LOCUS       NC_003155              10471 bp    DNA     linear   CON 12-JUL-2024
DEFINITION  Streptomyces avermitilis MA-4680 = NBRC 14893, complete sequence.
ACCESSION   NC_003155
VERSION     NC_003155.5
KEYWORDS    RefSeq.
SOURCE      Streptomyces avermitilis MA-4680 = NBRC 14893
  ORGANISM  Streptomyces avermitilis MA-4680 = NBRC 14893
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Ikeda,H., Kazuo,S.Y. and Omura,S.
  TITLE     Genome mining of the Streptomyces avermitilis genome and development
            of genome-minimized hosts for heterologous expression of
            biosynthetic gene clusters
  JOURNAL   J Ind Microbiol Biotechnol 41 (2), 233-250 (2014)
   PUBMED   23990133
REFERENCE   2
  AUTHORS   Ikeda,H., Ishikawa,J., Hanamoto,A., Shinose,M., Kikuchi,H.,
            Shiba,T., Sakaki,Y., Hattori,M. and Omura,S.
  TITLE     Complete genome sequence and comparative analysis of the industrial
            microorganism Streptomyces avermitilis
  JOURNAL   Nat Biotechnol 21 (5), 526-531 (2003)
   PUBMED   12692562
REFERENCE   3
  AUTHORS   Omura,S., Ikeda,H., Ishikawa,J., Hanamoto,A., Takahashi,C.,
            Shinose,M., Takahashi,Y., Horikawa,H., Nakazawa,H., Osonoe,T.,
            Kikuchi,H., Shiba,T., Sakaki,Y. and Hattori,M.
  TITLE     Genome sequence of an industrial microorganism Streptomyces
            avermitilis: deducing the ability of producing secondary metabolites
  JOURNAL   Proc Natl Acad Sci U S A 98 (21), 12215-12220 (2001)
   PUBMED   11572948
REFERENCE   4  (bases 1 to 9025608)
  AUTHORS   Omura,S., Ikeda,H., Ishikawa,J., Hanamoto,A., Takahashi,C.,
            Shinose,M., Takahashi,Y., Horikawa,H., Nakazawa,H., Osonoe,T.,
            Kushida,N., Shiba,T., Sakaki,Y. and Hattori,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-MAR-2002) Contact:S Omura Kitasato University,
            Kitasato Institute for Life Sciences; 1-15-1 Kitasato, Sagamihara,
            Kanagawa 228-8555, Japan URL
            :http://avermitilis.ls.kitasato-u.ac.jp/
COMMENT     ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_000009765.2-RS_2024_03_28
            Annotation Date                   :: 03/28/2024 03:55:34
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 8,040
            CDSs (total)                      :: 7,942
            Genes (coding)                    :: 7,651
            CDSs (with protein)               :: 7,651
            Genes (RNA)                       :: 98
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 293
            CDSs (without protein)            :: 291
            Pseudo Genes (ambiguous residues) :: 0 of 293
            Pseudo Genes (frameshifted)       :: 128 of 293
            Pseudo Genes (incomplete)         :: 220 of 293
            Pseudo Genes (internal stop)      :: 25 of 293
            Pseudo Genes (multiple problems)  :: 77 of 293
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:08:24
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 6495024
            Orig. end                         :: 6505495
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BA000030.4.
            On Mar 29, 2016 this sequence version replaced NC_003155.4.
            This work was done in collaboration with Haruo Ikeda(*1), Jun
            Ishikawa(*2), Akiharu Hanamoto(*3), Chigusa Takahashi(*3), Mayumi
            Shinose(*3), Hiroshi Horikawa(*4), Hidekazu Nakazawa(*4), Tomomi
            Osonoe(*4), Norihiro Kushida(*4), Hisashi Kikuchi(*4), Tadayoshi
            Shiba(*5), Yoshiyuki Sakaki(*6,*7), Masahira Hattori(*1,*7)
            and Satoshi Omura(*1,*3).
            Final finishing process and all annotation were done by H. Ikeda
            and J. Ishikawa.
