LOCUS       NC_010628              20933 bp    DNA     circular CON 15-MAR-2024
DEFINITION  Nostoc punctiforme PCC 73102, complete sequence.
ACCESSION   NC_010628
VERSION     NC_010628.1
KEYWORDS    RefSeq.
SOURCE      Nostoc punctiforme PCC 73102 (Nostoc punctiforme ATCC 29133)
  ORGANISM  Nostoc punctiforme PCC 73102
            Bacteria; Cyanobacteriota; Cyanophyceae; Nostocales; Nostocaceae;
            Nostoc.
REFERENCE   1  (bases 1 to 8234322)
  AUTHORS   Ekman,M., Picossi,S., Campbell,E.L., Meeks,J.C. and Flores,E.
  TITLE     A Nostoc punctiforme sugar transporter necessary to establish a
            Cyanobacterium-plant symbiosis
  JOURNAL   Plant Physiol 161 (4), 1984-1992 (2013)
   PUBMED   23463784
REFERENCE   2  (bases 1 to 8234322)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Glavina del Rio,T., Dalin,E.,
            Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M., Vergez,L.,
            Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N., Kim,E.,
            Meeks,J.C., Elhai,J., Campbell,E.L., Thiel,T., Longmire,J., Potts,M.
            and Atlas,R.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (07-APR-2008) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000020025.1-RS_2024_03_15
            Annotation Date                   :: 03/15/2024 03:30:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,702
            CDSs (total)                      :: 7,602
            Genes (coding)                    :: 7,314
            CDSs (with protein)               :: 7,314
            Genes (RNA)                       :: 100
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 288
            CDSs (without protein)            :: 288
            Pseudo Genes (ambiguous residues) :: 0 of 288
            Pseudo Genes (frameshifted)       :: 133 of 288
            Pseudo Genes (incomplete)         :: 166 of 288
            Pseudo Genes (internal stop)      :: 86 of 288
            Pseudo Genes (multiple problems)  :: 85 of 288
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:28:06
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 4744555
            Orig. end                         :: 4765488
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP001037.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 2351359
            Source DNA available from John C. Meeks (jcMeeks@ucdavis.edu)
            Bacteria available from ATCC: ATCC 29133
            Contacts: John C. Meeks (jcMeeks@ucdavis.edu)
            David Bruce (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LLNL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. it is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376)
            NOTE: This genome has an insertion element which pops out under
            certain growth conditions. When it pops out, gene Npun_R0414 is put
            together in one piece.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..20933
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="terpene-precursor"
                     /region_number="15"
                     /rules="(PT_FPPS_like and not PT_HPP)"
                     /tool="antismash"
     cand_cluster    1..20933
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PT_FPPS_like and not PT_HPP)"
                     /kind="single"
                     /product="terpene-precursor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20933
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="False"
                     /core_location="[10000:10933](+)"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..10933
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PT_FPPS_like and not PT_HPP)"
                     /neighbourhood="10000"
                     /product="terpene-precursor"
                     /protocluster_number="1"
     misc_feature    complement(1192..1208)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 24.05"
                     /tool="antismash"
     misc_feature    1192..1208
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 27.31"
                     /tool="antismash"
     misc_feature    complement(2375..2387)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 21.78"
                     /tool="antismash"
     misc_feature    2519..2534
                     /note="TFBS match to CopR, Copper-responsive regulator,
                     confidence: weak, score: 23.25"
                     /tool="antismash"
     gene            complement(2815..4416)
                     /locus_tag="NPUN_RS19010"
                     /old_locus_tag="Npun_R3764"
     CDS             complement(2815..4416)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017314724.1"
                     /locus_tag="NPUN_RS19010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3764"
                     /product="alkaline phosphatase"
                     /protein_id="WP_012410120.1"
                     /transl_table=11
                     /translation="MESHNQLRLNRRQFLLRSAITAGGIISTNFVSKSKVFAEAPAIIT
                     SDKMRPAIPYGVASGDISNESIVIWSRSDRPAKMLVEYSTSESFRNVQRVVGPNALQNS
                     DFTARLYLRNLPPDQQLFYRVIFQDLDYKGTYSAPVKGTFRTPPKSRRDIFFVWGGDTA
                     GQGWGINPDFGGMKIYETMRQLNPDFFIHSGDNIYADGPIQSEVKLDDGTIWKNITTPE
                     KSKVAETLTEFRGNYIYNLLDENIKRFNAEVPILAQWDDHETRNNWYPGQIILNDDRYT
                     VKDVNLLTQRARQAFLEYLPIGYTTNEPDKARIYRSFKHGPLLDIFMLDERTYRGPNSP
                     NNQPVPSKDTEFAGRIQIQWLKRQLLSSKATWKVIASDMPLGLIVRDGSTDFEAWANGD
                     GPALGRELELADLLRFIKNKNIQNVVWLTADVHYAAAHYYDPSKAQFTDFKPFWEFVAG
