LOCUS       NC_006582              10405 bp    DNA     circular CON 16-JUN-2024
DEFINITION  Shouchella clausii KSM-K16, complete sequence.
ACCESSION   NC_006582
VERSION     NC_006582.1
KEYWORDS    RefSeq.
SOURCE      Shouchella clausii KSM-K16
  ORGANISM  Shouchella clausii KSM-K16
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Shouchella.
REFERENCE   1
  AUTHORS   Kageyama,Y., Takaki,Y., Shimamura,S., Nishi,S., Nogi,Y.,
            Uchimura,K., Kobayashi,T., Hitomi,J., Ozaki,K., Kawai,S., Ito,S. and
            Horikoshi,K.
  TITLE     Intragenomic diversity of the V1 regions of 16S rRNA genes in
            high-alkaline protease-producing Bacillus clausii spp
  JOURNAL   Extremophiles 11 (4), 597-603 (2007)
   PUBMED   17429572
REFERENCE   2
  AUTHORS   Shirai,T., Suzuki,A., Yamane,T., Ashida,T., Kobayashi,T., Hitomi,J.
            and Ito,S.
  TITLE     High-resolution crystal structure of M-protease: phylogeny aided
            analysis of the high-alkaline adaptation mechanism
  JOURNAL   Protein Eng 10 (6), 627-634 (1997)
   PUBMED   9278275
REFERENCE   3
  AUTHORS   Kobayashi,T., Hakamada,Y., Adachi,S., Hitomi,J., Yoshimatsu,T.,
            Koike,K., Kawai,S. and Ito,S.
  TITLE     Purification and properties of an alkaline protease from
            alkalophilic Bacillus sp. KSM-K16
  JOURNAL   Appl Microbiol Biotechnol 43 (3), 473-481 (1995)
   PUBMED   7632397
REFERENCE   4
  AUTHORS   Hakamada,Y., Kobayashi,T., Hitomi,J., Kawai,S. and Ito,S.
  TITLE     Molecular cloning and nucleotide sequence of the gene for an
            alkaline protease from the alkalophilic Bacillus sp. KSM-K16
  JOURNAL   J. Ferment. Bioeng. 78, 105-108 (1994)
REFERENCE   5
  AUTHORS   Takaki,Y., Kageyama,Y., Shimamura,S., Suzuki,H., Nishi,S.,
            Hatada,Y., Kawai,S., Ito,S. and Horikoshi,K.
  TITLE     The complete genome sequence of the alkaliphilic Bacillus clausii
            KSM-K16
  JOURNAL   Unpublished
REFERENCE   6  (bases 1 to 4303871)
  AUTHORS   Takaki,Y., Kageyama,Y., Shimamura,S., Suzuki,H., Nishi,S.,
            Hatada,Y., Kawai,S., Ito,S. and Horikoshi,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-OCT-2003) Contact:Yasushi Kageyama Kao Corporation,
            Biological Science Laboratories; 2606 Akabane, Ichikai-machi,
            Haga-gun, Tochigi 321-3497, Japan URL :http://www.kao.co.jp/e/
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000009825.1-RS_2024_06_16
            Annotation Date                   :: 06/16/2024 13:26:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,355
            CDSs (total)                      :: 4,253
            Genes (coding)                    :: 4,204
            CDSs (with protein)               :: 4,204
            Genes (RNA)                       :: 102
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 49
            CDSs (without protein)            :: 49
            Pseudo Genes (ambiguous residues) :: 0 of 49
            Pseudo Genes (frameshifted)       :: 25 of 49
            Pseudo Genes (incomplete)         :: 37 of 49
            Pseudo Genes (internal stop)      :: 14 of 49
            Pseudo Genes (multiple problems)  :: 21 of 49
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 15:31:20
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 362075