            *1 Kitasato Institute for Life Sciences, Kitasato University *2
            National Institute of Infectious Diseases
            *3 The Kitasato Institute
            *4 National Institute of Technology and Evaluation *5 School of
            Science, Kitasato University
            *6 Institute of Medical Science, University of Tokyo *7 RIKEN,
            Genomic Sciences Center
            All the annotated genes identified are available from following
            urls.
            http://avermitilis.ls.kitasato-u.ac.jp.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10471
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="29"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10471
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10471
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5471](+)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5471
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(614..2089)
                     /db_xref="GeneID:41542449"
                     /locus_tag="SAVERM_RS27660"
                     /old_locus_tag="SAV_5358"
                     /old_locus_tag="SAVERM_5358"
     CDS             complement(614..2089)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /GO_process="GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:41542449"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Bac_transf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1146: sugar transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065681.1"
                     /locus_tag="SAVERM_RS27660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAV_5358"
                     /old_locus_tag="SAVERM_5358"
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="WP_010986761.1"
                     /sec_met_domain="Bac_transf (E-value: 3.8e-59, bitscore:
                     198.0, seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAERTLPPPGGQPPVHGFPPVSVIPPRDATAGFRFPAGQRTAAR
                     RPHGLPLLAADAAAALTAALALPHPLLTVPPLLLAVLCLNARAALYRPTAVPAVLDELP
                     AVCARIAVGWCALAALVAALSPAHILSAGTLLTGCVLQAAASCAGRGTLHWRRRRTLVR
                     HPAAALVVGPAATARRVAAAFLRRPGCGLRPVGILADDAAGGEGLPELSTGEEAQRAVI
                     QNGVRAVLIVGPVGEHGPLLRALAESGCALWEVDADVPAYDRAGRAAYEGAGRTTRDRA
                     PGERIAGFRCRRLDVRRHLGSPAKRAFDVLVSGVLLLLVGPALLACAVVLRVSDGPKVV
                     FRQERVGKDGRPFTLLKFRTHRPADAHEAATRWSVADERNMSRFCRFLRRTSLDELLQL
                     WNVLRGDMSLVGPRPERPYFVAKFSQLHPGYAARHRMRTGVTGLAQIHGLRGDTSIEDR
                     ARFDNAYIDDWSLWQDICVLLRTAAALVRATGS"
     gene            complement(2086..3327)
                     /db_xref="GeneID:41542450"
                     /locus_tag="SAVERM_RS27665"
                     /old_locus_tag="SAV_5359"
                     /old_locus_tag="SAVERM_5359"
     CDS             complement(2086..3327)
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /db_xref="GeneID:41542450"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1045: glycosyl transferase group 1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132933.1"
                     /locus_tag="SAVERM_RS27665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAV_5359"
                     /old_locus_tag="SAVERM_5359"
