LOCUS       NC_010628              20972 bp    DNA     circular CON 15-MAR-2024
DEFINITION  Nostoc punctiforme PCC 73102, complete sequence.
ACCESSION   NC_010628
VERSION     NC_010628.1
KEYWORDS    RefSeq.
SOURCE      Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)
  ORGANISM  Nostoc punctiforme PCC 73102
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae;
            Nostoc.
REFERENCE   1  (bases 1 to 8234322)
  AUTHORS   Ekman,M., Picossi,S., Campbell,E.L., Meeks,J.C. and Flores,E.
  TITLE     A Nostoc punctiforme sugar transporter necessary to establish a
            Cyanobacterium-plant symbiosis
  JOURNAL   Plant Physiol 161 (4), 1984-1992 (2013)
   PUBMED   23463784
REFERENCE   2  (bases 1 to 8234322)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L.,
            Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E.,
            Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M.
            and Atlas,R.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2008) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000020025.1-RS_2024_03_15
            Annotation Date                   :: 03/15/2024 03:30:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,702
            CDSs (total)                      :: 7,602
            Genes (coding)                    :: 7,314
            CDSs (with protein)               :: 7,314
            Genes (RNA)                       :: 100
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 133 of 288
            Pseudo Genes (incomplete)         :: 166 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 85 of 288
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:28:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4829311
            Orig. end                         :: 4850283
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP001037.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 2351359
            Source DNA available from John C. Meeks (jcMeeks@ucdavis.edu)
            Bacteria available from ATCC: ATCC 29133
            Contacts: John C. Meeks (jcMeeks@ucdavis.edu)
            David Bruce (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LLNL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            NOTE: This genome has an insertion element which pops out under
            certain growth conditions. When it pops out, gene Npun_R0414 is put
            together in one piece.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20972
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene"
                     /region_number="16"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..20972
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20972
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10972](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..10972)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(186..1106)
                     /gene="lipA"
                     /locus_tag="NPUN_RS19335"
                     /old_locus_tag="Npun_R3828"
     CDS             complement(186..1106)
                     /EC_number="2.8.1.8"
                     /GO_function="GO:0016992 - lipoate synthase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="lipA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006197450.1"
                     /locus_tag="NPUN_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3828"
                     /product="lipoyl synthase"
                     /protein_id="WP_041565513.1"
                     /transl_table=11
                     /translation="MISSQQAELKSEIAAMPSWLRRPIGKASEISTVQRIIKQRQIHTI
                     CEEGRCPNRGECYAQKTATFLLMGPTCTRSCAFCQVDKGHAPMPLDLEEPQKVAQAVQL
                     LGLRYVVLTSVARDDLPDQGAGHFVETIATIRQLNPETQIEVLTPDFWGGAGVGESGQR
                     QRIAMIVKAKPACFNHNIETVRRLTGPVRRGAKYDRSLLVLAMVKEIDSTIPTKSGLML
                     GHGETLDEVVEAMADLRAVGCDRLTIGQYMRPSLEHLPVQKYWTPEEFDQLGRLAWEMG
                     FNHVRSGPLVRSSYHAGEEGVGNEE"
     gene            1439..2092
                     /locus_tag="NPUN_RS19340"
                     /old_locus_tag="Npun_F3829"
     CDS             1439..2092
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006197448.1"
                     /locus_tag="NPUN_RS19340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3829"
                     /product="response regulator"
                     /protein_id="WP_012410184.1"
                     /transl_table=11
                     /translation="MASNKILVIDDTTVVRVKVREMLPPGNFEVLEAKDGLEGLNFILQ
                     EKLSLIMLDFLLPKMSGWEVFQKVQADPELRKIPLVIMSGRKEEVTEKITEPFEYFEFL
                     GKPFDQKQLINAIKLAMTKAKLPRPELVAVGAGVAVKNSTVATSSVATATLATPTVASS
                     AMPTAASNATPTAGGVSEAEINALNEKIVKMQAEIDGLKKQLTQVVTFIKQKIK"
     misc_feature    complement(2575..2588)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 22.69"
                     /tool="antismash"
     gene            2698..8313
                     /locus_tag="NPUN_RS19345"
                     /old_locus_tag="Npun_F3830"
     CDS             2698..8313
                     /EC_number="2.7.11.1"
                     /EC_number="2.7.13.3"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_functions="regulatory (smcogs) SMCOG1030:
                     serine/threonine protein kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015137196.1"
                     /locus_tag="NPUN_RS19345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3830"
                     /product="ATP-binding sensor histidine kinase"
                     /protein_id="WP_012410185.1"
                     /sec_met_domain="Pkinase (E-value: 4.7e-38, bitscore:
                     129.7, seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLVVLDTLYSFPGYHITEQIYSGSKTLVYRGIREQDQKPVVLKLM
                     RNEYPTFAEIAQFRNQYIITKNLDIPGILKTYSLESYRNSYILVMEDFGGISLQDWRIE
                     NKENKENYLSLNEFFNIAIKIASTLEGLHRDRIIHKDIKPANILINPTTDEVKIIDFSI
                     ATLLPREIQFLTNPNILEGTLAYISPEQTGRMNRGIDYRTDFYSLGITFFQLLTGQLPF
                     TTKDPMELVYSHIAKQPLKASRINSKIPAILSEIINKLMAKNAEDRYQSALGLKHDLEV
                     CQKQWQETGNIAPFEVGMRDISDRFVIPEKLYGRQGEVETLLAAFKRVTEGAIEMILVA
                     GFSGIGKTAVVNEVHKPIVRQRSYFIKGKFDQFQRDIPLSGLVQAFRDLIGQLLSETDA
                     QIQQWKAKILRELGTQTQVIIDVIPELEQIVGKQAPVTDLSGSAAQNRFNLLFQKFIQI
                     FTTKEHPLVIFIDDLQWADTASLKIIQLLMCESISSSLAENAEHTHESQGGLLLIGSYR
                     DNEVFKVDPLYLTLQEIEKAGAAINTITLSPLNQGDLNHLIADTLHCAEVVAVPLTQMV
                     FAKTKGNPFFSVQFLKSLHDDGLIILNFDVGHWQYDISKVKELALTDDVVEFIALQIGK
                     LPIYTQKVLKLAACIGNQFDLKSLAIVNEKSVVDTASDLWQALLDGLILPQTENYNIFA
                     KENTNQEFIVHGIESIEFSSSNLQLPKYKFVHDRVQQAAYSLIPEEQKKPIHLKIGLLL
                     LNNIPVAEQEEKIFELVNQFNIAVEFISPQAKRDELAQMNLIAGRKALASTAYPTALKY
                     LTTGIQLLADDSWETKHYLTLALYETATEAAYLAGNFEQMEQLAEVVLVRKPLLEKVKV
                     YEVKIQAYGAQNQALEAVNTALTFLKLLGVEFPEHPSQSDIQLAMAELTSNLNERLIGD
                     LINLPEMIEAQPLAIMRILSTATPITYAVAPKLFPLIVLKQIELSLKYGNAPLSAFGYS
                     NYGLILSGLVGDIESGYQFGKLSGSLVDKLNAKDVKAKIMLGFNTTIRHWKEHTRQVLK
                     PLMEGYYAALEIGDLEYAAYYLHSYSYSSYFIGKELIGLEREIENYSNVVRQINQKTVF
                     NWIKIHQQSVLNLLGNAVNPCYLMGEAYNEKKMLPFYIDTNDIMGIFYLYVSKLHLCYL
                     FQEYPLALENATLAEKYLQGGIGQQVTPIFYLYDSLARLAAYPDASDYEQNLILNKVQA
                     NQQKMECWAHHAPMNYLHKYYIVEAEKYRISGQYLEAMEFYDRAIFLTKEHEYIHEEAL
                     AQELAAKFYLEWGKHKISQTYLTDAYYSYVRWGALAKVDDLAKRYPQLLAPIFQQEKVS
                     IKSSQESVSLNNTSISSLSTLSNQQTVIGSKTSISDSLDLAAFIKASQALSGEIELERL
                     LSTLMEVVMENAGASKCALILSEGDNLALTVTAVCSSSNFEYTYTEFPSTSLESSYDVP
                     ITLINYVKRSREILVIDDTTSVSFLASDNYIISEEPKSLLSIPLVNQGKLIGILYLENH
                     LTTGAFTRDRVEVLKLLTTQAAISLENAILYKNLAKAKESLEEYNHTLEEKVQERTQEL
                     NDKNNCLQETLQELQRTQIQLIQSEKMSSLGQMVAGIAHEINNPINFIHGNINHAGEYV
                     QDLLDLLVVYQQEYPHSSPLVEEKYEEIDIDFLSEDLPKILDSMKVGSSRIRNIVLSLR
                     NFSRLDESEMKPVDIHEGIDNSLMILQHRLKENSNFPEIEVIKQYGKLPEVICYPGQLN
                     QVFMNILSNAIDALEDSLSSASDFVGKIHIFTELTDSNTAIIRITDNGFGMTKAVQQKI
                     FDPFFTTKAVGSGTGLGLSISYQIIVDKHKGRLTCDSTLGKGTEFVIEIPMQQSDI"
     gene            complement(8436..9827)
                     /locus_tag="NPUN_RS19350"
                     /old_locus_tag="Npun_R3831"
     CDS             complement(8436..9827)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1034: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019491416.1"
                     /locus_tag="NPUN_RS19350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3831"
                     /product="cytochrome P450"
                     /protein_id="WP_012410186.1"
                     /sec_met_domain="p450 (E-value: 4.7e-76, bitscore: 255.0,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQLPNILKSPSLLQKLHWVSDPIGYMENAAQEYPDIFTGKIVGFG
                     DTVVFVNHPQAIQEILTNDRKKFTAVGELNGILKPLLGDNSVLMLESDRHKRQRQLVTP
                     SFHGERMQAYGQLICNVSKKIFNQLPLNKPFVARNLTKEISLQVILQSIFGFYEGEKIQ
                     KLRQLLPLLLELFESPLSSSLFLFSFLQQDLGAWSPWGNFLRVREKIDQFLYTEIAECQ
                     QQADPERIDILSLLISCRDEAGQPMTDQELRDQLITLILAGYDTTATAMAWGLYWIHKQ
                     PLVCEKLLQELDTLGDSPDPMSISRLPYLTAVCNETLRIHPVTMFSFPRVVQEPLELLG
                     HSLEPGTILLPSIYLTHHRENLYPQSKQFKPERFIERQFSPYEFLPFGGGVRRCMGEAL
                     ALFEIKLALATIVSHYHLALVDQRPEQPQRRGFNLAPGSGVKMVMTDQRARKESLINMT
                     TTPLS"
     gene            complement(10001..10972)
                     /locus_tag="NPUN_RS19355"
                     /old_locus_tag="Npun_R3832"
     CDS             complement(10001..10972)
                     /EC_number="4.2.3.7"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T1TS"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052: Terpene synthase/cyclase metal-binding domain
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410187.1"
                     /locus_tag="NPUN_RS19355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3832"
                     /product="germacrene-A synthase"
                     /protein_id="WP_012410187.1"
                     /sec_met_domain="T1TS (E-value: 3e-82, bitscore: 276.0,
                     seeds: 237, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNQLLCPGLYCPFPSQTNKYVDVLEEYSLEWVLRFNLLANESAYK
                     RFCKSKFFFLAASAYPDSKFEELKITHDWLSWVFIWDDQCDLSELKKQPEVLNNFHQRY
                     LEILNGAELTSQDTLFSHALIDLRKRTLQRASIKWFNYFISYLEDYFYGCVQEATNRAK
                     GIVPDLDTYIMIRRSSVGVYAVLALSEFCNQFIIPDVLRNHHLVKKLELITTDIIAWSN
                     DIFSASREIASGDVHNLIFVLHYHKKISLEKAIEQVVKIHNEEVHSLIKVESSLSFFSE
                     ELDVEITKYISGMHSWIRGNLDWCYESYRYHNLERLELTEFK"
     misc_feature    11227..11238
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: medium, score: 18.48"
                     /tool="antismash"
     gene            11577..12875
                     /locus_tag="NPUN_RS19360"
                     /old_locus_tag="Npun_F3833"
     CDS             11577..12875
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015211405.1"