            Orig. end                         :: 372480
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            AP006627.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    47..67
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 22.38"
                     /tool="antismash"
     gene            complement(721..2172)
                     /locus_tag="ABC_RS01840"
                     /old_locus_tag="ABC0332"
     CDS             complement(721..2172)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /locus_tag="ABC_RS01840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0332"
                     /product="spore germination protein"
                     /protein_id="WP_011245193.1"
                     /transl_table=11
                     /translation="MRTTDQNACAAAIKSAFYQSNSVQTLEIPILETDQALVLFIQEIV
                     DQNKLHALIVDPLTRFRLAKQSYPTESVTTWLKDGTPLPNATVEQIKREMMNGQAVVLL
                     KNEQPVLIDCAMWNVRSVSVPLTSSVYEGPASALTESLDVNMNLLRNYFRSPELAIETL
                     EVGENAAKKMAIVSLHGKTNPDLVKEVRERLKQVHVAEFIISQIATDALEGKGFLFPRT
                     MANDRPDACALALAKGKIVLLVEGSPLAILAPSLFFHFFQNQDDYLSEFGRFGARPLRY
                     LYFFIATFFPAITVAIIRFHLGHIPTEVANQLVKTHETLAPYTIELVFVILLLQLIMDG
                     SYRLPGNTIFAVTFIGTMLISDIATNVSLFHPITIVVIGVCYITSFPVLNRGLLSPIFF
                     IRITLIIVAHFFGFAGMFAVTTAVIIHMAHLRSLGIPYLYPFIPFQPDKWHDTIFRPTL
                     TMAINKPTPLPFKKAEAAQNRRGSY"
     gene            2316..2726
                     /locus_tag="ABC_RS01845"
                     /old_locus_tag="ABC0333"
     CDS             2316..2726
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009794374.1"
                     /locus_tag="ABC_RS01845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0333"
                     /product="hypothetical protein"
                     /protein_id="WP_011245194.1"
                     /transl_table=11
                     /translation="MYNGHDYPMRFSPGTFPFPPTQGGPPGPPPTQGGFFPGQGGPPTT
                     STGQGTGAPPGPPPSQAALSTLGAPSVFAVDPGAIAGCLFRYTFIRLNNGESFWFYPTF
                     VGRRSVAGWRWFFFRWVFFGIDTERIASFQCV"
     misc_feature    complement(3077..3090)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 20.37"
                     /tool="antismash"
     gene            3160..3654
                     /gene="ectA"
                     /locus_tag="ABC_RS01850"
                     /old_locus_tag="ABC0334"
     CDS             3160..3654
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245195.1"
                     /locus_tag="ABC_RS01850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0334"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_050748926.1"
                     /transl_table=11
                     /translation="MVQTPVLTLTKPTVEDGAAMWQLAKSSSLDTNSSYKYIMMCEYFD
                     DTCIVAKDGDEVVGFITAFIPPKQQDTLFIWQIGVAASQRGRGLGLDLINKLIEREACK
                     DIRYVEATVTPSNKASQALFRKLARSHQTDCQVSSCFSEQLFPGDSHEQENTFRIGPLR
                     S"
     gene            3684..4964
                     /gene="ectB"
                     /locus_tag="ABC_RS01855"
                     /old_locus_tag="ABC0335"
     CDS             3684..4964
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (25-358). E-value: 1.2e-81.
                     Score: 266.3. Matches aSDomain:
                     nrpspksdomains_ABC_RS01855_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245196.1"
                     /locus_tag="ABC_RS01855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0335"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_011245196.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6.8e-82, bitscore:
                     273.2, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNETMSIFNELESEVRSYCRSFPAVFTKAKGWKMWDEDGTEYIDF
                     FAGAGALNYGHNDEKMKRALIDYIADDGITHSLDMATKPKAAFLKKLNEVILKPRKLNY
                     KVMFPGPTGTNTVESALKLARKVTGRTEIISFTNGFHGMTIGSLSVTGNASKRKGAGIP
                     LTNVVTMPYDKFGDEEVDTLRYLEQFLHDNGSGVDIPAAVILETVQGEGGINAARMEWL
                     QQLERICKKYDILMIIDDVQAGIGRTGTFFSFEEAGITPDIVCLSKSIGGYGLPLALTL
                     FKPELDIWAPGEHNGTFRGNNHAFITATAALSYWEDPSFEQAIKDKSKKIHAFLTKLVD
                     SYPEMDGCVKGRGFMAGIGSKVDGLAGKVAAEAFKRGLIMETAGPEDEVFKLFPPLTIS
                     DEDLEKGFAIIEESVRAVLGVKEPVTT"
     aSDomain        3759..4757
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ABC_RS01855_Aminotran_3.1"
                     /evalue="1.20E-81"
                     /label="ABC_RS01855_Aminotran_3.1"
                     /locus_tag="ABC_RS01855"
                     /protein_end="358"
                     /protein_start="25"
                     /score="266.3"
                     /tool="antismash"
                     /translation="FTKAKGWKMWDEDGTEYIDFFAGAGALNYGHNDEKMKRALIDYIA
                     DDGITHSLDMATKPKAAFLKKLNEVILKPRKLNYKVMFPGPTGTNTVESALKLARKVTG
                     RTEIISFTNGFHGMTIGSLSVTGNASKRKGAGIPLTNVVTMPYDKFGDEEVDTLRYLEQ
                     FLHDNGSGVDIPAAVILETVQGEGGINAARMEWLQQLERICKKYDILMIIDDVQAGIGR
                     TGTFFSFEEAGITPDIVCLSKSIGGYGLPLALTLFKPELDIWAPGEHNGTFRGNNHAFI
                     TATAALSYWEDPSFEQAIKDKSKKIHAFLTKLVDSYPEMDGCVKGRGFMAGI"
     gene            5001..5405
                     /locus_tag="ABC_RS01860"
                     /old_locus_tag="ABC0336"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245197.1"
                     /locus_tag="ABC_RS01860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0336"
                     /product="ectoine synthase"
                     /protein_id="WP_011245197.1"
                     /sec_met_domain="ectoine_synt (E-value: 1e-48, bitscore:
                     162.4, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVVKLADIIGTNRDVDGGNWRSQRIVVESDGMGYSLHDTQIKAG
                     TETHLWYKYHLESVYVIEGEGEVETVKDGKVWPVKQYECYVLDKNDEHLLRAKTDMRMV
                     CVFNPPVTGEEVHDEDGAYPLPEHMKPLNS"
     misc_feature    complement(5469..5486)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 18.49"
                     /tool="antismash"
     gene            5570..6868
                     /locus_tag="ABC_RS01865"
                     /old_locus_tag="ABC0337"
     CDS             5570..6868
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1143: sugar-binding lipoprotein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121398.1"
                     /locus_tag="ABC_RS01865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0337"
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="WP_011245198.1"
                     /transl_table=11
                     /translation="MWKKHAALLSTLGTVTVLAACGGTEGATTSEEKVTIEYWHVNNED