                     PLNSGTFGPNQLENTFGPQLIFQSLPPGTKANRPPSEGLQFFGGVKIDGNTKVMTVTLR
                     NLAGETVYSIDLPPEK"
     misc_feature    4467..4480
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.62"
                     /tool="antismash"
     gene            4919..6325
                     /locus_tag="NPUN_RS19015"
                     /old_locus_tag="Npun_F3765"
     CDS             4919..6325
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015216666.1"
                     /locus_tag="NPUN_RS19015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3765"
                     /product="ATP-binding protein"
                     /protein_id="WP_041566288.1"
                     /transl_table=11
                     /translation="MLTIGGTTNANESNLRLESTLQELLSWQVHIEVERPGNDLSILFN
                     QEPLLPGIILNNNQEYVGMISRRKFFEYMSHPYSLALFSTKPIKILYKFFQTEILAISE
                     DMSIVKATEITLQRPPQIAYEPILVKTTCGKYKLLDFHQLLLAYSQIHVLTLVQLQQVE
                     EQSRVAKAGFRDLQQNYTRLIQNEKMAALGQLVAGIAHEINNPVNFIAGNLVHAIEYSQ
                     NLLNLIRLYQQCYPEPEADIKTAIAQIELDFLTTDLPRLLTSMEVGTKRIEQIILSLRN
                     FSRLDESEKKLVDIHEGIDSTLTILQSRLQNNQIGANINIIKEYSDLPQVECYAGLLNQ
                     VFMNILANAIDAIEESLVTCTERSRSISHSSLVRDKGKIFIRTQLTDENQVIIRISDNG
                     PGIPENLQKQLFDPFFTTKPVGKGTGLGLSISYQIVVEKHGGHLQCISTFGKGTEFIIE
                     IPIHLNSELV"
     gene            complement(6391..7494)
                     /locus_tag="NPUN_RS19020"
                     /old_locus_tag="Npun_R3766"
     CDS             complement(6391..7494)
                     /GO_function="GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017653222.1"
                     /locus_tag="NPUN_RS19020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3766"
                     /product="AI-2E family transporter"
                     /protein_id="WP_012410122.1"
                     /transl_table=11
                     /translation="MQSANKLPRWLTIGLAFPIIILNGWLLIQVVQYFQPLISIIAAAI
                     LLAFVLNYPIQFLQEQGVKRNLAIAGVLLLTLVILVALGITLVPLIIQQLVELANILPS
                     WIDSGTQQLQTFQDWALSQQQLPINLSGLFTQVLERLSNQLQSFTGRILGFAVDTIGIV
                     LNVLLAVVLTVYLILNGERLWDGIFQWFPHNIGLRLRELLREDFHNYFIGQATLGAVLG
                     VTITLAFLTLRVPLALLFGIGIGLFSLFPFGTGFGIAIVSLLVALQNFWLGVEVLGIAV
                     AIDQVNSNFVAPRILGNLTGLNPVWVVISLLLGAKLGGVLGLLIAIPIASFIKDTTDSW
                     RAGEFNKIDDIVSEPITVTSETAGTYS"
     gene            complement(7709..8143)
                     /locus_tag="NPUN_RS19025"
                     /old_locus_tag="Npun_R3767"
     CDS             complement(7709..8143)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207144.1"
                     /locus_tag="NPUN_RS19025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3767"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_012410123.1"
                     /transl_table=11
                     /translation="MKNQPVHVAIAILYQENKFLMQLRDNIPGILYPGYWALFGGHIEP
                     GETPNVAVKREILEEIGYELPPFVEFGCYTDERVVRHIFHAPLLVELNQLVLNEGWDMG
                     LLTPEDIHQGNCYSQNAREVRPLGNTHRQIMLDFMERNQS"
     gene            complement(8168..8656)
                     /locus_tag="NPUN_RS19030"
                     /old_locus_tag="Npun_R3768"
     CDS             complement(8168..8656)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015207143.1"
                     /locus_tag="NPUN_RS19030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3768"
                     /product="hypothetical protein"
                     /protein_id="WP_012410124.1"
                     /transl_table=11
                     /translation="MGTDLIKLATKQAVKQKFAQSVAFMQQTRNLSLSFLSGHNIVSYR
                     LGLIFLLVVGLCSCGSLTSSGLNGTNFKIGSNVTPIREIKPEQDNQATVYIQGQVEKQA
                     PLMKQWAYQINDSTGKIWVITNQKNLAKGAQVVIKGKIRYQSIPLGGKELGEVYLEEE"
     gene            8907..9785
                     /gene="folD"
                     /locus_tag="NPUN_RS19035"
                     /old_locus_tag="Npun_F3769"
     CDS             8907..9785
                     /GO_function="GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /gene="folD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410125.1"
                     /locus_tag="NPUN_RS19035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3769"
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD"
                     /protein_id="WP_012410125.1"
                     /transl_table=11
                     /translation="METKTAKLLDGKAIAAKIQQELSVAITQLQPEIGRPPGLAVLMVG
                     DNPASAAYVRNKEKACAKVGIASFGKHFPAETTFRELEKVIAALNDDERVDGILVQLPL
                     PDHLDAVALLHQIDPDKDADGLHPVNLGRLVRGEIGLRSCTPDGVMRLLQEYEIPLQGK
                     QAVVVGRSILVGKPMALMLLEADATVTIAHSRSHDLKSITQNADILIAAAGRPGLITAD
                     MVKPGAVVVDVGMNRVTDASGKSRLIGDVDFESTAGVAGFITPVPGGVGPMTVAILLQN
                     TFTSYSRAAKK"
     gene            10001..10933
                     /gene="crtE"
                     /locus_tag="NPUN_RS19040"
                     /old_locus_tag="Npun_F3770"
     CDS             10001..10933
                     /EC_number="2.5.1.1"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /GO_process="GO:0033386 - geranylgeranyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="crtE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene-precursor: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994390.1"
                     /locus_tag="NPUN_RS19040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3770"
                     /product="geranylgeranyl diphosphate synthase CrtE"
