LOCUS       NC_010629              10228 bp    DNA     circular CON 15-MAR-2024
DEFINITION  Nostoc punctiforme PCC 73102 plasmid pNPUN05, complete sequence.
ACCESSION   NC_010629
VERSION     NC_010629.1
KEYWORDS    RefSeq.
SOURCE      Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)
  ORGANISM  Nostoc punctiforme PCC 73102
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae;
            Nostoc.
REFERENCE   1  (bases 1 to 26419)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L.,
            Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E.,
            Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M.
            and Atlas,R.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of plasmid5 of Nostoc punctiforme ATCC 29133
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 26419)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L.,
            Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E.,
            Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M.
            and Atlas,R.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2008) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000020025.1-RS_2024_03_15
            Annotation Date                   :: 03/15/2024 03:30:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,702
            CDSs (total)                      :: 7,602
            Genes (coding)                    :: 7,314
            CDSs (with protein)               :: 7,314
            Genes (RNA)                       :: 100
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 133 of 288
            Pseudo Genes (incomplete)         :: 166 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 85 of 288
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:28:06
            NOTE :: This is a single region extracted from a cross-origin
            section of a larger, circular record.
            Orig. start                       :: 18866
            Orig. end                         :: 2675
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP001042.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 2351359
            Source DNA available from John C. Meeks (jcMeeks@ucdavis.edu)
            Bacteria available from ATCC: ATCC 29133
            Contacts: John C. Meeks (jcMeeks@ucdavis.edu)
            David Bruce (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LLNL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     proto_core      5001..5228
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    44..57
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.79"
                     /tool="antismash"
     misc_feature    complement(313..328)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 24.04"
                     /tool="antismash"
     misc_feature    complement(397..407)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
     misc_feature    complement(994..1012)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 23.46"
                     /tool="antismash"
     misc_feature    complement(995..1011)
                     /note="TFBS match to NuR, Nickel-responsive regulator,
                     confidence: weak, score: 24.43"
                     /tool="antismash"
     gene            1706..1876
                     /locus_tag="NPUN_RS42660"
     CDS             1706..1876
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NPUN_RS42660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_193372294.1"
                     /transl_table=11
                     /translation="MMQCYALFMFLSASPEYFRPVDPSLYGQWSDERISIESALTGAHV
                     KLGFWGVRLSS"
     gene            complement(2149..3015)
                     /locus_tag="NPUN_RS37235"