                     WGGKAIKELVEQFNASQDRIEVKESFQPGNYQGLLQNAQAALSGGNPPDVAQIGYNYIA
                     YVDQNVPYVPVSELAANDEEGEAFLNESYLDNIKALGYSNDKETLAGLPYALSNPIMYV
                     NADLLKEAGWNVDELPQTWDEMQEMAKMVKENTGEYGLYVQQPPDSWAEYSLVRSNGGD
                     FLDFSSDEVVSTIGSPESVEVFDMLGRMIEDQHALHVTWEEGIQAFATGKVGMMMTTVG
                     RRAAIEEQSNFDLRSVEIPTFGDKERQVAAGGNALFSFSQDPEKQAAAWEFMKYLSSPE
                     AMEIWVEGTGYLPPVKGLAEDPDTLGPFFDENPIMQTANRQMESIVPWINFPGANGLQA
                     EQAILDARDQIFDGADAESVLKETESKINGYIQ"
     gene            6887..7684
                     /locus_tag="ABC_RS01870"
                     /old_locus_tag="ABC0338"
     CDS             6887..7684
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /locus_tag="ABC_RS01870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0338"
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="WP_011245199.1"
                     /transl_table=11
                     /translation="MSVYINLLPFMKKINDAPAFLQDWGGGVEISTDGPHWHGDTDWAQ
                     EKERFASYAGDIGVHLPIWELNLASVQFPQYAAYSFEAYRTYLEWASTFASHAVLHTHL
                     YNMPLFHREEAQALSKHYIKQLGDIAEQKGIMLLVENVGFHDKMLFDEREFVQLFEEIP
                     AIQALLDVGHATINNWDIANVIEALGTRLSALHLHDNDGENDLHLPIGAGVTRWNEIWQ
                     AVAEQTHSLRLILEYDESASLEVLKRHGNELLQAGKVVGKINV"
     misc_feature    complement(6913..6926)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 21.72"
                     /tool="antismash"
     gene            7681..8583
                     /locus_tag="ABC_RS01875"
                     /old_locus_tag="ABC0339"
     CDS             7681..8583
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1067: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121400.1"
                     /locus_tag="ABC_RS01875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0339"
                     /product="sugar ABC transporter permease"
                     /protein_id="WP_235360804.1"
                     /transl_table=11
                     /translation="MKMKPASGWLQRIKPLLFIAPAMLPIVLFIFVPLVRSIYWSFLEW
                     NMVSPTKTWVGLDNYVELFSNREFLNASANTLWYMGMLLVLILFVPYMTAYGVTQVSGR
                     WQHFYRGALFVPHVLSLAVASVIFLWLYNPLIGSVNEILRFFQLPDVNWLNDANWALFA
                     ITLVVAWNTFGYHFIVLLAGILSVPEEVKESARVEGMKSKLGMLRRIVFPLTGPTILFV
                     FVLTTVMGIQFAFVPIQMLTNGGPDQSTSNLVFLTYQYGFQFFRTGLGSATAVLTFLFF
                     VLIILLQAKVLDRRVHYES"
     gene            8573..9382
                     /locus_tag="ABC_RS01880"
                     /old_locus_tag="ABC0340"
     CDS             8573..9382
                     /GO_process="GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1069: putative ABC
                     transporter permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121401.1"
                     /locus_tag="ABC_RS01880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ABC0340"
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="WP_011245201.1"
                     /transl_table=11
                     /translation="MKVNGWHLLLAICLVLSFFPVLTMAATAFLETSQLFAGGLQLLPI
                     PPTLDHFSQVWESIPILRYMGNSFFVATVVTLIQLFTSILAAYGFTQFQFRGREFCFYL
                     VIASIFIPIQVTMLPNYILLSDLELVNTYFGLIVPQIANGIGVFLLRQSFRSVPASLIE
                     SARVDGAGDWRRLWKILFPSVKPSVIALGILFFITTWNEYLWPLLMINDNDLMTMPLAL