                     /locus_tag="NPUN_RS19360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3833"
                     /product="response regulator"
                     /protein_id="WP_041566298.1"
                     /transl_table=11
                     /translation="MLNLPEDSLILVVDDTTTNLGIVFDILTNVGFKVVTENDGSRALK
                     QAEEQLPDLILLDVMMPGIDGFETCKRLKENSATYDIPVIFMTANSDTNSKVRGLNIGA
                     VDYITKPFNEEELLARIKTHLQLRNLTKTLEKRVAERTAALSKALKDLQESQLQLVQTE
                     KMSALGQLVAGVAHEINNPVSFIHGNLGHASVYFQDIANIINLYQQYYPNPVPEIQEEI
                     AGIDLKYMLSDLPNLISSMKEGVQRIRNISTSLRTFSRADSDRKVSCNIHDGIDSTIMI
                     LKHRLKASENRPDIQIIRDYENLPDLECFIGQLNQVFMNLLANAIDSLEESNIGRTYIE
                     IEANPNQIFIQTTLNENNNYILIRIKDNGVGMSADVQQKIFDYLFTTKPVGQGTGLGLS
                     IAHQIVVEKHGGTLEVNSELEHGSEFIIKLPIY"
     gene            12905..14056
                     /locus_tag="NPUN_RS19365"
                     /old_locus_tag="Npun_F3834"
     CDS             12905..14056
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130894.1"
                     /locus_tag="NPUN_RS19365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3834"
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="WP_012410189.1"
                     /transl_table=11
                     /translation="MFKTAVGHSNDPDSLSAIEEVLQQCVSSLGGDIPKAGILFSAIDF
                     EHALILQQIHQFFPGIELIGGTTDGEISSVLEFQQDSITLMLFCSDEVEINAGVGRKVS
                     VDPIAVTKQAVEQAKAKTTETPRLCLTHPESLTISGVSILHGLKLALGQNVPIFGGLAG
                     DQSRYQNTYQFFQTEVLSDSVPILLFSGTILFSHGVASGWHPIGQISQVTKVDKNILYE
                     IDGKPALDFYHHYLGLLPPSMEYPLAVFDQNRDNFYIRAPIAYNQDSGSITFFADIPDR
                     AVIQIAEACYEDILAASKASFMNALNNYPGAEPSAVLFFSCVARRQILGTRAKEEYQNT
                     KLCLTYHLPGCGFYSYGEIAPIDGMSQTQFHNETFVTLILGTR"
     gene            14063..15109
                     /locus_tag="NPUN_RS19370"
                     /old_locus_tag="Npun_F3835"
     CDS             14063..15109
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130895.1"
                     /locus_tag="NPUN_RS19370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3835"
                     /product="ATP-binding protein"
                     /protein_id="WP_012410190.1"
                     /transl_table=11
                     /translation="MEFLDYQQENKELKKANRILQKQLERSEVDRVKLEETNEKKEYLL
                     RKVIAELKEYQNNLEERSQELEMMLLNLQIMQNKMSSLGSLVADVAHEINNPVGFIVGN
                     LTPAQEYIQNLLHLIDLYQKTYPQASQEIQEIIKVMDLEYVREDLPKLISSMREGTNRI
                     SNISSSLRTFSRTDTEQKVMFNLHECIDNTLFILKYRLKDNKIRPAIQVVKNYDGEIPP
                     IPCFPGQLSQVFMNLLANAIDALDESNSGLSFDEIKESHNQIIIHTARNKDENHALIKI
                     QDNGVGISPDVQQKMFSHLFTTKPVGKGTGLGLSIAYQIIVQKHRGTLEVKSVLGKGSE
                     FITRIPIS"
     misc_feature    complement(15291..15305)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: medium, score: 24.8"
                     /tool="antismash"
     gene            15503..16045
                     /locus_tag="NPUN_RS19375"
                     /old_locus_tag="Npun_F3836"
     CDS             15503..16045
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015116274.1"
                     /locus_tag="NPUN_RS19375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3836"
                     /product="methanogen output domain 1-containing protein"
                     /protein_id="WP_012410191.1"
                     /transl_table=11
                     /translation="MTNALNHSIATLNVSLERDIFLRTLIRELSGTLQDVVGLEEASGF
                     ISVVGERMATQLDQDYKSALEVSKLSRKQVADVLIDLKKRIQGDFYVVEQDDEKIVFGN
                     RVCPFGEKVLNRPAMCMMTSNVFGTIAANNLGYAKVELQQTIAQGASGCRVIVYLKFTE
                     EAEDAEGREYFRGLESV"
     gene            16130..17500
                     /locus_tag="NPUN_RS19380"
                     /old_locus_tag="Npun_F3837"
     CDS             16130..17500
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015211403.1"
                     /locus_tag="NPUN_RS19380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3837"
                     /product="ATP-binding protein"
                     /protein_id="WP_012410192.1"
                     /transl_table=11
                     /translation="MTPEQFLEFARVLPEPLLLVSGEGQLLATNQPVADMLGLRRQELR
                     GKMLFELVTQSTDDVVKYLQACSSSRAMVIGSLTLRKNDGQTLICRSQGAVIQPWSPES
                     SSLILLRLENRTLASSNFVLLNQKIDELAKEVQRRKQAEEALWKANQELEIRVEERTTA
                     LQETLNELQLTQTQLIQAEKMSSLGQMVAGIAHEINNPVSFIYGNLHHAYKYTHDLLKL
                     VQIYQQLCPNTPLEIQQELEEIDLDFLIQDITKLFQSMTVGTERIQEIVKSLRNFSRLD
                     EAELKQVNIHEGIDSALMILEHRLQARHEYPEIKVIKKYSQLPNVTCYPGQLNQVFMNI
                     LANAIDALEALAVNSQTTDNQKLTNNNPQIQIKTEVINEKWIAVSIADNGLGIDEQVRS
                     KVFDPFFTTKTVGKGTGIGLSVSYQIIVEKHGGQLSCFSVPGKGAEFVIKIPVNSVS"
     gene            complement(17833..19437)
                     /locus_tag="NPUN_RS19385"
                     /old_locus_tag="Npun_R3838"
     CDS             complement(17833..19437)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410193.1"
                     /locus_tag="NPUN_RS19385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3838"
                     /product="NAD(P)H-quinone oxidoreductase subunit N"
                     /protein_id="WP_012410193.1"
                     /transl_table=11
                     /translation="MDFANIASQLNAGTILPEGIVILTLLGVLIVDLILGRTSARWIGY
                     LAIAGLFTSIVALYFQWDNPNPISFTGGFNGDDLSIVFRGIIALSAAVTILMSIRYVDQ
                     SGTPLAEFIAILLSATLGGMFLSGASELVMIFISLETLSISSYLLTGYTKRDPRSNEAA
