LOCUS       NC_007777              11440 bp    DNA     circular CON 05-SEP-2024
DEFINITION  Frankia casuarinae, complete sequence.
ACCESSION   NC_007777
VERSION     NC_007777.1
KEYWORDS    RefSeq.
SOURCE      Frankia casuarinae
  ORGANISM  Frankia casuarinae
            Bacteria; Actinomycetota; Actinomycetes; Frankiales; Frankiaceae;
            Frankia.
REFERENCE   1  (bases 1 to 5433628)
  AUTHORS   Normand,P., Lapierre,P., Tisa,L.S., Gogarten,J.P., Alloisio,N.,
            Bagnarol,E., Bassi,C.A., Berry,A.M., Bickhart,D.M., Choisne,N.,
            Couloux,A., Cournoyer,B., Cruveiller,S., Daubin,V., Demange,N.,
            Francino,M.P., Goltsman,E., Huang,Y., Kopp,O.R., Labarre,L.,
            Lapidus,A., Lavire,C., Marechal,J., Martinez,M., Mastronunzio,J.E.,
            Mullin,B.C., Niemann,J., Pujic,P., Rawnsley,T., Rouy,Z.,
            Schenowitz,C., Sellstedt,A., Tavares,F., Tomkins,J.P., Vallenet,D.,
            Valverde,C., Wall,L.G., Wang,Y., Medigue,C. and Benson,D.R.
  TITLE     Genome characteristics of facultatively symbiotic Frankia sp.
            strains reflect host range and host plant biogeography
  JOURNAL   Genome Res 17 (1), 7-15 (2007)
   PUBMED   17151343
REFERENCE   2  (bases 1 to 5433628)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C.,
            Glavina,T., Hammon,N., Israni,S., Pitluck,S., Goltsman,E.,
            Martinez,M., Schmutz,J., Larimer,F., Land,M., Kyrpides,N.,
            Lykidis,A., Francino,P., Benson,D.R., Huang,Y., Mastronunzio,J.,
            Bickhart,D., Niemann,J., Rawnsley,T., Tisa,L.S. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (19-JAN-2006) US DOE Joint Genome Institute, 2800 Mitchell
            Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000013345.1-RS_2024_09_05
            Annotation Date                   :: 09/05/2024 02:41:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,755
            CDSs (total)                      :: 4,702
            Genes (coding)                    :: 4,516
            CDSs (with protein)               :: 4,516
            Genes (RNA)                       :: 53
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 186
            CDSs (without protein)            :: 186
            Pseudo Genes (ambiguous residues) :: 0 of 186
            Pseudo Genes (frameshifted)       :: 53 of 186
            Pseudo Genes (incomplete)         :: 162 of 186
            Pseudo Genes (internal stop)      :: 10 of 186
            Pseudo Genes (multiple problems)  :: 37 of 186
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 07:46:07
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2192248
            Orig. end                         :: 2203688
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP000249.1.
            URL -- http://www.jgi.doe.gov
            Source DNA and bacteria available from Louis Tisa
            (lst@patia.unh.edu)
            Contact: Paul Richardson (microbes@cuba.jgi-psf.org)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-PGF
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..11440
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="9"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11440
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11440
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6440](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6440
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            369..983
                     /locus_tag="FRANCCI3_RS26835"
                     /old_locus_tag="Francci3_1859"
     CDS             369..983
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FRANCCI3_RS26835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="Francci3_1859"