                     /product="glycosyltransferase"
                     /protein_id="WP_010986762.1"
                     /sec_met_domain="Glycos_transf_1 (E-value: 5.9e-30,
                     bitscore: 103.0, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYQPATAPSPRVLHVTQPVDGGVARVVTELVRAQRAAGFDVTVAC
                     PGHGLAQAVRELGAGVRHWSATRAPGPSLPGEVRRLARLVEEVRPDVLHAHSAKAGLAA
                     RLAVRGRIPTVFQPHAWSFEAVGGVTAAVATAWERWGARWAARVVCVSEAERARGVRAG
                     VRAHWRVIPNGIDPGRFHPAAVTTVRAALAPLAGLDPAAPLVVCVGRLCRQKGQDVLLE
                     AWSSVRRRVPGARLALVGDGPQERPLRHLAPEGVLFAGAVADAVPWYQAADLVVLPSRW
                     EGMALAPLEAMACRRPVVVTDVAGARESLPPGHREHCLVPAGRPGPLADALTRALLDPL
                     LRESLGHQGRRHVLTAHDVRHSAEAVAALYRELLGTGRTSADPAEHAECADPAEHTQCA
                     DLAEHTECRESIRS"
     misc_feature    3597..3611
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.83"
                     /tool="antismash"
     misc_feature    complement(3798..3813)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            4025..4288
                     /db_xref="GeneID:41542451"
                     /locus_tag="SAVERM_RS27670"
                     /old_locus_tag="SAV_5360"
                     /old_locus_tag="SAVERM_5360"
     CDS             4025..4288
                     /codon_start=1
                     /db_xref="GeneID:41542451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020132936.1"
                     /locus_tag="SAVERM_RS27670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAV_5360"
                     /old_locus_tag="SAVERM_5360"
                     /product="hypothetical protein"
                     /protein_id="WP_010986763.1"
                     /transl_table=11
                     /translation="MKSLKAAAVVAGSMVLAGAVAPAAAHAATEVPPASLTGAVNQLTK
                     GPVNLSPLHADNKGSALGTVKGAADSLTQQGNARLIGGLPLG"
     gene            5001..5471
                     /db_xref="GeneID:41542452"
                     /locus_tag="SAVERM_RS27675"
                     /old_locus_tag="SAV_5361"
                     /old_locus_tag="SAVERM_5361"
     CDS             5001..5471
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:41542452"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325460.1"
                     /locus_tag="SAVERM_RS27675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAV_5361"
                     /old_locus_tag="SAVERM_5361"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_078234534.1"
                     /sec_met_domain="melC (E-value: 2.7e-48, bitscore: 162.6,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MCPRPAVSPTEVTGRRRVLRVLFALGAGVTLAPVLAASGSAGPEE
                     ARFDETYRGRRIVGDRYDAQRSGDAYSGAWHVTVDGRPLHLMRRADGSWMTMVDHYRSY
                     PTVLAAARAAVDELGPTARLGVPHGGGGHMDDSGTPGGRKGAERDHGSGVHA"
     gene            5455..6321
                     /db_xref="GeneID:41542453"
                     /locus_tag="SAVERM_RS27680"
                     /old_locus_tag="SAV_5362"
                     /old_locus_tag="SAVERM_5362"
     CDS             5455..6321
                     /codon_start=1
                     /db_xref="GeneID:41542453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019325459.1"