                     /protein_id="WP_012410126.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 3.6e-61, bitscore:
                     205.4, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVATDNVQKTPPEEATFNLVAYLKERQKLCDNALDRAIPIIYPEK
                     IYEAMRYSLLAGGKRVRPILCLATCEMMGGTIEMAMPTACAVEMIHTMSLIHDDLPAMD
                     NDDYRRGKLTNHKVYGEDVAILAGDGLLALAFELVALKTPANVNRELTLQVIVRLGRAL
                     GAAGLVGGQVVDLECEGKSDISLETLNFIHRHKTSALLEACVVSGGIIAGASTEDVQRL
                     TRYAENIGLAFQIIDDILDITSTKEQLGKTAGKDQKAKKVTYPSLWGLEESRLKAQELV
                     KVACAELEPFGDRAKPLQAIAHFITSRNN"
     gene            11199..11660
                     /locus_tag="NPUN_RS19045"
                     /old_locus_tag="Npun_F3771"
     CDS             11199..11660
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460306.1"
                     /locus_tag="NPUN_RS19045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_F3771"
                     /product="divergent PAP2 family protein"
                     /protein_id="WP_012410127.1"
                     /transl_table=11
                     /translation="MQDIGNILDNRVLLVALVACLIAQALKLVIEIVKNRKLNIRVLVT
                     TGGMPSAHSALVTALAAGVGQTLGWASPDFAVAMIFAIIVMYDAAGVRQAAGKQARILN
                     QMIDELFHEKPDFSQDRLKELLGHTPVQVIAGAALGITIYWLARFAYLN"
     gene            complement(11772..12422)
                     /locus_tag="NPUN_RS19050"
                     /old_locus_tag="Npun_R3772"
     CDS             complement(11772..12422)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015128909.1"
                     /locus_tag="NPUN_RS19050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3772"
                     /product="hypothetical protein"
                     /protein_id="WP_012410128.1"
                     /transl_table=11
                     /translation="MHRFQTLPSVLGLAIAGLIGGMSSVFAQQAIPLCQPPTSGEYLLL
                     VVSPTADNQKQLRSALPPELKTITCKYLNETVTRIGGFKKIDDANRWARYVSNIVGLSA
                     IITTRPTTADVQPQLQQQPPQQTVNYNPQALGEGYAVLVDYYNRPELVNSVQQAVGGNV
                     GFVSYGQRPYLLAVYTTNQSEAYNTLQKLNNGGFVASIVDSRKVILLRSTVRL"
     misc_feature    12459..12472
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: medium, score: 23.38"
                     /tool="antismash"
     gene            complement(12709..13038)
                     /locus_tag="NPUN_RS19055"
                     /old_locus_tag="Npun_R3773"
     CDS             complement(12709..13038)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875706.1"
                     /locus_tag="NPUN_RS19055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3773"
                     /product="MgPME-cyclase complex family protein"
                     /protein_id="WP_012410129.1"
                     /transl_table=11
                     /translation="MQTYYYVLASQRFLLQEEPIHEVIKERTRHYHEQEKQIDFWLVEQ
                     PAFLEAPQFAQIKAKCPQPSVAIISTNPQFITWLKLRLEYVITGEFQAPSETIPDALAS
                     LATVS"
     gene            complement(13104..13823)
                     /locus_tag="NPUN_RS19060"
                     /old_locus_tag="Npun_R3774"
     CDS             complement(13104..13823)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF07366"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011319464.1"
                     /locus_tag="NPUN_RS19060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3774"
                     /product="hypothetical protein"
                     /protein_id="WP_012410130.1"
                     /sec_met_domain="PF07366 (E-value: 5.2e-06, bitscore: 24.9,
                     seeds: 29, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSATQSNNLPLWVQDRDKVIAESTDVEWRYQTPPDYSRSKENLAK
                     ESIHNHLEGTLEAIVQNLVRTFEMEVSFKANPQQWLSIVNEQFRVSTNGGVEYTAADLS
                     AQGTYNLFMADSEHYKASEESFESSAKVFHTTFPQGFPWEVLEVFSGPPNVTFKWRHWG
                     HFNGEYKGHAPTGETIEIIGMSIAKVTDDLKVISLEHYFDNNLFLEKLTFGGKQTNAQK
                     PGSACPFSSWFKKSRKS"
     gene            complement(13954..14679)
                     /locus_tag="NPUN_RS19065"
                     /old_locus_tag="Npun_R3775"
     CDS             complement(13954..14679)
                     /EC_number="2.6.99.2"
                     /GO_function="GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA]"
                     /GO_process="GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012410131.1"
                     /locus_tag="NPUN_RS19065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3775"
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="WP_012410131.1"
                     /transl_table=11
                     /translation="MPTLGVNIDHIATIRQARRTVEPDPVAAAVLAELAGADGITVHLR
                     EDRRHIQDRDVRILRQTVRSHLNLEMAATEEMLAIALDIKPDYVTLVPEKREEVTTEGG
                     LDIIGQIARIGEIVDKLQSASIPVSLFIDAEPAQIEASVKVQAQFIELHTGQYAEAKDE
                     TNRHRELAILAKGCQQAIQAGLRVNAGHGLTYWNVYPVAALPGMEELNIGHTIISRAAL
                     VGIERAVREMKQAIRGNGA"
     gene            complement(14787..15128)
                     /locus_tag="NPUN_RS19070"
                     /old_locus_tag="Npun_R3776"
     CDS             complement(14787..15128)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016868321.1"
                     /locus_tag="NPUN_RS19070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3776"
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="WP_012410132.1"
                     /transl_table=11
                     /translation="MARLYADEMFPQKVSELLRTMGHDVLTVQEAGNANLGIPDDEVLA
                     FAIADNRTVITLNRQDFIKLHRSSPKHLGIIVCTNDTDRFRMATRINEAINQEESLIGN