                     /product="hypothetical protein"
                     /protein_id="WP_011436295.1"
                     /transl_table=11
                     /translation="MSNEAYIEPEPAKRFVDHAVEEYQRCLASRNADGVPTHLPRASGL
                     LFGVDNRHGITIGDIEFVKNVRDSDADVIAEFEDVIAPQFGDVYRNPGRGFWCSGEDVF
                     QAVKKHEAAGLELIGSIHSHPNWHEIGPQHERTQRLSEKPTRMDEYLFRQSSWPVNLIW
                     YVHAGRQGLAHRVAAWKAGPERCAELDLHIPSQIRDEFGLG"
     gene            1027..2619
                     /locus_tag="FRANCCI3_RS09350"
                     /old_locus_tag="Francci3_1860"
     CDS             1027..2619
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (MITE)
                     MITE0000101: Heterocyclization (75% identity)"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436296.1"
                     /locus_tag="FRANCCI3_RS09350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Francci3_1860"
                     /product="cytochrome P450"
                     /protein_id="WP_166793634.1"
                     /sec_met_domain="p450 (E-value: 2.3e-18, bitscore: 64.3,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MASVQARGGDRMNGTQNGAREGLPPPMVTIKIPAAFRALSGGQRQ
                     VAAKGENIREVFADLNECLPGLIDKIMDGQGSVRRYINVYRNDNDIRGLENLETRVEES
                     DVVWIIPAVAGGSIRPAVQEPKVPEVLPSEFFLNPGPSPHATNAELRSKCPVHQINYPP
                     GSEAYAVLGHRAVAEAFTDSRLSKEAENLPPRFRDRALSSSLLMVRNLGFADPPDHARL
                     RRPISRAFLPNAIADLRPRIQDVVDDLIDTFPAPGEIDLLGAFALPLPLTVICEYLGIP
                     VQDRPLFLEWGYILSQDPFQHAESELKAATEEFTDYFTRLVARRRTDLRKDLLSELVRA
                     ADSDALSERELLSTILLLLIAGHKTVANLIGNGTALLLGHPGQLDLLRTTPELIPAAVE
                     EMLRFEGSAAWASMRVAVEDMRLAGTEIPRGSFVHLLLSSAGHDPEAYDEPERFDVTRS
                     PNHHLAFGHGAHFCVGAPLGRLQGDIAFTTLLRRLPGFELAVRQDEVEWLADSSLSRGL
                     RALPIRVRERLPR"
     gene            2681..3634
                     /locus_tag="FRANCCI3_RS09355"
                     /old_locus_tag="Francci3_1861"
     CDS             2681..3634
                     /EC_number="1.1.1.169"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436297.1"
                     /locus_tag="FRANCCI3_RS09355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Francci3_1861"
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="WP_011436297.1"
                     /transl_table=11
                     /translation="MTARAGGPRIAVVGAGAVGGYLGGRLAGAGHEVEFLARGENLAAL
                     QKNGLRVMDDSGCWSVPDVSASDDPDDIGRVDFVLLCVKTGQLSDAVESLGLMVGPDTA
                     VLTLQNGVDAPGQVAARIGRRRVLPGTIRIAATMAGPGEIRHAGTTCEVSFTAWDGQAS
                     RQVTRLRELFRESQVTVTEPDDIWATLWAKFLMVVPVGSLGAATGGATIGELRSRSGTR
                     QLLIAGMREIYEAGLGLGIFLPDDAVDTAMGLVDRQSAGVTTSLQRDILTGRRSELDAW
                     TGAAVRLAHDAGLSAPVHELLYELLAIRESRAAEAA"
     gene            3764..4861
                     /locus_tag="FRANCCI3_RS09360"
                     /old_locus_tag="Francci3_1862"
     CDS             3764..4861
                     /EC_number="2.6.1.9"
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Aminotran_1_2 (33-349). E-value:
                     5.7e-46. Score: 149.2. Matches aSDomain:
                     nrpspksdomains_FRANCCI3_RS09360_Aminotran_1_2.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_1_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DegT_DnrJ_EryC1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1019: aminotransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF002878.0"
                     /locus_tag="FRANCCI3_RS09360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Francci3_1862"