                     /locus_tag="SAVERM_RS27680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAV_5362"
                     /old_locus_tag="SAVERM_5362"
                     /product="tyrosinase family protein"
                     /protein_id="WP_037651451.1"
                     /transl_table=11
                     /translation="MVYTRKDVSTLTRSERRRLVAALLEVKRRGEYDEFVRMHIKYYVA
                     DGETGLRAAHMAPSFLPWHRMFLLDLERVLRRVDESVTMPYWDWTRSRSRTAAPWTEDL
                     LGGTGRQSDRQVTTGPFAYRHGGWPIKEGITDAEYLMRDLGRSRDPIALPTARDLQGAL
                     DDPVYDTAPWNSTSAKGFRNRLEGWGPGRGAASWRNHNRVHRWVGGHMVSGASVNDPVF
                     WMHHAFVDLLWSRWQQRHQGARYLPEQQPARGDAQRGRIVARHERMPPWDVTPDQLEDH
                     SGIYRYA"
     tRNA            complement(6488..6560)
                     /anticodon=(pos:complement(6501549..6501551),aa:Arg,
                     seq:tct)
                     /db_xref="GeneID:41542454"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="SAVERM_RS27685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="SAV_t50"
                     /old_locus_tag="SAVERM_t50"
                     /product="tRNA-Arg"
     gene            complement(6488..6560)
                     /db_xref="GeneID:41542454"
                     /locus_tag="SAVERM_RS27685"
                     /old_locus_tag="SAV_t50"
                     /old_locus_tag="SAVERM_t50"
     misc_feature    complement(6853..6871)
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 20.4"
                     /tool="antismash"
     gene            6871..8478
                     /db_xref="GeneID:41542455"
                     /locus_tag="SAVERM_RS27690"
                     /old_locus_tag="SAV_5363"
                     /old_locus_tag="SAVERM_5363"
     CDS             6871..8478
                     /codon_start=1
                     /db_xref="GeneID:41542455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016435092.1"
                     /locus_tag="SAVERM_RS27690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="SAV_5363"
                     /old_locus_tag="SAVERM_5363"
                     /product="dynamin family protein"
                     /protein_id="WP_037651453.1"
                     /transl_table=11
                     /translation="MVTLDVRPQLLDALSALRDRVAAASFPLPLAGAPRARANRDELLA
                     QLDDYLVPRLRDPEAPLLAVIGGSTGAGKSTLVNSLVGRRVSEAGVLRPTTRTPVLVCH
                     PEDHHWFSGMRVLPDLTRVWVPHQEPGDDLHALVERGERTLRIETAETLPPGLALLDAP
                     DVDSLVADNRVLAAELICAADIWIMVTTAARYADAVPWYLLRTAKENKATLVTVLDRVP
                     HQVVSEVSRQYGALLAKAGLGDVPRFTVPELPESAWGGGLLPATAVATLRTWLIQQVQD
                     PAARQQAMARTAYGVLDSLKSRMPELAGAAAAQYAAALRLTAAVDGAYDSEYARVKGRL
                     QAGAVLAGDALKRWRAYPLDCTAGELLDALVESLGSLLLCAVTAADERVDEAWRREPAS
                     GAPELTDRDPTLESAEHRIGMAARRWRRVLEEYAEEEVRNLDKSLAPDAEVVAALVATA
                     LLGGRRARAAGEGLAERIGAHGALRLRDRGGRLLSEYVDTTLHIERERRLAPLDALDVH
                     AEPQAELIAALSVLQKER"
     misc_feature    9526..9541
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.28"
                     /tool="antismash"
ORIGIN
        1 gcagcagcgc gcacaccccg gcccacaccc tctgccgtgc cgacgcgggt cccagcgcca
       61 gccccaccgc gcacaccagc ccgaaggaga cgacggtcgc catccccatg atgtcgcccg
