GCF_000017105.1 - 7 region(s) - antiSMASH results

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Location: 559,614 - 602,847 nt. (total: 43,234 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

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Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: peptidase

CBUD_RS03055: methionine aminopeptidase

smcogs: SMCOG1172: methionine aminopeptidase

Lyases: decarboxylase

CBUD_RS03050: Decarboxylase, pyridoxal-dependent

smcogs: SMCOG1180: Decarboxylase, pyridoxal-dependent

Ligases: ligase

CBUD_RS11525: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

CBUD_RS11530: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.66	NRPS	HC-toxin	Alternaria jesenskae
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000342.4	0.68	NRPS	enniatin	Fusarium equiseti
BGC0002135.2	0.67	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001825.4	0.67	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001261.3	0.66	NRPS	AM-toxin	Alternaria alternata
BGC0001166.5	0.66	NRPS	HC-toxin	Alternaria jesenskae
BGC0001135.5	0.65	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0002286.3	0.65	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001240.4	0.64	NRPS	serinocyclin A, serinocyclin B	Metarhizium robertsii
BGC0001132.4	0.64	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.64	NRPS	icosalide A, icosalide B	Burkholderia gladioli

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 24.76 out of 29.38

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 23.38 out of 27.78

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.67 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.88 out of 27.78

Confidence: medium

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.40 out of 21.55

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.73 out of 28.19

Confidence: strong

Additional weak hits (2):

ClgR

Description: Caseinolytic protease gene regulator
Species: Corynebacterium glutamicum ATCC 13032
Score: 21.74 out of 30.22

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 23.56 out of 36.53

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 1, location 559613 - 602847:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Gly)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

CBUD_RS11525: Gly

Search NORINE for peptide: strict or relaxed

AMP-binding (455..862): Gly

nrpys: Gly

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: N/A
Small clusters prediction:: N/A
Single AA prediction:: gly (Gly)

Stachelhaus prediction details:

Stachelhaus sequence:: LQYTFDISGLELQQM-GGEINGYGPSETTIYSTR
Nearest Stachelhaus code(s):: Ala DILQIGQIYK (71% 8Å match)
Stachelhaus code match:: 80% (moderate)

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
FuR variant 1	Ferric Uptake Regulator
MogR	Repressor of flagellar motility genes
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NC_009727.1 - Region 2 - RiPP-like,T1PKS

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Location: 792,582 - 837,420 nt. (total: 44,839 nt) Show pHMM detection rules used

T1PKS: cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or hyb_KS or itr_KS or tra_KS))
RiPP-like: (strepbact or Antimicrobial14 or Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or Lactococcin or Antimicrobial17 or Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxygenase

CBUD_RS04250: monooxygenase FAD-binding

smcogs: SMCOG1050: monooxygenase FAD-binding

Lyases: dehydratase

CBUD_RS04190: L-carnitine dehydratase/bile acid-inducible

smcogs: SMCOG1189: L-carnitine dehydratase/bile acid-inducible

Ligases: ligase synthase

CBUD_RS04110: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

CBUD_RS04195: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

CBUD_RS04200: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002191.2	0.29	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001273.3	0.29	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.29	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.29	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164.3	0.29	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.28	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001284.4	0.28	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002135.2	0.27	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0002749.2	0.27	NRPS, PKS	aspcandine	Aspergillus candidus
BGC0001758.4	0.26	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002191.2	0.63	PKS	prolipyrone B, gibepyrone D	Fusarium graminearum PH-1
BGC0001273.3	0.63	PKS	asperlactone	Aspergillus ochraceus
BGC0001275.3	0.63	PKS	6-methylsalicyclic acid	Glarea lozoyensis
BGC0001276.3	0.63	PKS	6-methylsalicyclic acid	Aspergillus terreus
BGC0001164.3	0.62	PKS	1-heptadecene	Cyanothece sp. PCC 7822
BGC0001160.5	0.62	PKS	1-nonadecene, (14Z)-1,14-nonadecadiene	Synechococcus sp. PCC 7002
BGC0001284.4	0.62	PKS	alternariol	Parastagonospora nodorum SN15
BGC0002135.2	0.61	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001758.4	0.60	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0001641.4	0.60	NRPS	kolossin	Photorhabdus laumondii subsp. laumondii TTO1

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

CcpA

Description: Catabolite control protein A
Species: Streptococcus & Clostridium sp.
Score: 19.77 out of 20.97

Confidence: strong

ArcA

Description: Repressor of carbon oxidation
Species: Escherichia coli str. K-12 substr. MG1655
Score: 19.27 out of 21.55