                     LKYLLIGASSTAVFLYGVSLLYGLSGGQTELSAIANGIATAKVGQSLGLVIALVFAIAG
                     IGFKISAAPFHQWTPDVYEGAPTPVIAFLSVGSKAAGFALAIRLLTTAFPLVADEWRFV
                     FTALAVLSMILGNVVALAQTSMKRMLAYSSIAQAGFVMIGLIAGTQAGYASMIFYLLVY
                     LFMNLCGFTCIILFSLRTGTDQIAEYSGLYQKDPLLTLGLSIALLSLGGIPPLAGFFGK
                     IYLFWAGWQAGLYWLVLLGLVTTVVSIYYYIRVVKMMVVKEPHEMSDAVKNYPQVRWDL
                     PGLRPLQVGLVITLIATSLAGILSNPLFTLANNSISNTPFLQATINSHVEAQNLLLLPK
                     LDSVSQSQPSVDSTAKI"
     gene            19441..19620
                     /locus_tag="NPUN_RS19390"
     CDS             19441..19620
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="NPUN_RS19390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_041565514.1"
                     /transl_table=11
                     /translation="MYRFWRLDIKANSVHSSKFSNPRTSSLNKSLTAWVQIAIAYGILF
                     AFAPTSLAYQQVSQ"
     gene            complement(19781..20053)
                     /locus_tag="NPUN_RS19395"
                     /old_locus_tag="Npun_R3839"
     CDS             complement(19781..20053)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017655616.1"
                     /locus_tag="NPUN_RS19395"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3839"
                     /product="hypothetical protein"
                     /protein_id="WP_012410194.1"
                     /transl_table=11
                     /translation="MNIVKLILLACPAFLVSMLLIVNPVHASSLKSLYATQVVTVASTQ
                     QISDLVTVNLIQASNPIIDRVGCNCANCVQSRFQSLQGKLPSVGF"
ORIGIN
        1 caaactggcg taccaaatac ccaaattctt taagaatatt tgtgtgaatt tggaaattgg
       61 gcattgggga atggggaatg gggcattggg cacttgtact gagcgaagcc gaagtattgg
      121 gcattagtta ttttttccct gctccccctg cctcccctgc ctcccctgcc tcccctgctt
      181 catctctact cctcatttcc cactccctct tctccagcat gataggaact gcgaaccaga
      241 ggcccagaac ggacatggtt gaatcccatt tcccatgcta atctgccgag ttgatcgaat
      301 tcctctggag tccaatattt ttggactggg agatgttcta aagaaggacg catatactgc
      361 ccgatagtca agcgatcgca ccccacagcc cttaaatcag ccattgcttc gacgacttca
      421 tcaagtgttt cgccatgtcc cagcatcaaa cctgatttgg taggaattgt cgaatcgatt
      481 tctttaacca tagcaagtac caaaagcgag cgatcgtatt tggctccccg gcgcactggc
      541 ccagttaacc gccgcactgt ctcgatattg tgattgaaac aagctggttt ggcttttaca
      601 atcatcgcta tccgttggcg ttgacctgat tctcctacac ctgcaccgcc ccaaaaatct
      661 ggggtcagca cctcaatttg agtctctgga ttcaactggc ggatagtagc gatcgtctcc
      721 acaaagtgac ctgcgccctg atcgggcaag tcatcacggg cgacagaagt cagcactaca
      781 taacgcaatc ctaaaagctg tactgcctgt gcaacctttt ggggttcctc taaatcaaga
      841 ggcatgggtg catgaccttt atctacttga caaaaagcac aagagcgtgt gcaagtaggc
      901 cccattagca agaaagttgc agttttttgg gcatagcact ctccccggtt gggacagcga
      961 ccttcttcgc aaattgtgtg gatttggcgc tgcttaataa tgcgttgtac ggtagagatt
     1021 tcactggctt taccgatagg gcgacgtaac cagctaggca ttgccgcaat ttcagacttt
     1081 agttcggctt gttgtgacga aatcatagac atccaagcaa gatacggata taaagtagtg
     1141 gtctttcaat ggacaaatga tagacaagaa tagacgcagg aaacggtctt aatatctccg
     1201 tgtctccgtg tccttatatg ctttatgcct tagaacaaag ctaaagcctt aaatatcacc
     1261 atgacataaa tcgaaaagag cgccagcaaa agttttacaa aacacctcta ggaatgtcat
     1321 ttataccgaa atatactatc ccccaattgc tagaaatttt gtatatagtg ggaggattct
     1381 caaggacaat cggcaaagcc gctatttaca cttaaagttt ctgttagagc aaacagtcgt
     1441 ggcaagcaac aagattttag ttatcgatga cactacagtt gtcagggtaa aagtacgaga
     1501 aatgttgcct ccgggcaatt ttgaggtact ggaagcaaaa gacggtttag aaggactaaa
     1561 ttttattctt caggaaaaac tcagcctgat tatgctggat ttcctgctgc ctaaaatgag
     1621 tggctgggaa gttttccaga aagtccaagc cgatccggaa ttaaggaaaa ttcctttggt
     1681 gatcatgtct ggtcgcaagg aagaggtaac tgagaaaatc acagaaccct ttgaatactt
     1741 tgaatttctg ggcaagcctt tcgatcagaa gcaactaatt aacgcaatta agttagctat
     1801 gactaaggcg aaactgccgc gcccagaatt agtagcagtc ggagcagggg ttgctgttaa
     1861 aaatagcaca gttgcaactt ctagtgttgc aactgctacg ttagcaactc ctactgttgc
     1921 cagtagtgcg atgcctacgg cggcaagcaa cgcaacccct actgccggag gagtctcgga
     1981 ggcagaaatt aatgcgttga acgagaaaat tgtcaaaatg caggctgaaa tcgatggctt
     2041 gaagaaacag ctaactcaag ttgttacctt tattaaacaa aaaatcaagt agcatttgtg
     2101 ttcattatta ttatcgcagc cgaaataacc cgttcatcat aatgggtgtc cgattaaaag
     2161 cgggtaaaaa tagttaattc aaaatactta tcttgacaaa ccatcgttga cagcaaaatt
     2221 caaaaatagc aaaaaactac atataggggt aattcatgaa ttgcctctac atgtaatttt
     2281 gtgtaagttt tataggtgca ttttcaaaaa taaatagtat tagttgttag tctactgaaa
     2341 cagccctaga cccactaaac ctgccgcagt ttgctcttaa cctaagaaaa acggcaggct
     2401 ttttattgct tagtctccca catatacaat ctgcatcaga gcttgctagt aaacctacag
     2461 atagaaaaca gtaataggga atatttacca gataaatcta gtaataatat tttgacaaac
     2521 ttatactttt ataccaaatt agtttattca aaattatatt gttcaagaaa atatatttat
     2581 taaaaaatta tttttattaa tagaaattaa acaaataaaa ttattatttc atgataatga
     2641 tagccttcgc acgggaatac taagacatag aaacaagaat tatgagcata ggctgcaatg
     2701 ttagtagtat tagatacatt atattcgttt ccaggctatc acatcaccga gcaaatatac
     2761 tcaggtagta aaactctagt ttatcggggc attagagaac aagaccaaaa accagtcgtt
     2821 ctcaaattaa tgcggaatga atatcctacg ttcgcggaaa tcgctcagtt ccgcaatcaa
     2881 tatatcatca ccaaaaacct cgatattcct ggcatactca aaacctatag cttagaaagc
     2941 taccgtaaca gctacatctt agtgatggaa gattttggtg gtatttccct gcaagattgg
     3001 cgaatagaga ataaggaaaa taaggaaaac tatctttctc tcaatgagtt tttcaatatt
     3061 gctattaaaa ttgcttctac ccttgagggg ctacatcgcg atcgcatcat tcacaaagat
     3121 atcaagcctg ccaacatcct aataaaccct accacagatg aagtaaaaat catcgacttt