                     QEFISAEGGTSWGPMMAVAFLASVPPLAVYLLLQRHIMSSFLHTGVK"
ORIGIN
        1 tcaaagtacc gcaatggcgg acagacatgc gtctgtgcaa accgcattta cgtccacgag
       61 tcgataaaag cgccttttgt agaggctttt gctgctgaaa tgaaaaagct aaaagtaggc
      121 aacggcatgg aaccagacag ccacattggc cctcttatta acgaagcagc ggttgcaaaa
      181 gtgcaaaagc atattgagga tgcacaggca aaaggaggcg aagtgatctt tggccaagaa
      241 gccgatgcca caaatggcta cttccaaacg cctgccctta ttgccaatgc gaccgatgac
      301 atgctttgca tgaaggaaga aacatttgga ccgcttgcgc cgattgccac gtacacgtca
      361 acggaagacg tgattgaaag ggccaaccat tccccttacg gtttagcagc atatgtgtat
      421 tcaaatgacc tatcggaagc tgttacgatt gcggaaaggc ttgaatacgg cattgtcggg
      481 ctgaatgatg gtttgccttc ggtcgcccaa gcgccattcg gcggcatgaa agaaagcggc
      541 ctaggccgtg aaggtggcca ttggggcatt gaagaatatg tagaagtgaa atatatatcg
      601 ttgcaacttt aaagagaacc acccattcct ccgcgggaat gggtggttta atcatatgtt
      661 atttaaagaa aggcgatgct ttgagattcg cctcatccca caattaattc atctcgttcc
      721 tcagtagctg cctcttcgat tctgggcagc ctctgctttt ttaaaaggca gcggcgtcgg
      781 tttgttgatg gccatcgtca aagtagggcg gaaaatcgtg tcatgccatt tatcaggctg
      841 aaacggaata aacggataga gatatggaat gccaagggac cttaaatggg ccatatggat
      901 gatgacggcg gttgtcacag caaacattcc tgcaaagcca aagaaatggg ctacgataat
      961 caacgttatg cgaataaaaa aaatcggaga aagcagcccg cggtttaata caggaaaact
     1021 agttatataa caaacgccaa tgacgacgat ggtaatcgga tggaataggc taacatttgt
     1081 agcaatatcg ctaatgagca ttgtgccgat aaacgtcact gcaaaaatcg tattgccagg
     1141 caagcgatag gagccatcca taataagctg caataaaaga atgacaaaga caagctcgat
     1201 tgtatagggg gcaagggttt catgggtttt cacaagctgg ttagccactt ccgtcggtat
     1261 gtgaccgaga tgaaaacgga tgatcgctac agtaatagct ggaaaaaagg tggcaataaa
     1321 aaaatacaaa taccgaagag gacgtgcgcc aaagcggcca aattcggata agtaatcgtc
     1381 ttggttttgg aagaaatgaa aaaacaggct tggtgccaga atcgccaatg gcgatccttc
     1441 tacgagcaag acaattttcc cttttgccaa tgccaatgcg cacgcatctg ggcggtcgtt
     1501 cgccattgtc cgagggaaca gaaacccttt cccttcgagc gcgtcagttg caatttgcga
     1561 aatgatgaat tcggctacat gcacttgttt aagcctttcg cggacttctt tgacaaggtc
     1621 tggattcgtt tttccgtgaa gcgagacaat cgccattttt tttgcagcgt tttctccgac
     1681 ttctaatgtt tcgatcgcta attccgggct acgaaaataa ttacggagca aattcatgtt
     1741 aacgtcaagg ctttccgtca aagccgatgc tggcccttca tacacgctgc ttgtaagggg
     1801 cacactgacg ctgcggacat tccacatcgc gcaatcgatt aaaacaggct gttcgttttt
     1861 caacaataca actgcttggc cgttcatcat ttcccgtttt atttgttcca cagtggcatt
     1921 tggtagcggg gtgccgtctt tcagccaagt cgtaacagat tcagtcgggt atgactgctt
     1981 tgcgagacgg aaacgagtaa ggggatcaac gattaacgca tgcaacttat tttggtcgac
     2041 aatctcttga atgaatagca caagcgcttg gtctgtctct aaaatcggaa tttccagtgt
     2101 ttgcacgctg tttgattggt aaaaagcact tttaatggcc gcagcacatg cattttgatc
     2161 cgttgtcctc atgccatttc ttccttatct gttgttagtt tccaattagt attaggaatt
     2221 aagacgatgc tatacacaaa aaatggatat tgttcaccat tccatgggca gttgcgtagg
     2281 ctagcaaaaa ggttataaaa aggagttgct cgtttatgta caatgggcat gattacccaa
     2341 tgcgctttag cccaggcacg tttccatttc cgccaacaca aggtggccca cctggacccc
     2401 cgcccacaca aggagggttt ttcccaggcc agggaggccc cccgacgact tccacaggtc
     2461 aaggaacggg ggcgccgcct ggcccgcctc cttcccaagc tgctttaagc actttagggg