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.19 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.38 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.35 out of 26.06

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 24.91 out of 28.19

Confidence: strong

CatR

Description: H2O2-responsive repressor
Species: Streptomyces coelicolor
Score: 20.67 out of 25.31

Confidence: medium

Additional weak hits (4):

CopR

Description: Copper-responsive regulator
Species: Lactococcus lactis subsp. lactis Il1403
Score: 23.60 out of 30.97

Confidence: weak

FuR variant 1

Description: Ferric Uptake Regulator
Species: Bacillus, Pseudomonas, Yersinia sp.
Score: 22.26 out of 29.38

Confidence: weak

LuxO

Description: Repressor of luminescence
Species: Vibrio campbellii ATCC BAA-1116
Score: 20.88 out of 26.12

Confidence: weak

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.51 out of 30.25

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 2, location 792581 - 837420:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (ohmal)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

CBUD_RS04195: Asn

Search NORINE for peptide: strict or relaxed

AMP-binding (10..412): Asn

nrpys: Asn

SVM prediction details:

Predicted physicochemical class:: hydrophilic (Arg, Asp, Glu, Asn, Lys, Gln, Orn, Aad)
Large clusters prediction:: Aliphatic chain with H-bond donor (Asp, Asn, Glu, Gln, Aad)
Small clusters prediction:: asp,asn (Asp, Asn)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: YWFSFDFAVTCAKWMIAGEINEYGPTETTVGITT
Nearest Stachelhaus code(s):: Asn DFTKVGEVGK (65% 8Å match)
Stachelhaus code match:: 80% (moderate)

CBUD_RS04200: mal

PKS_AT (622..913): mal

ATSignature: Malonyl-CoA

Top 3 matches:: Malonyl-CoA: 70.8%; inactive: 66.7%

minowa: Malonyl-CoA

Prediction, score:

Malonyl-CoA: 95.0
inactive: 73.3
Methoxymalonyl-CoA: 26.6
Methylmalonyl-CoA: 23.3
Propionyl-CoA: 17.9
CHC-CoA: 12.8
Benzoyl-CoA: 9.2
trans-1,2-CPDA: 0.0
fatty_acid: 0.0
Isobutyryl-CoA: 0.0
Ethylmalonyl-CoA: 0.0
Acetyl-CoA: 0.0
3-Methylbutyryl-CoA: 0.0
2-Methylbutyryl-CoA: 0.0

TFBS definitions

Regulator	Description
ArcA	Repressor of carbon oxidation
CatR	H2O2-responsive repressor
CcpA	Catabolite control protein A
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NC_009727.1 - Region 3 - lanthipeptide-class-ii

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Location: 1,209,782 - 1,232,751 nt. (total: 22,970 nt) Show pHMM detection rules used

lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Hydrolases: hydrolase

CBUD_RS06230: HAD-superfamily hydrolase, subfamily IA, variant

smcogs: SMCOG1115: HAD-superfamily hydrolase, subfamily IA, variant

Lyases: cyclase

CBUD_RS06210: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Isomerases: isomerase

CBUD_RS06235: isochorismate synthase

smcogs: SMCOG1018: isochorismate synthase

Ligases: synthase

CBUD_RS06240: carbamoyl phosphate synthase small subunit

smcogs: SMCOG1249: carbamoyl phosphate synthase small subunit

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002031.3	0.49	other (other)	kitasetaline	Kitasatospora setae KM-6054
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001407.5	0.41	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0001660.3	0.40	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0001210.5	0.40	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.39	ribosomal	thuricin	Bacillus thuringiensis
BGC0002699.2	0.39	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0000517.4	0.38	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002031.3	0.49	other (other)	kitasetaline	Kitasatospora setae KM-6054
BGC0001579.4	0.43	ribosomal	duramycin	Streptomyces cinnamoneus
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001407.5	0.41	ribosomal	bicereucin	Bacillus cereus SJ1
BGC0001660.3	0.40	ribosomal	mathermycin	Marinactinospora thermotolerans
BGC0001210.5	0.40	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442
BGC0000626.3	0.39	ribosomal	thuricin	Bacillus thuringiensis
BGC0002699.2	0.39	ribosomal	nostolysamide A, nostolysamide B	Nostoc punctiforme PCC 73102
BGC0000517.4	0.38	ribosomal	haloduracin β, haloduracin α	Bacillus halodurans C-125

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CBUD_RS06210

allorf_1214590_1214679

Leader:

MRMSMPFS

Core:

PHSRSPISCEKAMRRSWGRSE

Core with dehydrations:

PHDhaRDhaPIDhaCysEKAMRRDhaWGRDhaE

antiSMASH-DB 4.0 matches for allorf_1214590_1214679

100.0%

NC_009727.1and 1 other:

NZ_LK937696.1 Lanthipeptides: allorf_1081979_1082068_lanthipeptide

Lanthipeptides: allorf_1214590_1214679_lanthipeptide

1

PHSRSPISCEKAMRRSWGRSE

21

PHSRSPISCEKAMRRSWGRSE

1

PHSRSPISCEKAMRRSWGRSE

21

28.6%

NZ_MUXN01000027.1:

Lanthipeptides: allorf_019618_019713_lanthipeptide

8

SCEKAMRRSWGRS

20

+C+K + R+ GRS

3

ACQKTISRARGRS

15

27.6%

NZ_BNBV01000031.1:

Lanthipeptides: allorf_32120_32341_lanthipeptide

2

HSRSPISCEKAMRRSWGRS

20

HS P++ A RR W R+

7

HSELPVTGRDAGRRPWPRA

25

with 3 more sequence matches

23.8%

NZ_CP020040.1:

Lanthipeptides: B4U39_RS44905_lanthipeptide

4

RSPISCEKAM

13

R PI+CE ++

4

RPPITCEPSL

13

17.5%

NC_019673.1:

Lanthipeptides: allorf_7149539_7149760_lanthipeptide

2

HSRSP------ISCEKAMRRSWGRS

20

HSRSP I C + + W RS

20

HSRSPSRLVLTIRCTEPVSHDWTRS

44

17.1%

NZ_JACHCP010000005.1:

Lanthipeptides: allorf_349710_349862_lanthipeptide

1

PHSRSPISCEKAMRRSWGR

19

PH+ PI K + S+ R

9

PHAMVPIDTVKQVTASFSR

27

allorf_1222817_1222972

Leader:

MIYNSFNEAGMVAIG

Core:

NVEMPVDMMLGGPMTSVTEAHRANVAVTTCVDRFCT

Core with dehydrations:

NVEMPVDMMLGGPMDhbDhaVDhbEAHRANVAVDhbDhbCysVDRFCysDhb

antiSMASH-DB 4.0 matches for allorf_1222817_1222972

100.0%

NC_009727.1:

Lanthipeptides: allorf_1222817_1222972_lanthipeptide

1

NVEMPVDMMLGGPMTSVTEAHRANVAVTTCVDRFCT

36

NVEMPVDMMLGGPMTSVTEAHRANVAVTTCVDRFCT

1

NVEMPVDMMLGGPMTSVTEAHRANVAVTTCVDRFCT

36

16.7%

NZ_SMBO01000018.1and 1 other:

NZ_SODQ01000013.1 Lassopeptides: EDF57_RS23885_lassopeptide

Lassopeptides: EDF58_RS24350_lassopeptide

4

MPVDMMLGGPMTSV

17

MP+D LG P+ +

5

MPIDEALGWPIAGL

18

16.4%

NZ_KZ819182.1and 3 others:

NZ_KZ819182.1 Lanthipeptides: DBP21_RS35660_lanthipeptideNZ_KZ819182.1 Lanthipeptides: DBP21_RS35660_lanthipeptideNZ_KZ819182.1 Lanthipeptides: DBP21_RS35660_lanthipeptide

Lanthipeptides: DBP21_RS35660_lanthipeptide

3

EMPVDMMLGGPMTSVTE-AHRANVAVTT

29

MP + GGP+ +V E A + +A+ +

21

RMPSGVRCGGPVPAVAEKARKPGLAIAS

48

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Transcription Factor Binding Sites

No hits above weak threshold.

Weak hits (3):

MntR

Description: Manganese transport regulator
Species: Escherichia coli str. K-12 substr. MG1655
Score: 27.51 out of 39.62

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 22.39 out of 28.14

Confidence: weak

LexA variant 3

Description: Repressor of DNA damage response
Species: Escherichia coli str. K-12 substr. MG1655
Score: 23.39 out of 32.93

Confidence: weak

Lanthipeptides

TFBS Finder

Core peptide predictions

allorf_1214590_1214679 - Class II

Cleavage pHMM score: -6.00

RODEO score: 0

Monoisotopic mass: 2366.2 Da

Molecular weight: 2367.7 Da

Number of bridges: 1

Alternative weights: (assuming N unmodified Ser/Thr residues); 2385.7 Da N = 1; 2403.7 Da N = 2; 2421.7 Da N = 3; 2439.7 Da N = 4

allorf_1222817_1222972 - Class II

Cleavage pHMM score: -3.70

RODEO score: 0

Monoisotopic mass: 3773.8 Da

Molecular weight: 3776.4 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 3794.4 Da N = 1; 3812.4 Da N = 2; 3830.4 Da N = 3; 3848.4 Da N = 4

