LOCUS       NC_009620              22188 bp    DNA     circular CON 15-MAR-2024
DEFINITION  Sinorhizobium medicae WSM419 plasmid pSMED01, complete sequence.
ACCESSION   NC_009620
VERSION     NC_009620.1
KEYWORDS    RefSeq.
SOURCE      Sinorhizobium medicae WSM419 (Ensifer medicae WSM419)
  ORGANISM  Sinorhizobium medicae WSM419
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
REFERENCE   1  (bases 1 to 1570951)
  AUTHORS   Reeve,W., Chain,P., O'Hara,G., Ardley,J., Nandesena,K., Brau,L.,
            Tiwari,R., Malfatti,S., Kiss,H., Lapidus,A., Copeland,A., Nolan,M.,
            Land,M., Hauser,L., Chang,Y.J., Ivanova,N., Mavromatis,K.,
            Markowitz,V., Kyrpides,N., Gollagher,M., Yates,R., Dilworth,M. and
            Howieson,J.
  TITLE     Complete genome sequence of the Medicago microsymbiont Ensifer
            (Sinorhizobium) medicae strain WSM419
  JOURNAL   Stand Genomic Sci 2 (1), 77-86 (2010)
   PUBMED   21304680
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570951)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T.,
            Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M.,
            Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
            Mikhailova,N., Reeve,W. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of Sinorhizobium medicae WSM419 plasmid pSMED01
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 1570951)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Glavina del Rio,T.,
            Dalin,E., Tice,H., Pitluck,S., Chain,P., Malfatti,S., Shin,M.,
            Vergez,L., Schmutz,J., Larimer,F., Land,M., Hauser,L., Kyrpides,N.,
            Mikhailova,N., Reeve,W. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000017145.1-RS_2024_03_15
            Annotation Date                   :: 03/15/2024 03:53:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,520
            CDSs (total)                      :: 6,452
            Genes (coding)                    :: 6,144
            CDSs (with protein)               :: 6,144
            Genes (RNA)                       :: 68
            rRNAs                             :: 3, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 3, 3, 3 (5S, 16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 308
            CDSs (without protein)            :: 308
            Pseudo Genes (ambiguous residues) :: 0 of 308
            Pseudo Genes (frameshifted)       :: 194 of 308
            Pseudo Genes (incomplete)         :: 203 of 308
            Pseudo Genes (internal stop)      :: 37 of 308
            Pseudo Genes (multiple problems)  :: 111 of 308
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 22:32:10
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 356980
            Orig. end                         :: 379168
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP000739.1.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4001622
            Source DNA and bacteria available from Wayne Reeve
            (reeve@murdoch.edu.au)
            Contacts: Wayne Reeve (reeve@murdoch.edu.au)
            Paul Richardson (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LLNL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..22188
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="redox-cofactor"
                     /region_number="1"
                     /rules="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /tool="antismash"
     cand_cluster    1..22188
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(cds(PF04055 and (TIGR04085 or SPASM))
                     and (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /kind="single"
                     /product="redox-cofactor"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22188
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12188](-)"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12188)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(cds(PF04055 and (TIGR04085 or SPASM)) and
                     (TIGR02111 or TIGR03966) and minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE]))"
                     /neighbourhood="10000"
                     /product="redox-cofactor"
                     /protocluster_number="1"
     gene            242..937
                     /locus_tag="SMED_RS19570"
                     /old_locus_tag="Smed_3903"
     CDS             242..937
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098031.1"
                     /locus_tag="SMED_RS19570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3903"
                     /product="BON domain-containing protein"
                     /protein_id="WP_011969535.1"
                     /transl_table=11
                     /translation="MAGKKSWSRDVYEHYPESDRRWRARSYEDERRRDPAEGSRLVGDE
                     NSEYFPGAEPGRAGAFRGTGKTHSRWNGERDYGPDTFGGGGYHGDDDYGHRYRHGGRGI
                     EGRGFLERASDEVSSWFGDEDAERRRMQDQHRGKGPKGYTRSDTRIQEDVSDRLSDEGM
                     LDASGIAVSVANGEVQLSGLVDSKWAKRRAENCADAVSGVKHVQNNLRVRPSGGAPGWN
                     AGNTDRNIA"
     gene            complement(990..1493)
                     /locus_tag="SMED_RS19575"
                     /old_locus_tag="Smed_3904"
     CDS             complement(990..1493)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969536.1"
                     /locus_tag="SMED_RS19575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3904"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_011969536.1"
                     /transl_table=11
                     /translation="MRTVNITLAAIMLCFVSIQAVSAQTKPTEPQEFARKMSMSNLFEL
                     KSAELARQRGEGRQVLEFAERMLADHARAGKELAEAARKDGVELSQSLDKAGQERLAAL
                     QALNGATFDPAYLSSQVTAHEDALELLKTYAERGEAGALKTFAESAYPTVRTHLLQVQS
                     ITSP"
     misc_feature    1646..1659
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.53"
                     /tool="antismash"
     gene            1672..1851
                     /locus_tag="SMED_RS19580"
                     /old_locus_tag="Smed_3905"
     CDS             1672..1851
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969537.1"
                     /locus_tag="SMED_RS19580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3905"
                     /product="hypothetical protein"
                     /protein_id="WP_011969537.1"
                     /transl_table=11
                     /translation="MQTALFIAIPALPLGTALLLLWFGPLWRQQAAPWNRSSIWCHGSP
                     VLDRQGPWRTLDPT"
     gene            2057..2458
                     /locus_tag="SMED_RS19585"
                     /old_locus_tag="Smed_3906"
     CDS             2057..2458
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528667.1"
                     /locus_tag="SMED_RS19585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3906"
                     /product="cupin domain-containing protein"
                     /protein_id="WP_011969538.1"
                     /transl_table=11
                     /translation="MKTSRILAAALLAVGSGLALHAANAQQAGLHRTDLLQQDIGLPGR
                     EAIQVRVDFDGGAVSIKHSHPGEEVAYVLEGSLEYQLEGRAPVTLNAGEALFIPAGVAH
                     VARNVGSGKASELATYIVEKGSPLVVPAE"
     misc_feature    complement(2635..2648)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 18.1"
                     /tool="antismash"
     gene            2676..3413
                     /locus_tag="SMED_RS19590"
                     /old_locus_tag="Smed_3907"
     CDS             2676..3413