                     /old_locus_tag="Npun_ER021"
     CDS             complement(2149..3015)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407239.1"
                     /locus_tag="NPUN_RS37235"
                     /note="PEP-CTERM proteins occur, often in large numbers, in
                     the proteomes of bacteria that also encode an exosortase, a
                     predicted intramembrane cysteine proteinase. The presence
                     of a PEP-CTERM domain at a protein's C-terminus predicts
                     cleavage within the sorting domain, followed by covalent
                     anchoring to some some component of the (usually
                     Gram-negative) cell surface. Many PEP-CTERM proteins
                     exhibit an unusual sequence composition that includes large
                     numbers of potential glycosylation sites. Expression of one
                     such protein has been shown restore the ability of a
                     bacterium to form floc, a type of biofilm; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /old_locus_tag="Npun_ER021"
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="WP_234711221.1"
                     /transl_table=11
                     /translation="MASQGAYASDLKGTAFSISLECMNDTTGLLMGQNSTDANGWQYAF
                     DSNKDGMNGNYWVGAAPGSVNPYDISGMAIKETATSIIVAINGNMKLTGEAEQGAAGGQ
                     IGYGDLFFNMAGKTFDNAMSSGDLFGIHFASANASGVQQLGVYSGVQAKTVTNIKEGYT
                     VATYGGLAGGGNSYESQVKQGGGIVGYGDLTSSYFTNNGKDNTFNLNVIDSGKYLSGIS
                     FLAQGNVTQQLLTTGYDASKFNGTQTIAFEFKKSGTVQSTPEPASLAGLGIVGLALAGS
                     KRRKKSS"
     misc_feature    complement(3641..3652)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 17.85"
                     /tool="antismash"
     misc_feature    complement(3857..3875)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 24.29"
                     /tool="antismash"
     misc_feature    3944..3959
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 21.78"
                     /tool="antismash"
     gene            3976..4578
                     /locus_tag="NPUN_RS37240"
                     /old_locus_tag="Npun_EF022"
     CDS             3976..4578
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1077: phage integrase
                     family protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012413382.1"
                     /locus_tag="NPUN_RS37240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_EF022"
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="WP_012412842.1"
                     /transl_table=11
                     /translation="MRPPPPTRLPNKHYRSREYLTPSEVRSLLDAALERKARYAHRDYT
                     LMLLMFRHGLRVGEAVGAKCGLRWDALMWAERQIFITREKGSDSGVHPLRDDELVLLKE
                     LREMLPDSKYIFVSERGEVMSTDAVRKLLGRLAAQAGLDIKVHCHMMRHACGYYLVNQG
                     YNTREIQDFLGHRDIKHTEKYTKLNARRFLNFDWGDL"
     misc_feature    4016..4031
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 20.56"
                     /tool="antismash"
     misc_feature    4761..4776
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: medium, score: 24.6"
                     /tool="antismash"
     gene            5001..5228
                     /locus_tag="NPUN_RS37245"
                     /old_locus_tag="Npun_EF023"
     CDS             5001..5228
                     /GO_component="GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /GO_function="GO:0005488 - binding [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Bacteriocin_IIc"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR03893.1"
                     /locus_tag="NPUN_RS37245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_EF023"