     3181 agtattgcca ccctcctacc aagagaaatt caatttctca cgaatcccaa catcttagaa
     3241 ggtactctgg cttacatttc tccagaacaa actggaagga tgaaccgggg tatcgactat
     3301 cgcactgatt tttattccct tggtattacc ttctttcaac tcctcaccgg acagttaccc
     3361 ttcactacta aagatccgat ggagttagtt tactctcata tcgccaaaca accgctaaaa
     3421 gccagccgca ttaattctaa gattccagca attttgtctg aaattatcaa taagctgatg
     3481 gcgaaaaacg ccgaagaccg ctatcagagt gctttggggt taaagcatga cttagaggtg
     3541 tgccaaaagc agtggcaaga aacaggaaat attgcaccct ttgaagtagg aatgagggac
     3601 atctcagacc gtttcgtaat ccctgaaaaa ctctatggtc gtcaaggtga agttgaaacc
     3661 ctactcgctg cttttaagcg tgtaactgaa ggggcaatag aaatgatatt agttgcaggt
     3721 ttctctggca ttggcaaaac tgctgtggtc aatgaagttc acaaacctat tgtgcgacag
     3781 cgtagttact tcatcaaagg aaaatttgac cagtttcaac gtgacattcc cttatcagga
     3841 ttggtgcaag cttttagaga tttaatcggg caactactct cagaaactga tgcccaaatt
     3901 caacaatgga aagccaaaat cctgcgggaa ttgggtacac agactcaggt aattatcgat
     3961 gtaatccctg aactcgaaca aattgttggt aagcaagctc cggttacaga cctctctggc
     4021 agtgctgctc aaaatcgttt caatttattg tttcaaaaat ttattcaaat ctttacaact
     4081 aaagaacatc ccctagttat ctttattgat gacttgcaat gggcagatac agcatcttta
     4141 aaaataattc agttactaat gtgtgagagt atttcatctt ctttggcaga aaatgcagaa
     4201 cacacacatg agagtcaggg aggtttattg ttaattggtt cttatcgaga caatgaagtt
     4261 ttcaaagtag atccgcttta tttaacacta caggaaattg aaaaagccgg agcagctatt
     4321 aatacaatta ccttatcacc tttaaatcag ggtgatttaa atcatctcat tgctgatact
     4381 ctgcattgtg ctgaagtagt cgcagttccg ctcacccaga tggtgtttgc caaaaccaaa
     4441 ggcaacccat tcttttccgt ccaattcctc aagtctttac acgatgatgg gctaattata
     4501 ttaaactttg atgttggtca ttggcagtat gatatttcca aagttaaaga gttagctctc
     4561 acagatgatg tagtagaatt tattgcccta caaataggaa aattgccaat atatacccaa
     4621 aaggttctga aactagcggc ttgtattggt aatcaatttg acttaaagag tctagcgatc
     4681 gtcaacgaaa aatctgtagt agatacagcc tcggatttgt ggcaagcctt acttgatgga
     4741 ctaattttac cacagactga aaattacaat atctttgcga aagaaaatac taatcaagaa
     4801 ttcattgttc atggaataga atctatagaa ttttccagtt ctaacttaca gctacctaaa
     4861 tataaattcg tacacgatcg ggtgcagcaa gcagcttatt ctttgattcc tgaagaacaa
     4921 aaaaagccaa ttcacttaaa aattgggcta ctgttattaa acaatattcc agttgcagaa
     4981 caggaagaaa agatttttga gcttgtcaat cagtttaata ttgcagtgga attcatctcc
     5041 cctcaagcta aacgagatga actagcccag atgaatctaa ttgctggacg taaagcttta
     5101 gcatcaacag cctatcctac agcccttaag tatttaacta ctggtatcca actgctggca
     5161 gatgatagtt gggagacgaa acattatctt accctggctt tatatgagac tgcgacagaa
     5221 gcagcatatt tagctggcaa ctttgaacag atggaacaat tagcagaggt agtgttggta
     5281 cgtaaaccac tactagaaaa agtaaaagtt tatgaagtca aaattcaggc ttatggggcg
     5341 cagaaccaag cactggaggc cgtaaatact gccctaacct ttttgaaact tttgggagtg
     5401 gagtttcctg aacatccaag ccagtctgat attcagttag caatggcaga attaacgtca
     5461 aatctcaatg aaagactcat tggggactta attaatctgc cggaaatgat cgaagctcaa
     5521 cctctggcaa taatgcgtat actatcaact gcaaccccta ttacctatgc agttgctcct
     5581 aaactttttc cgctaattgt tctgaaacaa atcgagttat cgctcaaata tggtaatgct
     5641 cctttatctg cctttggcta tagtaattac gggttaatac tgagcggact agtcggagat
     5701 attgagtctg gttatcaatt tggcaaacta tctgggagtc tagtggataa gctgaatgct
     5761 aaagatgtta aagctaaaat tatgctggga tttaacacaa ccattcggca ttggaaagaa
     5821 catactagac aagtattaaa acctttaatg gaaggttact atgccgcact agaaatagga
     5881 gatttagaat atgcagctta ttatcttcac tcttattcct actcttcata ttttattggt
     5941 aaagaactca tagggttaga aagggagata gaaaattaca gcaatgttgt caggcaaatc
     6001 aaccaaaaaa cagtatttaa ctggattaaa atccaccaac agagtgtctt gaatttactg
     6061 ggaaatgcag tgaatccctg ttatttaatg ggtgaagctt acaacgagaa aaaaatgttg
     6121 ccgttttaca ttgacaccaa tgatataatg ggtattttct atttatatgt aagtaaacta
     6181 catctctgtt acctatttca ggaatatcct ttagccttgg aaaatgctac tttagcagaa
     6241 aagtatttac aaggtggaat aggacagcaa gtaactccta ttttctactt atatgattca
     6301 ctagcacgac tggctgcata ccctgatgca tcggattatg agcaaaattt aattctgaat
     6361 aaggttcaag caaatcagca aaaaatggag tgttgggcac atcatgctcc aatgaattat
     6421 ttacataaat attatattgt agaggccgaa aagtatcgga ttagtggtca atatcttgaa
     6481 gcaatggaat tttatgatcg cgcgatcttc cttaccaaag aacatgagta tattcatgaa
     6541 gaagcactcg ctcaagagct tgccgcaaaa ttttatctcg aatggggtaa acataaaatt
     6601 tcccaaacct atctaacaga tgcttattat agctacgttc gctggggagc gttagccaaa
     6661 gttgatgatt tggcaaaacg ctatcctcaa ttacttgctc ctatattcca gcaagaaaaa
     6721 gtaagtatca agtccagcca ggaaagcgtt tctttgaata acacatctat atcatcttta
     6781 tctactctga gtaatcagca aactgttatt ggctctaaga caagtatttc tgattctctg
     6841 gatttagccg cattcattaa agcatctcaa gcgctctctg gagaaatcga gcttgaacga
     6901 ctactttcta ctttgatgga agttgttatg gagaatgcag gagcctctaa atgcgcttta
     6961 attttgagcg aaggtgataa cttagcatta actgtcacag ctgtttgttc aagttcaaat
     7021 tttgagtata cctacactga atttccatca acttcccttg agtcgagcta cgatgttccc
     7081 attactttga ttaactacgt taaacgctcc agagagatac tggtaattga tgatacaaca
     7141 tctgtttctt ttctagcaag cgataactac attattagtg aagaacctaa gagtttgttg
     7201 tctatacctc ttgtaaatca aggtaaattg attgggattc tttacctaga aaatcatctg
     7261 acaacaggag cctttacccg cgatcgtgta gaagtactta aactcctcac cactcaagca
     7321 gcaatatccc tagagaatgc tatcctctac aaaaatttgg ccaaagctaa agaaagcttg
     7381 gaggaatata accatacttt agaagaaaaa gtccaggaaa gaacgcagga actcaacgat
     7441 aaaaataact gtttacaaga gactttacag gaactacagc gtacccaaat tcaattgatt
     7501 caaagtgaaa aaatgtcttc tctaggacaa atggttgcgg gcattgctca tgaaataaat
     7561 aatcctatta actttattca tggcaatatt aatcatgcgg gtgagtatgt ccaagacttg