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528683.1"
                     /locus_tag="SMED_RS19590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3907"
                     /product="response regulator"
                     /protein_id="WP_011969539.1"
                     /transl_table=11
                     /translation="MEHIDHILIVDDDREIRELVSAYLKKNGLRATAVADGRQMRTFLE
                     ANTVDLIVLDIMMPGDDGLVLSRELRVGRHKATPIVMLTARSDEMDRIIGLEMGADDYL
                     VKPFSARELLARIKAVLRRARMLPANLQVSEAGQLLRFGQWRLDTTARHLVDEDGTAIA
                     LSGAEYRLLRVFVDHPQRVLNRDQLLNLTQGREAELFDRSIDLLVSRVRQRLGDDAREP
                     TYIKTVRSEGYVLSMPVEIVEPR"
     gene            3413..4738
                     /locus_tag="SMED_RS19595"
                     /old_locus_tag="Smed_3908"
     CDS             3413..4738
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1003: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969540.1"
                     /locus_tag="SMED_RS19595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3908"
                     /product="ATP-binding protein"
                     /protein_id="WP_011969540.1"
                     /transl_table=11
                     /translation="MVRRTGLGVNLWPHTLRARLFVILLAGLTIAHAMSFAVLFSERYI
                     AARSVMFNTLETDIATSIAILDRLPAAERASWLDRLARGSYRFVLGPGIPGDPVLTRAD
                     AEVADKVRAAIGAQYPIELESVPGGIRRLQAHLRLSDGEPLTIDVTPRGVMPVADWLPF
                     VLVAQLSLLVLCSWLAVRQATRPLADLARAADTLDPNSKTPRLSETGPREVAYAATAFN
                     AMRDRIAQYLEERVQILAAISHDLQTPITRMKLRAEMADDSIERDKLIRDLEEVERLVK
                     EGVAYARSAHGKDEKASRIDLASFVQSLAYDYQDTGKAVTVGELGDGTIVTRPHALRRI
                     LTNLVDNALKFGGSAEIELQRRADGTVLIKVSDRGPGIPEDQLDAVLQPFFRLEQSRNR
                     DTGGTGLGLAIAQQLAIVIGASLTLRNRDGGGLAAELAIGAA"
     gene            complement(4819..5478)
                     /locus_tag="SMED_RS19600"
                     /old_locus_tag="Smed_3909"
     CDS             complement(4819..5478)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969541.1"
                     /locus_tag="SMED_RS19600"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3909"
                     /product="VOC family protein"
                     /protein_id="WP_011969541.1"
                     /transl_table=11
                     /translation="MTADSTRNAPVDMKLEIVVVPVSDIDRAKEFYTSLGWRLDADFAG
                     PDDYRVIQFTPPGSGASVIFGKHVTDAEPGSAQGLYLIVSDIEAAREALRGRGVDISEV
                     FHAGDVHAGKDEPYLFGRLRVGGPDPERRSYRSYASFRDPDGNGWLLQEVTTRLPGRVD
                     ASATTFSSAADLASAFRRAEAAHGEYEKQLGHRDEDWPVWYADYIVREQSGEPRPS"
     gene            complement(5691..6737)
                     /locus_tag="SMED_RS19605"
                     /old_locus_tag="Smed_3910"
     CDS             complement(5691..6737)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (75-206). E-value:
                     1.8e-25. Score: 81.9. Matches aSDomain:
                     nrpspksdomains_SMED_RS19605_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017271003.1"
                     /locus_tag="SMED_RS19605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3910"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_011969542.1"
                     /sec_met_domain="PF00561 (E-value: 1.4e-22, bitscore: 77.0,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Abhydrolase_6 (E-value: 5.6e-13, bitscore:
                     46.5, seeds: 455, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGEFNLPRRRFMRVAALAVAAAQFGMGRAALAESARGTSIPATA
                     PGTNTSFAEIKQVNAGVLNIGYAEAGRRDAPVVILLHGWPYDIHTYVDVAPLLAKAGYR
                     VIVPYLRGYGTTRFLSADTPRNGQQAAIAADIVALMDALGLDKAVVAGCDWGARTANII
                     AALWPERCKAMVSVSGYLIGSREANRMPLPPKAELAWWYQFYFATERGRAGYEQHRNDF
                     ARLIWQLASPKWEFDDATFARSAAALENPDHVDITIHNYRWRISLADGEREFDELEDRL
                     ASLPVITVPTITLEGDANGAPHPDPAAYAKKFSGKYEHRLITGGIGHNLPQEAPQAFAQ
                     AVVDADRL"
     aSDomain        complement(6120..6512)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMED_RS19605_Abhydrolase_1.1"
                     /evalue="1.80E-25"
                     /label="SMED_RS19605_Abhydrolase_1.1"
                     /locus_tag="SMED_RS19605"
                     /protein_end="206"
                     /protein_start="75"
                     /score="81.9"
                     /tool="antismash"
                     /translation="PVVILLHGWPYDIHTYVDVAPLLAKAGYRVIVPYLRGYGTTRFLS
                     ADTPRNGQQAAIAADIVALMDALGLDKAVVAGCDWGARTANIIAALWPERCKAMVSVSG
                     YLIGSREANRMPLPPKAELAWWYQFYF"
     gene            complement(7014..7688)
                     /locus_tag="SMED_RS19610"
                     /old_locus_tag="Smed_3911"
     CDS             complement(7014..7688)
                     /GO_function="GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243467.1"
                     /locus_tag="SMED_RS19610"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3911"
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="WP_011969543.1"
                     /transl_table=11
                     /translation="MKILMVLTSHDRLGDTGKPTGFWLEEFAAPYYVFKDAGAEVTLAS
                     PKGGQPPIDPKSDEPASQTAAMERFRDDPAARDRLANTLKLGDVRQEGYDAVFYPGGHG
                     PMWDLVEDRNSIALIEGFYDAGKPVAAVCHGPAVLTRVTHHGEPIVKGRRVTGFTNSEE
                     EAVQLTDVVPFLVEDELKRLGGRYEKAEDWADFTIVDGKLVTGQNPSSSTSAAMELLKL
                     LQ"
     misc_feature    7850..7865
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.98"
                     /tool="antismash"
     gene            7885..8832
                     /locus_tag="SMED_RS19615"
                     /old_locus_tag="Smed_3912"
     CDS             7885..8832
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969544.1"
                     /locus_tag="SMED_RS19615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3912"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_011969544.1"
                     /transl_table=11
                     /translation="MDRIDAMKVFVAAIDEGSLAGAARRLKRSPTAVSRALSFLEAHVG
                     VELLHRTTRTLKLSEAGQRYAAACRRVLTDLEEADMLAGGESSAPRGILTISAPPILGE
                     EILRPMLDDFLDLYPTVSARLLLLDRFVNLVDEGVDVALRIGHLADSSLISTRLGGDVR
                     RVVVASPGYLANHPRIEEPADLAKHQILAFTNFGLESWSFTPAMGSSIPRTVQFTPRCI
                     VNSVRAAAASAAAGRGLTRLYSYHVAEYVRDGRLEIVLAGAEHPPLPAHLLAPQGRMSV
                     PKVRAFVDFAAPRLRSEFARMAAEAGKPIIPQVG"
     gene            8955..9668
                     /locus_tag="SMED_RS19620"
                     /old_locus_tag="Smed_3913"
     CDS             8955..9668
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (6-118). E-value: 2.9e-07. Score:
                     22.5. Matches aSDomain:
                     nrpspksdomains_SMED_RS19620_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269431.1"
                     /locus_tag="SMED_RS19620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3913"
                     /product="SDR family oxidoreductase"
                     /protein_id="WP_011969545.1"
                     /transl_table=11
                     /translation="MSNEQKVVIVTGASQGIGAGLVRAYRDRNYRVVATSRSIKPSGDP
                     DIHTVAGDISKPETAERIVREGIEHFGRIDSLVNNAGVFLAKPFIEMTQEDYEHNLGVN
                     VAGFFHITQRAAVEMLKQGSGHIVSITTSLVDQPMVGMPSALASLTKGGLNAVTRSLAM
                     EFSRSGVRVNAVSPGVIKTPMHPIETHSALAALHPVGRMGDISDVVGAVLYLENAGFIT