TFBS definitions

Regulator	Description

NC_009727.1 - Region 4 - lanthipeptide-class-ii,terpene-precursor

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Location: 1,327,621 - 1,364,960 nt. (total: 37,340 nt) Show pHMM detection rules used

terpene-precursor: (PT_FPPS_like and not PT_HPP)
lanthipeptide-class-ii: cds(LANC_like and DUF4135)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

Lanthipeptides

Terpene

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: dehydrogenase

CBUD_RS06840: short-chain dehydrogenase/reductase SDR

smcogs: SMCOG1001: short-chain dehydrogenase/reductase SDR

Lyases: cyclase

CBUD_RS06815: lanthionine synthetase C family protein

smcogs: SMCOG1070: lanthionine synthetase C family protein

Isomerases: racemase

CBUD_RS06835: mandelate racemase/muconate lactonizing enzyme

smcogs: SMCOG1268: mandelate racemase/muconate lactonizing enzyme

Ligases: synthetase

CBUD_RS06725: Polyprenyl synthetase

smcogs: SMCOG1182: Polyprenyl synthetase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002276.2	0.19	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000525.5	0.18	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002704.2	0.17	ribosomal	archalan β	Halorussus salinus
BGC0002397.2	0.16	terpene	astallatene	Arabidopsis thaliana
BGC0002400.2	0.14	terpene	boleracene	Brassica oleracea var. oleracea
BGC0000554.4	0.13	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001924.3	0.13	terpene	ascofuranone	Acremonium egyptiacum
BGC0001671.3	0.13	NRPS	monobactam	Agrobacterium tumefaciens
BGC0001229.4	0.13	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0001210.5	0.12	ribosomal	pseudomycoicidin	Bacillus pseudomycoides DSM 12442

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002397.2	0.53	terpene	astallatene	Arabidopsis thaliana
BGC0002276.2	0.52	NRPS	choline	Aspergillus nidulans FGSC A4
BGC0000525.5	0.50	ribosomal	lichenicidin VK21 A1, lichenicidin VK21 A2	Bacillus licheniformis
BGC0002400.2	0.50	terpene	boleracene	Brassica oleracea var. oleracea
BGC0002704.2	0.49	ribosomal	archalan β	Halorussus salinus
BGC0001229.4	0.45	ribosomal	SBI-06990 A1, SBI-06989 A2	Streptomyces bingchenggensis BCW-1
BGC0002398.2	0.44	terpene	(-)-ent-quiannulatene	Arabidopsis thaliana
BGC0001605.3	0.43	terpene	gibberellin	Sinorhizobium fredii NGR234
BGC0000554.4	0.42	ribosomal	SRO15-3108	Streptomyces filamentosus NRRL 15998
BGC0001924.3	0.42	terpene	ascofuranone	Acremonium egyptiacum

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Lanthipeptide predictions

Lanthipeptide(s) for CBUD_RS06815

CBUD_RS06820

Leader:

MSFDNNTFDREVITA

Core:

VGDVEMPMAPKGNLAFPLTVPPVC

Core with dehydrations:

VGDVEMPMAPKGNLAFPLDhbVPPVCys

antiSMASH-DB 4.0 matches for CBUD_RS06820

100.0%

NC_009727.1and 1 other:

NC_011527.1 Lanthipeptides: CBUG_RS03735_lanthipeptide

Lanthipeptides: CBUD_RS06820_lanthipeptide

1

VGDVEMPMAPKGNLAFPLTVPPVC

24

VGDVEMPMAPKGNLAFPLTVPPVC

1

VGDVEMPMAPKGNLAFPLTVPPVC

24

25.9%

NC_009727.1and 1 other:

NC_011527.1 Lanthipeptides: allorf_0704786_0704941_lanthipeptide

Lanthipeptides: allorf_1355034_1355189_lanthipeptide

1

VGDVEMPMAPKGN

13

VG +EMP +GN

1

VGAIEMPAQTRGN

13

16.7%

NZ_JAGPXN010000019.1:

Lanthipeptides: allorf_013625_013804_lanthipeptide

10

PKGNLAFPLTVP

21

P G++A+PLT P

1

PSGDVAWPLTHP

12

with 1 more sequence matches

14.6%

NZ_BBYJ01000018.1:

Lanthipeptides: AWQ71_RS24060_lanthipeptide

2

GDVEMPMAPKGNLAFPLTVPPVC

24

GD+++ A + + +T P C

5

GDIDLSTAAGASNTWWITSTPAC

27

allorf_1355034_1355189

Leader:

MILLTDEVMVMSFNNTFDQEVMAA

Core:

VGAIEMPAQTRGNALSISGHGTMCNIC

Core with dehydrations:

VGAIEMPAQDhbRGNALDhaIDhaGHGDhbMCysNICys

antiSMASH-DB 4.0 matches for allorf_1355034_1355189

100.0%

NC_009727.1and 1 other:

NC_011527.1 Lanthipeptides: allorf_0704786_0704941_lanthipeptide

Lanthipeptides: allorf_1355034_1355189_lanthipeptide

1

VGAIEMPAQTRGNALSISGHGTMCNIC

27

VGAIEMPAQTRGNALSISGHGTMCNIC

1

VGAIEMPAQTRGNALSISGHGTMCNIC

27

25.9%

NC_009727.1and 1 other:

NC_011527.1 Lanthipeptides: CBUG_RS03735_lanthipeptide

Lanthipeptides: CBUD_RS06820_lanthipeptide

1

VGAIEMPAQTRGN

13

VG +EMP +GN

1

VGDVEMPMAPKGN

13

25.9%

NZ_NNBP01000002.1:

Lanthipeptides: allorf_461333_461485_lanthipeptide

10

TRGNALSISGH

20

+RGNA SGH

3

SRGNAKCWSGH

13

Legend:

Dha: Didehydroalanine
Dhb: Didehydrobutyrine

Detailed terpene predictions

Product predictions:

Class	Product name	Chain length	Initial cyclisations	Functional groups
sesquiterpene precursor	FPP	C15	acyclic	PP
diterpene precursor	GGPP	C20	acyclic	PP
sesterterpene precursor	GFPP	C25	acyclic	PP
triterpene precursor	HPP	C30	acyclic	PP

Genes with terpene domains:

CBUD_RS06725: PT_FPPS_like

Domain 1 (29 - 221): Prenyltransferase; Farnesyl diphosphate synthase like

Subtype: unknown

Reactions:: IPP, DMAPP → FPP; IPP, DMAPP → GGPP; IPP, DMAPP → GFPP; IPP, DMAPP → HPP

Transcription Factor Binding Sites

Binding sites found:

IolR

Description: Regulator of the inositol catabolism
Species: Caulobacter crescentus CB15
Score: 20.75 out of 23.79

Confidence: strong

ToxT

Description: Regulator of MSHA biosynthesis
Species: Vibrio cholerae O395
Score: 18.31 out of 18.57

Confidence: strong

GnfM

Description: Regulator of nitrogen-fixation genes
Species: Geobacter sulfurreducens PCA
Score: 21.45 out of 27.55

Confidence: medium

RpoN

Description: Regulator of nitrogen assimilation and virulence
Species: Salmonella & Vibrio sp.
Score: 16.54 out of 18.46

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 21.70 out of 28.19

Confidence: medium

Additional weak hits (6):

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 22.24 out of 32.38

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 22.72 out of 32.93

Confidence: weak

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 22.36 out of 32.93

Confidence: weak

PerR

Description: Peroxide regulator
Species: Bacillus & Staphylococcus sp.
Score: 21.48 out of 28.14

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 22.23 out of 29.65

Confidence: weak

VqsM

Description: Regulator of quorum-sensing signalling systems
Species: Pseudomonas aeruginosa PAO1
Score: 17.08 out of 20.47

Confidence: weak

Lanthipeptides

Terpene

TFBS Finder

Core peptide predictions

CBUD_RS06820 - Class II

Cleavage pHMM score: -4.80

RODEO score: 0

Monoisotopic mass: 2463.2 Da

Molecular weight: 2465.0 Da

Number of bridges: 1

allorf_1355034_1355189 - Class II

Cleavage pHMM score: -3.40

RODEO score: 0

Monoisotopic mass: 2658.3 Da

Molecular weight: 2660.1 Da

Number of bridges: 2

Alternative weights: (assuming N unmodified Ser/Thr residues); 2678.1 Da N = 1; 2696.1 Da N = 2

Glossary

Acronym	Full name
DMAPP	Dimethylallyl diphosphate
FPP	Farnesyl diphosphate
GFPP	Geranylfarnesyl diphosphate
GGPP	Geranylgeranyl diphosphate
HPP	Hexaprenyl diphosphate
IPP	Isopentenyl diphosphate
PP	Diphosphate