                     /product="histidinol-phosphate transaminase"
                     /protein_id="WP_011436298.1"
                     /sec_met_domain="Aminotran_1_2 (E-value: 4.4e-44, bitscore:
                     149.2, seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="DegT_DnrJ_EryC1 (E-value: 2.7e-05,
                     bitscore: 21.7, seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVRFAGRDPLRDVTAYRSTEPANTAANEPGRFDLSSNELVLPPL
                     PPLPTVLSVIEDGLSRLARYPDPTARTITEEIAKHLHVSPDAVAVGPGSAGVLQQILLA
                     LCGVGDEVIYAWPGFDAYPLLVAISGATSVHVPLTSTGDHDLDEIRARVGPRTRVILLC
                     SPHNPTGVVIDRHRLDSFLRSLPGDVLTVLDEAYVEFDRGENPPGTPDVLSRHRNIVVL
                     RTFSKAYGLAGLRIGYAAGPERIMVTVRKAGLPFGVTHIAEQAAILSLHSEGELRGRLD
                     TVTVSRDELTAGLRWSGLPVLPSRANFVWLPLAAAADSFAQDLAEAGIRVRPYPGYGVR
                     ISVGAPEAQESLLRSLGRGVPTIWV"
     misc_feature    complement(3765..3779)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.35"
                     /tool="antismash"
     aSDomain        3863..4810
                     /aSDomain="Aminotran_1_2"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_FRANCCI3_RS09360_Aminotran_1_2.1
                     "
                     /evalue="5.70E-46"
                     /label="FRANCCI3_RS09360_Aminotran_1_2.1"
                     /locus_tag="FRANCCI3_RS09360"
                     /protein_end="349"
                     /protein_start="33"
                     /score="149.2"
                     /tool="antismash"
                     /translation="DLSSNELVLPPLPPLPTVLSVIEDGLSRLARYPDPTARTITEEIA
                     KHLHVSPDAVAVGPGSAGVLQQILLALCGVGDEVIYAWPGFDAYPLLVAISGATSVHVP
                     LTSTGDHDLDEIRARVGPRTRVILLCSPHNPTGVVIDRHRLDSFLRSLPGDVLTVLDEA
                     YVEFDRGENPPGTPDVLSRHRNIVVLRTFSKAYGLAGLRIGYAAGPERIMVTVRKAGLP
                     FGVTHIAEQAAILSLHSEGELRGRLDTVTVSRDELTAGLRWSGLPVLPSRANFVWLPLA
                     AAADSFAQDLAEAGIRVRPYPGYGVRISVGAPEAQ"
     gene            5001..6440
                     /locus_tag="FRANCCI3_RS09365"
                     /old_locus_tag="Francci3_1863"
     CDS             5001..6440
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436299.1"
                     /locus_tag="FRANCCI3_RS09365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Francci3_1863"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_236701553.1"
                     /sec_met_domain="TIGR03975 (E-value: 4.1e-152, bitscore:
                     505.7, seeds: 14, tool: rule-based-clusters)"
                     /sec_met_domain="PF04055 (E-value: 1.5e-16, bitscore: 59.3,
                     seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPWQGLDTPSSALGVLGPSVREQAPGWSVDELYGNFRWAEHLMCA
                     SGGAIGVADYGKVAVQVFQGVGDWVFAPALYDVASYRVDEYAQLLDAQGVDAEVPVEMQ
                     RHSRTFIRQLAAEIAADPPDIVGFTSTFMQNVPSLALAREIKRVAPGVLTVIGGSNCDG
                     PQGPALHRNFDQLDFVISGEGERSLPALLRCVAAGASVADIPGLSWRSDGMTVTNPPAE
                     SSVPFGVVPAPDYDGYFQALENSSLGPGIRPMAVLETSRGCWWGEVHQCTFCGLNGSNI
                     NFRSKAPERIAHEVRDLASRHRVLDVVMVDNILDMGYIDKVMPELAALDCDLRIHYEIK
                     SNMTREQLGRLRDANVLFVQPGIESLSSHVLRLMEKGVSSAHNVRMLRDGMDLGLSVTW
                     NILYGFPGETDEDYQSLLRKMASLEHLQPPTGAWRIALERFSPYFDDPSIGFMFRTPAR
                     FYELIYNVPKGELYDLVVRVR"
     gene            complement(6511..8151)
                     /locus_tag="FRANCCI3_RS09370"
                     /old_locus_tag="Francci3_1864"
     CDS             complement(6511..8151)
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436484.1"
                     /locus_tag="FRANCCI3_RS09370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Francci3_1864"