                     GEILHVDGGQNAGRW"
     aSDomain        8973..9308
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_SMED_RS19620_PKS_KR.1"
                     /evalue="2.90E-07"
                     /label="SMED_RS19620_PKS_KR.1"
                     /locus_tag="SMED_RS19620"
                     /protein_end="118"
                     /protein_start="6"
                     /score="22.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="VVIVTGASQGIGAGLVRAYRDRNYRVVATSRSIKPSGDPDIHTVA
                     GDISKPETAERIVREGIEHFGRIDSLVNNAGVFLAKPFIEMTQEDYEHNLGVNVAGFFH
                     ITQRAAVE"
     gene            9715..9954
                     /locus_tag="SMED_RS19625"
                     /old_locus_tag="Smed_3914"
     CDS             9715..9954
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527574.1"
                     /locus_tag="SMED_RS19625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3914"
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="WP_011969546.1"
                     /transl_table=11
                     /translation="MPIVTVQVTREGSAPGRNSVTAEEKAAIIKGVSEVLLDVLKKPLE
                     STYVVIEEVDLDNWGWGGLPTVQYRRQRVEAAKS"
     gene            complement(10001..11128)
                     /gene="pqqE"
                     /locus_tag="SMED_RS19630"
                     /old_locus_tag="Smed_3915"
     CDS             complement(10001..11128)
                     /GO_process="GO:0018149 - peptide cross-linking [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PF04055"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR04085"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: SPASM"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PTHR43273"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_12"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527575.1"
                     /locus_tag="SMED_RS19630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3915"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="WP_011969547.1"
                     /sec_met_domain="PTHR43273 (E-value: 4.9e-31, bitscore:
                     104.4, seeds: 35, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 8.8e-24, bitscore: 81.2,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_12 (E-value: 9.8e-07, bitscore: 25.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /sec_met_domain="SPASM (E-value: 9.2e-10, bitscore: 35.4,
                     seeds: 166, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR04085 (E-value: 2.3e-10, bitscore:
                     37.2, seeds: 281, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDTIARPADAVVAAPRVPPPMAMLAELTHRCPLACPYCSNPIAL
                     TQAKEELSTDEWVDVFAQAADLGVLHLHLSGGEPASRRDLVDLTQAAGSLGLYTNLITS
                     GVGLTEARMNSLAEAGLDHIQLSIQGVSPESADRIGGYRGGYERKMAVAGWATNAAIPL
                     TLNAVCHRQNMGEIDEIIELAFLLKARRVEIATVQFHGWAERNKELLMPTREQVERATR
                     TVAEAREAYQGILVIDYVPADYYSKYPKACMGGWGRVGLNITPSGRVLPCHAAETIPSL
                     SFENVRDSALSKIWYDSDAFNAYRGEDWMPELCRNCDRKKVDFGGCRCQAMTLAGDASA
                     TDPVCIRSPLRDRLTREVEQLAAPSTTTTNYRGRA"
     gene            complement(11125..11421)
                     /gene="pqqD"
                     /locus_tag="SMED_RS19635"
                     /old_locus_tag="Smed_3916"
     CDS             complement(11125..11421)
                     /GO_function="GO:0048038 - quinone binding [Evidence IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: PqqD_RRE"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: Thurincin_rSAM_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018098044.1"
                     /locus_tag="SMED_RS19635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3916"
                     /product="pyrroloquinoline quinone biosynthesis peptide
                     chaperone PqqD"
                     /protein_id="WP_011969548.1"
                     /sec_met_domain="PqqD_RRE (E-value: 3e-28, bitscore: 93.9,
                     seeds: 9, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 9.2e-15, bitscore: 51.2,
                     seeds: 461, tool: rule-based-clusters)"
                     /sec_met_domain="Thurincin_rSAM_RRE (E-value: 2.5e-07,
                     bitscore: 27.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTADQPMISGSSVVKLARGVRLHEDTVRGQTVLLAPERAMAVDDI
                     AVAIVQALDGERTLDDIAAGFAEKFDAPVAEIAGDVTAFVQELSVRRMLEIVQ"
     aSDomain        complement(11137..11400)
                     /aSDomain="PqqD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing protein in a pyrroloquinoline
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_SMED_RS19635_PqqD_RRE.1"
                     /evalue="5.70E-30"
                     /identifier="RREFam006.1"
                     /label="SMED_RS19635"
                     /locus_tag="SMED_RS19635"
                     /protein_end="95"
                     /protein_start="7"
                     /score="93.9"
                     /tool="antismash"
                     /translation="ISGSSVVKLARGVRLHEDTVRGQTVLLAPERAMAVDDIAVAIVQA
                     LDGERTLDDIAAGFAEKFDAPVAEIAGDVTAFVQELSVRRMLE"
     aSDomain        complement(11155..11319)
                     /aSDomain="Thurincin_rSAM_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing rSAM protein in a thurincin
                     sactipeptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_SMED_RS19635_Thurincin_rSAM_RRE.1"
                     /evalue="4.90E-09"
                     /identifier="RREFam019.1"
                     /label="SMED_RS19635"
                     /locus_tag="SMED_RS19635"
                     /protein_end="89"
                     /protein_start="34"
                     /score="27.1"
                     /tool="antismash"
                     /translation="APERAMAVDDIAVAIVQALDGERTLDDIAAGFAEKFDAPVAEIAG
                     DVTAFVQELS"
     gene            complement(11418..12188)
                     /gene="pqqC"
                     /locus_tag="SMED_RS19640"
                     /old_locus_tag="Smed_3917"
     CDS             complement(11418..12188)
                     /EC_number="1.3.3.11"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     redox-cofactor: TIGR02111"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969549.1"
                     /locus_tag="SMED_RS19640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3917"
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="WP_011969549.1"
                     /sec_met_domain="TIGR02111 (E-value: 1e-126, bitscore:
                     417.6, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTADDRQAFHARLLEIGKQRYHDKHPFHAMLHSGGCTTTQVRAW
                     VINRYYYQSRIPMKDAAFMSRCDDPDLRRAWRSRIEDHDGDVEEGGGIRRWLRLAEAVG
                     LDPTYVASTKGVLPSTRFAVDAYVSFVREKPLLEAVASSLTELFAPRIHSERIAGLLEH
                     YAFADESALAYFRQRLAEAPRDVEFGLAFVLDRADTREKQDAAAAALAFKTDVLWSQLD
                     ALYSAYVSPGRIPPGGWDGTEGVVREPRAREAAE"
     gene            complement(12185..13096)
                     /gene="pqqB"
                     /locus_tag="SMED_RS19645"
                     /old_locus_tag="Smed_3918"
     CDS             complement(12185..13096)
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017271801.1"
                     /locus_tag="SMED_RS19645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3918"
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="WP_011969550.1"
                     /transl_table=11