      121 gcccgaaggt gccgacggcg cggatgtccc ggcccgtgta cgaggcgccg gtcccggtcg
      181 cgtactggtg cacccccagg gccccctgcc acaccgcgag ccccaccatc gaccagacca
      241 gcagccgcag gtcgcgccgg tcgcggacga gcagcagcac ggcggccggg acgagtacga
      301 agatctgcag atagcgcccg aagccggcga ccgccgcgcc cggcgtcgcg gcccccgtcg
      361 ccgccaccgc gaacccgacc acgggcaggc ccagcaccac ggcggcggta cgcgacaggg
      421 ggcgccgccg atgccgcagc agacgtaccg cgcagcacag cacgacgaca ccggacgcgg
      481 cgtcggcgag ggtcgccccg ccctcgcccc ccggcgcgac gggcagcgcg agcagggcga
      541 ccaccgccac caccggcagg acgggcgtca gcccgcgcgc ggcgggcggg gcgcatgcgt
      601 ggctcaccgg cgttcagctc cccgtcgcgc ggaccagcgc ggcggccgtg cgcagcagga
      661 cgcagatgtc ctgccacagg gaccagtcgt cgatgtaggc gttgtcgaag cgggcgcggt
      721 cctcgatgga cgtgtcgccg cgcagcccat ggatctgggc gagcccggtg acgccggtcc
      781 gcatacggtg gcgggcggcg tagccggggt ggagctggct gaacttggcg acgaagtacg
      841 ggcgctcggg gcgcggcccg accaggctca tgtcgccccg caggacgttc cacagctgga
      901 gaagctcgtc gagcgaggtg cggcgcagga agcggcagaa gcggctcatg ttccgctcgt
      961 ccgccacgct ccagcgggtc gcggcctcgt gcgcgtcggc cgggcgatgg gtacggaact
     1021 tcagcagcgt gaaggggcgg ccgtccttgc cgacacgctc ctggcggaag acgaccttcg
     1081 gcccgtcgct gacccgcagt acgaccgcgc aggccagcag cgccgggccg acgagaagga
     1141 gcaggacccc ggagaccagg acgtcgaagg cgcgcttggc gggactgccg aggtgtcggc
     1201 ggacatccag gcggcggcag cggaagccgg cgatccgctc gcccggggcc cggtcgcggg
     1261 tcgtgcgccc ggccccttcg tacgcggctc tccccgcccg gtcgtacgcc ggtacgtccg
     1321 cgtcgacctc ccacagcgcg cagcccgact cggccaacgc ccgcagcagt gggccgtgtt
     1381 cgccgaccgg gcccacgatg agcacggccc gcacgccgtt ctggatgacg gcccgctgcg
     1441 cctcctcgcc cgtgctgagc tccggcagcc cctcgccgcc ggccgcgtcg tcggcgagga
     1501 tgcccaccgg cctgaggccg catccggggc ggcgcagaaa ggcggcggcc acgcgccggg
     1561 cggtggcggc cgggccgacg acgagggccg cggcggggtg acggaccagc gtccggcggc
     1621 ggcgccagtg cagcgtgccg cggcccgcgc agctcgccgc cgcctggagg acgcagccgg
     1681 tcagcagcgt gccggcggac aggatgtggg ccggggagag cgccgcgacc agagccgcga
     1741 gcgcgcacca gcccaccgcg atccgggcgc agacggcggg gagttcgtcc aggacggcgg
     1801 gcacggcggt ggggcggtac agcgccgccc gcgcgttcag acagaggacg gcgagcagca
     1861 gggggggaac ggtcagcagg gggtggggga gcgccagggc ggcggtgagc gcggcggccg
     1921 cgtccgcggc gagcaggggc agcccatggg ggcgccgcgc cgcggtccgc tgcccggcgg
     1981 ggaaccggaa gccggccgtg gcatcgcgcg gggggatgac cgagacgggc gggaatccgt
     2041 gtaccggtgg ctggccgcct gggggaggaa gggtacgttc ggcggtcacg agcggatgga
     2101 ctccctgcac tcggtgtgtt ccgcgaggtc ggcgcactgg gtgtgttccg cggggtcggc
     2161 gcactcggcg tgttccgcgg ggtcggccga agtgcgcccg gtgccgagga gttcgcggta
     2221 gagggccgcg accgcctcgg ccgagtgccg cacgtcgtgc gcggtcagta cgtggcggcg
     2281 cccctggtgg ccgagcgatt cgcgcagcag cggatcgagc agcgccctgg tgagcgcgtc
     2341 ggccagcggg cccggccggc cggcgggcac cagacagtgc tcccggtggc cgggcggcag
     2401 actctcgcgc gccccggcca cgtccgtgac caccacggga cgcctgcacg ccatcgcctc
     2461 cagcggggcc agcgccatgc cctcccagcg ggacggcagc accaccaggt cggcggcctg
     2521 gtaccagggg acggcatcgg cgacggcccc ggcgaacagc acgccctcgg gcgcgaggtg
     2581 acgcagtggc cgctcctgtg gcccgtcccc gacgagcgcc agccgggccc cgggcacccg
     2641 ccgccgcacc gaggaccacg cctcgagcag cacgtcctgc cccttctgcc ggcacagccg