                     LIRIVRPTS"
     gene            complement(15132..15458)
                     /locus_tag="NPUN_RS19075"
                     /old_locus_tag="Npun_R3777"
     CDS             complement(15132..15458)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015130397.1"
                     /locus_tag="NPUN_RS19075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Npun_R3777"
                     /product="DUF433 domain-containing protein"
                     /protein_id="WP_012410133.1"
                     /transl_table=11
                     /translation="MTLQELENQILALLPTEKAAIIQSLTQTINIGAKGITKTPGICGG
                     EACIAGTRIAIWLLVEARRLGITEVQLLQDYPHIGAADLVNAWIYADAYPEEIEAAIRA
                     NEFA"
     misc_feature    complement(16489..16504)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 20.91"
                     /tool="antismash"
     misc_feature    17894..17905
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.59"
                     /tool="antismash"
     misc_feature    18236..18254
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: medium, score: 23.9"
                     /tool="antismash"
     misc_feature    complement(18905..18918)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.46"
                     /tool="antismash"
     misc_feature    complement(19804..19817)
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 21.07"
                     /tool="antismash"
ORIGIN
        1 caaattttcc aagggcgcgc tagatggatg gggtttgagc aactgccgtt aacttctgcc
       61 tctagaatgt tgcctttggc tgaaaatatg tggttacaag cagataacac tgtggagttg
      121 aaaagtgtta acaccgcaga catagaagat atcgattcct tgttaagtgg tttgtcccaa
      181 ctacaaactt acttcctcca ctgcattaat ctgctgcaac ggcaggagat ggatgcagaa
      241 ttactgagat ttcaaggcag ggaacgtctt gaaagccaag tgatgcaaga aacgttagca
      301 gaattagcat atgtattgcc acagccaaag tcagacagac taaataatag tttaggattc
      361 agtagtaatc ttgatgcaac tgacccaaac caagccttac taattgctgc tggtgctgtg
      421 ggacgagcat taggaattaa gatttgtccg ccagctgcct cagaagattc taatcgagtg
      481 caagatcctg tagaagcgat tgctcgtgct tcgcgtatcc gcgtgcggcg aattcagttg
      541 cgggacaact ggtggcaaaa ggattgcggg ccgatgttgg catttacgct tgccgataaa
      601 ctgccagtgg cactattacc agtgtctgat acacgttaca aactctacgt tcctggggaa
      661 cgaacgccta tctctgtcga tgccaacata gcagcagccc tagctaagac tgcctacgca
      721 ttctatcgac ctttacccga taaaaagctg aaaacttttg atttacttca gtttgccctc
      781 caaggacatt acaaagaact aattatagtt ttagtaatgg gaattaccat cagtctgtta
      841 ggaatgctga cacctcaagc tacagctatc ctaatcgaca aagcgatccc agatgctaat
      901 cgaggcttgt tgagccaaat tgctgttggt ttggtggctg ctgcctttgg cggaacgctg
      961 tttcaactag cccttgggtt tgccaccatt aggctggaaa cttttgccga ttcctccacc
     1021 caagccgcag tgtgggatcg attgttaaat ttgaaggttt ccttttttcg ctctttctca
     1081 gttggtgact tggattctag agtcacggcg attactcaaa ttcgccagcg actgggtaac
     1141 acagtcttaa aaacaatctt ctccagtttg ttctcactcc tgaatctggg attgttattt
     1201 tactacaatg tttccttagc tctcatagct tgtgcagtgg cacttgtaaa tattgccgtc
     1261 actatcatct ctggtactct tattgttcgt aaagttcgcc cattactaga gctgcaagga
     1321 caaatctttg gcgtgatggt gcagttaatt aatggagttg ccaaactccg aatcgccaaa
     1381 gctgaaaccc gcgcctttgc ctactggggt aaacagtata cgcatcaact gaaattaatg
     1441 ctgagtaccc aaggcattga agacagcctc gcggtaatca atcaagtatt acctgtgtta
     1501 accactgctg ccctcttctg gtttgctact agtttaattg gccaatccca aactcctgga
     1561 ggaggtggtt tatccactgg cactttctta gcattcaacg ttgcttttgg cacgtttatt
     1621 aacggagcca ctagcttaag cagcacagtt gtggaagttt tgcaagtttt acccttgtgg
     1681 caacgggccc aacctatttt gcaagctgaa ccggaaatta gttctgctca ggcagatccg
     1741 ggtaggctat cgggcaaatt agttgtagac cgtataactt tccgttatcg cgacgatggc
     1801 cccttgacgc tggatgatgt caacattcgt gctgaaccag gagaatttat tgctattgtc
     1861 ggcccttctg gcagcggtaa atcaacttta ttgaggttac ttttggggtt tgaattcccg
     1921 aaatctggca cgatttacta tgacgggcaa gacttaactt ctttggatgt ccatgctgta
     1981 cgccgtcaat tgggtgtggt actgcaaaat agtcggttga tgtcagcctc tattttcgag
     2041 aatattgcca gtggtgcctt agtgacgatg gacgaagctt gggaagcagc ccagatggca
     2101 ggttttgcag atgatgttgc agcaatgccg atgcagatgc atacaatcgt cagtgaagga
     2161 ggtagtaatc tttctggtgg acaacgacaa cggctgttga ttgctagggc gttggtgttg
     2221 aaaccccgga ttttgctatt cgatgaggcg actagtgctt tagataacag aacccaagca
     2281 attgtcagtg aaagtttaca acagctaaag gtaactaggg ttgcgatcgc tcatcgcctc
     2341 agtactattc aaaatgctga ccgaatttac gtacttgcaa atggcaaagt agtgcaacag
     2401 ggtagttttg ttgaactagg aaatcaatca gggctatttg cacaactcat gatgcaacag
     2461 ttaatttaac tgagtattga taagatagta ggtaactaaa ttaacttctc tttttgccat
     2521 ttataattgt acactgacca attaaatgac gttgagacaa attcgtgtca tcgagtcaaa
     2581 ccttacccac tttagacttg caaccattag tgttgatata ggttgtaagt tcccggtgct
     2641 acttctaagc ttaatgctct ttgagtgcaa gctttacacc ctcaatgaca ttaatttggc
     2701 atagcgacaa ataaaaagtc actgtacaaa aaaaacagca tagccagtag acaaagtaaa
     2761 aaattgcata attactccat ctaactggct aaatatcgcc acgcttttga tgatttattt
     2821 ttctggcggc aaatcaatac tgtaaacagt ctccccagcc aagttacgca atgttacagt
     2881 catcaccttt gtatttccat caattttcac tcctccaaag aattgcaaac cttcacttgg
     2941 gggacgattt gcttttgtac ctggaggaag gctttgaaat atcaattggg gaccaaaggt
     3001 attttctagt tggtttgggc caaatgtgcc agagttcaaa ggcccggcga caaactccca
     3061 aaaaggctta aagtctgtaa actgtgcttt gctggggtcg taatagtggg ctgctgcata
     3121 atgtacatca gcagttaacc aaacaacatt ctggatattc ttatttttga taaatcgtag
     3181 taaatcggca agttctagtt ctcttcctaa agccgggcca tctccgttag cccaagcttc