                     /translation="MNKTPDLRILVLGAAAGGGLPQWNCGCPNCAMARDPGSGLKPQTQ
                     SSLAVSLDGEAWAVFNASPDIRQQIHANRPLQPRRLRHSPIESVVLTNGDIDHLAGLLV
                     LREKQAFALFSTGAVGRIVADNPVFQVLDPELVSKRTIEIEETFSPLQGLGARLFAVPG
                     KVPLFLENGEPDLGLEGEHTVGVELAAAGKRVYYVPGCAVMTDALAARLRNADAVFFDG
                     TLYSDDEMIQTGMGRKTGRRMGHMPIDGEGGSLDALGTLNIRRKIYVHINNTNPIWRAG
                     SERERVEAQGFEIGYDGMEVRL"
     gene            complement(13138..13233)
                     /gene="pqqA"
                     /locus_tag="SMED_RS19650"
     CDS             complement(13138..13233)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /gene="pqqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528714.1"
                     /locus_tag="SMED_RS19650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pyrroloquinoline quinone precursor peptide PqqA"
                     /protein_id="WP_003528714.1"
                     /transl_table=11
                     /translation="MAWHKPKFIEVSCAMEITRYAPADGDEPILF"
     gene            complement(13605..14546)
                     /locus_tag="SMED_RS19655"
                     /old_locus_tag="Smed_3919"
     CDS             complement(13605..14546)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527576.1"
                     /locus_tag="SMED_RS19655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3919"
                     /product="LysR family transcriptional regulator"
                     /protein_id="WP_011969551.1"
                     /transl_table=11
                     /translation="MRNVSLRQLRTVEAVCRLGKINLAAEALGLTGPALTLQIQHLERD
                     TGVALFDRTRGGMVPTAYGLAFLEAARAVEDSLAALEDSIGAISGLRTGRLRLGVVSTG
                     KYFAPQLIAAFRDQVPAVEINLFIGNRAEIIAKLRDHEIDIALMGRPPRDFEVRAQVFG
                     DHPLVFIAPAGHPLAGVLEISRERIAREQFLVREKGSGTRISLEIFLSDTPHELEELGT
                     EIASNETIKQAVIAGLGIAFISAHTIEQEVKLGRLVILDVIDTPIRRQWFTVSRLDRAA
                     TPAMQAFEQFVLSSGARYLPVVSKPYPANAFG"
     misc_feature    complement(14584..14600)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 19.38"
                     /tool="antismash"
     gene            14648..15697
                     /locus_tag="SMED_RS19660"
                     /old_locus_tag="Smed_3920"
     CDS             14648..15697
                     /EC_number="3.1.3.11"
                     /GO_function="GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763849.1"
                     /locus_tag="SMED_RS19660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3920"
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="WP_011969552.1"
                     /transl_table=11
                     /translation="MSGATLEAYLASCTTHGDELSRDVAAVIQRLAKAALDIRKLVNQG
                     ALGTVFNGMHSGSNTDGDVQKDLDILCDDQFLSCLQGAPVACYASEELENPVLLDPAAR
                     LAVAIDPLDGSSNIDNNISIGTIFSVLPAAKGPDVDPSQSFLQPGNRQLAAGFFVYGPQ
                     TALVLSLGRGTEIFIFSSRLGCFVDAYKSVGIPDRANEFAINMSNYRHWEEAIRLYVDD
                     CLAGSEGPRERDFNMRWIASLVAEAYRILVRGGIFLYPADSRKGYSHGRIRLVYEANPI
                     AFIVENAGGSATTSVDRILDLVPESLHQRVPLVFGSRREVARITRYHVDPNMIGERAPL
                     FGKRGLFRA"
     gene            15708..16577
                     /locus_tag="SMED_RS19665"
                     /old_locus_tag="Smed_3921"
     CDS             15708..16577
                     /EC_number="2.7.1.19"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014763848.1"
                     /locus_tag="SMED_RS19665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3921"
                     /product="phosphoribulokinase"
                     /protein_id="WP_011969553.1"
                     /transl_table=11
                     /translation="MSAKYPIISITGSSGAGTTTVKDTFEKIFKRENISASFIEGDAFH
                     RYDRETMRSKIAEEKARGVDFTHFSAEANELEILESVFAEYGRRGVGRTRHYVHDDAEA
                     VKFGSDPGTFTDWEEFRDSDLLFYEGLHGCAVTDTINLAQHCDLKIGVVPVINLEWIQK
                     IHRDKATRGYSTEAVTDTILRRMPDYVHYICPQFSLTDINFQRVPIVDTSNPFIARWIP
                     TPAESILVIRFAKPQSIDFPYLLSMLHNSYMSRANSIVVPGDKLDLAMQLIFTPLIHKL
                     LERKHRMS"
     gene            16588..18675
                     /gene="tkt"
                     /locus_tag="SMED_RS19670"
                     /old_locus_tag="Smed_3922"
     CDS             16588..18675
                     /EC_number="2.2.1.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="tkt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DXP_synthase_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1204: transketolase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975102.1"
                     /locus_tag="SMED_RS19670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3922"
                     /product="transketolase"
                     /protein_id="WP_011969554.1"
                     /sec_met_domain="DXP_synthase_N (E-value: 4.7e-08,
                     bitscore: 29.0, seeds: 174, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNVSQQIEPRAAASERNMADAIRFLSMDAVEKANSGHPGMPMGMA
                     DAVTVLFNRFIRIDPSLPDWPDRDRFVLSAGHGSMLLYSLHHLIGFADMPMAELSSFRQ
                     LGSKTAGHPEYGHALGIETTTGPLGQGISTAVGMAMAEQMMASRFGSALCNHFTYVVAG
                     DGCLQEGISHEAIDLAGHLKLRKLVVLWDDNRISIDGSTDLSTSMNQLARFRAASWDAQ
                     AVDGHDPEAVAKALERARRTRKPSLIACRTRIGKGAASMEGSHKTHGAALGDKEIAATR
                     EKLGWPHPPFFVPPEIRAAWAKVAARGRTAREAWDIRLDASRSKKRYEQTIRRQFDGEL
                     GDLLAKFRSAHRTRATKVATRQASQMALEVINGATALTIGGSADLTGSNLTMTSQTQPI
                     SPGNFKGRYLHYGIREHGMAAAMNGIALHGGFIPYGGTFLVFSDYARGAMRLSALMGLP
                     VIYVLTHDSIGLGEDGPTHQPVEHLAMLRATPNLNVFRPADIIETAECWEIALGEKNTP
                     SVLALSRQALPMLRRTEGNENQSALGAYVLREARGNRDITILATGSEVEIAVAAAERLQ
                     AEEGIAAAVVSMPCWEKFEVQDLAYRRKVLGDAPRIAIEAAGRLGWDRWMGPDGAFVGM
                     TGFGASAPAGDLYRHFGITADHVVAEALELLRRAYSETLPIGARIGPHPSAHTVRSSQE
                     A"
     gene            18678..19757
                     /gene="fba"
                     /locus_tag="SMED_RS19675"
                     /old_locus_tag="Smed_3923"
     CDS             18678..19757
                     /EC_number="4.1.2.13"
                     /GO_function="GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="fba"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975101.1"
                     /locus_tag="SMED_RS19675"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate in
                     the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate in
                     the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /old_locus_tag="Smed_3923"
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="WP_011969555.1"
                     /transl_table=11
                     /translation="MARITLRQLLDHAAERSYGVPAFNINNMEQGLAIMEAARACDAPV
                     ILQVSRGARSYANDIMLAKMMEALEVMYPDIPLCIHQDHGNNVATCLTAIQHGFTSVMM
                     DGSLKEDAKTPADYAYNAMITTEVSRLAHMVGASVEGELGCLGSLETGHGEAEDGHGFE
                     GALDRSQLLTDPDEAARFVAETGVDALAVAIGTSHGAYKFTRKPTGEVLAMDVIEKIHE
                     RLPDTHIVMHGSSSVPQEWQDVFNAHGGEMRETYGVPVEEIVRGIRFGVRKVNIDTDLR
                     LAAAAAFRRVADTSRTEFDPRKFLKPAMDAMSAVCKARFEAFGTAGNASRIKVVPMAEM
                     ARRYASGSLKPQAKRAEAA"
     gene            19788..21248
                     /locus_tag="SMED_RS19680"
                     /old_locus_tag="Smed_3924"
     CDS             19788..21248