                     /product="lichenicidin A2 family type 2 lantibiotic"
                     /protein_id="WP_012412843.1"
                     /sec_met_domain="Bacteriocin_IIc (E-value: 6.6e-09,
                     bitscore: 27.1, seeds: 35, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKILISDLHSVGEKEVLLSELSDEEMATIQGGSNAPAEVLAVCA
                     VGGAVLGGPAGFLGGAFVGAILATIVASQE"
     gene            complement(5400..6416)
                     /locus_tag="NPUN_RS37250"
                     /old_locus_tag="Npun_ER024"
     CDS             complement(5400..6416)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605407.1"
                     /locus_tag="NPUN_RS37250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_ER024"
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="WP_012412844.1"
                     /transl_table=11
                     /translation="MSDLTVNDGSYQQLLDRIGECLALGQQRSFEQVNSVLVETYWQIG
                     RYIVEFEQAGKERAEYGSKLLQVLSRDLKAAYGKGFSRSNLQYMRLFYLNYENCQTLSG
                     KLSWSHYTELLAISDDLARSFYEQQCIQDRWSVRELKRQKDSALFERVALSKDRAEILA
                     LAKDGQRIESARDVVKDPYVFEFLDLPDRNYRESELENRLIAELEKFLLELGKGFAFIG
                     QQYRITLNNTHFFVDLVFYHRILKCFVLIDLKTRAVSHQDIGQMNMYLNYFRAEENMED
                     DNEPIGIVLARDKDEILVEYAKGGISNQLFVSRYQLYLPDPEVLKQELKRLLSVNDG"
     gene            complement(6519..6794)
                     /locus_tag="NPUN_RS37255"
                     /old_locus_tag="Npun_ER025"
     CDS             complement(6519..6794)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015214264.1"
                     /locus_tag="NPUN_RS37255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_ER025"
                     /product="hypothetical protein"
                     /protein_id="WP_012412845.1"
                     /transl_table=11
                     /translation="MAEVKKSQKLSLRPGVQKTPVKDEAAAWVESRNAAETPSTTGTEE
                     VNNGKSKRVTFEVTEAQHQEIKIRATKKGMTIKEYMIALVEEELKS"
     gene            complement(6781..7431)
                     /gene="parA"
                     /locus_tag="NPUN_RS37260"
                     /old_locus_tag="Npun_ER026"
     CDS             complement(6781..7431)
                     /codon_start=1
                     /gene="parA"
                     /gene_functions="other (smcogs) SMCOG1157: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015214263.1"
                     /locus_tag="NPUN_RS37260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_ER026"
                     /product="ParA family partition ATPase"
                     /protein_id="WP_012412846.1"
                     /transl_table=11
                     /translation="MKIAVLNQKGGSGKTTVSIHLAHAFSMIGYRVLLVDTDPQGSTRD
                     WAAARNGEAPFSVIGLDRPILHKELPKLAQGYDFVFIDGAPRVSDLTRSAIMAVDFVLV
                     PVQPSPLDVWAVHEVVELVQEATIYKPDLTAAFLINRKIANTALGREVTEVLGEYPFPV
                     LKAQISQRVAFAECLNIGSTVLEAAPKSAASDEVRAVVEEILEAREETNHGRS"
     misc_feature    complement(7445..7459)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.5"
                     /tool="antismash"
     region          1..10228
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10228
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10228
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5228](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     gene            8060..8470
                     /locus_tag="NPUN_RS44005"
     CDS             8060..8470
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NPUN_RS44005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_052304757.1"
                     /transl_table=11
                     /translation="MKNFVPTSIALAGLVMLAASAAPIHAQCASPKYVSGVWKANDGGT