                     /product="IS66 family transposase"
                     /protein_id="WP_011436300.1"
                     /transl_table=11
                     /translation="MSVLSVTDDVTEVAYWRGRAERAEECAEKAEARVGQLQLRVEELS
                     EQVAVLSRMLFGRSSEKTGPSSAVDEKPEDRQDSGGGDAGRPARQRGQRPGSRGHGRRD
                     YSHLQTREEIHDVPEVDRACPGCGVAFTPLGTDDSEQVDWQVVITRIVHRRRRYRRCCT
                     CPGPRTVTAPVPPKPIPKGRFTAGFLARLLYEKYVLGLPLHRIARALAADGLGVAEGTL
                     CGALKDVHGLLGGLDEQIVARNAAAGHVHADETTWRVFERVEGKDGTRWWLWVFVAADT
                     VVFRMDPTRSAAPVEKHFGIDRAAGALSDGRRLVVSSDFYTVYQSLGRVDGVDPLWCWA
                     HIRRYFIRAGDAHPQLRYWADQWVARIGMLYLAHRALAAEQPTTGGYREAAGAFEAALR
                     AIDTARRAEAAIHSLHPAAKKVLATLDREWDGLARHQDFPDLDLDNNAAERALRTPVVG
                     RKNYYGAHAEWAAHLAARVWTIVATAERNGREPLAFLTGYLNACATAGGKAPAGPALEP
                     FLTWQTTTQTGSPPSTDPPQDGPPDGPEP"
     gene            8132..8755
                     /locus_tag="FRANCCI3_RS26070"
     CDS             8132..8755
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="FRANCCI3_RS26070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /protein_id="WP_148214907.1"
                     /transl_table=11
                     /translation="MTDRTDTPPRTHHQRPRYQELHPTYHEEPTTPHQHPADTPPDSRH
                     SHDTATRRNQPLHAIAEYLHLVYLFDGSTRGIAGPLEDDLKQAVRNWAEAYPRSTLLYW
                     IDDQGRVVIEDQRASWPEEVIELDELQSNVYLDLFQGAGWEGVRRRLADGGQVVTDEEA
                     RKILRYFVDRGLAFEDEGRYVSLALEVDPYRRKLVDGREVAASR"
     misc_feature    8321..8323
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            8752..9495
                     /locus_tag="FRANCCI3_RS09380"
                     /old_locus_tag="Francci3_1866"
     CDS             8752..9495
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039872.4"
                     /locus_tag="FRANCCI3_RS09380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Francci3_1866"
                     /product="DUF5825 family protein"
                     /protein_id="WP_011436302.1"
                     /transl_table=11
                     /translation="MSALLDIDGLRALADRHGRRPAGGRPGAAVLVLGADWTGLPAPDE
                     LGSLLRRVPVAGVRLAEAVDLSGLPADVTARSIALVRECSSIGVRLSWSLSLSPDQMGM
                     VRILDHLPPPGKVTVLGEGELPLGHWRSAGSFGLFYFRKGPDFVSVVDQRSEATNRFIV
                     DDAVLIDVLRQGMSGCAWADVTRTPQYAAAARDLVGKGLILRVGDYCVTLPVHMRRWPV
                     GTLLLGGTLAAAGTKKSVLTVDRQL"
     gene            complement(9633..10745)
                     /gene="trpS"
                     /locus_tag="FRANCCI3_RS09385"
                     /old_locus_tag="Francci3_1867"
     CDS             complement(9633..10745)
                     /EC_number="6.1.1.2"
                     /GO_function="GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /GO_function="GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="trpS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011436303.1"
                     /locus_tag="FRANCCI3_RS09385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="Francci3_1867"
                     /product="tryptophan--tRNA ligase"
                     /protein_id="WP_011436303.1"
                     /transl_table=11
                     /translation="MNTDSASIGLDTTDSDPATSALRTSAAQARSAELEELILSNPERF
                     RVLTGDRPTGRLHLGHYFGTLHNRVRLQDLGTEIFLIIADYQVLTDRDVADNLTAHVEE
                     LVLDYLAIGIDPARSTIFTHSAVPALNQLMLPFLSLVSVAELNRNPTVKEEIAHSRQSA
                     VSGLMYTYPVHQAADILFCKGNLVPVGQDQLPHLELARTIARRFNDRYGDGTRLFPEPE
                     ALLSSAPLLLGTDGSKMSKSRRNAVALAATADETARLLKGAKTDSERHITYDPANRPEV