TFBS definitions

Regulator	Description
GnfM	Regulator of nitrogen-fixation genes
IolR	Regulator of the inositol catabolism
RpoN	Regulator of nitrogen assimilation and virulence
ToxT	Regulator of MSHA biosynthesis
ZuR variant 1	Zinc-responsive repressor

NC_009727.1 - Region 5 - NRPS,betalactone

Download region SVG

Location: 1,530,118 - 1,574,767 nt. (total: 44,650 nt) Show pHMM detection rules used

NRPS: cds(Condensation and (AMP-binding or A-OX) and not alpha_am_amid)
betalactone: (HMGL-like and AMP-binding)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Transferases: aminotransferase

CBUD_RS07725: aminotransferase

smcogs: SMCOG1019: aminotransferase

Ligases: ligase synthase

CBUD_RS07710: 2-isopropylmalate synthase

smcogs: SMCOG1271: 2-isopropylmalate synthase

CBUD_RS07745: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

CBUD_RS07750: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.90	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.84	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.82	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.80	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.79	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.77	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.77	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.75	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.74	NRPS	AM-toxin	Alternaria alternata
BGC0000307.4	0.74	NRPS	AbT1	Aureobasidium pullulans

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.75	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001833.4	0.73	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.72	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001758.4	0.72	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454
BGC0002286.3	0.71	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.70	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0000307.4	0.69	NRPS	AbT1	Aureobasidium pullulans

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 23.04 out of 29.25

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.10 out of 26.06

Confidence: medium

CodY

Description: Regulator of stationary phase and virulence in Gram-positive bacteria
Species: Bacillus, Lactococcus & Streptococcus sp.
Score: 22.67 out of 27.78

Confidence: medium

AfsR

Description: Pleiotropic regulatory for antibiotic production
Species: Streptomyces coelicolor
Score: 20.11 out of 23.05

Confidence: medium

Additional weak hits (2):

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 21.72 out of 26.06

Confidence: weak

ZuR variant 2

Description: Zinc-responsive repressor
Species: Pseudomonas protegens Pf-5
Score: 25.90 out of 33.14

Confidence: weak

Module view

Legend

Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 8, location 1530117 - 1574767:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Ile) + (Val)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

CBUD_RS07745: Ile - Val

Search NORINE for peptide: strict or relaxed

AMP-binding (1..363): Ile

nrpys: Ile

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: FNAAFDAIVFESFLLFGGEIHVYGPTEATTFSTY
Nearest Stachelhaus code(s):: Ile DAFFLGVTFK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

AMP-binding (1024..1443): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: val (Val)

Stachelhaus prediction details:

Stachelhaus sequence:: LNNSFDASTFEMWLLVGGELNGYCPTEGTTFTTI
Nearest Stachelhaus code(s):: Val DAFWLGGTFK (74% 8Å match)
Stachelhaus code match:: 100% (strong)

TFBS definitions

Regulator	Description
AfsR	Pleiotropic regulatory for antibiotic production
CodY	Regulator of stationary phase and virulence in Gram-positive bacteria
MogR	Repressor of flagellar motility genes
SypG	Regulator of biofilm formation and host colonization

NC_009727.1 - Region 6 - NI-siderophore

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Location: 1,632,633 - 1,662,399 nt. (total: 29,767 nt) Show pHMM detection rules used

NI-siderophore: IucA_IucC

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Translocases: permease

CBUD_RS08260: phenylalanine-specific permease

smcogs: SMCOG1038: phenylalanine-specific permease

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000944.5	0.43	other (non-nrp siderophore)	staphyloferrin A	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002681.3	0.39	other (other)	rhizoferrin	Francisella tularensis subsp. tularensis SCHU S4
BGC0002633.2	0.33	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0001575.4	0.33	NRPS	PZN5, PZN6, PZN7, PZN9, PZN10, PZN11	Ruminococcus sp. 5_1_39BFAA
BGC0002470.2	0.32	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0001314.3	0.27	terpene	tirucalla	Arabidopsis thaliana
BGC0002412.2	0.27	NRPS	holomycin	Photobacterium galatheae
BGC0001869.4	0.27	NRPS, other (phenazine)	griseoluteic acid, demethoxygriseoluteic acid, phenaszentine A, phenaszentine C, phenaszenketide C, phenaszenketide D	Xenorhabdus szentirmaii DSM 16338
BGC0002362.3	0.25	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0000975.5	0.23	NRPS, PKS	Cryptophycin A	Nostoc sp. ATCC 53789