                     YRLHAIGSTIWWVGMSGDDGRSWTNVFKGTRNGNIIDGDWADVRGQPWGHGTLKLRISG
                     TTFMEKIDGTGSGFGGIRWGRGGCNDTQGNPG"
     gene            complement(8802..9119)
                     /locus_tag="NPUN_RS37140"
                     /old_locus_tag="Npun_ER001"
     CDS             complement(8802..9119)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406963.1"
                     /locus_tag="NPUN_RS37140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_ER001"
                     /product="hypothetical protein"
                     /protein_id="WP_041566795.1"
                     /transl_table=11
                     /translation="MATREIHLELLLGKQVLDSTGKSAGRIEEVRAEQRGEEWVIQEYL
                     IGTIAVLERLSAWTIGLRILHLFGAHKIHSGYTISWEQLDLTDPEKPRLLCTLHDLKAL
                     K"
     gene            complement(9106..9234)
                     /locus_tag="NPUN_RS44405"
     CDS             complement(9106..9234)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NPUN_RS44405"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_272913993.1"
                     /transl_table=11
                     /translation="MRDVGIDVEVEIEADATPALASEKWLREHIIGRIPGAEHGNS"
     gene            complement(9410..9763)
                     /locus_tag="NPUN_RS42655"
                     /old_locus_tag="Npun_ER002"
     CDS             complement(9410..9763)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015163241.1"
                     /locus_tag="NPUN_RS42655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_ER002"
                     /product="hypothetical protein"
                     /protein_id="WP_012412823.1"
                     /transl_table=11
                     /translation="MNIIRDVLDNQLVDSDQHKLGKVDGIVIELRDGEPPRLSYIETGM
                     TTLARRIHPRLGRWVAAIQSSWGAKRGKVTEAVTRGKVACKYYQTSVSAFIMIGDSDHS
                     PLLKSSDKKWSRN"
ORIGIN
        1 ttgcttacga caaggaattt atcagaacga aagtagcccc agcatttcta aataaaatgc
       61 tgcaagcact taatgacctg atagaagaag gcattgatta cgaatgtaca actgatgctt
      121 tctacaacct tcagaaagaa aataatcacc tatttgactt catagaagcc gcaaacttat
      181 gttatgtccc tggtaaggaa ctgactgcaa aagagctatg ggtaagactg gaacattact
      241 acactcaaac tggcacatta actatagatt ccgataacaa ccgcagaacg tggagcgaac
      301 aggttagacc cagtgacaaa aacgtaaagg gcattaacca agtcatcgcc agaatcgctc
      361 aactcttccc caaggctgtc aagggaacta ggtactgtga cattgctaaa agaaacattc
      421 cagtactcaa aggcattggc attttggaag ttacccgcac cacattagac gaaacccgca
      481 ccacttccgc accactttcc gcaccagaaa caacgcatta tcaggacttc cgcaccaccc
      541 gcaccacttt ttcagattcc caggaaaaaa acattgaaga gtcagaaatg aaaattcaat
      601 cgcttcctat cccaatggaa aaagaagaat atgccacctc aactggtgcg ggtgatgcgg
      661 aacccttgct agatagggaa aactgctgcg gggagtcctg cgtcagtggt gcggatcaac
      721 tagaaagtgg tgcgggtatg ccaactgatg atcaggcagc gatcgccgcg ccaattgcat
      781 ccgatactgt tttggtaaaa gctgaggacg agaacttata ctcactgttg cagattgaaa
      841 tcgacggcgg gaacattgca gggtttgttg gttgccaagt tgaggtgcga tcgcttagtg
      901 gtgcggtcaa gttcgcaggt gagatgatta actacgactc taagaatgga attgtgactg
      961 tggccacctc gcaagggaac cgagatgcat ctctttgcga gacgtttgta attgactaag
     1021 gaatgcaact ggccatcgct actgtcgtga ctgcgcttaa tcgaaagcac ttctaaatta
     1081 ggttgctcca tgtcctgtat gactaccgaa ttacaggaga ttgcgagtaa gccatcgtgt
     1141 tgattgatac caattttcaa tgactgtcaa aatgcgccgt aaactacccc tagtatccct
     1201 ctactgtacc caatgacagt cgtttgacag tcattgccca aaaacccgat gactgtcaaa
     1261 cgactgtcgt tataccccct aaaggtgctt ttgggcgtat tttcgcacca gccagtaagg
     1321 gcgatttctc ccccaaaagt ggatttcagg gttttgtata agtgatacca tactcactat
     1381 ggtttcatgc cctgcgggtg gctgcgccat ctctcgctcc gctcgtgaca tgaaaccgcc