     2521 cgccttctgt ttttgctgtt gatcctggag cgatcgcagg ctgcctgttt cgttatacgt
     2581 ttatccgcct aaataacggc gaatcgtttt ggttttaccc tacttttgta ggccggcgct
     2641 ctgtagcagg ctggcgttgg tttttcttcc gttgggtatt ctttggcatt gacactgagc
     2701 gaattgcttc atttcagtgc gtgtgacagt aacatcaatc aaattgattt aaaatgtaga
     2761 cgagaaaaaa tgggaatagg tcgagggtta gttagcatag tccagccaaa cgacgacgta
     2821 tgcgagccct tggcgactgg ctgaaaggag ggggaaccct cctttttttg tttttcataa
     2881 gcaaaaggcg aatatggaag gaaacgtgac ttttaaggag gatatgaaaa ggagcctact
     2941 ctctgctcga cttagcacga ctcagccaca tcaatccaat tatatccaac gattaagcgt
     3001 ttgcatttgt tgccacgccc ccctatcatt atagaaggca aagaaagttg cctatgttct
     3061 ttcataaaat ttttcaaaaa cgaacgttta gaatgatgag agacgggtaa ggaaacagtg
     3121 tctggcaaaa acgaaataat aaacgaaaag gaggctgtta tggtccagac accagtacta
     3181 acgttaacaa agccaaccgt ggaagatggc gcggccatgt ggcaactggc gaaatcgtct
     3241 tcgctcgata caaactcgtc ctacaagtac attatgatgt gcgaatattt tgacgataca
     3301 tgtattgtgg caaaagatgg cgacgaagta gtaggattta ttaccgcttt tattcctcct
     3361 aaacagcaag atacgttgtt tatttggcaa atcggggtag cagcatccca acgggggcga
     3421 gggctcggcc ttgacttgat caataagctg attgagcgag aggcatgcaa ggatatccgt
     3481 tacgtggaag caacggtaac accttcgaac aaagcttcgc aagcgttatt caggaaatta
     3541 gcccgaagcc atcaaacaga ctgccaagtg tctagttgtt tttcggaaca gttatttcct
     3601 ggagatagcc atgaacaaga aaacacgttt cgcattggac cacttcgttc ttaaacgaaa
     3661 aaatgaagga ggaacaacag acagtgaatg aaacaatgag cattttcaat gaattggaat
     3721 cagaagtgcg tagttactgc cgcagcttcc cggctgtttt cacgaaggca aaaggctgga
     3781 aaatgtggga tgaggacgga acggaataca tcgacttctt tgctggagca ggggcgctca
     3841 attatggcca caatgatgaa aaaatgaagc gtgctcttat cgattatatt gccgatgacg
     3901 gaattaccca ctcactcgat atggcaacaa agccaaaagc agcattccta aaaaaattga
     3961 acgaagtgat tttaaagcca cgaaagctca attacaaagt gatgtttcct ggtccaacag
     4021 gcacaaatac agttgaatca gccctgaagc tggctcgcaa ggtgacaggg agaacagaaa
     4081 tcatcagttt tacaaatggc ttccacggaa tgacaatcgg gtcgctttcg gtaacaggga
     4141 atgcttctaa acgaaaaggt gcaggcatcc ctttgactaa tgttgtaacg atgccgtatg
     4201 acaaatttgg agacgaagaa gtcgatacac ttcgttatct cgaacagttt ttacatgaca
     4261 atggctctgg agtagacatt ccggcggccg tcattctcga aactgttcaa ggagagggcg
     4321 gcattaatgc cgcacgtatg gaatggttac agcaattaga gcgcatttgc aagaaatacg
     4381 acattttaat gattattgat gatgttcagg cgggaattgg acgcacaggg acgtttttta
     4441 gcttcgagga agcaggtatt acgcctgaca ttgtctgcct gtctaaatca attggcgggt
     4501 atggattgcc attagctctc acccttttca aacctgaact tgatatttgg gctccgggcg
     4561 agcataatgg cacattccgt ggaaacaacc acgcctttat tacagcaacg gcagcattgt
     4621 cctactggga agacccatcg tttgaacaag cgattaaaga caagtcaaag aaaatccatg
     4681 ctttcttaac gaaacttgtt gactcatatc cagaaatgga tggttgtgta aaaggaaggg
     4741 gctttatggc tggaatcggt tcaaaagtag acggcctagc tggaaaagta gcggctgaag
     4801 catttaagcg cggcttaatc atggaaacgg ccggtccaga agatgaagtc tttaagctgt
     4861 tcccgccgct caccatttca gatgaggact tagaaaaagg ctttgccatt atagaagaaa
     4921 gtgtacgcgc cgtccttggc gtaaaagagc ctgtcaccac ttaaacaagc gttagttact
     4981 taatcattgg aggagagaaa atgaaagtag tcaaactggc agacattatt ggcacgaacc
     5041 gggatgtaga cggcggcaat tggcggagcc agcggattgt tgtcgagtca gacggtatgg
     5101 gttattcttt gcatgacacg caaattaaag ctggcacaga aacgcatctt tggtacaaat