     2701 tcccacgcag accaccagcg gagcggccgg atcgaggccg gcgagcggtg cgagggcggc
     2761 tcgtacggtg gtgacggcgg cggggtggaa gcgccccggg tcgatgccgt tggggatcac
     2821 ccgccagtgg gcgcgtaccc cggcgcgtac gcctcgcgcg cgctccgcct cgctgacgca
     2881 caccacgcgg gcggcccacc gcgcccccca ccgctcccac gccgtggcga ccgccgccgt
     2941 gaccccgccg accgcctcga acgaccaggc gtgcggctgg aacaccgtcg ggatccgccc
     3001 ccgtaccgcc agccgggcgg cgagaccggc cttggcgctg tgggcgtgca gcacgtcggg
     3061 ccggacctcc tcgacgaggc gagcgagccg gcgcacctca ccggggagcg acggtcccgg
     3121 cgcccgcgtg gcggaccagt gccgtacgcc tgcgccgagt tcccgtactg cctgggcgag
     3181 gccgtggccg ggacacgcga ccgtgacgtc gaaaccggcc gcacgctgcg cacgcaccag
     3241 ctcggtgacc acgcgggcga cgccgccgtc caccggctgg gtgacgtgga ggacgcgcgg
     3301 cgaaggggcg gttgctggct ggtacatgcg cggatccctc acgtacgggc ggagccgggg
     3361 gcggtgggag cgagcgtcat cgcttcgcct cgacggcgac gaagagcgct ccggcccgct
     3421 gccacgtggc gccgcacggc gatccggaag gccggctggt caccaccgtg cgggaccacc
     3481 tggaaggcgt ccggcccgcc ctgacggccc gtcggacagg cggaccccag tgcgtgtcgt
     3541 ggtggaagac cccgggtgtg aggcatgacc ggcgttgcct ttcacgagaa acgtcaactt
     3601 ttcggttgat tcgggaacgc gtcactctat cccgctgttc cgtatttacc gggcaagagc
     3661 gacaagtaaa atgaaagcgg atgatttcgt gtcaggtgtc ccgcctggct gttgtgacag
     3721 ccgaggggcc tgccgaactg cggtcggcac caccggcgtc acccaccgcc cgcgacgccc
     3781 acggcgccga ccgcgctcgt gaacaccgtc accgccgacc tgctcggcac cggtccggcg
     3841 catcgctttc tcgcgttcat aagaagatga gaaacgcgga tgagatacgg ccttcattcg
     3901 ggtgaaccct cgtgcggcgt gccggaattt tctatgtacg gggtttccgc gacgttcggg
     3961 gctcgttagg ggcggtgaag caattcccgc tggtgacttc cgagttgccg aagaaaggaa
     4021 cacgatgaag tctctgaagg ctgccgccgt cgttgccggt tccatggttc tggccggtgc
     4081 ggtcgcgccc gccgctgccc acgccgccac ggaggtcccg cccgccagcc tcaccggagc
     4141 ggtgaaccag ctcaccaagg gccccgtcaa cctgtcgccg ctgcacgcgg acaacaaggg
     4201 ttccgcgctc ggcacggtca agggggcggc ggactccctc acccagcagg ggaacgcccg
     4261 gctgatcggc ggacttcccc tgggctgagc ggttcgggcg ctcttcggtc tcaggccttt
     4321 actcggaggc cgtcccgggc tttcactccg gctttctgcc ggacttgcgc ctcaagagcc
     4381 tgtggacgaa ctggggtcga ttccgccgca tgcgggatcg gcccttgctc tcggcttttc
     4441 atggcggccc gtcggtttgt ccggcgggcc gcttcgatgt cacggccttg ccgaggggaa
     4501 acagtaactc cacttgcatt taggggaagt tatgggtcgg cggccctgcc gggcgggcgg
     4561 aggccggagg tgcgggctgt ccggactgct cggcctgttc ggtctgctcg gtcggttcag
     4621 tctgttcggt gttcggtgtt cggtgttcgg tctgttcggc tgtgggtggt cggagaatcc
     4681 ctcattcgtg tggtcgtgtt ggcgcgggtg gtccgctcgg gtgaacgagc gcatgggtat
     4741 gttccggtgc gtcgatacag ttccgcggtg acctcaacca ccagcgaaac gtgccctttt
     4801 cgagccgccg acctgccgcc cctcaaggcc cggtgcgcca cgccggcgga gggaccggcc
     4861 tccttcggtc atccgcgctc accgggtctg actccgggag agccgtggcc gggcctgcgg
     4921 tcgccgaggt cgcgtccggg ggagcggtga ccggggcggt gaacacagcg gccgactccg
     4981 cgagccctgt ggccgactcc atgtgtccgc ggcccgccgt cagccccacg gaggtcacgg
     5041 gacggcggcg ggtgctgcgg gtgctgttcg cgctgggggc cggagtgacg ctggcgccgg
     5101 tcttggcggc gtcggggtcg gccggcccgg aggaggcgcg gttcgacgag acgtatcgcg
     5161 ggcgccgtat cgtcggcgac aggtacgacg cccagcggtc cggggacgcc tactccggcg
     5221 cgtggcacgt caccgtggac gggcggccgc tgcacttgat gcgccgggcc gacggcagct