     1441 gttcgcctaa aggggttttt tcgtactaca ctcgcactgc atttgtagca taatttttta
     1501 tgtcgccact aacaattctc aggattgaga aactaaaaac attcggcaac gttgcgggaa
     1561 gtgatgacca tgttaccaga aacagagaaa cacccaacgc agatccaacg cttatagaat
     1621 gtccggttaa ttgggggaga agacgaacgc gcactcgaag agatagttaa agaaaaaatc
     1681 tctacgctac tgcatcgccc tcggcatgat gcagtgttat gcactcttca tgtttctctc
     1741 ggcatcacct gaatatttcc gtcccgttga tccatctctt tatgggcagt ggtctgatga
     1801 gaggatcagt attgagtcag ccctgactgg cgctcacgtc aaattagggt tttggggcgt
     1861 taggctatct tcttgacaaa agctaacttt tccccaggca gtcccccttt tggccaactg
     1921 ttcgctcaac tttcttatag cgaggaaacg cactacatta ttctatataa tggggtttaa
     1981 aaaaagaaat gcccttactt tttagtaaga gcattgaaat ttagttattt aaatgtttaa
     2041 tgggtaaatc gatacagctt gaaatacttt tcgcttaagc tttatccaaa attctattac
     2101 tcactgaaga taaatcaagc tctattgcga atagtctcca ccaaattgct acgatgattt
     2161 cttgcgtcgc ttgctaccag ctaaggccag accgacaatt cctaaaccag cgagactagc
     2221 aggctcagga gtcgattgga cagtaccaga tttcttgaat tcaaatgcaa tagtttgtgt
     2281 gccattaaat ttgctggcat cataaccagt ggttaacaac tgctgcgtta cattaccctg
     2341 tgctaggaac gagattccac tcagatattt tccagagtcg atgacattaa gattaaatgt
     2401 attgtccttg ccattattag taaaataact actggtcaaa tcaccataac cgacaatacc
     2461 accaccttgt tttacctgac tttcgtaaga attccctcct cctgctaaac caccatatgt
     2521 agcaacagta tagccctctt tgatattagt aactgtcttt gcctgaacac cactataaac
     2581 acctagttgt tgtactcctg aagcattcgc tgaagcgaaa tgaataccaa acaaatcccc
     2641 actgctcata gcattgtcaa aggtcttacc ggccatattg aagaataaat caccatatcc
     2701 aatctgaccg ccggcagcac cctgttcagc ctctcctgtt aacttcatat tgccattgat
     2761 tgccacaatg atgctggtag cagtttcttt gatagccata ccagagatat cgtagggatt
     2821 tactgaccct ggcgcagcac caacccagta atttccgttc atcccatctt tgtttgaatc
     2881 aaatgcatac tgccagccat tagcatcggt ggagttttga cccatgagaa gaccagttgt
     2941 gtcattcata cactccaaag atatgctaaa tgctgttccc tttaagtcgg aggcatacgc
     3001 tccttgacta gccacaaaga agattccaaa agtggtaatg ccaatcttta gtaaattgaa
     3061 taagttttga tttttcatat tttataactt gattgattta gaaaatcaca gttttctgta
     3121 cttctctatt tgtaattgga attacaaaaa cagaaaagcg tgattattct gaacatataa
     3181 tttggataaa ttaacgataa ttttataaaa ctcaaataaa aaatggcata tttagtgaaa
     3241 attgcttttc tctaacccaa ttgtattaaa ggtgaaatag gagccaagcc aagtaggtct
     3301 agtagcctac gattttctgc acccatttaa ttagatagtt ttaaaaaact tttgggtttg
     3361 tataagctta acaaaccata tcccgccagc atctccgtac agggcatcta aacccgctat
     3421 cgctagaagt tttttgtcta tattaatcta ctttcagggg tcaaatgagc atccgtgcag
     3481 aaacttacgt taaagctgaa aaccttactg tataggcatt ctgcaaatcg atatttttgg
     3541 caattcttga tgtaccaatt aacctatagg ttaattggta cagctttttt gtatggcact
     3601 acaaggattt gaagttccta aatttcaaag gctttaactg gtaaacgttt ccaggatgtt
     3661 gaaaacaggt catcaagtcc ttagcgtaag tttctgaacg gatgctcatt tcacgccgtt
     3721 ctctagaaat tgctttgatg aagggtgcga tcgtctactt tccgccagaa ctgagggcat
     3781 cgagaagcga tcgccctcat ccacgttgac caatatcatt aaatgtcgcc acagcgatgg
     3841 taatgtacta aaaacaattg ataactattt gcaataactt cttgttggct tgtctgtcaa
     3901 cggagtaatg gggcttgtgg cgtagtagac tgtccataat acaacgccta taccggacac
     3961 taacccgaaa gctaaatgag gccaccgccc ccaactcgtc taccaaacaa gcattacagg
     4021 tctagagaat accttacacc aagtgaagtg cgatcgctgc tcgatgccgc actagaacgc
     4081 aaagctcgtt atgctcaccg tgattataca ctgatgttac tcatgtttcg ccacggtctt
     4141 agggtagggg aagctgtagg ggctaagtgc ggtttaaggt gggatgcact gatgtgggcc
     4201 gagcggcaga ttttcatcac cagggaaaag ggcagtgatt caggggtgca tcccttgaga
     4261 gatgatgaat tagttctcct caaagaactc agagagatgt tgcccgatag caaatatatt
     4321 ttcgtttccg agcgtggtga ggtgatgtcc actgatgcag tgcgaaagct gcttgggcgg