                     SSLLLLASLCQNRHPHQVADDIGSAGAAALKKIVIEAVNDYLAPIRARRADYAEDRSHL
                     RRVLREGNERAGAVADATLAEVRTAMNSHY"
     misc_feature    10843..10852
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
ORIGIN
        1 ccggaagaga tcgcaattcc cgcctctcga tgagtcgagc attgcgctgg ccttgtcatg
       61 cgccatcgcc aggtgtagcg tgtcgctagg cgtcgactga ccgtcagaag acgcgtcaat
      121 tcgtaccgcc cgaatagacc atcggaggtc ttcactttcc gatgccctga attcgtgcac
      181 cccattctcg ggaactcttc accaggtagg cacggaagac ctggtcacgc cggaacaaga
      241 atccggacaa gctaatcggc tgatcggagg ccggagatca tcgtacgtag gcttcaacca
      301 ccagcacgta gcgtactcca ggtcatgaac cgaaggacag gacggccagc ctagggatga
      361 tcacgaggat gtcgaacgaa gcctacatcg agcccgaacc cgcgaagcgc ttcgtcgacc
      421 acgcggtgga ggagtatcag cggtgcctgg cgagtaggaa cgccgatggt gtgccaactc
      481 atctaccgcg tgcctccggc ctcctgttcg gtgtcgacaa tcgccacggg atcacgatcg
      541 gcgacatcga attcgtgaag aatgtgcgcg acagcgacgc ggacgtgata gccgagttcg
      601 aagatgtaat cgcaccgcag ttcggcgacg tgtacaggaa ccccggccgc gggttctggt
      661 gctccggcga ggacgtcttt caggcggtga aaaagcacga ggcggcgggt ctcgagctga
      721 tcggctcgat tcattctcat ccgaactggc atgagatcgg acctcagcat gagcggacgc
      781 agcggttgag cgagaagccg acccggatgg atgagtacct gttccgtcag tcctcctggc
      841 cggtcaacct gatctggtat gtccacgcgg gccgtcaggg cctcgcacac cgggtcgccg
      901 catggaaagc agggccagag cggtgcgcag agctcgacct acacatcccg tcgcagatcc
      961 gggatgagtt cggactggga tgacggtcca ccgcggcggc ctcggaagac acgacgcgtc
     1021 aatcgcgtgg cctcggtaca ggccagagga ggagacagga tgaacggaac gcagaacggc
     1081 gctcgcgagg gtcttccacc gcccatggtt accatcaaga taccagcggc cttccgggcg
     1141 ctgtccggcg ggcagcggca ggtcgctgcc aagggcgaga acattcgcga ggtgttcgcc
     1201 gacctgaacg agtgcctgcc cgggctcatc gacaagatca tggacggcca ggggtcggtc
     1261 cgacggtata tcaacgtcta tcgaaatgac aacgacatca gaggcctgga aaatctcgag
     1321 acccgcgtcg aggaaagtga cgtggtctgg ataatacctg cagtggcggg aggtagcatt
     1381 cgtccggcgg tccaggagcc gaaggtgcca gaggttctgc cctcggagtt cttcctcaat
     1441 cctggcccat caccgcacgc gacgaacgcc gagctcagat cgaaatgccc cgtccaccag
     1501 attaactatc ctccgggaag tgaggcatac gccgttctcg gccacagggc cgtggcggag
     1561 gccttcaccg actcgcgcct gtccaaggaa gcggagaacc tacctcccag gtttcgcgac
     1621 agggctctgt ccagcagcct cctcatggtg cgtaacctgg gattcgcgga ccctcccgac
     1681 cacgcccggc tgaggaggcc gatcagccgg gcatttctgc cgaacgcgat cgccgacctc
     1741 cgcccccgca tccaggatgt cgtcgacgat ctcatcgaca cattccccgc gcccggtgag
     1801 atcgacctcc tcggcgcgtt tgccctgccg cttccactca ctgtcatctg cgagtatctc
     1861 gggatacctg tccaggacag accactcttt ctcgagtggg gttacattct cagccaggat
     1921 cccttccagc acgcggagtc cgagctgaag gccgcgaccg aggaattcac cgactacttc
     1981 acccggctgg tggcccgacg ccgcacggat ctgcggaagg acctgctcag tgagctcgta
     2041 agggcggcag acagtgacgc actcagtgaa cgcgaactcc tctccaccat tctcctcctg
     2101 ctcatcgcgg gccacaagac ggtcgcgaac ctgataggaa atgggactgc tctgctactc
     2161 ggccacccag gccagctcga cctgctccga accactcccg agctgattcc cgccgcggtc
     2221 gaggagatgc tgcgcttcga gggttcggcc gcctgggcat ccatgcgggt cgccgtcgag
     2281 gacatgcggc tcgccggaac agaaattccc agggggagct tcgtgcatct cttgctgtcc
     2341 agcgcggggc acgatccgga ggcatatgac gagccggagc ggttcgacgt cacccgatcc
     2401 ccgaaccacc atctggcttt cggccacggc gcgcacttct gcgttggtgc ccccctcggc
     2461 cggcttcaag gagacatcgc cttcacaacg ctgctgcgcc ggttgccagg attcgagctg
     2521 gcagtccgtc aggacgaggt ggagtggctc gccgacagct cgctcagccg ggggctcaga
     2581 gcgctcccca tccgggtccg ggagcggttg ccgcgatgag caccgtatgc cacggccgcg
     2641 gaattacccc ccgccggtcg tctcccgtga gcgatcagcg atgacggcac gagcaggcgg