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969556.1"
                     /locus_tag="SMED_RS19680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3924"
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="WP_011969556.1"
                     /transl_table=11
                     /translation="MNADAKTEIKGRERYKAGVLKYAQMGYWNGDYEPKDTDLIALFRI
                     TPQDGVDPIEAAAAVAGESSTATWTVVWTDRLTACDQYRAKAYRVDPVPGTPGQYFCYV
                     AYDLILFEEGSIANLTASIIGNVFSFKPLKAARLEDMRLPVAYVKTFKGPPTGIVVERE
                     RLDKFGKPLLGATTKPKLGLSGKNYGRVVYEGLKGGLDFMKDDENINSQPFMHWRDRYL
                     YCMEAVNHASAVTGEVKGHYLNVTAGTMEEMYRRAEFAKELGSVIVMVDLIIGWTAIQS
                     MSEWCRQNDMILHMHRAGHGTYTRQKNHGISFRVIAKWLRLAGVDHLHAGTAVGKLEGD
                     PLTVQGYYNVCREMKNAVDLPRGLFFEQDWADLKKVLPVASGGIHAGQMHQLLDLFGDD
                     VVLQFGGGTIGHPMGIQAGATANRVALEAMVLARNEGRDIAHEGPEILRAAAKWCKPLE
                     AALDTWGNISFNYTPTDTSDFVPSVTAA"
     gene            21272..21661
                     /locus_tag="SMED_RS19685"
                     /old_locus_tag="Smed_3925"
     CDS             21272..21661
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014527580.1"
                     /locus_tag="SMED_RS19685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Smed_3925"
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="WP_011969557.1"
                     /transl_table=11
                     /translation="MRITQGCFSFLPDLTDEQITAQVEYCLGRGWAIGVEYTDDPHPRN
                     TYWEMWGNPMFDLRDAKGVMMEVEDCRKAHPQDYIRLNAFDSSRGLETVTMSFIVNRPE
                     NEPSLRMTRTESDGRSQHYTWETQR"
     misc_feature    22013..22027
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.19"
                     /tool="antismash"
ORIGIN
        1 aaatggcata ggcgacggca gcaccgagga tgaaggcacc gccgcctgtg acaaaaagcc
       61 agaaatagtt cgtcagcatc gtcgtatctc cttgccagag ccaaccggct cggccgccga
      121 aggttccaga cgagcccgtt tctcaccttt ccttcgtctt gggcgaagct tgcattgcac
      181 cggaaccgag cgtctgctcg ccggtttgac cgatgtcagc caccgagcaa aggagaacct
      241 catggccgga aaaaagagct ggagccgcga cgtctatgaa cactatccgg aatcggatcg
      301 gcgctggcgt gcccgctcgt acgaggacga gaggcgtcgc gatcctgccg agggttcgcg
      361 gctcgtgggg gacgagaact cagagtattt cccaggtgcc gaacccggtc gcgccggcgc
      421 gtttcgcggc acaggaaaaa cccatagccg ttggaacgga gaacgcgact atggcccgga
      481 taccttcggc ggcgggggct accacggcga cgacgattac ggccaccgat atcgccatgg
      541 cggcaggggc atcgaggggc gcggctttct cgaacgcgcc agtgacgaag tctcgtcctg
      601 gtttggcgac gaggacgccg aacgcagacg catgcaggac cagcatcgtg gcaagggccc
      661 caaaggctac acccgttccg acacccgcat tcaggaagat gtcagtgatc ggttgagcga
      721 cgaggggatg ctcgatgctt ccggcatagc cgtctcggtc gcaaatggag aagttcaatt
      781 gagcgggttg gtcgattcga agtgggccaa acgccgcgcc gagaactgcg ccgacgccgt
      841 ctccggcgtg aaacatgttc agaacaatct ccgcgtccga ccgtcgggag gcgcccccgg
      901 ctggaatgcc gggaacaccg accgcaacat tgcttgaacg cacaagatcg tgggccgtcg
      961 ccttggtcac atccctgcaa gcttcccggt tacggagatg taatcgactg aacctggagc
     1021 aaatgcgttc tgacggtcgg atatgcggat tcggcaaacg ttttcagggc cccggcctcg
     1081 cctcgctcag cataggtctt gagaagttcc agcgcatcct catgcgcagt aacctgcgac
     1141 gacagataag ccggatcgaa ggtcgcgccg tttagcgcct gcaaggcagc aagcctctcc
     1201 tgcccggcct tgtcgagcga ctgggaaagc tccacgccgt ccttccgtgc tgcttcggcc
     1261 agttccttgc cggcacgcgc atgatcagcg agcatccgct cggcgaactc cagcacctgg
     1321 cgtccctcgc cccgctgccg cgccagttcc gccgatttca gctcgaaaag gttcgacatc
     1381 gacattttcc gcgcgaactc ctgaggctcc gttggctttg tctgagcgct gaccgcctga
     1441 atcgagacga agcacagcat aattgctgca agggtgatgt tgactgttct cattggggca
     1501 cctccggaac cgctctaacc tccgggctcg ctcatcgttc cgcagttccg gtggagcacg
     1561 gacggccgca ggccggtcta aggaactttc ttggggccgg tctggttgta tcaaccgaga
     1621 ggccgcgcaa cggcctatgg gagcggtcac cccgcgtgac cgactaagct tatgcaaacg
     1681 gcactgttca tcgcgatccc ggccctgccg ctcggaacgg cgctcctgct tctctggttc
     1741 ggtccgctct ggcgccagca agcggcccca tggaaccgct cgtcgatctg gtgccacgga
     1801 tcacccgttc tggaccggca aggcccttgg cgaacactcg atcctacctg agcacgcgtc
     1861 gggacgaaaa gggcgagcgc ttcatggtgc attcggcttg atggacgccc cttgaccagc
     1921 tcgcgccaac gctgccctga cacaggccgt tacaaaaatc ccatccagcg cacacatcgc
     1981 caatacatgc cggcggttca atggtcgcac tggcaacgag gacgagcctc agccgatcgt
     2041 ggaaagggat acctcaatga aaacgtctcg gatcttggca gccgcgctgc tggccgttgg
     2101 aagcggatta gcgctgcatg cggccaatgc gcaacaggcg ggattgcatc gcaccgatct
     2161 cctgcagcag gatatcggcc tgcccgggcg agaggcaatt caggtgcgcg tcgacttcga
     2221 cggcggtgcg gtttccatca agcactccca tccgggcgaa gaggtggcct atgtgctgga
     2281 aggctcgctg gaatatcagc tcgaggggag agccccggtc acgctaaacg ccggcgaggc
     2341 cctgttcatt ccggccggtg tggctcatgt cgccagaaat gtcggtagcg gtaaggcgtc
     2401 ggaactggcg acctatatcg tggaaaaggg atctccgctc gtggttccag ctgagtgagc
     2461 gtcagagatt taccccttgg ctttagtttc gacatcgcgc tctgttgaat tgcgaacatg
     2521 gtccggcggc ggtgccgcat ggtagcgccg ggcctgttga ccgccgactt gcggaccgcg
     2581 ttccggttct tgctcgtgca cgatccggcg agtacagcta ccaaggcatt ttcgtttcga
     2641 tttaaaatgg cagtcacagg ggttggagat cgcgcgtgga gcatatcgat cacatcttga
     2701 tcgttgatga cgatcgggag atccgggaac ttgtatcggc ttacctgaag aagaacggcc
     2761 tgcgggcgac cgccgtggcc gacgggcgtc agatgcggac gttcctcgag gcaaatacgg
     2821 tcgatctgat cgtgctcgac atcatgatgc cgggcgatga cggcctcgtg ttgagccgcg
     2881 aactgcgtgt cggccggcac aaggcgacgc cgatcgtcat gctgaccgcc cgctccgacg
     2941 agatggatcg catcatcggg ctggaaatgg gcgccgacga ctatctcgtc aaaccattct
     3001 cggcgcgcga actgctggcc cgcataaagg cagtcctgcg gcgcgcgcgc atgctgcctg
     3061 caaatctgca ggtctcggag gcaggtcagc tgttgcgttt cggccaatgg aggcttgata
     3121 cgacggcgcg gcatcttgtc gacgaagacg gtaccgcaat agcgctcagc ggcgcggaat
     3181 accggctgct gcgcgtcttc gtcgatcacc cgcagcgcgt cctcaaccgc gatcagctcc
     3241 tcaacctcac gcaggggcgc gaggccgaac tcttcgaccg ctccatcgat ctcctggtga
     3301 gccgggtgcg tcagcggctc ggcgacgatg cccgcgagcc gacctatatc aagacggtcc
     3361 ggagcgaggg ttacgttctt tctatgccgg tcgagatcgt ggaacctcgc tgatggtgcg
     3421 ccgaacgggc cttggagtta acctgtggcc gcacacgctc agggcgcggc ttttcgtcat
     3481 cctcctcgcc gggctgacga ttgcacatgc gatgtcgttt gctgtgctgt tttcggaacg
     3541 ctacattgcc gccagatcgg tgatgttcaa cacgcttgag accgacattg cgacgtcgat
     3601 cgccatcctc gaccggctcc ccgcggccga acgcgccagc tggctggacc ggctcgctcg
     3661 cggatcatac cggttcgttc tggggccggg cattcccggt gatccggtgc tcacccgggc
     3721 cgacgccgaa gtcgccgaca aggttcgggc tgcgataggc gcgcaatacc ccattgagct
     3781 tgaatcggtt cccggcggca ttcgccgcct tcaggcgcat cttcgcctga gcgacggcga
     3841 gcccctgacg atcgacgtga cgccaagagg ggtcatgccg gttgccgact ggttgccttt
     3901 cgttctcgtc gctcagcttt ccctcctggt cctgtgcagc tggcttgctg tgcgccaggc
     3961 gacccgtcct cttgccgacc tcgctagggc cgccgacacg ctcgacccga acagcaagac
     4021 gccgcgtctc agcgagaccg gcccgaggga agtcgcctat gcggcgacgg cgttcaatgc
     4081 gatgcgggat cggatagcgc agtatctgga ggagcgggtc cagatactgg ccgcgatttc
     4141 gcacgatctg cagacaccga tcacgcgcat gaaactgcgc gcggaaatgg cggacgattc
     4201 catcgaaagg gacaagctga tcagggatct cgaagaggtt gaacgtctcg tgaaggaagg
     4261 tgtcgcctat gcgcgcagcg cccacggcaa ggacgaaaaa gcatcgcgca tcgatctcgc
     4321 ctccttcgtc cagagtcttg cctatgatta tcaggataca ggcaaagcgg tcaccgtggg
     4381 ggaattggga gatggcacaa tcgtgacgag gccgcacgcc ttgagacgca ttctcaccaa
     4441 cctggtcgac aatgcgctga agttcggcgg cagtgccgag attgaacttc agcgccgcgc