     4381 ctggcggcac aggctgggct tgatatcaag gtacactgcc acatgatgcg ccatgcttgc
     4441 ggttactatc tggtgaatca gggttataac actagagaga ttcaggactt tttaggacac
     4501 cgcgatatca aacacacgga gaaatacacc aagctgaatg ctcggcgctt cctgaatttt
     4561 gattggggag atttgtgaaa tgaagttgcc aattatttaa attatgttcg tacagggttt
     4621 tcagaccagg gtaaattatc cgcgaacaaa aacttaggta tgataaattc ccccagttat
     4681 ttttcaaact gaataaaacg gagttaaacg cctcaagcct aagaaaattt acgcggcgtt
     4741 ttgtttatta ggtgttgatt ttgacggctt tttgacacag acgggtgtaa cgggctaaac
     4801 gcgtttgcct tagaaaaatt cccaggaatt gaagattttt ttaaatactg taaatttatc
     4861 ggtattgtaa gaattaaatt ttttaattct ctttataaag aatattacta aaaataatta
     4921 cgttattttt tctcacttct tatcagtttt catctacaat cagtttgtta atatttattg
     4981 gattaaaagg acttcagaaa atggctaaaa tcctaatttc tgatctgcat tctgttggag
     5041 aaaaagaagt attgctgagt gagctttcgg atgaagaaat ggctaccatt caaggtggta
     5101 gtaatgctcc tgctgaagtc ttagcagttt gcgctgttgg aggagcggta cttggtgggc
     5161 cggcaggttt tctaggtggt gcttttgttg gagctattct agcaacaata gtcgctagtc
     5221 aggaataggt tattcaagta tcaatttaat ttttaattaa gcctctaata catttatgtg
     5281 ttaaaggctt aatttttaga aaaacgattt ttgtaattaa ttttatcttt ttcgcttgat
     5341 tcaatgaaat gtcgtctaat gatagtatga cgacatcggc aattaggggg ctgggaacat
     5401 cacccatcat ttaccgacaa aagccgtttt aactcctgct ttaaaacctc cggatctggc
     5461 aaataaagtt gatatcttga cacaaaaagc tgattagaaa taccaccctt agcatactca
     5521 accaaaatct catctttatc tcgcgccaac acaatcccta tcggctcatt gtcatcctcc
     5581 atattctcct cagcccgaaa ataatttaga tacatattca tctgtccaat atcctgatga
     5641 ctcaccgctc gcgtcttcag atcgattaag acaaaacact tgagaatgcg atgataaaac
     5701 accaaatcca caaaaaaatg cgtgttgttt aacgtaattc ggtattgctg cccaataaat
     5761 gcaaacccct tgcccaactc aagtagaaac ttctccaact cagcaattaa acgattttct
     5821 aactcactct cacgataatt gcggtctggt aaatccaaaa actcgaacac ataaggatct
     5881 ttgaccacat cccgcgccga ctcgatccgt tgcccatctt tagccagtgc caaaatctcc
     5941 gcccgatcct tactcaacgc cacccgctca aacaaagccg aatccttctg gcgtttcaac
     6001 tcccgcacac tccagcgatc ttggatgcac tgctgctcat aaaatgaccg cgccaaatca
     6061 tcggaaatag ctaaaagctc cgtatagtgc gaccaactca atttgccaga cagtgtttgg
     6121 caattctcat agttcaaata aaacagacgc atatactgca aattagaacg gctaaaccct
     6181 ttaccatacg ccgctttcaa atctcgtgaa agcacctgta acaacttact gccatattct
     6241 gcccgttcct tgcccgcttg ctcaaactcg acaatataac gcccaatctg ccagtaagtt
     6301 tctactaaaa ctgaattaac ctgctcaaaa gaccgttgtt gccctaacgc caaacactca
     6361 ccaatgcgat ctaacaactg ctgatatgaa ccatcattaa ccgttaaatc actcataact
     6421 caaaccccaa cgagagccaa ctgcgtctga aatctccaaa cagttgtaat aatgtaatta
     6481 tgtaaataag tacaaatgta ataatgtaat ttattacatt agctttttag ctcctcctca
     6541 actagagcaa tcatatattc cttaatggtc atgccttttt tggtagcacg gattttaatc
     6601 tcctggtgct gtgcctctgt aacttcaaaa gtaactcgct tgcttttgcc gttgttaact
     6661 tcttctgtgc ctgtagtact aggcgtttca gcagcattgc gactttctac ccatgctgct
     6721 gcttcatctt ttacaggcgt tttctgaaca cctggcctta agcttagttt ttgtgatttt
     6781 ttaacttctg ccatgattcg tttcctctct tgcttctaat atctcctcaa caacagctcg
     6841 tacttcatca ctggcagcac ttttcggggc agcttccaaa acagtactcc caatgttcaa
     6901 acactcagca aatgctaccc tttgacttat ctgtgctttt aacactggaa aggggtattc
     6961 tcctaaaact tcagttactt ctcgcccaag agcagtgttc gctatctttc tgttgattag
     7021 aaatgctgct gttaggtctg gcttatatat agtggcctct tgtaccagtt ctactacctc
     7081 atgcaccgcc cacacatcca aaggagatgg ttgtacagga accagcacaa aatccactgc
     7141 catgattgct gatctggtta agtcagaaac tcttggcgca ccatcaatga aaacaaaatc
     7201 atacccctgc gctagtttgg gcaattcttt atgcaagata ggacgatcca aaccaataac
     7261 gctaaatgga gcctcaccat tacgtgctgc tgcccagtca cgggttgagc cttgaggatc