     2701 accgaggatc gcggtggtag gcgcgggcgc ggtcggtggg tatctcggcg gccgcctggc
     2761 cggcgccggt cacgaggtgg agttcctcgc ccggggcgag aacctcgccg cgctccagaa
     2821 gaacgggctg cgggtaatgg atgactcagg ttgctggtcg gtgccggatg taagcgcctc
     2881 cgacgaccct gacgacatcg gaagagtcga cttcgttctg ctctgcgtaa agacgggaca
     2941 gctttccgat gccgtcgaat cgctgggcct catggtcggc ccggataccg cggtcctcac
     3001 tctgcagaac ggggtcgacg ctcccggcca ggtcgcggcg aggattggcc gccgtcgcgt
     3061 gctgcccgga acgataagga tcgcggcgac gatggccggt cctggcgaga ttagacacgc
     3121 cggcaccacc tgcgaggtaa gtttcaccgc gtgggacggc caggcatcaa ggcaggtgac
     3181 gcgccttcgt gagctcttcc gagaatccca ggtgacggtt accgagcctg acgatatttg
     3241 ggccaccctg tgggcgaagt tcctgatggt ggtgccggtc ggcagtctcg gagccgccac
     3301 cggcggcgcg accatcggcg aactccgttc tcggagcggt actcgccagc tcctgatcgc
     3361 cggcatgcgg gagatctacg aggccggtct cgggctggga atcttcctgc ccgacgacgc
     3421 cgtggacacg gcgatgggac tggtggacag gcaatcagcc ggggtcacca cctcacttca
     3481 gcgagacatc ctgaccggta ggcggtcgga gctggacgcg tggaccgggg ccgctgtccg
     3541 gctggcccac gacgcaggtc tgagcgcgcc ggttcacgaa ctcctatacg agttgctcgc
     3601 catccgagag tcgcgtgcgg ccgaagcggc ctagaccctg ccgcccaacc tctgcacggg
     3661 gtctcgggat tcgggaggag ggtcaacgag cacagcggac aggtaccagc agaagtgcag
     3721 ggacgcaggc gatgctccct cgtgatctca ggagtcccaa acggtgaaag tgagattcgc
     3781 cggccgtgac ccgctgcggg acgttacggc ctatcggagc acagagcccg cgaacacggc
     3841 ggcaaacgag cccgggcggt tcgacctgtc aagcaacgaa ctcgtgcttc ccccactgcc
     3901 cccactgccc acagtgctct cggtcatcga agatggcctc agccggctgg cccgttaccc
     3961 cgacccgacg gcacggacca tcacagagga gatcgcgaag cacctgcacg tctccccaga
     4021 cgcggtagcg gtgggccccg gaagcgccgg ggtactccaa cagattcttc tcgcactgtg
     4081 cggcgtcggc gacgaggtca tctacgcgtg gccgggcttc gacgcctacc cgctcctggt
     4141 cgccatcagc ggggccacga gcgttcacgt tccgctcacg tctaccggtg atcacgacct
     4201 ggacgagata cgtgcgcggg tcggcccgcg caccagggtg attcttctct gctctcccca
     4261 caatccaacc ggcgtggtca ttgatcgcca tcgtctggac tcctttctgc gctcactgcc
     4321 gggcgacgtc ctcacggtgc tcgacgaggc ctacgtcgag ttcgatcgcg gcgagaatcc
     4381 cccgggtact ccggatgttc tcagccggca ccgcaacatc gtcgtgctcc ggaccttttc
     4441 gaaggcctac gggctagcgg gcctgcggat cggctacgcc gcgggcccgg agcggatcat
     4501 ggtgaccgtc cgcaaggccg gtcttccctt tggggtcacc cacatcgcgg agcaggcggc
     4561 gatcctctcc ctgcacagcg agggtgagct acgcggtcgc ctggacacgg tgaccgtgtc
     4621 gcgagacgaa ctgaccgctg gtctccggtg gtcgggtctg ccggtgctgc cttctcgcgc
     4681 caacttcgtc tggctccccc tggccgctgc ggccgactcc ttcgcgcagg acctggcgga
     4741 ggcaggaatc agggtccgcc cctatccggg gtatggcgta cggatctccg tcggagcacc
     4801 ggaagcacag gagagcctcc tgaggtccct tggccgaggc gtacccacga tctgggtgtg
     4861 aggaccgcgt catctccggc tcggccggca acggatcctg ccggcgccgc gggcgtgggc
     4921 gtctcggcgc acgagcgcgc cgtgtctttc tagcgaccga aattcgaggt gggaatctaa
     4981 tgaggcttct ccttatcgcc atgccatggc agggacttga cacaccgtcc agtgcgctgg
     5041 gagtactcgg gccttccgtc cgcgaacagg cgcccggctg gtccgtggac gagttgtacg
     5101 gaaacttccg gtgggctgag cacctgatgt gcgctagcgg cggagccatc ggcgtggcgg
     5161 actacggcaa ggtggcggtc caggtctttc agggcgtcgg cgactgggtc ttcgctcccg
     5221 cgctgtatga cgtcgcgagc taccgtgtcg acgagtacgc gcagcttctc gacgcgcagg
     5281 gtgtcgacgc ggaggttccc gtcgagatgc agcgacactc ccggacgttc atccggcagc
     5341 tggcggccga gatcgccgcc gatccaccgg acatcgtcgg attcaccagc actttcatgc
     5401 agaacgttcc gtcactcgcg ctcgcgaggg agatcaagag agtcgcgccg ggcgtcctca
     5461 cggtgatcgg cggcagcaac tgcgacgggc cgcaggggcc ggccctgcac cggaacttcg
     5521 atcagctcga cttcgtgatc agtggtgaag gcgaacggtc gctgcccgcg ctgctgaggt
     5581 gtgtcgccgc aggtgcgagt gtcgccgaca tacccggcct cagctggcgg tccgacggga