     7621 cttgatttgc tagttgttta tcagcaggaa tatccccatt cttcgccttt agttgaggag
     7681 aaatatgagg agattgatat agattttcta tcagaagact tgccaaagat tctagattca
     7741 atgaaagtgg gtagttcgcg tatccgaaat atcgttttga gcttacgtaa tttttctcgc
     7801 ctagatgaat ctgagatgaa gcctgtagat attcatgagg gaatagacaa cagcttaatg
     7861 attttacaac accgacttaa agaaaatagc aattttcctg aaattgaagt catcaaacaa
     7921 tacggaaaat taccggaagt tatttgttac cccggtcagc tgaatcaggt gtttatgaat
     7981 atcctgagta atgcaatcga tgctttagaa gattcattat catcggcttc tgattttgta
     8041 ggaaaaatcc acatctttac tgaactaaca gattctaata cggcgataat tcgaattacc
     8101 gataatggtt ttgggatgac aaaagcggtg cagcagaaaa tatttgaccc attttttact
     8161 accaaggcgg taggaagtgg cacaggctta gggttgtcca ttagctatca gattattgtg
     8221 gacaaacaca agggtcgttt aacctgcgac tctacattag gaaagggaac tgagtttgtg
     8281 attgagatcc cgatgcaaca gtcagatatc tagactaagt aggtaagcaa gaatatttcc
     8341 aacaagattt gtagtaataa ctttagttat tattttctaa gcactaaatt tattactaca
     8401 aatttgctta cccatcaatt taacttgaaa accaactagg ataggggtgt agttgtcata
     8461 ttaattaaag attctttacg cgcacgttgg tcggtcatta ccatcttgac tccactgcct
     8521 ggggcaaggt taaaacctct acgctgaggt tgctctggcc gctgatcaac tagcgccaag
     8581 tgatagtgtg acacaatggt tgctaatgct agctttattt caaatagagc caaagcctcg
     8641 cccatacaac gacggacacc accgccaaag ggcaggaatt catagggaga aaattgacgt
     8701 tcaataaagc gttctggttt aaattgctta gattggggat ataaattctc gcgatgatga
     8761 gtaagataaa tactgggaag cagtatagtt ccaggctcta aagaatgtcc caataattcc
     8821 aggggttctt gcactaccct gggaaacgaa aacatcgtga cggggtgaat tcgcaaggtt
     8881 tcattacaga cagctgtaag atatggtagt cgggaaatgc tcataggatc tggggaatca
     8941 ccgagagtat ccagttcttg aagcagtttt tcacagacta aaggctgttt gtgaatccag
     9001 tacaatcccc aagccattgc tgttgcagta gtatcgtagc cagcaagtat aagggttatt
     9061 aactgatcgc gcaactcctg atctgtcatc ggttggcctg cttcatcccg acatgatatt
     9121 agtaaagaga gaatatcgat gcgttctgga tcggcttgtt gttgacactc ggcaatttca
     9181 gtgtagagaa attgatcaat tttctctcga acgcgcaaga aatttcccca aggactccaa
     9241 gctcctaaat cctgttgcaa gaatgagaaa aggaataaac tagaactaag aggtgactca
     9301 aaaagctcta acaacaatgg caatagttgc ctaagttttt gaattttttc tccctcataa
     9361 aagccaaaga tagactgtaa aatgacttgc agagatatct cttttgttag attacgagct
     9421 acaaagggct tatttagtgg taactggtta aagatttttt tagacacatt acagattaac
     9481 tgaccataag cttgcatccg ctctccatga aaagagggtg tcacgagttg tcgttggcgt
     9541 ttgtggcgat cgctctctag catcaggact gaattatccc ccaaaagagg ctttaaaatt
     9601 ccgttgagtt cgccgacagc tgtaaacttt ttgcgatcgt tagttaaaat ttcctgaatt
     9661 gcttgggggt ggttcacaaa gaccactgta tccccaaacc cgactatctt tccagtaaaa
     9721 atatcaggat attcctgggc tgcattttcc atatagccta tagggtcact aacccaatga
     9781 agcttttgta gtaaagaagg acttttgaga atattaggta gttgcatcta gttcttgttt
     9841 taaattgtag tgagctaatg gtcatttatt cttactacct taacttgtta aagtagcagc
     9901 ttaaatattg attacatcca acttgaaagc atgttaatta ctatttgtta caagttcact
     9961 gcttcactac tttggctagt caatctagag tggaagtttt ttatttgaac tctgttagct
    10021 ctagcctttc taaattatga tagcgataag actcgtaaca ccaatcgaga ttaccacgaa
    10081 tccaactatg catccctgat atatattttg taatttcaac atctaattct tctgaaaaaa
    10141 atgaaagaga tgattctact tttatcaaag aatgtacttc ctcattatga atttttacca
    10201 cttgttctat tgctttttct aaagagattt ttttatgata atgcagtaca aatattaaat
    10261 tgtgaacatc accactagct atttctctgg atgctgaaaa gatatcgtta gaccaggcaa
    10321 taatgtcagt tgtaattagt tccagctttt tgacaaggtg atgatttctt aaaacatcgg
    10381 gaatgatgaa ctgattacaa aactcagaca atgcgagaac agcataaaca cctacacttg
    10441 accttcgtat cataatataa gtgtctagat cgggtacaat tccctttgcg cggttggttg
    10501 cttcctgaac acacccataa aagtagtctt ccaagtagct gatgaagtaa ttgaaccatt
    10561 ttatactagc tctttggaga gttcgttttc gtaagtcaat taatgcatga ctgaatagtg
    10621 tatcctggct tgtaagttct gcaccgttaa gtatttctaa atatctttgg tgaaaattat
    10681 ttaatacttc aggttgcttt tttaactctg atagatcgca ttggtcatcc caaataaaca
    10741 cccaactcaa ccagtcatgt gtaatcttca attcttcaaa cttgctatca gggtaagcag
    10801 atgcagctaa gaaaaagaat ttggacttgc aaaaacgctt ataagctgat tcattagcca
    10861 ggaggttgaa gcgaagtacc cattcaagag agtactcttc taaaacatcg acatacttat
    10921 tagtctgaga tggaaatgga cagtacaaac cagggcaaag taattgattc ataacaatgt
    10981 attagtttaa agtaccgaaa aaatgggttg ccctaccaga cagacagtag ctcaatacta
    11041 ttctttaaag agcctttaaa aaacttttaa aaaatttata tattaaaacg cacaagtttc
    11101 gtttacgcca ctacatagta gtgtgtaaac acatataaat atttcaacag ctatctagat
    11161 actttcatta aaaaataaaa gtagccactt ctgtgtgtct tcattgagca atatggttga
    11221 ctaactgaaa aagttaactg atgggtatca acaacgtaac attagcacta catttataaa
    11281 aagacaaggg taataatgac taattttaga gcgatcgtct tcataaagga ggattttcaa
    11341 ctctagtgaa agtgcagatt tgtcgttgat gtccatgctg tagcggttgc atagagcaat
    11401 ttagcctgag aatgttgctg ttatcagagt ttttgggaac tatatggttg tcagaggata
    11461 tatttgccag tcagtctgca aaataagggt gacttagctg cgatcgcagg caatagtaaa
    11521 caataaataa ggagttaaat taatttaaat acatgaatta taagccatat ctacaaatgc
    11581 ttaacctccc agaagatagt ttaattttag tggtggatga taccactaca aatttaggaa
    11641 ttgtctttga catattaact aatgttggtt ttaaagttgt tacagaaaat gatggcagta
    11701 gggcacttaa gcaggctgaa gagcaattac ctgatttaat tttattagat gtaatgatgc
    11761 cgggaataga tggatttgaa acttgtaaaa gactgaaaga aaattcagct acttatgata
    11821 ttccagtaat ttttatgaca gctaactctg atactaatag taaagttagg ggtctaaata
    11881 taggggcggt tgattatatt actaaacctt ttaatgaaga agagttacta gctagaatta
    11941 aaacccatct acaattacga aatcttacaa aaactttaga gaaacgagtt gcagaaagga
    12001 cagcagcgtt gtctaaggca ttaaaagacc tacaagagtc tcaacttcag cttgtgcaga
    12061 cagaaaaaat gtctgctctt ggccaattag tagcaggagt tgctcatgaa attaataatc