     4501 cgacggcact gtgctcataa aggtgtcaga tcgaggtccc ggcattccgg aagaccagct
     4561 ggatgcggta ttgcagccct tctttcgctt ggagcaatca cgcaaccgcg atacgggcgg
     4621 gaccgggctc ggtcttgcca ttgcccaaca gctggcgatc gtcatcggtg cttccttgac
     4681 cttgcgcaat cgcgacggcg gcggccttgc ggcggagctc gccatcggag ctgcctgagg
     4741 aatggtcgac ggcgccatac agagaagcgg ggcggcgcct gccgccccgc tttgttgcgt
     4801 cgatccgggc tgcatggctc aggatggccg gggctccccg gattgctccc ggacaatgta
     4861 atctgcgtac cagacgggcc agtcctcgtc cctgtgtccg agttgctttt catactcgcc
     4921 gtgagccgcc tccgcacggc ggaatgcgct tgccagatca gcggcggagc tgaacgtcgt
     4981 ggcactggcg tcgacgcggc ctggtagtcg cgtggtgacc tcctgaagca gccagccgtt
     5041 accgtcagga tcgcggaagg aagcgtagga acgatagctg cggcgttctg ggtcagggcc
     5101 gccaaccctg agccgtccga acaggtaagg ctcgtccttg ccggcatgca catcgccggc
     5161 gtggaacact tcgctgatat cgacaccgcg accccgcaac gcctcgcggg cggcctcgat
     5221 gtcggaaacg atcaggtaca gtccctgtgc ggaacccggt tccgcatcgg tcacatgctt
     5281 cccgaagatg accgaggctc cggatccagg cggagtgaac tggatcacgc ggtagtcatc
     5341 gggtccggcg aagtcggcgt cgagccgcca gccgaggctc gtatagaatt cctttgcgcg
     5401 gtcgatatcc gacacgggca cgacgacaat ttcgagcttc atatcgacgg gcgcgtttct
     5461 ggtgctgtca gcggtcattt ttctctcctt gtatcaggag gcgcagggcg ccttttgtct
     5521 ctcgattgac gatgaaggac gggcacgttt cccgtttcgt ccgactgtcg agagacatat
     5581 gccgtcccgc acatgtatgg gatatttcgc aacagggcga aattgtaagc caatgctcac
     5641 agaccacggg acgaatacgg tctgatacaa aggcattgct ccgcgggcgg tcagagtcga
     5701 tcggcgtcca cgaccgcctg ggcgaaagcc tgtggcgctt cctgtggcag gttgtgaccg
     5761 atgccgccgg tgatcaaacg atgctcgtac ttgccggaga actttttggc ataggccgcc
     5821 ggatccggat gcggcgctcc attggcatcg ccctcgagcg tgatcgtcgg cacggttatc
     5881 accggcaagc tggcgagccg atcctccagc tcgtcaaatt cgcgctcgcc atcggcaaga
     5941 ctgatccgcc agcggtaatt gtggatcgtg atatcgacat ggtccgggtt ctcaagggcc
     6001 gccgcgctgc gcgcgaaggt cgcgtcgtcg aactcccact ttggggaagc aagctgccag
     6061 atgagccgcg cgaagtcatt ccggtgctgt tcgtacccgg cccggccgcg ttccgtggcg
     6121 aagtagaact gataccacca ggcgagctct gctttgggcg gcagcggcat cctgttggcc
     6181 tcgcggctgc cgatcagata accgctcacg gaaaccatcg ccttgcagcg ctccggccag
     6241 agtgcggcga tgatgttcgc ggttcgcgca ccccaatcgc agccggcgac gacggccttg
     6301 tcgagaccaa gcgcgtccat gagggccacg atatccgcgg ctatcgccgc ctgctggccg
     6361 ttgcggggcg tgtctgccga aagaaagcgc gtcgtgccgt agccgcgcag atagggaaca
     6421 atgacccgat agcccgcctt tgcaagcaga ggcgccacat cgacataggt gtgaatgtca
     6481 tagggccatc cgtggagcag gatgacgacg ggtgcgtcgc gcctgcccgc ctcggcatat
     6541 ccaatgttga ggacacccgc attgacctgt ttgatctccg cgaaggatgt attcgtcccc
     6601 ggcgctgtcg ccggaatgga cgtccccctc gccgattctg cgagggcagc gcgccccatg
     6661 ccgaactgtg ctgcagcgac tgccaatgcc gccactcgca tgaaccgccg ccgcggcaga
     6721 ttgaattcgc ctgtcatcga tcttctccgg attgtgtgaa ccacagtcgc ggacatgcga
     6781 tgctcatgta tcccgattgt gaagccgtcg aaccgcaatt gaatgcgcgt gtaaggttca
     6841 cagcggcaga tacaataggt tacgtttcgc ttgcctggcc gcacggcacg ggcgcactcc
     6901 cgggagcata cccggcggat ggttcgaggt gtttccagag cgcatgcgac cgggatcggc
     6961 aaaccggttt tctcaggtca cttatgtcgc agggctcaag gccgcggcaa ggcctactgc
     7021 aggagcttca gcaactccat ggcagccgac gtagacgaag acgggttctg gccggtgacc
     7081 agcttgccgt ccacgatcgt gaagtccgcc caatcctccg ccttctcgta gcgcccaccg
     7141 aggcgcttca attcgtcctc gacgaggaac ggcacgacat ccgtcagttg aacggcctcc
     7201 tcctcggaat tggtgaagcc ggtgacgcgc cttcccttga cgatgggctc gccgtgatgc
     7261 gtgacccgcg tcaggacggc cgggccatgg cagacggccg cgaccggttt gcccgcatcg
     7321 tagaagcctt cgatgagggc gatcgaattc ctgtcttcaa cgaggtccca cataggcccg
     7381 tgtccgcccg gatagaacac cgcgtcgtag ccctcctgtc tgacgtcacc gagcttcagt
     7441 gtgtttgcca atctgtcccg cgcggcgggg tcgtccctga agcgctccat cgccgcagtc
     7501 tggctcgctg gctcgtcgct tttcggatcg atcggcggct gcccgccctt cggtgatgcc
     7561 agcgtgacct ctgcccccgc gtccttgaac acgtaatagg gtgcagcgaa ttcctccagc
     7621 cagaatccgg tcggcttgcc tgtatcgccg agccggtcat gcgaagtcag aaccatcagg
     7681 attttcatgg tcattctcct ccgttgcggc gtttccagat gcaggatcgc cggggttgcc
     7741 agcctattct ccggccgtgg ccggatattg gcgaggctcc cgtgggatta ccttcgcttc
     7801 ggccatgcgc cattattgcg agctgccgtc ttcgggtgaa tactcgttca aaggcaatca
     7861 ctatcccgag gagcggcaga ataaatggac cgcattgatg caatgaaagt cttcgtcgct
     7921 gcgatcgacg agggtagcct cgccggcgcg gcccgccgcc tcaaacgatc gccgacggcg
     7981 gtgagccgtg ccctgagctt cctggaggcg catgtagggg ttgaactcct gcaccgtacg
     8041 acccgcaccc tcaagctgag cgaagcaggc cagcgctatg cggccgcctg ccgccgggtg
     8101 ctgactgacc tggaagaagc ggacatgctt gcagggggcg agagttccgc cccccgcgga
     8161 attttgacga tctctgcacc accgatcctc ggagaggaaa tactcaggcc gatgcttgac
     8221 gacttcctcg atctctaccc gaccgtctct gcccggcttc ttctgctcga ccgattcgta
     8281 aacctggtgg acgaaggtgt ggatgttgca ctccgcatcg gccatctggc ggattcgtcg
     8341 ctcatttcca cccggctcgg gggagatgtc aggcgcgtcg ttgtcgcctc gcctggctat
     8401 cttgccaatc acccccgcat cgaagaaccg gcggacctgg ccaaacatca gatcctggca
     8461 tttaccaatt tcggtcttga gtcctggagc tttacaccgg caatgggctc gtccattccg
     8521 aggacggtac agttcacgcc gagatgcatc gtcaacagcg tgcgcgccgc cgccgcttcg
     8581 gcagctgccg gacgcggtct gacccggctt tattcctatc atgtcgcgga atatgtgagg
     8641 gacgggcgcc tcgagatcgt gcttgccggc gcggaacacc cgcccctgcc ggcacatctt
     8701 ctggcaccgc agggccgaat gtcagtgccg aaggtccgcg cctttgtcga cttcgccgcg
     8761 ccgcgcctac gctctgaatt cgcgcgcatg gcagcggagg cgggcaagcc gattataccg
     8821 caggtcggat gagtgtccgt caatctgcgg caattctccc acagtctgca ctgatctaat
     8881 ttcgaatgca ccgacgccga gggcggaggg cggacgcaga aagcggccgc tgcgattgag
     8941 agaggatcat tccaatgagt aacgaacaga aagtcgtcat cgtcaccggc gcctcgcaag
     9001 ggatcggcgc cggattggtc cgcgcctatc gagaccggaa ctaccgcgtc gtcgcgacct
     9061 cgagatccat caagccgagc ggcgacccgg atatccatac ggtcgctggc gacatcagca
     9121 agccggaaac cgccgagcgg atcgtgcgcg aggggatcga gcacttcggc cgcatcgact
     9181 ctctggtgaa caatgccggc gttttcctcg ccaagccgtt catcgagatg actcaggagg
     9241 actacgagca taatctcggg gtgaacgtgg ccgggttctt ccacatcacc cagcgcgcag
     9301 ccgtggaaat gctgaagcag ggctcaggtc atatcgtcag catcaccacc agtctggtcg
     9361 accagccgat ggtcggtatg ccctccgcac ttgcctctct gacgaagggt ggcctgaacg
     9421 cggtaacccg ttcgctggcg atggaatttt ccaggagtgg tgttcgcgtc aatgccgttt
     9481 ctccgggtgt aatcaagacg cccatgcatc cgatcgagac gcattcggct ttggccgcac
     9541 tgcacccggt tggcaggatg ggcgatatca gcgacgtcgt gggtgccgtc ctctatctcg
     9601 aaaatgcagg attcatcacc ggcgagatcc tccacgtcga cggcggccag aacgccggac
     9661 gctggtaacc ggcatatccg caacacacga acgagactcg caaaggaaac tgtaatgcct
     9721 atcgtcaccg tacaagttac ccgtgaagga agcgcgcccg gccgcaattc ggtcaccgcc
     9781 gaggaaaagg cagccatcat caagggcgtc agcgaagtgc ttctggatgt cctgaaaaag
     9841 ccgttggaat ccacctatgt ggtgatcgaa gaggtcgatc tcgacaactg gggctgggga
     9901 ggccttccga cagtgcaata ccgccggcaa cgtgtcgagg cggctaaatc ctgaagcctg
     9961 atcgcttcac cgcccggtgg agcgatccag ctgttttcgg ctacgcccga ccacgataat
    10021 ttgtggtggt ggtcgatgga gccgcgagct gctcgacttc ccgcgtcagg cgatcacgaa
    10081 gcggcgatcg gatgcaaacc gggtctgtcg cactggcatc gccggcaagc gtcatcgcct
    10141 gacagcgaca gccgccgaaa tccaccttct tgcggtcgca gttccgacag agctccggca
    10201 tccagtcctc gccccgatag gcgttgaatg cgtcgctatc gtaccagatc ttcgaaaggg
    10261 cgctgtcgcg cacgttttcg aaggagaggc tcggaatggt ttccgcggca tggcatggca
    10321 gcacccgccc ggatggcgtg atgttcaggc ccacccttcc ccatccgccc atgcaggctt