     5281 ggatgaccat ggtcgaccac taccggtcgt atccgacagt gctcgccgcc gcgcgcgcgg
     5341 ccgtcgacga actcggcccc accgcacggc tgggcgtgcc gcacggcggt ggcggccaca
     5401 tggacgacag cggtacgccg ggcgggcgca agggagccga gagggatcat ggcagtggtg
     5461 tacacgcgta aggacgtcag cacgctcacc cggtccgagc ggcgtcggct cgtcgccgca
     5521 ctgctcgagg tcaaacgccg cggcgagtac gacgagttcg tgcgcatgca catcaagtac
     5581 tacgttgccg acggcgagac cggcctgcgc gccgcccaca tggcaccctc cttcctgccc
     5641 tggcaccgca tgtttctgct ggacctggag cgggtgctgc gccgggtgga cgagtcggtg
     5701 accatgccgt actgggactg gacgcgcagc cgcagccgga cggccgcacc gtggaccgag
     5761 gacctgctcg gcggcaccgg gcggcaatcc gaccgccagg tcaccaccgg ccccttcgcc
     5821 taccgtcacg gtgggtggcc catcaaggag gggatcaccg acgccgagta cctcatgcgt
     5881 gacctgggcc gctcccggga cccgatcgcg ctgccgacgg ccagggatct gcagggcgcc
     5941 ctcgacgatc ccgtctacga cacggccccg tggaactcca cgtccgccaa aggattccgc
     6001 aacaggctgg aggggtgggg tccggggcgg ggagccgctt cgtggcgcaa ccacaaccgg
     6061 gtccaccgtt gggtcggcgg ccacatggtg agcggcgcct ccgtcaacga ccccgtcttc
     6121 tggatgcacc acgccttcgt cgacctcctc tggtcccgct ggcagcagag gcaccagggc
     6181 gcccgctatc tccccgagca gcagcccgcc cggggcgacg cccagcgtgg ccgcatcgtc
     6241 gcgcggcacg agaggatgcc gccgtgggac gtgacaccgg accagctgga ggatcacagc
     6301 gggatctacc ggtacgcgta gcacgtacga gcacggggaa gcccctggcg gtcgcgtacc
     6361 gtcgggggct tctcggctgt ggtgccggct cggacgccgg ttcatcgagg acgcgcttgg
     6421 tctcagcgct gcgcgaaccg gacacgaaga agccccatcg gcgtttccgc tggtgaggct
     6481 gggtttgcgc ccccggcagg actcgaacct gcggccaagc gcttagaagg cgcctgctct
     6541 atccactgag ctacgggggc cgggtgtggt ggcctctgtc ctggtgcccg ggtgtgggcg
     6601 ggatccgcga ccttgccggg acaaggatag ggctcccggg tccttgtccc tggcgcttcc
     6661 cctccgtggc tcgatgtgga ggttcagtga agcggtcctg ataatcgcag gcaggtacga
     6721 atcgtgcacc gcttttggcg tctggcgcac cgggtgttgt gcactcgtta tgcctgcgcc
     6781 ccagtcgtcc cttccgtccc ctgtgtcctg tcggcgcgca gacatgtcta tatgcttcag
     6841 aaagcctcca aaattgggca ttcttcgcat gtggtgacct tggacgtacg gcctcagctg
     6901 ctcgacgcac tctccgccct gcgcgaccgt gtcgccgccg caagctttcc gctgcccctg
     6961 gcgggggctc cacgcgcgcg tgccaaccgc gacgagcttc tcgcgcagct cgacgactac
     7021 ttggtgcccc ggttgaggga ccccgaagcg ccgttgctgg ccgtcatcgg gggatccacc
     7081 ggggccggca agtcgaccct cgtcaactcc ctcgtcgggc gcagggtcag cgaggcgggc
     7141 gtgctgcggc cgaccacccg tacgccggtg ctcgtatgcc atccggagga ccatcactgg
     7201 ttcagcggga tgcgcgtact gcccgacctc acgcgcgtgt gggtgcccca tcaggaaccc
     7261 ggggacgatc tgcacgccct ggtcgaacgg ggggagcgga cgctgcgtat cgagaccgcc
     7321 gagacgctgc cgcccgggct cgccctcctc gacgcgcccg acgtcgactc cctcgtcgcc
     7381 gacaaccgtg tcctcgccgc cgagctgatc tgcgccgcgg acatctggat catggtcacc
     7441 acggccgccc gctacgccga cgcggtgccg tggtatctgc tgcgtaccgc caaggagaac
     7501 aaggcgaccc tggtcaccgt tctcgaccgg gtgccccatc aggtggtgtc cgaggtctcg
     7561 cggcagtacg gggccctgct cgccaaggcc gggctcggcg acgtcccccg gttcaccgtg
     7621 cccgagctgc ccgagtccgc gtggggcggg gggcttcttc cggccaccgc ggtggcgacc
     7681 ctgcggacct ggctcatcca gcaggtgcag gaccccgccg cccggcagca ggccatggcc
     7741 cgtacggcgt acggagtcct cgactcgctc aagtcgcgca tgcccgagct ggccggcgcg
     7801 gcggccgcac agtacgccgc cgcgctgcgc ctcaccgcgg ccgtcgacgg ggcgtacgac
     7861 agcgagtacg cgcgcgtgaa ggggcgtttg caggccgggg ccgtgctcgc gggtgacgcg
