRYPDPRALSEPAACRR

1

TVDRRYPDPRALSEPAACRR

20

40.0%

NZ_CP096021.1:

Lanthipeptides: allorf_398029_398259_lanthipeptide

9

PRALSEPAACRR

20

PR+LS P+AC R

4

PRSLSVPSACDR

15

23.3%

NZ_JANYMP010000042.1:

Lanthipeptides: allorf_49545_49673_lanthipeptide

7

PDPRALSEPAA

17

PDPR+ S P A

9

PDPRSRSTPTA

19

allorf_6992193_6992393

Leader:

MARDRDGSRWSWSSWPASRCRVELLERRERCLRRQRPGKSRRPPSFGA

Core:

GWGCGTWGWIPHVRSADR

Core with dehydrations:

GWGCysGDhbWGWIPHVRDhaADR

antiSMASH-DB 4.0 matches for allorf_6992193_6992393

100.0%

Lanthipeptides: allorf_6992193_6992393_lanthipeptide

1

GWGCGTWGWIPHVRSADR

18

GWGCGTWGWIPHVRSADR

1

GWGCGTWGWIPHVRSADR

18

allorf_6992297_6992518

Leader:

MSGKRLFCCCPRDGDWGLTAPRQQDLSRAASGGRLSGSGIQVWRETVTVPAG

Core:

RGRRGRPPAVGSNCWNAENDA

Core with dehydrations:

RGRRGRPPAVGDhaNCysWNAENDA

antiSMASH-DB 4.0 matches for allorf_6992297_6992518

100.0%

Lanthipeptides: allorf_6992297_6992518_lanthipeptide

1

RGRRGRPPAVGSNCWNAENDA

21

RGRRGRPPAVGSNCWNAENDA

1

RGRRGRPPAVGSNCWNAENDA

21

33.3%

NZ_CP031746.1:

Lanthipeptides: allorf_1814207_1814410_lanthipeptide

2

GRRGRPPAV

10

GR GRPPA+

4

GRPGRPPAL

12

25.9%

NZ_CP069396.1:

Lanthipeptides: allorf_00877023_00877133_lanthipeptide

6

RPPAVGSNCW

15

RPP GS CW

15

RPPTAGSPCW

24

with 3 more sequence matches

23.3%

NZ_BBXD01000030.1:

Lanthipeptides: allorf_42087_42218_lanthipeptide

2

GRRGRPPAVGSNCWNAEN

19

G PAV SNC + +

1

GHFSFLPAVSSNCCDVYH

18

17.2%

NZ_CP071595.1:

Lanthipeptides: J3S04_RS01545_lanthipeptide

9

AVGSNCWNAEN

19

++GS CW+ N

4

SIGSTCWSCNN

14

15.6%

NZ_CP061007.1:

Lanthipeptides: IAG28_RS29035_lanthipeptide

7

PPAVGSNCW

15

P VG+ CW

12

PITVGTTCW

20

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

Binding sites found:

PsrA

Description: Oleic acid responsive regulator
Species: Pseudomonas sp.
Score: 23.38 out of 25.88

Confidence: strong

Additional weak hits (7):

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.35 out of 32.74

Confidence: weak

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 14.18 out of 18.57

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 18.23 out of 29.38

Confidence: weak

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 19.05 out of 24.78

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 20.58 out of 32.93

Confidence: weak

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 13.69 out of 26.06

Confidence: weak

HypR

Description: L-hydroxyproline utilization repressor
Species: Streptomyces coelicolor
Score: 18.94 out of 22.40

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

KFLA_RS32425 - Class II

Cleavage pHMM score: -7.20

RODEO score: 0

Monoisotopic mass: 2397.2 Da

Molecular weight: 2398.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2416.7 Da N = 1; 2434.7 Da N = 2; 2452.7 Da N = 3; 2470.8 Da N = 4; 2488.8 Da N = 5; 2506.8 Da N = 6

allorf_6984235_6984345 - Class II

Cleavage pHMM score: -4.10

RODEO score: 0

Monoisotopic mass: 2292.2 Da

Molecular weight: 2293.6 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2311.6 Da N = 1

allorf_6992193_6992393 - Class II

Cleavage pHMM score: -5.90

RODEO score: 0

Monoisotopic mass: 2004.0 Da

Molecular weight: 2005.2 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2023.2 Da N = 1

allorf_6992297_6992518 - Class II

Cleavage pHMM score: -1.70

RODEO score: 0

Monoisotopic mass: 2264.1 Da

Molecular weight: 2265.4 Da

Number of bridges: 1

TFBS definitions

Regulator	Description
PsrA	Oleic acid responsive regulator

NC_013729.1 - Region 15 - hydrogen-cyanide