    10381 tcggatactt ggaataataa tcggccggca catagtcgat caccagaatg ccctgatagg
    10441 cttcgcgcgc ttcggcgacg gttcgggtgg cgcgttcgac ctgctcacgc gtcggcatga
    10501 ggagttcctt gttccgctcg gcccagccgt ggaactgtac ggtcgcgatc tcgacccgcc
    10561 gcgccttcag cagaaacgca agctcgatga tttcgtcgat ttcgcccatg ttctgcctgt
    10621 gacagacggc gttcaacgtc agcggaatag cagcattcgt tgcccagccg gcaacagcca
    10681 tcttccgttc ataaccgccc cggtacccac cgatccgatc ggcgctttcg ggcgagaccc
    10741 cctgaatgga aagctggatg tggtcgagcc ccgcctcggc gaggctgttc atccttgcct
    10801 ctgtcaatcc cacgccagag gtgatcagat tggtgtaaag accaagcgac cccgccgcct
    10861 gcgtcaagtc cacgagatct ctgcgcgatg ccggctcccc gccggacaaa tgcagatgca
    10921 ggaccccgag atcggcggct tgcgcgaaga cgtctaccca ttcgtcggtc gacaattctt
    10981 ccttggcttg cgtcagtgcg atgggattgg agcaataggg gcaggcgagc ggacaacgat
    11041 gggtcagttc cgccagcatg gccatcggcg gaggcacgcg tggtgccgcg acaacggcgt
    11101 cggctgggcg ggcgattgtg tcgctcattg gacgatctcc agcatgcggc ggacggaaag
    11161 ctcctgcacg aacgccgtga cgtctccggc gatctctgcg accggggcgt cgaacttctc
    11221 ggcaaagccg gcggcgatat catcaagggt cctctcgccg tcaagcgcct ggacgattgc
    11281 gacggctata tcgtccaccg ccattgcccg ttccggggca aggagcaccg tctggccgcg
    11341 aaccgtatcc tcgtgcagcc taacccctcg cgccagcttg acgacgctcg atcctgaaat
    11401 cataggctga tcggccgtca ttccgctgcc tcccgcgctc tcggctcccg cacgacccct
    11461 tccgtgccat cccagcctcc cggtggaatg cgccccggcg agacgtaagc cgaataaagc
    11521 gcgtcgagct gggaccaaag aacatccgtc ttgaacgcaa gcgccgcagc ggcggcatcc
    11581 tgtttttcgc gcgtgtcggc acggtcgagg acaaaagcca gtccgaattc gacgtcccgg
    11641 ggcgcctcag ccagtctctg gcggaaatag gccagtgccg actcgtcggc gaaggcgtaa
    11701 tgctccagca gaccggcaat gcgctccgag tggatcctgg gcgcgaaaag ctccgtcagg
    11761 gaggatgcaa ccgcctcgag aagcggcttc tcacgcacga aggacacata ggcatccacg
    11821 gcaaaccggg tcgacggcag gacgcctttg gtcgatgcga cgtaagtcgg atctagcccg
    11881 accgcctcgg caagacgcag ccagcgacgg atgccgccgc cctcttcgac gtcgccgtcg
    11941 tgatcttcga tgcgggagcg ccaggcgcgg cgcagatcgg gatcatcgca acgcgacatg
    12001 aaggccgcat ctttcatggg aatgcggctt tgataatagt aacggttgat cacccaggcg
    12061 cggacctgtg tcgtcgtgca gccgccgcta tgaagcatgg catgaaacgg atgcttgtcg
    12121 tggtagcgct gcttgccgat ctccagcaga cgggcatgga aagcttgtct gtcatctgcc
    12181 gtcgtcacag ccggacctcc attccgtcgt agccgatctc gaagccctgt gcctcgacgc
    12241 gctcgcgctc gctgccggcc cgccagatcg ggttggtgtt gttgatatgg acatagatct
    12301 ttctgcggat gttcaaggta ccgagtgcat cgaggctgcc gccttcgccg tcgatgggca
    12361 tgtggcccat gcgacggccg gtcttgcggc ccataccggt ttgaatcatc tcgtcgtcac
    12421 tgtagagcgt accatcgaag aacactgcat cggcatttcg caggcgcgcg gcgagcgcat
    12481 ccgtcatcac ggcgcaaccg ggaacgtagt aaacgcgctt gcctgcagcg gccaattcga
    12541 cgccgaccgt atgctcgccc tcaaggccca ggtccggctc gccattctcg agaaagagcg
    12601 gcactttccc agggacggcg aagagccgtg ctccgagccc ttggagcggc gagaacgtct
    12661 cctcgatctc gatggtcctt ttcgaaacca gttccgggtc gaggacctga aagaccggat
    12721 tgtcggcgac gattctgccg actgcgccgg tcgaaaacaa ggcgaatgcc tgcttctccc
    12781 gcaggacgag aagtcctgcc agatgatcga tgtcgccatt cgtcaacact acgctttcga
    12841 tagggctgtg gcgcagacgg cgcggctgca gcgggcgatt ggcgtgaatt tgctggcgga
    12901 tgtccgggga ggcattgaac acggcccagg cctcgccatc gaggctcact gcaagcgagg
    12961 actgggtctg tggtttgaga ccggagcccg ggtcgcgcgc catcgcgcag ttgggacagc
    13021 cgcaattcca ttgagggaga ccgccgccgg cggcggcccc caagacaagt attcgaagat
    13081 cgggcgtttt gttcaccgga atacctgatg ctttaccgat cctcgtcgcg ctggccgtta
    13141 gaagaggatc ggttcatccc cgtccgcagg agcgtagcgg gtaatttcca tagcgcagct
    13201 gacttcgata aattttggtt tatgccaggc cataattcct ccattcgccc ttgggggtcg
    13261 ttgtgctcgc tcccgttcca gcaggcgccc ttaccgtcgg gggcgggcat gaaccaaaca
    13321 gggcttttgt cggcccatgc ttgggtgaca cgtccggacc ggcatttcgc tgttccggca
    13381 gagcccggcg gcgccgtagc cgggctttca ggcatcgtgc aatctcctct tggggtccat
    13441 ggtagcaagc cgggcagcac cccacaatta cgacctaggt gttggtcgcg gtcctcccgg
    13501 ccgcaccccg tccaactcct gaataccgat cgggttcaca cgctcgagcg gatacgcgct
    13561 gacatcatcg tgatcgggag ccttccccgt ccaaatggaa ggcgctagcc gaacgcgttg
    13621 gccgggtatg gcttgctgac aacgggcaga tagcgagcgc ccgacgacag cacgaattgt
    13681 tcgaaagcct gcattgccgg tgtggccgca cggtccaggc gtgaaaccgt gaaccattgc
    13741 cggcgtatag gcgtgtcgat gacgtcgagt attaccagcc gtccgagctt cacctcctgc
    13801 tcgatcgtgt gggccgagat gaaggcaatc ccaagaccgg caatgaccgc ttgcttgatc
    13861 gtctcgttcg aagcgatctc ggttccgagt tcctccagtt cgtgcggggt atcgctcaga
    13921 aagatctcga gcgaaatacg tgtccccgat cctttttcac gcaccaggaa ctgctcccgc
    13981 gcgatccgct cccgcgaaat ctccagcacg cctgcgagcg ggtgtccggc gggtgctatg
    14041 aagaccagag gatgatcgcc gaatacctgc gcgcgcacct cgaaatcacg cggcggccgc
    14101 cccatcagcg cgatgtcgat ctcgtggtcc cgcagcttgg ctatgatctc ggcgcggttg
    14161 ccgatgaata gatttatctc gacggctgga acctggtcgc ggaaagcggc gatcagttgc
    14221 ggcgcgaaat acttgccggt cgacacgacc ccgaggcgca aacgtccggt tctgagccca
    14281 ctgatggcac cgatcgaatc ctcaagggcg gccaggctgt cctcaaccgc gcgtgccgcc
    14341 tcaagaaagg caaggccata ggccgtcggc accatgccgc cgcgcgtccg gtcgaagagt
    14401 gcaacgccgg tgtcccgttc gagatgctga atctgcaggg tgagcgccgg tccggtcaat
    14461 ccaagtgctt cggcggcaag gttgatcttt cccaaccggc agacggcttc aacggtacgg
    14521 agctggcgga gggacacgtt gcgcatgaag tcacaagtaa ataaaaatta cttatgaagt
    14581 caaatgaata aattttacaa actcgccttt tgcgccatcc tttcccggac atcgtggagg
    14641 agacatcatg tcaggcgcaa ctttagaggc ataccttgct tcatgcacga cccatggcga
    14701 cgagctctca cgggacgtcg ccgccgtgat ccagagactg gcaaaggctg ccctcgacat
    14761 tcgcaaactc gttaatcagg gagcgctcgg caccgtcttc aatggaatgc atagcggcag
    14821 caacacggat ggcgatgttc agaaggacct ggacatcctg tgcgacgacc agttcctctc
    14881 gtgtctgcaa ggcgccccgg tcgcatgcta cgcctcggaa gaactcgaaa atccagttct
    14941 gctcgatccg gctgcacgcc tcgctgttgc catcgatccg ctcgacggct catcgaacat
    15001 cgacaacaac atctccatcg gaacgatatt ttccgtgctt cccgccgcca aggggccgga
    15061 cgttgacccc tcccagtcct tcctgcaacc cggaaaccgg caactggcgg cgggcttctt
    15121 cgtttacggg ccgcaaaccg ctcttgtgct ttcgctcggt agaggcacgg agattttcat
    15181 cttctccagt cggctcggat gttttgtcga tgcgtacaaa tcggtcggca ttccggatcg
    15241 cgcgaacgaa ttcgccatta acatgtccaa ctaccgccat tgggaagaag cgatccggct
    15301 ctatgtcgac gactgcctgg ccggctccga ggggccgcgg gagcgggact tcaacatgcg
    15361 ctggattgcc tcgctcgtcg ccgaagccta tcggatcctc gtccgcggcg ggatcttcct
    15421 ctaccccgcc gacagccgca agggctacag ccatggccgg atacggctgg tctacgaggc
    15481 caacccgatc gccttcatcg tagagaatgc gggcggctcg gccacgacat ccgtcgaccg
    15541 catactcgat ctcgttccag aaagcctgca ccagcgcgtg ccgctggttt tcggttcacg
    15601 ccgcgaggtg gcgcgcatca cccgttatca cgtcgatccg aacatgatcg gcgaacgcgc
    15661 tccgctcttc ggcaagcgcg gtttgttccg ggcctgagga gggcaatatg tcggccaaat
    15721 acccaatcat ctccatcaca ggttcgtccg gcgccggcac cacgaccgtc aaggacacgt
    15781 tcgagaagat attcaagcgc gaaaacatct cggcatcctt tatcgagggc gatgccttcc
    15841 accgctacga ccgagaaacc atgcgcagca agatcgcgga ggaaaaagcc cgaggcgtcg
    15901 acttcacgca tttctccgcc gaggcgaacg aactggagat cctcgaaagc gtctttgccg
    15961 agtacggcag gcgtggcgtc gggcgcaccc gtcactatgt gcacgacgat gccgaggcag
    16021 tgaaattcgg ttcggacccc ggcacgttca cggattggga agagttcaga gacagcgatc
    16081 tcctcttcta tgaaggcctg catggctgcg ccgtcaccga caccatcaac ctcgcccagc
    16141 attgcgatct gaagatcgga gtggtccccg tgatcaatct cgaatggatc cagaagatcc
    16201 atagagacaa ggctacccgc ggttattcca ccgaggccgt gaccgacacc atcctgcggc
    16261 gcatgcccga ctatgtgcac tacatctgcc cgcagttctc cctgacggac atcaacttcc