     7921 ctgaagcgat ggcgcgccta tccgctggac tgcaccgccg gcgaactgct cgacgcgctc
     7981 gtcgaaagcc tcggctcgct cctgctgtgc gccgtcacgg ccgcggacga gcgcgtcgac
     8041 gaggcctggc ggcgcgagcc cgcctcgggt gccccggagc tgacggaccg tgatcccacg
     8101 ctggagagcg ccgaacaccg gatcgggatg gccgcgcgac gctggcggcg cgtcctcgag
     8161 gagtacgccg aggaagaggt ccgcaacctc gacaagagcc tcgcgccgga cgccgaggtg
     8221 gtggccgccc tcgtcgccac ggcgctgctg ggtggccggc gggcgcgggc cgccggagag
     8281 gggctcgccg agcggatcgg cgcgcacggc gccctcagac tgcgcgaccg gggcggacgg
     8341 ctgctctccg agtacgtcga cacgacactg cacatcgaac gcgagcgccg tctcgccccc
     8401 ctcgacgcgc tcgacgtgca cgccgagcca caggccgaac tcatcgccgc gctgtccgta
     8461 ctgcagaagg agaggtgacc gcggtgactg ccgtcactga ccacacggat cacgccgatc
     8521 atcccgagga cgagcccacg gcggccgagc gcatgggggg tgacggcgcg aggggcgagg
     8581 gcgaaggcac gggagacgta gctacgggag gcgaaggtac gcgggatgaa cccgtgacgg
     8641 accggcccgg gaaggaccga tcggggaagg accggcccgg gaaggatgag ccgacggggg
     8701 agggctccgg cggggacaac gccgccggtg acgcagacgg cgggcacgca cgtgtggagc
     8761 gcgatgcgct tcacacacgc gtgaagcgcg gtttcccgcc cccgtcggcc gccgacgccg
     8821 acgcgtcggg ccgtacggag tccacggaac ccgcggattc cacggagtcc ggcgagccct
     8881 ggggcgacgg gctgatcgcc cggcgcgtgt ccgagacggc cgacgggcaa caggaggtgg
     8941 ccgccctcct ggagaccagg agcaaggccg tccagccgcc cgagcccctc gcgtacgacg
     9001 ggtcgttgag gtcgcggttg gacgccctgc gcgagctggt ggggctgtcg cggacccggc
     9061 tggacagccg gacgctcgca gaggcaggcc gggtactgga cgaggcggtg gcgcggcgcc
     9121 ggctgtccgg ggagcacacc gtcgtcgcca tcgcgggcgc gaccggcagc ggcaagtccc
     9181 agctcttcaa cgtgctcgcc ggagtgacca tttcggaaac cggcgtacgc aggccgacca
     9241 ccgcggcgcc catcgcgtgc agctggagcg acggcgcggc cccgctcatc gaccggctcg
     9301 gcatccccgg acggctgcgc aggcgccccc tccagagccc tgaaggagag gcacaactgc
     9361 gcgggctcgt cctggtggac ctgcccgacc acgattcggc ggccgtgcag caccgcgaac
     9421 aggtcgaccg gatcctggcg ctcgtcgacg cggtcatctg ggtcgtcgac ccggagaagt
     9481 acgcggacgc cgtcctccat gagcgctatc tgcggcccat ggcgggacac gccgaggtca
     9541 ccttcgtcgt cctcaaccag gtggaccggc tgcccggcga ggccgcacac caggttctcg
     9601 acgacctcag gaggctgctc gacgaggacg ggatcgctct gggggagtac ggcgagccgg
     9661 gcgcgaccgt gctcgcgctg tccgccctca ccggggacgg cgtcggcgaa ctgcgcgagt
     9721 cgctcgggca gttcctggcc gagcgcgggg ccgccgcgcg ccgcatctcc gccgatgtgg
     9781 aagcggccgg tgcgcgcctg cggcccgtct atgcgaccgg tcggcgtacc ggcctcagcg
     9841 aggaggcgcg cgacgacttc gccggccggc tcgcggacgc ggtgggtgcg accgctgcgg
     9901 gcgaggccgc cgagcgcgcg tggctgcgca acgccaaccg agcgtgcggc acgccctggc
     9961 tgcggctgtg gcggtggtac caggaccgga gcgaacccac cacgggtcgc ctcagggtgg
    10021 gcgcgcccgc ggacgaggag gcgaccgccc gacagcgcgt ggagcaggcc gtacgtacgg
    10081 tggcggaccg ggccgcggta gggctgcccg cgccgtgggc acaggcggtg cgcgaggcgg
    10141 ccgtacgggg gtcgcagggg ctgccggagg cgctggacga gcttgtggtg cgtacggggt
    10201 cgccggcggg gcggccgccg cggccgggct ggtggccggt cgccgtgctc gctcaggcgt
    10261 cgatgacgct cctccaggtc ttcggcggcc tgtggctgct ggggcaggtg atcgggttca
    10321 tgtcgccgaa tctcggggtg ccggtgctgt tgatggtcgc gggcatcgtc gggggacccg
    10381 ttgtggagtg gagttgcagg ctggcggcgc gggggccggc ccggcggtac ggactcgagg
    10441 ccgagcgccg gctgagggag gcggccgggg g
//