    12121 cagttagttt cattcatgga aatctcggac atgcttcagt atattttcaa gacatagcta
    12181 atattattaa tctctatcag cagtactatc caaatccggt accagaaatt caagaggaaa
    12241 ttgcagggat agatttgaag tatatgcttt ccgatttacc taacttaatt tcttcaatga
    12301 aagagggtgt tcagcgcatt cgtaatatta gtactagtct gagaactttt tctcgtgcag
    12361 atagcgatcg caaagtttct tgcaacattc atgatggcat cgacagtaca atcatgattc
    12421 tcaaacaccg tttaaaagca tccgagaatc gacctgatat tcaaataatt agagattatg
    12481 agaatttacc agatttagaa tgctttatcg gacagcttaa tcaggtattt atgaatttat
    12541 tagctaatgc tattgattct ttagaggagt ctaatatagg acgaacttat attgaaattg
    12601 aagcaaatcc taatcaaatt tttattcaga ctactctcaa tgaaaacaat aattatattc
    12661 taattcggat taaagataat ggggtgggaa tgtcggctga tgtccagcaa aaaatcttcg
    12721 actatttatt taccactaag cctgttggtc aaggtacagg attaggatta tcaattgctc
    12781 atcaaattgt tgtcgaaaaa catggaggaa ctctggaggt aaattcagaa cttgaacacg
    12841 gttcagaatt tatcattaaa ctccctattt actaattata ctaagtaact ttatcggaat
    12901 ctttatgttt aaaacagcag taggccacag taacgatcca gattctctgt cagcaattga
    12961 agaagttctt cagcaatgtg tcagttccct tggaggagat ataccaaaag ctgggattct
    13021 tttttctgca attgactttg aacatgctct cattttgcag caaattcatc agttttttcc
    13081 gggaattgag ttaattggtg gaacaactga tggagaaatt tcttcagttt tagaatttca
    13141 gcaagactca atcaccttaa tgttattttg ctcagatgaa gttgaaatta atgcaggagt
    13201 tggacgtaag gtttcagtcg atccaattgc tgtaactaaa caagctgtag agcaagccaa
    13261 agcaaaaact actgaaactc caaggctatg tttaactcat ccagagagtc ttacaattag
    13321 tggtgtatct atattgcatg gcttaaaatt agctcttggt caaaatgtgc cgatattcgg
    13381 cggtttagca ggcgatcagt ccagatatca aaatacatat caattttttc aaacagaagt
    13441 attaagtgat tctgtaccaa ttctactttt ttcaggcaca atattgtttt ctcatggtgt
    13501 tgcaagtggt tggcatccta tcggtcaaat aagccaagtg actaaggtag ataagaacat
    13561 actctatgaa atagatggta aaccagcttt agatttttat catcattatc taggtttact
    13621 tcctccttca atggaatatc ctctagcagt atttgatcag aatagagaca atttttatat
    13681 aagagcgccg attgcttaca atcaggattc tggtagcatc accttttttg cagatattcc
    13741 cgatcgagct gtaattcaaa ttgcggaagc atgttatgaa gatattttgg cagcttctaa
    13801 agcatcattt atgaatgcct taaataatta tccaggtgca gagccaagtg ctgttttatt
    13861 tttttcatgt gtagctcgtc ggcaaatact tggtacgaga gcaaaagaag agtaccagaa
    13921 tacgaaactc tgtttgactt accatttacc tggttgtggg ttttattctt atggagaaat
    13981 tgctcctata gatggaatga gccagacaca attccacaat gaaacttttg taactttaat
    14041 tttgggaact cggtagaatt ttatggaatt tttggattac caacaagaaa ataaggaact
    14101 aaagaaagcg aatagaattc ttcaaaagca attagagcgt tctgaggtag accgggtaaa
    14161 actagaagag acaaatgaga aaaaagaata tttgctcaga aaagtaattg ctgaattaaa
    14221 ggaatatcaa aacaatctag aagaaagaag ccaagaacta gaaatgatgc ttcttaatct
    14281 tcagataatg cagaataaga tgtctagttt gggaagtttg gttgcagatg tagctcatga
    14341 aattaacaat ccagttggct ttatagtagg taatctgact ccagctcaag agtatattca
    14401 gaatttgtta catttgatcg acctatatca aaaaacctat cctcaagcat ctcaagaaat
    14461 tcaagaaatc atcaaagtaa tggatctaga gtatgtgcgt gaggatttac ctaaactaat
    14521 ttcatcaatg agagaaggca ctaatcgcat ttctaatatt agtagtagct tgcgaacttt
    14581 ttccagaaca gatacagaac aaaaagttat gtttaacctt catgaatgta ttgacaacac
    14641 tctttttatt ctcaagtatc ggttgaaaga taataagatt cgtcctgcta ttcaagtagt
    14701 taaaaattat gacggtgaga ttcctcctat accatgcttt ccaggacaat tgagtcaggt
    14761 atttatgaat ttgttggcaa atgcaattga tgctttagat gagtcgaatt cgggattgag
    14821 ttttgatgaa attaaggaaa gtcacaatca aattataatt catactgctc gaaacaaaga
    14881 tgagaatcac gcattgatta agattcaaga taatggtgta ggaatatcgc ctgatgttca
    14941 gcaaaaaatg tttagtcatt tattcacaac aaaacctgtg ggaaaaggaa caggattagg
    15001 attatcaatc gcttatcaaa taattgttca aaaacatagg ggaactctag aggtaaaatc
    15061 tgtgctagga aaaggttctg agtttatcac tagaattccc atctcttaaa atagtaaatc
    15121 tatcaattct tgaaattaag attatttaaa attaattttt aatatagaat caggcgattg
    15181 aaacgaataa tttgtggttc ataatgggta attaactacg ctttcagagc agtaaaattc
    15241 atttatttaa aatagaaaaa tttgatcctg tttgtaaata agctggttta tttgcaaagt
    15301 gagaattggt ttgaagctag aagagacttt gcaaaaccga gaactgtcgc aacgacaaga
    15361 tttagaaatt gcgatcgcta tcacaattta ttagaaacac aagcaagcac caacttcttt
    15421 aaagaaaggt atcaaccagt tactttatgg tcattttggg gaactctata actgtagttc
    15481 caagattgtg ttctcaaaat ttatgactaa tgcacttaat cattcgatag ccacgcttaa
    15541 tgtttcttta gaacgcgata tatttttacg tacattaatt agagaattat ctggcacttt
    15601 gcaggatgta gttggcttag aagaagcttc tggatttatt agtgtagttg gtgaaaggat
    15661 ggctacgcag cttgatcaag attacaaatc tgctctggaa gtctccaaac tttcccgtaa
    15721 gcaggtcgct gatgtcttga ttgatttaaa aaaacgaatc cagggcgatt tttatgttgt
    15781 tgagcaggat gatgaaaaaa ttgtctttgg caaccgcgtc tgcccatttg gggaaaaagt
    15841 gcttaatcgc cctgccatgt gtatgatgac ttcaaacgtc tttgggacga tcgcagctaa
    15901 taatctggga tatgcgaaag tagaattgca acagacaata gcacagggtg cttcgggatg
    15961 tagagttatt gtttacttaa aatttacaga agaggcagaa gatgcagaag gtcgagaata
    16021 ctttagggga ctagaatctg tgtaaactct tcctcactaa aataaccata ggatacaagc
    16081 ttgagtgcag atatgtaaat tagaatcgaa ccctgaaatt ggaagcccca tgactcccga
    16141 acaatttctt gaatttgcca gagttttacc agaacctttg ctcctggtga gtggagaggg
    16201 tcagttgcta gcgaccaatc aaccagtagc agatatgctg gggttacgcc gtcaggaact
    16261 gcggggaaag atgctctttg aacttgtaac tcagtctact gacgatgttg taaagtacct
    16321 gcaagcttgt tcaagtagta gagcaatggt tattggctcc ttgactttac gaaagaatga
    16381 tggacaaacc ttaatatgtc gtagtcaagg agccgttatt caaccttggt ctcctgaatc
    16441 ttcaagttta attctcttac gtttagaaaa tagaacttta gctagcagta attttgttct
    16501 cctaaaccag aaaatcgatg agttagcaaa agaagttcag agacgtaaac aagcggagga