    16321 aacgcgtacc gatcgtcgac acatccaatc ccttcatcgc ccgctggatc ccgacgccgg
    16381 ccgaatccat cctggtcatc cgttttgcca agccgcaaag catcgatttc ccctacctgc
    16441 tgtcgatgct gcacaacagc tacatgtcgc gcgccaattc catcgtggtg ccgggcgaca
    16501 agctcgatct agccatgcag ctgatcttca ccccgctcat tcacaagctg ctcgagcgca
    16561 agcaccgcat gtcgtgagga ggacatcatg aatgtttcgc agcagatcga accccgcgcc
    16621 gccgcctcgg agcgcaacat ggccgacgcc atccggtttc tttccatgga tgccgtcgag
    16681 aaggccaatt ccggtcatcc gggcatgccg atgggcatgg cggacgcggt taccgtgctc
    16741 ttcaaccgct tcatcagaat cgatccgtcg ctccccgact ggcccgaccg cgaccgtttc
    16801 gtgctttcgg ccggccatgg ctcgatgctg ctctattccc tccatcacct catcggcttt
    16861 gcggacatgc cgatggccga gctttcgtcc ttccggcaac tcggctcgaa aacggccggc
    16921 catcccgaat acggccatgc cctcggcatc gagaccacca ccggcccgct gggccagggg
    16981 atctcgaccg ctgtcgggat ggcgatggcc gaacagatga tggcctcccg gttcggcagt
    17041 gctctgtgca accacttcac ctatgtcgta gccggcgacg gctgccttca ggaaggcatc
    17101 agccacgagg ctatcgacct tgccggacat ttaaagctgc gcaagctggt cgtgctgtgg
    17161 gacgacaacc gaatatcgat cgacggatcg acggatctct ctacctcgat gaaccagctc
    17221 gcgcgtttcc gcgccgccag ctgggacgcc caagccgtcg atggccacga cccggaagcg
    17281 gttgcgaaag ccctggaaag agcacgccgg acccgcaagc cgtcgctgat cgcctgccgc
    17341 acccggatcg gcaagggtgc agccagcatg gaaggctcgc acaaaaccca cggcgcggcg
    17401 ctcggcgaca aggaaatcgc agccacacgc gaaaaacttg gctggccgca tccgcccttc
    17461 ttcgttccgc ctgagataag ggctgcctgg gcaaaggtgg cggctcgagg tcgcacggct
    17521 cgcgaggcct gggatatccg cctcgacgcc tcgcgctcga aaaagcgcta cgagcagacc
    17581 ataaggcggc agtttgacgg cgaactcggc gatctgcttg caaaattccg gagcgcgcat
    17641 cgcacaaggg ctacgaaagt tgcgacgcgt caggcctcgc agatggcgct ggaggtcata
    17701 aacggcgcga ccgctttgac gatcggcggc tcggccgacc tgaccggctc caacctgacg
    17761 atgacctcgc agacccagcc catctcgccg ggcaatttca agggccgtta tctgcattac
    17821 ggcatccgcg agcacggcat ggcggccgct atgaacggca tcgcgcttca tggcggcttc
    17881 atcccctatg gcggcacttt cctggtcttc tccgactatg cccgcggtgc gatgcgcctc
    17941 tcggccctga tgggcctgcc cgtcatttac gtgctgacgc atgattccat cgggctcggc
    18001 gaggacggac cgacccacca gccggtcgag catctggcca tgctgcgcgc cacgcccaac
    18061 ctcaacgttt tccggccggc cgacatcatc gagacggcag aatgctggga gatcgcgctt
    18121 ggcgagaaga atacgccgag cgtcctcgcc ctttcgcgtc aggccctgcc gatgctgcgc
    18181 cggacggaag gcaacgagaa ccagtcggcg ctcggggcgt atgttctgag ggaagcgcgc
    18241 ggcaaccggg acatcacgat ccttgccacg ggatccgaag tcgagatcgc cgtcgctgcc
    18301 gccgagcgcc tgcaggccga ggaaggcatc gcggcggcag tggtctccat gccctgctgg
    18361 gagaagttcg aggttcagga ccttgcctat cggaggaagg tcctcggcga cgcgccccgc
    18421 atcgccatcg aggcggcggg ccggctcggc tgggaccgat ggatggggcc ggacggtgcc
    18481 ttcgtcggca tgaccggctt tggtgcctcg gcaccggcag gcgacctcta ccggcatttc
    18541 ggcattaccg ccgaccatgt cgtcgcagaa gccctggagc ttctccgccg cgcatactcg
    18601 gaaactctgc ccataggtgc ccggatcggt ccgcacccat ccgcacacac cgtcagatca
    18661 tcgcaggagg catgacaatg gcccgcatca cgctccgcca attgctcgat cacgccgccg
    18721 aacgcagcta tggcgtgccg gcattcaaca tcaacaacat ggaacagggg ctggcgatca
    18781 tggaggcggc acgcgcctgc gatgcgcctg tgatccttca ggtctcgcgc ggcgcccgtt
    18841 cctacgccaa cgacatcatg cttgccaaga tgatggaagc gctcgaagtg atgtatccgg
    18901 acatcccgct ctgcatccat caggatcacg gcaacaatgt cgcgacctgc ctcaccgcga
    18961 tccagcacgg cttcacctcc gtgatgatgg acggttcgct gaaggaggat gcgaagacgc
    19021 ctgcagacta cgcctataac gccatgatca cgacggaagt gagccgcctc gcgcacatgg
    19081 tcggcgcctc ggtcgaaggc gaactcggct gcctcggatc gcttgagacc ggccatggcg
    19141 aggcggagga cggtcacggc ttcgaaggcg cgctcgaccg ttcgcaactc ctgaccgatc
    19201 cggacgaagc cgctcgcttc gtcgccgaaa ccggggtcga tgcgctggcg gtcgcaatcg
    19261 gcacctcgca cggcgcctat aagttcaccc gcaagccgac cggcgaagtt ctcgccatgg
    19321 acgtgatcga aaagattcat gagcgcctgc cggatacgca tatcgtcatg cacggctcct
    19381 cctccgtgcc gcaggaatgg caggacgtct tcaatgctca tggcggtgaa atgcgggaga
    19441 catacggcgt gccggtcgaa gagatcgtac ggggcattcg cttcggcgtg cgaaaggtca
    19501 atatcgacac ggatctgcgc ctggcggcgg ccgccgcctt tcgccgcgtc gccgacacga
    19561 gccgcaccga attcgacccg cgcaagtttt tgaaacccgc catggacgca atgtcggcgg
    19621 tctgcaaggc ccggttcgag gccttcggca cggccggaaa cgcgtcccgc atcaaggtcg
    19681 tgcctatggc ggagatggct cgccgctatg caagcggctc cctgaaaccc caggcgaagc
    19741 gggctgaagc cgcctgacca cttccattca gagaaaggaa ccctgttatg aacgctgatg
    19801 caaagacaga gatcaagggc cgggaacgct ataaggccgg cgtgcttaaa tacgcgcaga
    19861 tgggctactg gaacggagac tacgaaccga aggacaccga tctgatcgca ctcttccgga
    19921 tcacgccgca ggatggcgtc gatccgatcg aagcggccgc cgccgttgcc ggcgaaagtt
    19981 cgacagccac ctggaccgtc gtctggacgg atcgcctcac ggcttgcgat caatatcgcg
    20041 ccaaggctta ccgggtggat ccggtaccgg gaacgccagg ccaatatttc tgctacgtcg
    20101 cctacgatct gatcctgttc gaggaagggt cgatcgccaa cctgaccgcg tccatcatcg
    20161 gcaacgtctt ctcgttcaag ccgctgaagg cggcaagact cgaggacatg cgcctgcccg
    20221 tcgcctatgt gaagaccttc aagggtccac cgacgggcat cgtggtcgag cgtgagcggc
    20281 tggacaagtt cggcaaaccg ttgctcggcg ccacgaccaa gccgaagctc gggctttcgg
    20341 gaaagaatta cggccgcgtc gtctatgagg gcctgaaggg cggtctcgac ttcatgaagg
    20401 acgacgagaa catcaattcg cagcccttca tgcattggcg cgaccggtac ctttactgca
    20461 tggaggccgt caaccacgcc tcggccgtca ccggcgaggt caaggggcac tacctcaacg
    20521 tcaccgccgg caccatggag gaaatgtacc ggcgtgccga attcgcaaag gagctcggct
    20581 ccgtcatcgt tatggtcgat ctcatcatcg gctggactgc aattcagtcg atgtcggaat
    20641 ggtgccggca gaatgacatg atcctgcaca tgcaccgggc cggtcacggc acctatacgc
    20701 ggcagaagaa ccacggcatt tcgttccgcg tcatcgccaa atggctgcgg ctcgccggcg
    20761 tcgatcacct tcatgcgggc acggccgtcg gcaagctcga aggcgacccg ttgactgtgc
    20821 agggttacta caatgtctgc cgcgaaatga agaacgcggt cgatcttccg cgcggcctct
    20881 tcttcgagca ggactgggcc gatctcaaga aggtcttgcc ggtggcgtcg ggcggtatcc
    20941 atgctggcca gatgcaccag ctgctggacc ttttcggcga cgacgtcgtg ctgcagttcg
    21001 gcggcggcac gatcgggcat cccatgggca tccaggcagg ggccaccgct aaccgggttg
    21061 cgctggaagc catggtgcta gctcgaaacg agggccgcga catagcccat gaaggccctg
    21121 agatcctgcg ggcggcagcc aaatggtgca agccgctgga agcggccctc gatacctggg
    21181 gcaatatcag cttcaactac accccgaccg acacgtcgga tttcgtgccg agcgtaaccg
    21241 cggcctgacg gtaaagaaga ggagagacga catgcgtatc acccagggat gcttctcgtt
    21301 cctgccggac ctgacggacg agcagataac ggctcaggtg gaatattgcc tcggaagggg
    21361 ctgggctatc ggcgtcgaat acaccgacga cccgcatccc cgtaacacct attgggaaat
    21421 gtggggcaat ccgatgttcg acctgaggga cgcaaaaggc gtgatgatgg aggtggagga
    21481 ttgccgcaag gctcatcccc aggactatat ccgactgaac gccttcgatt ccagccgcgg
    21541 ccttgagacg gtgacgatgt ccttcatcgt caaccgtccc gagaacgaac cgagcctcag
    21601 gatgacccga acggagagcg acggccgcag ccagcactat acgtgggaaa cacagcgata
    21661 ggaggcacga catgctgatg ccggatgctc caacaactac cgaaagcctg ccgacttcgg
    21721 tcgacctcgc tgaggaatac caagcctccg gcgtcgccga gatcctcgac gaactggacc
    21781 gggaactcgt aggattgaaa cccgtcaagc agcgcatccg cgagacggct gccctgcttc
    21841 tggtcgagcg ggctcgccgg gcaatgggcc tcagccatga gccgccatcg ctgcatatga
    21901 gcttcacggg caatcccgga accggcaaga ccaccgtggc gctgcgcatg gccaacctgc
    21961 tgcacaggct cggctacatc cgcaagggcc atctggtttc ggtgacccgg gatgatctgg
    22021 tcggccagta tatcggccac accgcaccca agaccaagga gatcctcaag aaggcgatgg
    22081 ggggcgtgct gttcatcgac gaggcctatt acctctaccg cccggacaat gaacgcgact
    22141 acgggcagga agcgatcgag atccttctgc aggtcatgga gaacaatc
//