     5161 accatcttga atcggtttac gtgattgaag gggaaggcga agtggagacg gtaaaagatg
     5221 gaaaagtatg gcccgtcaag caatacgaat gttatgtgct cgacaaaaat gatgagcact
     5281 tactgcgggc gaagacggat atgcgcatgg tctgtgtgtt taatccaccc gttactggcg
     5341 aagaagttca tgacgaagac ggtgcctatc ctcttccaga acatatgaag cctttaaact
     5401 catagagcgg gaaaaagaat gaaatcgaca aagggcaatg gctctttgtc ttttttttgt
     5461 ccgcttcctt tacaaacgtt ttacaaaata tttgcttccc tctcattaga aattaatagc
     5521 ctgtctttat actagacatg tatttaacca acaaaggggt ggagagactg tgtggaagaa
     5581 acatgcagcc ttgttatcga cactaggcac ggtcactgtt ttagcagctt gtggcggcac
     5641 ggaaggggca accacgagtg aagagaaagt cacgattgag tattggcatg taaacaacga
     5701 agactggggc ggcaaagcaa ttaaagagct ggtcgaacag tttaatgctt cccaggatcg
     5761 aattgaagta aaggaatctt ttcagcctgg aaattatcaa gggctgttgc aaaatgccca
     5821 ggcggcgcta agtggaggga atccacctga tgtcgcgcaa attggctaca actacatagc
     5881 ttatgtcgac caaaacgtgc cttatgtccc tgtgtccgaa ctagcggcta atgatgaaga
     5941 gggggaagcg tttttaaacg aatcttatct cgacaatatt aaagcgcttg ggtattccaa
     6001 cgataaagaa acattggctg gattgcctta tgccttaagc aatccaatta tgtatgtcaa
     6061 tgcagatttg ctaaaagaag ctggctggaa tgtagacgag ctgccgcaaa catgggatga
     6121 gatgcaggaa atggccaaaa tggtaaagga aaatacaggg gagtacggcc tctatgttca
     6181 acagccccca gatagctggg cggagtactc acttgttcgt tcaaatggcg gggatttcct
     6241 cgattttagt agcgacgaag ttgtatcaac catcggttcc ccggaaagtg tggaagtgtt
     6301 tgatatgctt ggacgcatga ttgaagacca acatgccctc catgtgacgt gggaagaagg
     6361 catacaggca tttgccacag gaaaagtcgg gatgatgatg acaaccgtag gccgccgggc
     6421 tgcgattgaa gaacaaagca actttgattt gcgttccgtc gaaattccaa cctttgggga
     6481 taaagagcgc caagtagctg ctggcgggaa tgcgctgttc tcattttcac aggatccaga
     6541 gaaacaggca gcggcgtggg aatttatgaa gtatttatca agcccagaag ccatggaaat
     6601 ttgggttgaa gggactggtt atctaccgcc ggtcaaggga ctggcagaag atccagatac
     6661 gttaggtcct ttttttgacg aaaatccgat catgcaaacc gctaatcggc aaatggaatc
     6721 gattgtccct tggatcaact ttccgggtgc aaatggtttg caggcagagc aagcgattct
     6781 agatgcaagg gatcaaattt ttgacggtgc cgatgcggaa tcggttttga aagaaacaga
     6841 atcaaaaatt aatggatata ttcaataagc gagaggcgga catacaatga gtgtatatat
     6901 caacttgctt ccttttatga aaaaaattaa cgatgcccca gcttttttac aggattgggg
     6961 tggcggagtt gaaatctcga cagacggccc ccattggcat ggcgataccg attgggctca
     7021 ggagaaagag cggtttgctt cctatgcagg agacattggc gtccacttgc caatttggga
     7081 attaaacttg gcctctgtcc agttccctca atatgcggcc tattcctttg aagcataccg
     7141 tacgtactta gaatgggctt ctacatttgc aagccatgct gtgctccaca ctcatctata
     7201 caacatgcct ttgtttcacc gagaagaagc acaagcttta tcgaagcatt acataaaaca
     7261 gctcggtgat atcgctgaac aaaagggcat catgctattg gttgaaaatg tcggttttca
     7321 cgataagatg ctatttgatg aacgggaatt tgtccaattg ttcgaggaaa ttccggcgat
     7381 tcaagcattg cttgatgtcg gccatgccac aattaacaat tgggatatag caaatgtgat
     7441 tgaagcgcta gggacaaggc tgtctgctct tcatttgcat gataatgacg gcgaaaatga
     7501 cttgcatttg ccaattggcg caggagtgac acgctggaat gagatttggc aggccgttgc
     7561 cgagcaaaca cactctctgc gattaatttt agaatatgac gagtcagcat cactagaagt
     7621 gttgaagcgc cacggcaacg agcttttaca agcaggaaaa gtggttggga agataaacgt
     7681 atgaagatga aaccagcaag cggttggctg cagcggataa agccgctttt gtttatcgct
     7741 cctgcgatgc tgccgattgt tctctttatt ttcgttcctc tagttcgctc gatttattgg