    16561 agcgctgtgg aaagctaatc aagagttaga aattcgggtt gaggaacgta caactgcttt
    16621 acaagaaaca ctaaacgaac tacaacttac ccaaactcaa cttatccaag ctgagaaaat
    16681 gtctagttta ggtcagatgg ttgctggtat tgctcatgaa ataaataacc ccgtcagttt
    16741 tatttatgga aacctccacc acgcctataa atacactcat gatttactga aattggtgca
    16801 aatctatcaa caactttgtc ccaatactcc tctagaaatc caacaggagc tagaagaaat
    16861 agacttagat tttctgattc aagatataac taaactcttc cagtcaatga cagtaggaac
    16921 agagcgaatt caggaaattg ttaaatcact acgtaacttt tccagactgg atgaagcaga
    16981 gcttaaacaa gtcaatatac atgaaggaat tgatagtgct ttaatgattt tggagcatcg
    17041 actgcaagct aggcatgagt acccagaaat caaagtgatc aaaaaatata gtcagctacc
    17101 taatgtaact tgctatcctg gtcaacttaa ccaagtgttt atgaatattc ttgctaatgc
    17161 gatcgatgca ctggaagcgc tggcggtcaa ttcgcaaaca actgacaatc aaaagctgac
    17221 aaataataat ccccaaattc aaatcaaaac tgaggtaatc aatgaaaaat ggattgcggt
    17281 tagtattgct gataatggtt tgggaataga tgaacaagtt cgctcaaagg tattcgatcc
    17341 atttttcact accaaaaccg tgggtaaagg cacaggaata gggctatctg taagctatca
    17401 aattatagtt gaaaaacatg gcggacaact gagctgtttt tcggttccag gaaaaggtgc
    17461 agaatttgtg atcaagatac ctgttaactc agtctcataa aattttagga cttacgtaaa
    17521 agaattaccc ctacaatttt gcgtaagttc tataggtgca tcttcataca gaattcaata
    17581 ctcctcgaag caacacattc ttgggtaaag acgcgattca tcgcgtctct acagatggtc
    17641 tatttgtcgc attctttttt caaattggta ttaaaggttt tttcttttcc ccgccccttc
    17701 ccaccttacc cttttcccca aaacccgacg acaagtattg atacagaatt ggtattaata
    17761 gatatgaaaa acgcctgtct gctaattagc aaacagacgt atctttgagt agaaagttat
    17821 attgagaatc ggttaaatct tagcggtaga atcaactgag ggttgagact gactaaccga
    17881 atctaacttt ggtaaaagca gtagattttg tgcttctaca tgactgttga ttgttgcttg
    17941 caaaaatggc gtattggaga tggaattgtt agccaatgta aacagtggat ttgacaaaat
    18001 ccctgctagg gaagtggcaa ttaatgttat caccagcccg acttgcaaag gccttagtcc
    18061 aggtaaatcc caacgcactt gtggataatt cttcaccgcg tcggacattt catggggttc
    18121 tttgactacc atcatcttaa ctacgcgaat gtagtagtag atggagacga cggtggtaac
    18181 taagcccagt aagactaacc aataaagtcc tgcttgccaa ccagcccaga ataagtaaat
    18241 cttgccgaaa aacccagcca atggtggaat accacccaag gaaagcagag caatactcaa
    18301 ccccaatgtt aggagtgggt ctttttgata taacccagag tattcggcaa tctggtcggt
    18361 tcctgtccgc agtgagaaca ggatgatgca ggtaaaaccg cacaggttca tgaacaagta
    18421 aaccagtagg taaaatatca tactggcata ccctgcttgt gtcccagcaa tcaagccaat
    18481 catcacaaac ccggcttggg caatggatga ataagctagc attcgtttca tgctggtttg
    18541 ggctagggcg actacgttac ccaaaatcat gctgagaacg gctagagcag tgaagacaaa
    18601 tctccactcg tctgcaacaa gagggaaggc tgttgtcagc aagcggatgg ctagggcaaa
    18661 cccagctgct ttggaaccaa cagataaaaa ggcgatgact ggggtgggag cgccttcata
    18721 aacgtctggt gtccactggt ggaaaggtgc agcggagatt ttaaagccaa tacctgcgat
    18781 cgcaaaaact agagcaatca ctaaacctaa agattgtccc actttcgctg tggcaatgcc
    18841 atttgcgatc gcacttaatt cagtttgtcc tccagataat ccatacagca gtgatacacc
    18901 gtacaaaaat actgctgtac tggaagctcc aattaacaag tatttcagcg ccgcttcatt
    18961 ggagcggggg tcacgcttgg tataacctgt cagcaagtaa gaggaaatac tcagggtttc
    19021 tagggagatg aaaatcatca ccaactcact agccccggat aaaaacatcc ctcctaaagt
    19081 cgcactcagc aaaatagcga tgaattcggc taaaggagtg ccgctttgat cgacgtagcg
    19141 aattgacatc agtatagtca cagccgcaga caaggcaatg atgccgcgaa agacgatact
    19201 caggtcatca ccattaaagc caccggtaaa agagatggga ttaggattgt cccattgaaa
    19261 atatagggcg acaatggaag taaataaacc tgcgatcgct agatatccaa tccagcgtgc
    19321 ggatgtacgc cccaaaatca aatcaacaat caaaaccccc aagagggtga gaataacaat
    19381 cccctctggt aaaatcgttc cagcatttaa ctgggatgca atattagcaa aatccatgag
    19441 atgtataggt tttggcgatt agacattaag gctaactctg ttcactctag caagttttct
    19501 aatccccgga ctagctcttt gaacaagagc ttaacagctt gggtgcaaat tgcgatcgcc
    19561 tacggcatac tgttcgcgtt cgcaccaact tcactagctt accagcaggt ttctcagtag
    19621 ttagggattc acctaaatac tttcctactt ggtttttaag gtatgacctt aattaaataa
    19681 tgatgccgca gctgcgatcg ctatgtacaa aaagctaatt aataattaat aagggcacag
    19741 gattgctgtg cccctacaag attcataact tttaacttta ttaaaaaccc actgatggca
    19801 gcttgccttg tagcgattgg aatctagact gaacacagtt tgcacaattg caaccaactc
    19861 gatcaataat cggattggat gcttgaatca agtttactgt cactagatcg gaaatttgtt
    19921 gtgtagatgc tacagtaaca acttgtgtag catatagaga tttgaggctg gatgcgtgta
    19981 ctggattaac tatcagtagc atggatacca gaaatgcggg acaagcaagt aaaatcagtt
    20041 tcactatgtt cataatttac acaaaaatac ctttggtgat acagcataac caataaattg
    20101 acgaggttca actttaatta ctgagtatta tttaactacg cccaaatcct tgacctcgta
    20161 aaacctttga ccaaataacc aattcgcctt gctcaccacc tgcggccagt aacttgcctt
    20221 ggggatgcca tgctaaggtc gaaaaacctc ctgagacacc tgtgataatt tgagatactt
    20281 ccttggcttg gttccacaaa cacaaccagc catcagcagc agcagaagcg agaaggaaac
    20341 tttttggtgc aaaggcgatc gcattgatcg tacctacatg atttgttaat actcgtgctt
    20401 cccaacctaa agtatcatcc tctagtttct cccacaccac aataccttca acgctggaag
    20461 atgccaggat tggcgcacct actttagtca tagcttctga ccatgccaat tggcgaattt
    20521 ttccagggaa accacgcatt acccaagggt cgggattgtt ccattccaaa acagtgacac
    20581 tacgatccat gttgccagaa gccaggaatt taccatcggg cgaccaagcc atagctaaac
    20641 tgacagtagt catatctagg ctgtatggtt cttcatccca gttttgacta tgccaaatct
    20701 ttactccctt ataaccacta atggctaagt attgtccgtc aatgcgccaa tctataccca
    20761 atactgaaga gttgtcgaaa ttcagcgtca cgacaatttc atgagtatct acatcccaga
    20821 cttggacgta acgccccaaa ctaaaagcca gttggttact ggtgtaattc caagctagct
    20881 tgtcaaccca tgcgggggca ttttccaagg tggcgattaa ttcagtattc tgccaaattt
    20941 ttacttgtcc atcctgtccg ccaacagcta aa
//