     3241 aaaatcagta ctaccgtctc taactatcaa tcccagtggc atatcactag caatcacttt
     3301 ccatgttgct tttgatgaca gcaattgcct ttttaaccat tgtatttgta ttctacccgc
     3361 aaattctgtg tctttacttg gtactggctg attattaggg ctattcggcc cccgataggt
     3421 acgctcatcc agcatgaaga tgtctaataa tggcccatgc ttaaaggagc gataaattct
     3481 cgctttgtct ggctcattgg ttgtataacc aataggcaga tattccaaaa atgcttgcct
     3541 tgctctttga gttagcaagt taacatcctt aactgtataa cggtcatcgt taaggataat
     3601 ttgtccagga taccaattgt ttcttgtttc gtggtcgtcc cactgtgcta atatcggaac
     3661 ttcagcgttg aaacgcttga tgttttcatc caacaaattg taaatatagt tgccacgaaa
     3721 ttctgtcaga gtttctgcta ctttggattt ttctggtgta gtgatgtttt tccagattgt
     3781 gccatcgtct agcttaactt ctgattgaat ggggccatca gcataaatat tatcgccaga
     3841 gtgaatgaaa aagtcgggat taagttgacg catggtttcg taaattttca tcccaccaaa
     3901 atcaggatta attccccatc cctgaccggc agtgtcgcca ccccaaacaa agaatatatc
     3961 tcgcctagat ttgggaggag ttcggaaagt acctttgaca ggagcgctgt aagtgccttt
     4021 ataatctaaa tcttggaaga ttacccgata aaataattgt tggtctggtg gcaggtttct
     4081 aagataaagt cgtgctgtaa agtcgctatt ttgtaaagca ttggggccga caactcgctg
     4141 cacatttcta aaagattcgc tggtagagta ttctacgagc attttggcgg ggcgatcgct
     4201 acggctccaa atgacaatac tttcgttaga aatatctccg ctagctacgc cataaggtat
     4261 ggcaggacgc attttatctg aggtgataat tgcaggcgct tcagcaaaaa cttttgattt
     4321 tgatacaaaa ttggtggaga taattccacc tgcggttata gctgaacgta gcagaaactg
     4381 gcgacggttt aacctcaatt ggttatgaga ttccatacta aataatggca aatttaaaaa
     4441 ctattacagg aaagctttta ccactttttt tataataaaa agtaaagatt aagttatcga
     4501 aaggttaata aaaagctttc aatgtaatta tttcatatta aatagaaaaa gaacattaat
     4561 aaaaaaggtt atattttaac cttagtatta agtgaatatt ttgatataat ttgcaactaa
     4621 aaatacgctt atagcgctta acatatccta taaaaaatgg tgcgttattg ctttaaacta
     4681 acgtactaaa ttaaaattta cttgataata gcctctaatg ataagccgct aaatgtctat
     4741 actctcatat aaagtgttgg attcgctgaa gtactctata ttcccacatc tacactgtga
     4801 atttcgtgta gcaatttggg aaagataagt ttgtaacgat tgatgaactt tgaaattata
     4861 gagacgggta tttataatgt tgccagaaat attacctcag cattcaacac atgagagtat
     4921 gctgactatt ggtggtacaa caaatgctaa tgagtcaaac ttgcgattag agtcaacttt
     4981 gcaagaatta ctctcttggc aagttcatat cgaagtagaa cgtcctggca atgatttatc
     5041 tatcctcttt aaccaagagc ctttattacc aggaattatc ctcaataata atcaagagta
     5101 tgtagggatg atttcgcggc ggaaattttt tgaatatatg agccaccctt acagtttggc
     5161 tttattttct acaaaaccaa ttaaaattct ttacaagttt ttccagacag agatattagc
     5221 catttctgag gatatgtcta ttgttaaagc tactgagata actttgcaac gaccacctca
     5281 aattgcctat gagccaattt tggttaaaac tacgtgtgga aaatataagc tgctcgattt
     5341 tcatcaatta ctcttagcct actcccaaat tcatgtatta acactcgttc agctacaaca
     5401 ggtagaggaa caatctagag ttgctaaagc aggctttcgc gatcttcagc aaaactatac
     5461 gcgattaatt cagaatgaaa aaatggctgc attaggacaa cttgttgctg gtattgcaca
     5521 cgaaattaac aatcctgtca actttattgc tgggaacctt gttcacgcca tcgaatatag
     5581 tcaaaatcta ctcaatctca ttagattgta ccaacaatgt tatcctgaac cagaagcgga
     5641 tattaaaact gcgatcgcac aaattgaact ggatttctta actaccgatc tccctcgtct
     5701 tctcacctct atggaggttg gtaccaagcg aatcgagcaa ataattcttt ctttacgaaa
     5761 tttttctcgt cttgatgaat ctgagaaaaa attagttgat atccatgaag gtattgatag
     5821 tacgctaaca atcttacaaa gtcgtcttca aaataaccaa ataggtgcaa acatcaacat
     5881 aattaaagaa tacagcgatc ttccccaagt agagtgctac gccgggttac tcaatcaggt
     5941 atttatgaat attttggcga atgctattga tgctatagaa gagtcattag tcacttgtac
     6001 tgagcgtagt cgaagtatta gtcattcgtc attggtaagg gataaaggaa aaatttttat
     6061 tcggactcaa ctcactgatg aaaatcaagt aattattcgt atttccgata atggcccggg
     6121 aattccagaa aatttgcaaa agcaattatt tgatcccttt tttactacta aacccgttgg
     6181 taaaggaact ggattaggtt tatctatcag ttatcagatt gttgtggaaa aacacggtgg
     6241 tcatctgcaa tgtatatcaa cttttggcaa agggactgag tttattattg aaattccaat
     6301 tcacctaaat tctgagcttg tttagaaaaa caaacaataa ccaaagaaga gacattctta
     6361 gtcagttctt cttcatttag atattgaatt ctaactataa gttcctgctg tttcgcttgt
     6421 tactgtaata ggttctgaca cgatatcatc tatcttatta aactctccag cccgccagct
     6481 atctgttgta tccttgataa aactggcaat agggattgca attaacaaac ctagcactcc
     6541 tcccaatttt gcccccaaca acaaagaaat caccacccac acgggattta aaccagtcaa
     6601 attaccaaga attcgaggtg caacaaaatt agaattaact tggtcgattg caacagctat
     6661 acctaagact tcaaccccta accaaaagtt ttgcaatgct accaaaaggc ttactatggc
     6721 aatgccgaat cctgtcccaa agggaaagag agaaaacaaa ccaatcccga tgccaaaaag
     6781 taaagccaag ggaactcgca gcgtcaaaaa tgccagtgta attgtcactc ctaatacggc
     6841 tcccaatgtt gcttgaccga taaagtaatt gtggaagtct tctcgcagta attctcgcaa
     6901 tcttaacccg atgttatggg gaaaccactg aaatattccg tcccaaagac gttcgccgtt
     6961 taatattaga tagacagtta acactaccgc cagcaacaca ttgagaacaa taccaatggt
     7021 atcgacggcg aaaccaagaa ttctgccagt gaaagactga agttggttag ataatcgttc
     7081 caaaacttgg gtaaataacc cacttaaatt aatgggaagc tgttgctgac ttaacgccca
     7141 atcctggaaa gtttggagtt gctgagtacc agaatcaatc caacttggca aaatattggc
     7201 tagctcaacg agttgttgga taattagggg aactaaggta atgcctaaag ccactaaaat
     7261 cactagagtt aaaagcaaca ctcctgcgat cgctaggtta cgttttaccc cttgctcttg
     7321 cagaaactgg attggatagt ttaagacaaa agccagtagg atggcggcgg caataatgct
     7381 gatcaagggt tgaaaatact gtacaacctg gatcaatagc caaccgttaa gaataataat
     7441 cggaaatgcc aatcctatag ttaaccatcg cggtagtttg tttgctgatt gcattagtga
     7501 tgactccaca agagttataa atttattttg gcttctaggg agcgggagtg gggaaagagg
     7561 caggggggca gggggcaggg ggcagggagc aggggagcag gggagatgag ggagacaagg
     7621 ggacaaggag ataaacaaac tcttaactcc taactcttaa ctcttaactc ttaactcagc