     7801 agctttttag aatggaatat ggttagtcca accaaaacat gggtcggttt agacaattac
     7861 gtcgaattgt tttcaaatcg cgagttttta aacgcatcag cgaatacgct atggtacatg
     7921 ggcatgctgc tcgtcctaat cttgtttgtt ccttacatga cagcctacgg agtcacgcaa
     7981 gtttcagggc ggtggcaaca tttttatcgt ggcgctcttt ttgtaccgca cgtgctgtcc
     8041 cttgcggttg cgtctgtcat cttcttatgg ttatacaatc cattgattgg ctcagtgaat
     8101 gaaattctcc gtttctttca acttcccgat gtcaactggc taaatgatgc caattgggcg
     8161 ctgtttgcga ttacgcttgt cgtggcctgg aatacgtttg gctatcactt tattgtgttg
     8221 cttgctggaa ttttaagtgt ccctgaagaa gtgaaggaat cggcccgtgt agaaggaatg
     8281 aaaagcaaac ttggcatgtt gcgccggatt gtttttccgc ttacaggacc aacaattttg
     8341 ttcgtatttg tgttaacgac cgtaatgggc atccaatttg cttttgtgcc gattcaaatg
     8401 ctaaccaatg gcggcccaga ccaatcaaca agcaatttgg tgttcttaac gtatcagtat
     8461 gggttccagt tttttcgcac aggacttggg tcagcaactg ccgtgttaac gtttttgttt
     8521 tttgttctga tcattttgct tcaggctaaa gtgcttgatc ggagggtcca ttatgaaagt
     8581 taacggatgg catttgttat tggcgatttg cctggtcctt agtttttttc cggttttgac
     8641 aatggcggca accgcttttc ttgaaacaag ccagttgttt gcaggtggtt tgcaattatt
     8701 gccgattccg cctacattag accattttag ccaagtgtgg gagtcgatcc cgattttacg
     8761 ctatatgggc aactctttct tcgtcgcaac agtggttaca ttgatacaac tgttcactag
     8821 catccttgct gcttacgggt ttactcaatt tcaattccgt gggagagagt tttgctttta
     8881 cttggtcatt gcgagcatat tcattcctat tcaagtgacg atgctgccaa actatattct
     8941 gttaagtgat ttagagcttg taaacaccta tttcggttta atagtgccgc aaatcgcaaa
     9001 tgggatcggc gttttcctgt taaggcaatc gtttcggtct gttcctgcca gcctaattga
     9061 gtctgctcgc gtggacgggg caggggattg gcgcaggctt tggaaaattt tattcccgtc
     9121 ggttaagcca tcggtgattg ctctaggcat tctctttttt attacgacat ggaacgaata
     9181 tttatggccg ttactaatga ttaacgacaa cgacttgatg acgatgcctc tcgctttgca
     9241 agaatttata agtgcagaag ggggcacatc gtgggggccg atgatggctg ttgcatttct
     9301 ggcgtccgtc ccgccactcg ctgtgtattt gttgttgcag cgacatatta tgagcagctt
     9361 tttgcataca ggggtgaagt agcgatgaaa gaagataaaa gcattgtgtt tacacaagta
     9421 gagaaaaact ttgggcagac aaatgtcatt ccgccaatgg atttaatgat taacagcggc
     9481 gaacgccttg tgcttcttgg gccttctgga tgcggcaagt ccacattgtt gcgactgatc
     9541 gctggtctgg aacagcctac gtcaggcatg attttaatgg gcgggcaagc aatggcaggg
     9601 gttgacgttg ccgatcgcaa tgtggcaatg gttttccaaa actatgccct ttatccccat
     9661 atgaccgtgt ttgataacat cgctttttcg ctcacgattc gcaaaaagaa aaagcaagac
     9721 atacgagaag cagttgaata cgccgccgga ttggtcgagt taaatccgta tttagggcgc
     9781 aaacctgccg aactgtcagg tggccaacgc caacgggtcg cccttgcccg agcggtcgtt
     9841 aaacaagcgc cgtttttcct gttggatgaa ccgttgtcca atcttgatgc gcttttgcgt
     9901 gtgtccgcac gtaatgactt aattgaactt cataaccgct tgaaacaaac gatggtctat
     9961 gtcacgcacg accaattaga ggcaatgaca attggacagc ggatcgccgt tatgaatggc
    10021 ggtgttatcg aacaaattgg gacgcctgaa gacatttacg aaaagcccaa aacacgattt
    10081 gtcgccactt ttatcggcac gccgccaatg aatatgctgc cagctgatgc atttgctgac
    10141 agggcgatga ttcccggagt tgcagaaatg ggtgtaaggc ccgagcacat tatgtttaag
    10201 aagctcccag aagcgtgtgt ccttcacggg gaatatgttc gcaaggaata cattggttca
    10261 cactatgtcc attacctcac catcaatggc gtcactatcc aagtcgtggc agggacagaa
    10321 tcttatacaa ctgggcaaaa agtcgatctc tcgattccgc cagagcatct tcactttttt
    10381 gatgaagccg gcaatcggat tgaag
//