     5641 tgacggtcac gaacccaccc gccgagtcgt ccgtgccctt cggcgtggtg ccagcccccg
     5701 actacgacgg ctacttccag gcgctcgaga attcgtcgct cggccccggc atccggccga
     5761 tggcggttct cgagacatcc cgtggctgtt ggtggggcga agttcaccag tgcaccttct
     5821 gcggcctgaa cggatcgaac attaactttc ggagcaaggc tcccgaacgc atcgcgcacg
     5881 aggtccggga cctggcgtcg aggcaccgcg ttctcgacgt ggtgatggtc gacaacattc
     5941 tcgacatggg ctacatcgat aaggtgatgc cggagctggc ggccctcgac tgtgatctga
     6001 ggattcacta cgagatcaag tcgaacatga cccgcgagca gctgggccgc ctgagggacg
     6061 cgaacgtgct cttcgtccag ccgggcatcg agagcctgag cagccacgtg cttcggctga
     6121 tggaaaaggg cgtgagttcg gcgcacaacg tgcgtatgct gcgagatggc atggatctcg
     6181 gcctgagcgt cacctggaac atcctgtacg gattccctgg ggagaccgac gaagattatc
     6241 agagcctgct gaggaaaatg gcatcactgg aacacctcca gccgccaacc ggcgcgtggc
     6301 gcattgcact ggagaggttc agtccctatt tcgatgatcc ttccattgga ttcatgttcc
     6361 gcacgccggc gcgcttctat gaacttatct ataatgttcc aaaaggcgag ttgtatgatc
     6421 tcgtcgtaag agttcggtga tggcgtgggt gggcgggccg aaggcccggc cggtgttgcc
     6481 ggtcgggtgg ttttcggctt gtgggtgggg ttagggctcg ggcccgtcgg gtgggccgtc
     6541 ctggggtggg tcggtgctgg gagggctgcc ggtctgggtg gtggtctgcc aggtgaggaa
     6601 gggttcgagg gcggggccgg cgggtgcttt cccgccggct gtggcgcagg cgttcaggta
     6661 gccggtcagg aacgcgaggg gttcacggcc gttacgctcc gcggtggcga cgatggtcca
     6721 gacccgggcg gcgaggtgcg cggcccactc agcgtgtgcg ccgtagtagt tcttccgccc
     6781 gacgaccggg gtccgtagcg ctctctcggc agcattgttg tcaagatcca ggtcggggaa
     6841 gtcctggtgg cgggccagcc cgtcccattc ccggtccagg gtcgccagga ccttcttcgc
     6901 cgccgggtgc aggctgtgga tcgccgcctc cgcgcgccgc gccgtgtcga tcgccctcag
     6961 cgcggcctcg aacgcgccgg cggcctcgcg gtagccgccg gttgtgggct gctcggcggc
     7021 gagggcgcgg tgagcgaggt agagcatccc gatccgggcg acccactggt cggcccagta
     7081 ccgcagttgg gggtgggcgt ccccggcccg gatgaagtac cggcggatgt gtgcccagca
     7141 ccagagcggg tcgactccgt cgacgcggcc cagggactgg tagacggtgt agaagtccga
     7201 cgagacgacg aggcgacgtc cgtcggacag cgccccggcg gcccggtcga tcccgaagtg
     7261 cttctcgacc ggggcagccg agcgggtcgg gtccatccgg aacaccaccg tgtcggcggc
     7321 gacgaacacc cacagccacc agcgggtccc gtccttgccc tcgacccgct cgaacacccg
     7381 ccacgtcgtc tcgtccgcgt ggacatgacc ggcggcggcg ttacgcgcca cgatctgctc
     7441 atcgagcccg ccgagcagtc catgcacgtc cttcaacgcc ccacacagag tgccctcggc
     7501 aacaccgagc ccgtcggcgg ccagcgcccg agcgatccgg tgcaacggca ggcccaggac
     7561 gtacttctcg tagagaaggc gggcgaggaa ccctgcggtg aaccggccct tgggaatcgg
     7621 tttgggtggc accggcgcgg tcactgtccg cggccccgga catgtgcagc accgccgata
     7681 ccgccgccgc cgatgcacga tccgggtgat cacgacctgc cagtcgacct gttcgctgtc
     7741 gtcggtcccc aacggcgtga acgccacccc acacccgggg caggcacggt cgacctcggg
     7801 cacatcatgg atctcctcgc gggtctgcag atgcgagtag tcccgccggc catgcccccg
     7861 gctccccggc cgctgcccgc gttgacgcgc cggccggccg gcatccccac cgcccgaatc
     7921 ctgccgatct tctggtttct catccacagc cgacgacggg ccggtcttct ccgaggaacg
     7981 accgaacagc atccgggaca gcaccgcgac ctgctcgctc aactcctcga cccgcagctg
     8041 cagctgcccg acacgggcct cggctttctc cgcacactcc tcggcccgct cggcacgccc
     8101 acgccagtac gccacctcgg tgacatcatc ggtgacagac agaacagaca caccacccag
     8161 gacacatcac cagcgtcctc ggtatcagga actacacccg acgtatcacg aagagcccac
     8221 aaccccccac cagcatcccg cggacactcc gcccgactcc cgccacagcc acgacaccgc
     8281 cacacggcgg aaccagcccc tccacgccat cgccgaatac ttacatctcg tctacctttt
     8341 cgacggcagt acgcgaggga tcgcggggcc gctggaggac gacctgaagc aggcagtcag
     8401 aaactgggcc gaggcctatc caaggagcac gcttctgtat tggatcgacg accagggccg
     8461 ggtggtcatc gaggaccagc gggcaagctg gcctgaagag gtcatcgagt tggacgagct