Reference	Similarity score	Type	Compound(s)	Organism
BGC0000944.5	0.43	other (non-nrp siderophore)	staphyloferrin A	Staphylococcus aureus subsp. aureus NCTC 8325
BGC0002681.3	0.39	other (other)	rhizoferrin	Francisella tularensis subsp. tularensis SCHU S4
BGC0002633.2	0.33	other (other)	schizokinen	Leptolyngbya sp. PCC 7376
BGC0001575.4	0.33	NRPS	PZN5, PZN6, PZN7, PZN9, PZN10, PZN11	Ruminococcus sp. 5_1_39BFAA
BGC0002470.2	0.32	other (other)	synechobactin C9, synechobactin C11, synechobactin 13, synechobactin 14, synechobactin 16, synechobactin A, synechobactin B, synechobactin C	Synechococcus sp. PCC 7002
BGC0001314.3	0.27	terpene	tirucalla	Arabidopsis thaliana
BGC0002412.2	0.27	NRPS	holomycin	Photobacterium galatheae
BGC0001869.4	0.27	NRPS, other (phenazine)	griseoluteic acid, demethoxygriseoluteic acid, phenaszentine A, phenaszentine C, phenaszenketide C, phenaszenketide D	Xenorhabdus szentirmaii DSM 16338
BGC0002362.3	0.25	PKS	loseolamycin A1, loseolamycin A2	Micromonospora endolithica
BGC0000975.5	0.23	NRPS, PKS	Cryptophycin A	Nostoc sp. ATCC 53789

Similar known clusters from MIBiG 4.0

No significant matches found.

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

LexA variant 2

Description: Repressor of DNA damage response
Species: Bacillus subtilis subsp. subtilis str. 168
Score: 23.94 out of 24.78

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 23.74 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.24 out of 26.06

Confidence: medium

ZuR variant 1

Description: Zinc-responsive repressor
Species: Streptomyces coelicolor
Score: 22.75 out of 28.19

Confidence: medium

Additional weak hits (4):

ColR

Description: Phenol-responsive regulator
Species: Pseudomonas putida KT2440
Score: 22.16 out of 30.25

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 24.13 out of 32.38

Confidence: weak

HexR

Description: Glucose-responsive regulator
Species: Shewanella oneidensis MR-1
Score: 23.81 out of 31.73

Confidence: weak

LexA variant 1

Description: Repressor of DNA damage response
Species: Streptomyces coelicolor
Score: 20.94 out of 29.65

Confidence: weak

TFBS Finder

TFBS definitions

Regulator	Description
LexA variant 2	Repressor of DNA damage response
MogR	Repressor of flagellar motility genes
ZuR variant 1	Zinc-responsive repressor

NC_009727.1 - Region 7 - NRPS-like,PKS-like

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Location: 1,888,528 - 1,936,986 nt. (total: 48,459 nt) Show pHMM detection rules used

PKS-like: (t2pks2 or ksIII)
NRPS-like: cds((PP-binding or NAD_binding_4) and (AMP-binding or A-OX) and not alpha_am_amid)

Legend:

core biosynthetic genes

additional biosynthetic genes

transport-related genes

regulatory genes

other genes

resistance

binding site

reset view

zoom to selection

Gene details

Select a gene to view the details available for it

Gene overview

Tailoring

MIBiG comparison

KnownClusterBlast

SubClusterBlast

TFBS Finder

NRPS/PKS modules

NRPS/PKS domains

Gene/CDS overview

Filter:

Automatically zoom to filtered/selected features

Identifier	Product	Length		Function	Sequence		NCBI Blast	Filter details
		NT	AA		NT	AA

Tailoring functions

Oxidoreductases: oxidoreductase

CBUD_RS09665: aldo/keto reductase family oxidoreductase

smcogs: SMCOG1039: aldo/keto reductase family oxidoreductase

Hydrolases: esterase

CBUD_RS09645: thioesterase

smcogs: SMCOG1004: thioesterase

Lyases: hydratase

CBUD_RS09625: enoyl-CoA hydratase

smcogs: SMCOG1023: enoyl-CoA hydratase

Ligases: ligase synthase

CBUD_RS09630: hydroxymethylglutaryl-CoA synthase

smcogs: SMCOG1043: hydroxymethylglutaryl-CoA synthase

CBUD_RS09635: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CBUD_RS09640: Beta-ketoacyl synthase

smcogs: SMCOG1022: Beta-ketoacyl synthase

CBUD_RS13325: condensation domain-containing protein

smcogs: SMCOG1127: condensation domain-containing protein

CBUD_RS13330: AMP-dependent synthetase and ligase

smcogs: SMCOG1002: AMP-dependent synthetase and ligase

Similar gene clusters

Analysis type:

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.82	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001825.4	0.79	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.78	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.76	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.75	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.75	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.75	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.73	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.72	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.71	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Reference	Similarity score	Type	Compound(s)	Organism
BGC0002135.2	0.73	NRPS	bovienimide A	Xenorhabdus bovienii SS-2004
BGC0001825.4	0.71	NRPS	xenematide	Xenorhabdus nematophila AN6/1
BGC0001132.4	0.71	NRPS	xenotetrapeptide	Xenorhabdus nematophila ATCC 19061
BGC0001833.4	0.70	NRPS	icosalide A, icosalide B	Burkholderia gladioli
BGC0001128.4	0.70	NRPS	gamexpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0002286.3	0.70	NRPS	ririwpeptide A, ririwpeptide B, ririwpeptide C	Photorhabdus laumondii subsp. laumondii TTO1
BGC0001261.3	0.70	NRPS	AM-toxin	Alternaria alternata
BGC0001135.5	0.69	NRPS	bicornutin A1, bicornutin A2	Xenorhabdus budapestensis
BGC0001166.5	0.69	NRPS	HC-toxin	Alternaria jesenskae
BGC0001758.4	0.69	NRPS	rhizomide A, rhizomide B, rhizomide C	Paraburkholderia rhizoxinica HKI 454

Similar known clusters from MIBiG 4.0

Similar subclusters

No significant matches found.

Transcription Factor Binding Sites

Binding sites found:

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 26.06 out of 26.06

Confidence: strong

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.29 out of 26.06

Confidence: medium

MogR

Description: Repressor of flagellar motility genes
Species: Listeria monocytogenes EGD-e
Score: 22.08 out of 26.06

Confidence: medium

Additional weak hits (8):

RicR

Description: Copper-responsive regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 22.72 out of 32.93

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 19.98 out of 30.12

Confidence: weak

OsdR

Description: Development and stress management regulator
Species: Streptomyces coelicolor
Score: 19.16 out of 30.12

Confidence: weak

CsoR

Description: Copper-responsive repressor
Species: Streptomyces lividans
Score: 20.83 out of 32.38

Confidence: weak

DmdR1

Description: Iron(II)-dependent repressor
Species: Streptomyces coelicolor
Score: 24.28 out of 36.53

Confidence: weak

DosR

Description: Hypoxia stress response regulator
Species: Mycobacterium tuberculosis H37Rv
Score: 20.25 out of 29.51

Confidence: weak

FuR variant 2

Description: Ferric Uptake Regulator
Species: Caulobacter crescentus CB15
Score: 22.66 out of 32.74

Confidence: weak

SypG

Description: Regulator of biofilm formation and host colonization
Species: Vibrio fischeri ES114
Score: 22.42 out of 29.25

Confidence: weak

Module view

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Detailed domain annotation

Selected features only

Show module domains

NRPS/PKS products

NRPS/PKS substrates

TFBS Finder

Predicted core structure(s)

For candidate cluster 10, location 1888527 - 1936986:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 11, location 1888527 - 1929763:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val)

Direct lookup in NORINE database: strict or relaxed

For candidate cluster 12, location 1894502 - 1936986:

Rough prediction of core scaffold based on assumed PKS/NRPS colinearity; tailoring reactions not taken into account

Polymer prediction:: (Val)

Direct lookup in NORINE database: strict or relaxed

NRPS/PKS substrate predictions

CBUD_RS13330: Val

Search NORINE for peptide: strict or relaxed

AMP-binding (1..408): Val

nrpys: Val

SVM prediction details:

Predicted physicochemical class:: hydrophobic-aliphatic (Ala, Gly, Val, Leu, Ile, Abu, Ival, Ser, Thr, Hpg, Dhpg, Cys, Pro, Hpr)
Large clusters prediction:: Apolar, aliphatic (Gly, Ala, Val, Leu, Ile, Abu, Ival)
Small clusters prediction:: Aliphatic, branched hydrophobic (Val, Leu, Ile, Abu, Ival)
Single AA prediction:: N/A

Stachelhaus prediction details:

Stachelhaus sequence:: LENAFDAAIFEGWLLIGGELNAYGPTEGTTFSTI
Nearest Stachelhaus code(s):: Val DAFWLGATFK (71% 8Å match)
Stachelhaus code match:: 100% (strong)