     7321 agtatcaaca agtaatactc gataccctat catcgaaaaa gcatgagcca aatggataga
     7381 tactgtggtc ttgccactgc cccctttttg attcaaaact gcaattttca taaatgtgtt
     7441 tttgtaaaaa tgtaattcat taagttatga caaattctag aaagtaataa taccaagctt
     7501 aatccctaaa ttacaaattt acaaatgcac aaaatagtat taatacaaat gtaattaatt
     7561 acaaaaatac atttataact agaaaatgac cacttttaca cggtctttgt atctcagtaa
     7621 tgtaaagcta aattaattac aagtttatct aatgtctgtt gtgatctcaa ggcaggcttg
     7681 tttgttatta gtagagtaga ttctctcaag aaacccaaat tacacggttg tcggctcaat
     7741 gccaaaaaag cgatcgcgtt agatcaatat atataggcag taattacacg taatggctct
     7801 ttcattttat gcactcgcag ttttgattcg gaaaaattct atataatcct accgtaaagg
     7861 gtgattatac ggaaacttcc tgtataataa atagttgggc gggggaaagg cagtacatga
     7921 gcagcaattc tgagagatgg cgacgtatgg ttcaagtgta agcggtactc gaacgtactt
     7981 tgttgttaaa acgcgattct ttcgctctct tcaatggaag ggagcttcgt aacatgtgca
     8041 tttcagcagg gaggttcgta tgaaaaactt tgttccaaca agtatcgcat tggcaggtct
     8101 tgtgatgctt gctgcgtccg cagcgcctat acacgcgcaa tgtgcatcgc cgaagtatgt
     8161 tagcggagtt tggaaggcga atgatggggg cacataccgc cttcatgcca tcggcagtac
     8221 catttggtgg gtcggaatga gcggtgacga cggtcgttcc tggacgaacg tgttcaaagg
     8281 tacgcgaaac ggcaatatca ttgatgggga ttgggccgac gttcgcggac aaccgtgggg
     8341 gcatggaacg ctcaagctac ggatctcggg tacaactttt atggagaaga tcgacggcac
     8401 tggcagcgga ttcggcggga ttcgttgggg ccgtggcgga tgtaacgaca cgcaaggcaa
     8461 cccggggtag tgtgaaggta gctgcaacca ggggcaaact caacagttta gtttatataa
     8521 attgataaat atgttttccg cccaagcagc gatcgctctt gttgggttaa tctgcggctg
     8581 aatgccaaaa aagcgatcac gccagatcaa tatatatagg ctctgattac acgtaatggc
     8641 tcaaaggtgg aataggcaaa agtatacttg aaaaaatctt tctcccccag cctccccagc
     8701 ttgcgaggct cgacctcgca cctttatttc gcggtcgtac ctattcccaa agctgataaa
     8761 ttcaaaccaa aattcaataa ttttacattt aatgggtggg tttattttaa tgcttttaaa
     8821 tcatgtaagg tacagagcaa acgaggcttt tcaggatcag tcaaatctag ctgctcccaa
     8881 gatattgtat agccactgtg gatcttgtgt gccccaaaaa ggtgtaagat tctcaaccca
     8941 attgtccaag cagatagtct ttctaacaca gcaatggttc cgatcagata ttcctgaata
     9001 acccattctt cacctcgttg ttcggcacgt acttcctcaa tgcgtccggc ggatttgcca
     9061 gttgaatcca atacttgttt gcccagtagc agttccagat gaatttcacg agttgccatg
     9121 ttctgcccct ggaattcgtc caataatgtg ttcccgaagc catttttcag aagccaatgc
     9181 cggagttgcg tcagcttcaa tctcgacttc aacatcgata ccaacatccc gcacttttga
     9241 ccacggaatt ctaaacggtt cactttgttt aggggtagtg ttacggaaag tgtcaccagg
     9301 ggcaaagtaa tgaataaagt accgaatttt attgaggagt tggaacttta tccactttga
     9361 ttctgcttca gtgggtaaaa agggttgaac agcacataaa aatcgctctt tagttccgac
     9421 tccatttttt atcggaactt tttagcaatg gtgagtgatc gctatcccca atcattataa
     9481 aagctgaaac gcttgtctgg tagtacttac acgctacttt gcccctggtg acagcttccg
     9541 taactttacc ccgtttagca ccccaactac tttgaattgc tgctacccac cttcctaaac
     9601 gtggatgaat ccgtcgtgca agagttgtca taccagtttc gatataagag agtcgtggcg
     9661 gttccccgtc gcgcaactct atgacaattc catcgacttt acccagctta tgctgatcgc
     9721 tgtcaactaa ttggttatcg agtacatctc taataatgtt cattttttat cacttccagt
     9781 tgattattga tgtatttaac gtaaacacgc agcagcgatc accctccaag aatttctagt
     9841 ggaatagcaa cgatcgcttt catcgaaagt aggataagcg tgtagctgtt accagagacg
     9901 tgctggggtt agccacgact aactaaggca tctgtgacga gcagcttttg gtaaaacagc
     9961 taggcgaatc catcctaacg gggttatttt tctagctaaa tttgtttaat tagactgaag
    10021 gcttattccg ctctctacgt tgcaatagaa gaggtaagct atatcagcaa actataggac
    10081 ggcatgagga cgaatcaaac gagcaatcga gaaagtaagt taaattcacg acagcgcctt
    10141 gttggaatca ggatcttgct agtagaggat gagccagata ttgcagacct gctcatcttc
    10201 gttctggaaa cagcaggtgc agaggtga
//