     8521 gcaaagcaat gtatacctcg atctcttcca gggagccggg tgggagggcg tgcgccgacg
     8581 gctcgccgac ggcggccaag tcgtgaccga cgaggaagcc cggaagatcc ttcgttactt
     8641 cgtcgaccgc ggtctcgcct tcgaggacga agggcgctac gtgagcttgg cgctcgaggt
     8701 cgacccgtac cgccggaagc ttgtcgacgg cagagaggtc gcggcatccc gatgagcgcc
     8761 ctgctggata tcgacggact cagggcgctg gccgaccgcc acgggcgccg cccggcgggc
     8821 gggcgaccgg gcgccgcggt gctggtcctc ggtgcggact ggaccgggct ccctgctcct
     8881 gacgaactgg gatcgcttct gcggcgtgtg cccgtcgccg gtgtgcggct ggctgaggcc
     8941 gtcgacctgt caggactccc ggctgacgtg accgcgcgaa gcatcgcgct ggttcgcgaa
     9001 tgctcctcga tcggagtgcg gttgtcgtgg tcattgagcc tgagcccgga ccagatgggg
     9061 atggttcgga tactcgatca cctaccgcct ccggggaagg tgaccgtcct gggcgaggga
     9121 gaactccccc tcggccattg gcgatcggcg ggcagttttg gtctgttcta tttccggaag
     9181 gggccggact tcgtctccgt agtcgaccag aggagcgagg ccacgaatcg gttcatcgtc
     9241 gacgacgccg tcctcatcga cgtgctccgg cagggaatgt cgggatgcgc gtgggccgac
     9301 gtgacccgga ctccgcagta tgccgcggcc gcgcgggacc tggtgggcaa gggcttgatc
     9361 ctgcgcgtcg gcgactattg cgtcactcta ccggtgcata tgcgtcgttg gccggtgggg
     9421 acgcttctgc tgggcgggac tctggccgcg gccggcacga agaagtccgt tctgacggtt
     9481 gaccggcagc tctgaccgac tcccgcggcc ctcttcctgg aagccgggcg gccaccacag
     9541 ccgggtcgcc accgaagccg cccggtgcca ggtgtggctc ggcagtctcg ggcgactcgg
     9601 cgccgacccg gccggccggt gaccccagac cgtcagtagt ggctgttcat ggcggtacgc
     9661 acctcggcga gggttgcgtc ggcgacggct cccgctcgtt cgttgccctc gcggagcaca
     9721 cggcgcagat gggagcggtc ctcggcgtag tcggctcggc gagcccggat cggtgccagg
     9781 tagtcgttga ccgcttcgat cacgatcttc ttaagtgcgg cggccccggc ggagccgatg
     9841 tcgtcggcga cctgatgagg gtgccggttc tggcagagcg aagcgagcag gaggagggag
     9901 gacacctcgg gacggttcgc gggatcgtag gtgatgtggc gctcggagtc ggtcttcgct
     9961 cccttgagca gccgggcggt ctcgtcggcg gtcgcagcca gggccacagc gttacgccgg
    10021 ctcttgctca tcttggagcc atccgtgccg aggagaaggg gcgcgctcga caggagcgcc
    10081 tcgggctctg gaaacagtct ggtgccgtcg ccgtagcggt cgttgaagcg gcgggcgatc
    10141 gtgcgggcga gttcgaggtg gggaagctgg tcctggccca ctgggaccag gtttcccttg
    10201 cagaagagaa tgtcggcggc ctggtggacg gggtaggtgt acatcaggcc actgacggcc
    10261 gactgccggg aatgcgcgat ctcctccttg acggtgggat tgcggttcag ctcggcaacg
    10321 gagacaaggc ttagaaaggg cagcatcagc tggttgaggg cggggacggc actgtgtgtg
    10381 aagatcgtgc tgcgtgccgg gtctatgccg atggccaggt agtccaggac cagttcctcc
    10441 acgtgggcgg tcaggttgtc cgctacgtcg cgatcggtca gaacctggta gtcagcaata
    10501 atcaggaaga tctccgtccc gagatcctga aggcgaaccc gattgtgcaa cgtgccgaag
    10561 tagtgcccga gatgtaggcg cccggtagga cggtcacccg tcagtacccg aaaccgctcg
    10621 gggttgctga gaatcagttc ttccaattcc gcgctgcgcg cctgggcggc ggaggttctg
    10681 agcgccgagg tagcggggtc cgagtctgtg gtatctaggc ctatggacgc ggaatcagtg
    10741 ttcacgggat ctcctggaag gtcggtcccg ccgtcgcgat ggcacgagcc cgatccccga
    10801 gacgcgaagg gccgtccatc cgaacggcgc gatcacagct gggaaggcgg ccgctcctac
    10861 tactgagctt taatcttcga ggtcaagctg gttttctgtg tttgtctacg ggcgggtgtg
    10921 aatcgggtag accggctata tgagatatgc gacgggtggc gggttgacgc ccgccgagca
    10981 ggtggaacgt gagcgggtcc ggagggctgc ggccggccgg ttcgccgcgg gggcgagcca
    11041 ggccgaggtc gcccgggagt tccgggtgac cccgaagacc gcgagccgct ggcaccacgc
    11101 ctgggaggcc gagggcgagt ccgggctgcg ctcggccggg ccggggtcgc ggtgccggtt
    11161 ggatgaccgg gacctggccc ggttggaggc ggtcctgcgc cggggaccgg ggccgtacgg
    11221 ctgggaggat cagcggtgga ccctggcccg ggtccgccag gtgatcgcag cagagttcgg
    11281 ggtggactac accctggccg gggtgtggct gctgctggac cggcagggct ggtcgtgcca
    11341 gctccccgcc cgccgcgcgg tcgaacgcga cgacgccgcg gtcgacgcct gggcgcagga
    11401 ggagtggccg gcggtaaaaa gaccgcggcg gcccaggacg
//