     7681 actcagcact cctaatgccc aattcccatt aagattgatt tctctccata aaatccaaca
     7741 taatttgccg atgcgtattc cccaaagggc gtacttcgcg ggcgttttgc gaataacagt
     7801 taccttggtg aatatcttct ggggtcaata atcccatatc ccagccttca tttaaaacca
     7861 gttgatttaa ttccaccaat agtggtgcat gaaagatatg acggacaact ctttcgtcgg
     7921 tataacagcc aaattccaca aagggcggta gctcatagcc gatttcttct aaaatttctc
     7981 gcttcactgc gacatttggc gtttcaccag gttcgatatg accgccaaat agcgcccagt
     8041 aaccagggta gagaataccg gggatgttgt cgcgtagttg cataagaaac ttgttttctt
     8101 gatagagaat tgcgatcgct acatgcaccg gttgattttt catatattat taatttttaa
     8161 ttgctgatta ctcttcttct agataaactt cccccaattc tttaccacct aaagggatgc
     8221 tttggtagcg aattttaccc ttaatcacca cttgcgctcc cttcgctaga tttttttgat
     8281 tggtgataac ccaaattttg ccagtcgagt cattaatttg ataagcccat tgcttcatca
     8341 ggggagcttg tttttccacc tgaccttgga tgtaaactgt agcttgattg tcttgttctg
     8401 gtttaatttc tcgaattgga gtgacattgc taccaatttt gaaattagtc ccattcaaac
     8461 cagatgaggt taaactacca caactacaaa gtcctaccac caataaaaaa atcaacccca
     8521 ggcggtagga aacaatatta tgcccggata agaacgaaag ggacaaattt cttgtttgtt
     8581 gcatgaaagc cactgattga gcaaattttt gcttcactgc ttgctttgtc gccagtttta
     8641 ttaggtcagt tcccatctat aatattcttt ctctgtggtt tcagaaacac gctctcaact
     8701 gagcaaaaaa tgttctgaat tttatcctgc aattctttat tcttcagaga aagccgttga
     8761 tctgagaaaa taagcattat ggattgactg tgagagaaac gctacgacat caagcagata
     8821 cacaataata gttaaaagtc aataagcaat atttttactc tagacttttg gataaaaagc
     8881 gaattaattt atagttggga attttcatgg aaacaaaaac tgccaaactc cttgatggta
     8941 aagcaatagc cgcaaaaatt cagcaagaac tttctgtagc cattacacaa ttacaaccag
     9001 aaattggacg accccctggt ttagcagtgt taatggttgg cgacaaccca gcgtcagccg
     9061 cttatgttcg taataaagaa aaagcctgtg caaaagttgg catcgcctct tttggtaagc
     9121 attttcctgc cgaaacgacc tttagggaat tagaaaaggt tattgctgca ctaaacgacg
     9181 atgaacgcgt ggatggcatt cttgtacagt tgcccttacc tgaccactta gatgctgtgg
     9241 ctctgctaca tcaaattgat cccgataaag atgctgatgg actgcaccca gttaacttag
     9301 ggcgactagt acggggagaa atcggtttac gcagctgcac cccggatggt gtgatgcggc
     9361 ttttgcaaga atatgagatt ccattgcaag gaaaacaggc agtagtggtg ggacggagta
     9421 ttttggtggg taaacctatg gcgctgatgc tactagaagc tgatgctaca gtgacgatcg
     9481 ctcactcgcg atcgcatgac ctcaaaagca tcacccaaaa tgctgatatt ctaattgcag
     9541 cagcaggtcg tcccggactc atcactgctg atatggtaaa accaggcgct gttgtggtag
     9601 atgtgggaat gaatcgtgtc accgatgcta gtggtaaaag tcgcttaatt ggcgatgttg
     9661 actttgaatc aactgctggt gtagcaggtt tcatcactcc cgttcctggt ggtgttggtc
     9721 cgatgactgt tgccatattg ttacaaaata cattcaccag ctattccaga gcagcaaaaa
     9781 aatgaaagag agtgatgagt tatgagttat gagttaaaag ttaagaatta ttaatcctaa
     9841 ctctcaactc ctaagtcctc tacagacgcg attaatcgcg tttcaacccc taactcctaa
     9901 ttcctaactc ataattatga actcttcatt ttttcagtcc cacagcacct taaaattgtg
     9961 acgtataaga caaaaatccc aaagtgtcag gaattgaaga atggtagcaa ctgataacgt
    10021 tcaaaagaca ccaccagagg aagccacatt taacttagta gcctatctca aagagcgaca
    10081 aaagctttgt gataatgctt tggatcgggc tatccccatc atttatccag aaaagattta
    10141 tgaggcgatg cgctactcgt tattagctgg cggcaagcgt gtacgtccca ttctttgcct
    10201 tgctacctgt gaaatgatgg gtggcactat cgaaatggct atgccaacgg cttgtgctgt
    10261 ggagatgatc cacacaatgt ctttgattca tgacgacctc ccagcgatgg ataatgacga
    10321 ttaccgtcgg ggaaagctga caaatcataa agtctatggc gaagatgtag cgattttggc
    10381 tggggacggc ttgttagcac ttgcttttga gttagttgcc cttaaaaccc ccgcaaacgt
    10441 taacagagag ctaaccttgc aggtcattgt tcgtcttggt cgggcgctgg gggcagctgg
    10501 tttggtcggt ggtcaagttg tcgatttaga gtgtgaaggg aaatcagata tttctctaga
    10561 aacgctaaat ttcattcata gacacaaaac ttccgccctt ttggaagctt gcgtagtttc
    10621 tggcggaatt atcgctgggg catcaactga agatgtacag cgactaaccc gttatgctga
    10681 aaatattggg ctagcattcc aaatcataga tgatattctg gatatcactt ctacaaaaga
    10741 gcaattaggt aaaacagctg gtaaagacca aaaagcgaag aaagttacct atcctagcct
    10801 ttggggactt gaagaatcgc gcttaaaagc ccaagagcta gttaaagtag cttgtgcaga
    10861 attagaacca tttggagaca gagccaagcc actccaagcg atcgctcatt ttattaccag
    10921 ccgcaataac tagcaacgct ttgtaggatt ctaaattctt ataccgcaag ggtttcattg
    10981 attgggaatg ggtggtttat ttacatgatg ctgtactacc gcccagcaga attgaaaatt
    11041 caaaattaaa aagcttactt aatcggcttt taatccattt tgaataattg atctgtttcc
    11101 gccgtgatgt actaggttaa ttttggattt tgggttgaat ctaaatgttt cctcaaccaa
    11161 caactgctgc taatatttac taacctaacc aaaataccat gcaggacata ggcaacattt
    11221 tagacaaccg ggtgctgctg gttgctctgg tcgcttgttt aattgctcaa gcactaaagc
    11281 tcgtaatcga gatcgtcaaa aatcgtaaac taaatatccg tgttttggtg acaaccggag
    11341 gtatgcccag tgcccattca gctttagtta cagctctcgc agctggtgta gggcaaacac
    11401 tgggttgggc atctcctgat tttgccgttg cgatgatttt cgccatcatc gtcatgtatg
    11461 atgcagccgg agttcgccaa gcagctggta agcaagctcg tatcctcaat caaatgattg
    11521 atgaattatt tcatgaaaaa ccagacttta gccaagaccg tctgaaagaa ttacttggac
    11581 atacaccagt tcaggtaata gctggggcgg ctttgggtat aaccatctat tggttagcta
    11641 ggtttgctta tttaaactag gacaatgaat ctttgtttga gcgagtattt ttaattggta
    11701 actggtaatt tgtcaactga caaattacca acttggtaga tcgcatcagt atttcagaaa
    11761 aattaatatc gctacaaccg cacagttgag cgcagcagaa tcaccttacg actatctact
    11821 atgctggcga caaaaccgcc attgttcagc ttctgcaatg tgttgtatgc ttcactttgg
    11881 ttggtggtat aaactgccaa caaataaggg cgttgtccat aagaaacaaa acctacatta
    11941 cctcctactg cttgctgtac actatttact agttcaggac gattgtaata atctactaac
    12001 actgcataac cctctcctaa tgcttgagga ttatagttaa ctgtttgctg aggtggttgt
    12061 tgctgtagtt gtggctgcac atctgccgtt gttggtcgag tggtgatgat agcagacaag
    12121 ccaacaatat tgcttacata tcttgcccat cgattggcat catcaatctt tttaaacccc
    12181 cctatccgcg tcacagtttc gttgagatat ttgcaggtaa tggttttcag ttcaggcggt
    12241 aaggcgctac gcaactgctt ttgattatca gctgtgggac tgactaccaa taaaagatac
    12301 tcacccgaag tcggtggttg acaaagagga atggcttgtt gggcaaaaac agaactcatg
    12361 ccaccaatca accctgcaat cgctaatcct aaaacacttg gtaaagtctg aaatctatgc
    12421 acaaaagtta gattatgtaa atcaaggtgt cttagaagat tttataagaa aaaagctgaa
    12481 aacctactta cttcagtgtg ggaatgaaag gaatgttaca aaacaatttt gttattacca
    12541 aataaaattg gggactggga actgagaact ggaaaagttg gggaagaagt aataaccaat
    12601 gcccattcca cattccccat acttcgactc ctttcgactt cgctacctcg gcttcgctcg
    12661 gcacaagtca aggcaagtcg ctcagtacaa gttccccatt ccccatttct aagatacagt
    12721 agccaacgat gccaaagcat ctggtattgt ctcagaagga gcctggaatt ctccagtaat
    12781 aacgtattct aaacgcagtt ttaaccaagt aataaattgg ggattagttg aaataattgc
    12841 tactgatggt tggggacact ttgccttgat ttgtgcaaat tgaggtgctt ccaagaaagc
    12901 tggttgttca accaaccaaa aatctatttg tttttcttgt tcgtggtagt gacgagtgcg
    12961 ttctttgata acttcgtgta tcggttcttc ttggagaaga aagcgttgac tagccaaaac
    13021 gtaatagtat gtttgcattt tcatcctttg cttgctgtgt gaatcatgta agggcacagt
    13081 acgacattct gtacccttat caactaactc ttacgggatt tcttgaacca agaactgaac
    13141 ggacaagcac ttcccggctt ttgggcattt gtttgtttgc caccaaatgt tagcttttct
    13201 aagaacagat tattgtcgaa gtagtgttct aaggaaataa ccttcaagtc atcggtaact
    13261 tttgcaatgc tcatgccgat aatttctatt gtctctccag taggtgcatg gcctttatat
    13321 tctccgttaa aatgtcccca atgccgccat ttgaatgtga catttggtgg ccctgagaaa
    13381 acttccagca cttcccaagg aaatccttgg ggaaatgttg tgtggaagac ttttgcggat
    13441 gattcaaagc tttcttctga agccttgtaa tgctctgaat cagccataaa tagattgtaa
    13501 gtaccttggg ctgataaatc tgctgcggta tactctactc cgccattggt actcactcga
    13561 aactgttcat tgacaataga taaccattgt tgcgggttag ctttgaaaga tacctccatc
    13621 tcgaaggttc gcactaagtt ttgcacgatc gcttccagtg taccttcaag gtgattatgg
    13681 atactttctt tggcaagatt ttctttagaa cgagaataat caggcggtgt ctgatagcgc
    13741 cactcgacat ctgtgctttc tgctatcact ttatctctat cctgtaccca aagtggcagg
    13801 ttgttagact gtgttgcgct catagaagtt tttgctgaag atttgatccc aatttctaga
    13861 tttcgcagtg acaacccctc tctttgggag tgctgagtac tgagtgctga gtaggagcag
    13921 aggaagaaga attagtaacc aatgcccaat gccctatgcc ccattccccc ttatagcttg
    13981 cttcatctcg cggactgccc tttctatacc tactaatgct gcccgactga tgatagtatg
    14041 accaatgttc agttcttcca tgcctggaag tgcagccacc ggatagacgt tccagtaggt
    14101 gagtccatga ccagcgttga ctcgcaatcc agcttgaatg gcttgttgac accctttagc
    14161 taatatggct aattctcggt ggcgatttgt ttcatcctta gcctcagcat attgtccggt
    14221 gtgcaattcg ataaactgcg cctgtacctt gacagatgct tcgatttgtg cgggttcggc
    14281 atcgataaat aaactaactg gaatgctagc gctttgcaat ttatcgacta tctcacctat
    14341 tctagcaatt tgaccaataa tatctagtcc gccttctgtt gtgacttctt cgcgcttttc
    14401 ggggactaaa gtcacgtaat ctggtttgat atcaagtgcg atcgctagca tttcttctgt
    14461 tgcggccatt tctaaattaa gatgcgatcg cactgtttgc cgcaatatac gcacatccct
    14521 gtcttggata tgtcgacgat cttcgcgcag atgcaccgta attccatcag cacccgctaa
    14581 ttctgccagc accgcagccg ccaccgggtc tggttctacc gtccgccgcg cttgccggat
    14641 ggtggcaatg tggtcaatgt taacgccaag tgtaggcacc ccaaatttct cccaaatcca
    14701 caatcctcag aacttgattt taccggaaat agtgggagtt ttgcttaaga agctcaaaac
    14761 aaattgaaga cgagcttcaa ttattttcaa ctggtagggc gtacaatgcg aatcaagtta
    14821 cctatcaatg attcctcttg attaattgct tcattaattc gagttgccat tcgaaaccta
    14881 tcagtatcat ttgtacaaac aataatacct aaatgtttag gtgagcttct atgcagtttg
    14941 ataaaatctt gacgatttag tgttattaca gttcgattat cggcgattgc aaaggctaac
    15001 acttcgtcat ctggaatgcc taaatttgcg ttaccagctt cctgtacagt taaaacatca
    15061 tgtcccattg ttcttaacag ttcactaact ttctgtggga acatctcatc agcgtataaa
    15121 cgcgccatga tttaagcaaa ctcattagca cgaatcgcag cttcaatttc ttcaggataa
    15181 gcatctgcat atatccaagc attaactaaa tcagccgcgc caatatgagg ataatcttgc
    15241 aaaagttgga cttcagtaat tcccaagcga cgtgcttcaa ctaatagcca aattgcaata
    15301 cgagttccag caatacaagc ttctccacca caaataccgg gtgttttcgt gatccctttt
    15361 gccccgatgt tgatggtttg agttaggctt tggataattg ctgctttttc tgttggaagt
    15421 agtgccagga tttgattttc tagttcttga agagtcataa cttatcttag ctcaattttt
    15481 attgtactta ttaacaaagt ttaggtgtta aatctccgtt acaaaacgta attgcgttag
    15541 caaaagcagg tttttgggca caatacttta aataaatagc taatttatga agagttaatg
    15601 aagtatgaat acagcagttg agccgattga caagcttttg aactatcaaa ttagcgaaca
    15661 actttataca ggctctagaa ccttagttta ccgtgcaatt cgagaagccg atcaactacc
    15721 ggttgtaatc aaactgctcc agcaggagta tcccaccttc aacgaactgc tgctgttccg
    15781 aaaccagtac acgatcgcta aaaatattga cctacccggt attgttcgtc cctatagtct
    15841 agaaccttat cgcaacagct atgttttggt gatggaggat tttggcggca tttccctcag
    15901 agattggctc aatcaaaaaa tggcgagcaa ccaatatact ttgacagagt tcttagatat
    15961 tgcgatcgct ctgagcaata ttcttgacgg actctatcgc catcgggtta tccacaaaga
    16021 tatcaagcct gccaacatcc tcatcaaccc ggaaagtcag caagtcaagt tgattgactt
    16081 tagtattgcc tctcttttac ccagagaaac ccaagaagtt cacagtccta atgtcttaga
    16141 aggtactctc gcctaccttt ccccggaaca aaccggacgg atgaatcgtg gaattgatta
    16201 tcgcagtgat tactattctc tcggcgttac cttctatgaa ttactgactg gacacttacc
    16261 ttttcaatcc aacgatccga tggaattggt tcactgtcac attgccaagc aaccagaaga
    16321 attaaaaggc agggagcagg cttgccatga gcttgtcctg agcgtagtcg ttggcgcagc
    16381 ctctcgtaga gaagggcgta gccgaacggg agcaggaggt aggagggaag aagaaattcc
    16441 tgaagttctt tgtaacattg tgatgaaatt gatggcgaaa aatgctgaag atcgctatca
    16501 gagtgcatta gggttgaaat ttgatttaga aaaatgtttg tctcaattgc aggaaatagg
    16561 ctcgattaaa tcctttaaat taggtgagcg ggatatttgc gatcgcttca tcatcccaga
    16621 aaaactttat ggtcgccaag gcgaagttga aagtttattg gatgcctttg agagagtcag
    16681 tcagggaagc actgaaatta tgttggtagc aggcttctgt ggtattggta aaactgctgt
    16741 ggttaacgaa gtccacaagc caattgtgcg acaacgaggt tacttcatta aaggtaaatt
    16801 tgaccaattc cagcgcaaca ttcctttctc tgcctttgtg caagcctttc gagacttaat
    16861 taggcaactg ctgaatgaaa ctgatgccca atttgagcag tggaagtgca aaattctctc
    16921 tgctttgggt gagaacggac aagtgatgat tgaggtgatt ccagaactgg aaagtatggt
    16981 tggtaaacag cctcctgtac cagaactctc tgggagtgcg gcacaaaatc gtttcaaatc
    17041 gctgtttttg aaattcactc aagtcttcac tgccgtagaa catccattag taatctttat
    17101 tgatgatttg caatgggcag attctgcttc tttaaagtta atgcacttat taatgagcga
    17161 agttgatatc ggctatctac tgttaattgg tgcttatcga gataacgaag ttaatccagt
    17221 acatccgcta atgctgactt tagaagagat tcagaagtta ggtgctacag ttaatacaat
    17281 taccttagct ctcctcgagc aaacacccgt caatcattta gttgcagata cattaagttg
    17341 ctccctaatg ttagccacac cgttaacaga gttgatattg agcaaaacca aaggtaatcc
    17401 tttcttcagt acccagtttc tcaaagcgct acaccaagat caaataatta catttaattt
    17461 tgacggtggt tattgggaat gtgatattgc tcaagtgcga tcgctcgccc ttactgatga
    17521 tgtagtagag tttatggcaa ttcagcttga aaaattgtca atagaaactc aagaagtttt
    17581 gaaattagcg gcttgtgtgg gaaaccaatt tgatttagcc accttagcga tcgtctatga
    17641 acagtctgag gcaacaacgc cagatgagtt atggaaagca cttcaagaag gatttattat
    17701 tcctagcagt gatatttaca aattctatca acagcaagca ttggtaaata gccacaatga
    17761 ctatggacaa ataaccagca gctacaaatt tcttcacgat cgcattcagc aagctgccta
    17821 ttcactaatt cctgacaatc aaaagcaagt tactcacctg cacattgggc gtttgctgca
    17881 acaagcgacg actgtaaatg aacgagatga gaaaattttt gcgatcgtca accaccttaa
    17941 tcaaggggtc actttgatta gcgatcggca ggagcaacag gaactcgcgc atttaaactt
    18001 ggcggctggg cataaagcca agggtgcaac agcttatgca gcagctttag actatttcaa
    18061 cacaggtatt gccatactaa cgagtaattg ctggcacagc caatacaatc tcgccctcat
    18121 cttgtatgaa ggagcaacag aatcagctta cctctgcacc gattttgaac agatggaaca
    18181 gttagctgag atagtactga atcatgccaa atcctggctg gatcgagtaa atatctacag
    18241 tattaaaatc caggcttgca tggcacaaca tcagcagcta gaagccttgc aattagcacg
    18301 agaaatccta aagcatctgg gagtgcattt gccggaacaa cccaccccag cagacatcga
    18361 acaggcactg aggcacaccc aaaccttgct caatggcagt tcaatcgaat ctttgttgga
    18421 attaccgcaa atagccgcac cggagaaaaa agcggcaatg gtgatgttat ccagcattat
    18481 ttctgcggct tatcaagctg ctcctgacct tttgcccctg gtgatctttg ccgaattcca
    18541 tttatcagta gaatacggca atgctccaga atcgactcat ggctatgtaa tgtatgggtt
    18601 gaccttggtt gccacattgg gagatattga cgcaggctat cggtttgggc aactggggat
    18661 gaatcttatg caacgcattc atgcccctaa actaactgct aaaaccattt ttgcctttaa
    18721 ctgcttcctt agacattggc aagaacctgc aaaatctacc ttggaaggat atttacaagc
    18781 ttatgccagt gggctagaaa gcggtgatat cgagtatgtc gctctcagcc tgatgtgtta
    18841 ttgctacaca gcttactggg gtggtcagga gctatctgcc ttaagacaaa caatagaagt
    18901 gcattgcaag gtcattcagc aattgcgcca ggatacctgt ttgcacacac aaagtattta
    18961 tcatcaggct gtagtcaatt tgttggaggt tgtgctggaa cctgagcggt tacttggcga
    19021 atactgccat gaggatgaaa taattcagct ttacgtcaaa acgaatcagg aagcaggact
    19081 atatcaggtt tatttcaata aacttttcct ttcctacctt ttccaagact atgaacaagc
    19141 gttagaaaac gccaaacttg cagaaccgta tttgggtgca atcacaggac tagtgatgct
    19201 tcctctgttt tacttctacg atgcactagt tcgattagcg gtttacccaa ctgccagtaa
    19261 gacagaccaa atcagaatta tagaacgggt gaggggccat caagaaaaac tcagcacttg
    19321 ggcggctcat gcaccaagta atcaggctca ccgccatgct ttagtaatcg cagaaaaatg
    19381 tcgagtcttg gggaccaaag ctgaggcaat tgagtattac gatcgcgcca ttgctaaagc
    19441 caaagaaaac gaatacctca atgaggaggc tcttgctaat gaactcacag ctaaatttta
    19501 tttagaatgg ggcaaagaac gcattgctca agaatatttg attaattctt actattgtta
    19561 cactcgctgg ggtgctaaag ccaaagttga taatttagaa caacgttatc cacaattgct
    19621 agctcccata ttacaacaga agcgttcacg aagtgactct gaaagagtat cgccactcac
    19681 ccacaatgat acgatcgcaa atattgacag cattactacc ttccactcaa gcattcataa
    19741 atcagcaaca tctaacagca atacgtcagt aatgctagat ttggcagcgg ttctcaaagc
    19801 ctctcagact ctttccagtg aaattgaact ggataaatta cttacgaagc tgctacaagt
    19861 ggtaattgaa aatgctggag ccgataagtg tgcgttacta ctgctcaaag aaggtagatt
    19921 gctagttgag gcgaccactg aagttggaca ccaatcaagg ttgttacagt ccatttttgt
    19981 agaagatagc gcagacattc cccatagcct aatttatgcg gttaaacgca gcctgcaacc
    20041 taccgtcatc cttgatgcaa cagtgtatcc gatgttaatg gtcgatccct acatcatgcg
    20101 tcagcaaccc aaaagcttgc tgtgtacacc gattctctat caaggcaaat tgttggggat
    20161 tttgtattta gaaaacaatc ttactactgg ggcatttacc agcgatcgcg tcgaaattct
    20221 taatctatta tgtactcaag ctgcaatctc cctagaaaac gcccgacttt atcagcaagc
    20281 acaagatact ctagaaaacc ttggacagac tgaacaattc ttacggttaa ttatcgacaa
    20341 tatccctcag tctgttttct ggaaagaccg caattgtgtt tatttaggct gtaactataa
    20401 ctttgctcaa acttcaggtg tagaattgcc agaaaatgtt attggtaaaa cagactacga
    20461 tctttcttgg actcgtgagg aatcaaattg gtatgtagag tgcgatcgcc acatcatgga
    20521 gtctggacaa gctgaactca atattatcga aacattgcag caagcagacg gaaaacaaat
    20581 ctggtcgaat acaaataaaa ttcccctacg cgatagagaa ggaaacgttt ttggcatcct
    20641 tggcacgtct gaagacatca ccgaatacca ccaagcacaa caattgttgc aacagcaaaa
    20701 acaaaaatta gaacaagcat tgcaagaact aaaaacgatg caattgcaat tagtacaagg
    20761 tgaaaaaatg tctgcccttg gtaacttggt agcaggtgtt gctcatgaaa tcaataatcc
    20821 tgtcggcttc attgccggta acattgaacc agctaaagat tatattaaag acttgtttgc
    20881 actgattaat ctttatcagc aaaagtttcc caatcccggc tcagatattc aag
//
